Uncharacterized protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AF87_LEIMA

TriTrypDb:

LmjF.35.2640 , LMJLV39_350033100 , LMJSD75_350032600

Length:

612

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AF87

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF87

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	10
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0009451	RNA modification	5	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003723	RNA binding	4	10
GO:0003824	catalytic activity	1	10
GO:0005488	binding	1	10
GO:0009982	pseudouridine synthase activity	4	10
GO:0016853	isomerase activity	2	10
GO:0016866	intramolecular transferase activity	3	10
GO:0097159	organic cyclic compound binding	2	10
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	462	466	PF00656	0.484
CLV_NRD_NRD_1	11	13	PF00675	0.528
CLV_NRD_NRD_1	232	234	PF00675	0.562
CLV_NRD_NRD_1	244	246	PF00675	0.514
CLV_NRD_NRD_1	271	273	PF00675	0.482
CLV_NRD_NRD_1	313	315	PF00675	0.581
CLV_NRD_NRD_1	42	44	PF00675	0.512
CLV_NRD_NRD_1	428	430	PF00675	0.551
CLV_NRD_NRD_1	543	545	PF00675	0.505
CLV_NRD_NRD_1	560	562	PF00675	0.399
CLV_NRD_NRD_1	568	570	PF00675	0.396
CLV_PCSK_KEX2_1	232	234	PF00082	0.562
CLV_PCSK_KEX2_1	244	246	PF00082	0.374
CLV_PCSK_KEX2_1	271	273	PF00082	0.507
CLV_PCSK_KEX2_1	313	315	PF00082	0.581
CLV_PCSK_KEX2_1	42	44	PF00082	0.512
CLV_PCSK_KEX2_1	428	430	PF00082	0.495
CLV_PCSK_KEX2_1	509	511	PF00082	0.495
CLV_PCSK_KEX2_1	543	545	PF00082	0.449
CLV_PCSK_PC1ET2_1	509	511	PF00082	0.515
CLV_PCSK_SKI1_1	225	229	PF00082	0.376
CLV_PCSK_SKI1_1	244	248	PF00082	0.446
CLV_PCSK_SKI1_1	374	378	PF00082	0.531
CLV_PCSK_SKI1_1	388	392	PF00082	0.561
CLV_PCSK_SKI1_1	429	433	PF00082	0.496
CLV_PCSK_SKI1_1	488	492	PF00082	0.450
CLV_PCSK_SKI1_1	49	53	PF00082	0.370
CLV_PCSK_SKI1_1	561	565	PF00082	0.392
CLV_PCSK_SKI1_1	592	596	PF00082	0.350
DEG_ODPH_VHL_1	485	497	PF01847	0.369
DOC_CYCLIN_RxL_1	330	340	PF00134	0.419
DOC_MAPK_DCC_7	330	338	PF00069	0.363
DOC_MAPK_gen_1	268	277	PF00069	0.384
DOC_MAPK_gen_1	357	367	PF00069	0.517
DOC_MAPK_gen_1	75	84	PF00069	0.418
DOC_MAPK_gen_1	99	108	PF00069	0.413
DOC_MAPK_MEF2A_6	488	497	PF00069	0.366
DOC_MAPK_MEF2A_6	75	84	PF00069	0.406
DOC_MAPK_RevD_3	218	233	PF00069	0.391
DOC_MAPK_RevD_3	495	510	PF00069	0.358
DOC_PP2B_LxvP_1	300	303	PF13499	0.401
DOC_PP4_FxxP_1	110	113	PF00568	0.321
DOC_PP4_FxxP_1	564	567	PF00568	0.396
DOC_PP4_FxxP_1	579	582	PF00568	0.285
DOC_USP7_MATH_1	267	271	PF00917	0.437
DOC_USP7_MATH_1	352	356	PF00917	0.308
DOC_USP7_MATH_1	434	438	PF00917	0.515
DOC_USP7_MATH_1	582	586	PF00917	0.377
DOC_USP7_MATH_1	65	69	PF00917	0.492
DOC_USP7_MATH_1	76	80	PF00917	0.340
DOC_USP7_UBL2_3	562	566	PF12436	0.499
DOC_WW_Pin1_4	109	114	PF00397	0.376
DOC_WW_Pin1_4	154	159	PF00397	0.389
DOC_WW_Pin1_4	263	268	PF00397	0.434
DOC_WW_Pin1_4	536	541	PF00397	0.337
DOC_WW_Pin1_4	598	603	PF00397	0.441
DOC_WW_Pin1_4	67	72	PF00397	0.515
LIG_14-3-3_CanoR_1	180	186	PF00244	0.387
LIG_14-3-3_CanoR_1	244	253	PF00244	0.460
LIG_14-3-3_CanoR_1	440	447	PF00244	0.463
LIG_14-3-3_CanoR_1	522	528	PF00244	0.523
LIG_14-3-3_CanoR_1	77	81	PF00244	0.506
LIG_14-3-3_CanoR_1	83	90	PF00244	0.430
LIG_Actin_WH2_2	185	202	PF00022	0.503
LIG_APCC_ABBAyCdc20_2	524	530	PF00400	0.358
LIG_BIR_III_2	73	77	PF00653	0.421
LIG_BRCT_BRCA1_1	137	141	PF00533	0.431
LIG_BRCT_BRCA1_1	499	503	PF00533	0.343
LIG_BRCT_BRCA1_1	602	606	PF00533	0.442
LIG_BRCT_BRCA1_1	62	66	PF00533	0.505
LIG_CtBP_PxDLS_1	498	502	PF00389	0.358
LIG_deltaCOP1_diTrp_1	409	413	PF00928	0.425
LIG_eIF4E_1	224	230	PF01652	0.438
LIG_FHA_1	180	186	PF00498	0.409
LIG_FHA_1	248	254	PF00498	0.430
LIG_FHA_1	362	368	PF00498	0.397
LIG_FHA_1	440	446	PF00498	0.488
LIG_FHA_1	586	592	PF00498	0.437
LIG_FHA_1	76	82	PF00498	0.440
LIG_FHA_2	430	436	PF00498	0.481
LIG_FHA_2	68	74	PF00498	0.509
LIG_FHA_2	90	96	PF00498	0.450
LIG_Integrin_RGD_1	556	558	PF01839	0.425
LIG_LIR_Apic_2	153	158	PF02991	0.450
LIG_LIR_Gen_1	121	131	PF02991	0.386
LIG_LIR_Gen_1	273	281	PF02991	0.467
LIG_LIR_Gen_1	347	356	PF02991	0.318
LIG_LIR_Gen_1	409	418	PF02991	0.479
LIG_LIR_Gen_1	465	475	PF02991	0.349
LIG_LIR_LC3C_4	78	82	PF02991	0.368
LIG_LIR_Nem_3	121	126	PF02991	0.325
LIG_LIR_Nem_3	234	238	PF02991	0.331
LIG_LIR_Nem_3	273	277	PF02991	0.351
LIG_LIR_Nem_3	347	353	PF02991	0.322
LIG_LIR_Nem_3	409	413	PF02991	0.466
LIG_LIR_Nem_3	465	471	PF02991	0.354
LIG_LIR_Nem_3	500	506	PF02991	0.344
LIG_LIR_Nem_3	603	609	PF02991	0.465
LIG_MYND_1	173	177	PF01753	0.397
LIG_PCNA_yPIPBox_3	186	196	PF02747	0.475
LIG_PCNA_yPIPBox_3	433	445	PF02747	0.398
LIG_REV1ctd_RIR_1	561	566	PF16727	0.479
LIG_SH2_CRK	427	431	PF00017	0.409
LIG_SH2_NCK_1	210	214	PF00017	0.295
LIG_SH2_NCK_1	32	36	PF00017	0.480
LIG_SH2_PTP2	123	126	PF00017	0.412
LIG_SH2_PTP2	155	158	PF00017	0.371
LIG_SH2_SRC	32	35	PF00017	0.453
LIG_SH2_STAP1	181	185	PF00017	0.346
LIG_SH2_STAP1	210	214	PF00017	0.331
LIG_SH2_STAT3	528	531	PF00017	0.391
LIG_SH2_STAT5	123	126	PF00017	0.330
LIG_SH2_STAT5	155	158	PF00017	0.459
LIG_SH2_STAT5	181	184	PF00017	0.463
LIG_SH2_STAT5	222	225	PF00017	0.479
LIG_SH2_STAT5	504	507	PF00017	0.333
LIG_SH2_STAT5	55	58	PF00017	0.350
LIG_SH3_3	441	447	PF00018	0.352
LIG_SH3_3	492	498	PF00018	0.392
LIG_SH3_4	566	573	PF00018	0.431
LIG_SUMO_SIM_anti_2	273	279	PF11976	0.368
LIG_SUMO_SIM_par_1	334	340	PF11976	0.473
LIG_SUMO_SIM_par_1	363	369	PF11976	0.505
LIG_TRAF2_1	158	161	PF00917	0.514
LIG_TRFH_1	563	567	PF08558	0.470
LIG_TYR_ITIM	425	430	PF00017	0.343
LIG_UBA3_1	605	612	PF00899	0.364
LIG_WRC_WIRS_1	446	451	PF05994	0.442
MOD_CDK_SPK_2	263	268	PF00069	0.514
MOD_CDK_SPxxK_3	536	543	PF00069	0.451
MOD_CK1_1	118	124	PF00069	0.507
MOD_CK1_1	266	272	PF00069	0.468
MOD_CK1_1	411	417	PF00069	0.545
MOD_CK1_1	460	466	PF00069	0.664
MOD_CK1_1	539	545	PF00069	0.484
MOD_CK1_1	585	591	PF00069	0.419
MOD_CK2_1	273	279	PF00069	0.489
MOD_CK2_1	429	435	PF00069	0.342
MOD_CK2_1	89	95	PF00069	0.389
MOD_GlcNHglycan	165	169	PF01048	0.469
MOD_GlcNHglycan	324	327	PF01048	0.365
MOD_GlcNHglycan	404	407	PF01048	0.451
MOD_GlcNHglycan	409	413	PF01048	0.493
MOD_GlcNHglycan	588	591	PF01048	0.389
MOD_GlcNHglycan	602	605	PF01048	0.373
MOD_GSK3_1	175	182	PF00069	0.532
MOD_GSK3_1	21	28	PF00069	0.390
MOD_GSK3_1	244	251	PF00069	0.443
MOD_GSK3_1	263	270	PF00069	0.300
MOD_GSK3_1	457	464	PF00069	0.536
MOD_GSK3_1	582	589	PF00069	0.335
MOD_GSK3_1	596	603	PF00069	0.296
MOD_N-GLC_1	208	213	PF02516	0.306
MOD_NEK2_1	137	142	PF00069	0.538
MOD_NEK2_1	146	151	PF00069	0.299
MOD_NEK2_1	208	213	PF00069	0.348
MOD_NEK2_1	238	243	PF00069	0.370
MOD_NEK2_1	402	407	PF00069	0.509
MOD_NEK2_1	571	576	PF00069	0.430
MOD_NEK2_1	577	582	PF00069	0.379
MOD_NEK2_1	596	601	PF00069	0.332
MOD_NEK2_1	66	71	PF00069	0.474
MOD_NEK2_2	181	186	PF00069	0.406
MOD_NEK2_2	352	357	PF00069	0.319
MOD_PIKK_1	434	440	PF00454	0.396
MOD_PKA_1	244	250	PF00069	0.406
MOD_PKA_2	179	185	PF00069	0.340
MOD_PKA_2	244	250	PF00069	0.485
MOD_PKA_2	267	273	PF00069	0.509
MOD_PKA_2	295	301	PF00069	0.468
MOD_PKA_2	439	445	PF00069	0.439
MOD_PKA_2	542	548	PF00069	0.583
MOD_PKA_2	76	82	PF00069	0.432
MOD_PKA_2	8	14	PF00069	0.486
MOD_PKB_1	372	380	PF00069	0.519
MOD_Plk_1	21	27	PF00069	0.483
MOD_Plk_1	408	414	PF00069	0.554
MOD_Plk_1	434	440	PF00069	0.396
MOD_Plk_2-3	273	279	PF00069	0.477
MOD_Plk_4	238	244	PF00069	0.336
MOD_Plk_4	273	279	PF00069	0.558
MOD_Plk_4	361	367	PF00069	0.295
MOD_Plk_4	499	505	PF00069	0.438
MOD_Plk_4	523	529	PF00069	0.476
MOD_Plk_4	76	82	PF00069	0.495
MOD_Plk_4	89	95	PF00069	0.389
MOD_ProDKin_1	109	115	PF00069	0.376
MOD_ProDKin_1	154	160	PF00069	0.396
MOD_ProDKin_1	263	269	PF00069	0.426
MOD_ProDKin_1	536	542	PF00069	0.336
MOD_ProDKin_1	598	604	PF00069	0.438
MOD_ProDKin_1	67	73	PF00069	0.522
MOD_SUMO_rev_2	68	76	PF00179	0.495
TRG_AP2beta_CARGO_1	347	357	PF09066	0.342
TRG_DiLeu_BaEn_2	233	239	PF01217	0.430
TRG_DiLeu_BaLyEn_6	454	459	PF01217	0.417
TRG_DiLeu_BaLyEn_6	604	609	PF01217	0.343
TRG_DiLeu_BaLyEn_6	80	85	PF01217	0.458
TRG_ENDOCYTIC_2	123	126	PF00928	0.412
TRG_ENDOCYTIC_2	235	238	PF00928	0.346
TRG_ENDOCYTIC_2	427	430	PF00928	0.544
TRG_ER_diArg_1	231	233	PF00400	0.396
TRG_ER_diArg_1	243	245	PF00400	0.308
TRG_ER_diArg_1	312	314	PF00400	0.559
TRG_ER_diArg_1	371	374	PF00400	0.512
TRG_ER_diArg_1	42	44	PF00400	0.374
TRG_ER_diArg_1	427	429	PF00400	0.484
TRG_NES_CRM1_1	465	478	PF08389	0.401
TRG_Pf-PMV_PEXEL_1	200	204	PF00026	0.377
TRG_Pf-PMV_PEXEL_1	244	248	PF00026	0.408
TRG_Pf-PMV_PEXEL_1	334	339	PF00026	0.365
TRG_Pf-PMV_PEXEL_1	374	379	PF00026	0.618
TRG_Pf-PMV_PEXEL_1	492	496	PF00026	0.427
TRG_Pf-PMV_PEXEL_1	83	87	PF00026	0.458
TRG_Pf-PMV_PEXEL_1	96	100	PF00026	0.503

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8H8	Leptomonas seymouri	64%	98%
A0A0S4JFS5	Bodo saltans	31%	92%
A0A1X0P5I6	Trypanosomatidae	41%	100%
A0A3Q8IJH4	Leishmania donovani	95%	100%
A0A3S5ISL3	Trypanosoma rangeli	44%	100%
A4IBD1	Leishmania infantum	94%	100%
C9ZZ88	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9B6E2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%