Uncharacterized protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

PHD-like zinc-binding domain containing protein, putative

Species:

Leishmania major

UniProt:

E9AF86_LEIMA

TriTrypDb:

LmjF.35.2630 , LMJLV39_350033000 , LMJSD75_350032500

Length:

743

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AF86

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF86

Function

Biological processes
Term	Name	Level	Count
GO:0006355	regulation of DNA-templated transcription	6	2
GO:0006357	regulation of transcription by RNA polymerase II	7	2
GO:0009889	regulation of biosynthetic process	4	2
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0080090	regulation of primary metabolic process	4	2
GO:1903506	regulation of nucleic acid-templated transcription	7	2
GO:2001141	regulation of RNA biosynthetic process	6	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	292	296	PF00656	0.495
CLV_C14_Caspase3-7	738	742	PF00656	0.662
CLV_NRD_NRD_1	324	326	PF00675	0.452
CLV_NRD_NRD_1	336	338	PF00675	0.402
CLV_NRD_NRD_1	446	448	PF00675	0.666
CLV_NRD_NRD_1	525	527	PF00675	0.530
CLV_NRD_NRD_1	531	533	PF00675	0.504
CLV_NRD_NRD_1	586	588	PF00675	0.575
CLV_NRD_NRD_1	88	90	PF00675	0.530
CLV_PCSK_FUR_1	529	533	PF00082	0.510
CLV_PCSK_KEX2_1	324	326	PF00082	0.468
CLV_PCSK_KEX2_1	336	338	PF00082	0.468
CLV_PCSK_KEX2_1	446	448	PF00082	0.664
CLV_PCSK_KEX2_1	531	533	PF00082	0.527
CLV_PCSK_KEX2_1	579	581	PF00082	0.510
CLV_PCSK_KEX2_1	585	587	PF00082	0.561
CLV_PCSK_KEX2_1	88	90	PF00082	0.530
CLV_PCSK_KEX2_1	98	100	PF00082	0.628
CLV_PCSK_PC1ET2_1	579	581	PF00082	0.556
CLV_PCSK_PC1ET2_1	98	100	PF00082	0.764
CLV_PCSK_PC7_1	84	90	PF00082	0.532
CLV_PCSK_PC7_1	94	100	PF00082	0.683
CLV_PCSK_SKI1_1	11	15	PF00082	0.529
CLV_PCSK_SKI1_1	257	261	PF00082	0.558
CLV_PCSK_SKI1_1	325	329	PF00082	0.496
CLV_PCSK_SKI1_1	89	93	PF00082	0.572
CLV_Separin_Metazoa	363	367	PF03568	0.514
DEG_APCC_DBOX_1	198	206	PF00400	0.674
DEG_SPOP_SBC_1	435	439	PF00917	0.699
DEG_SPOP_SBC_1	459	463	PF00917	0.535
DOC_ANK_TNKS_1	585	592	PF00023	0.634
DOC_CKS1_1	368	373	PF01111	0.533
DOC_CYCLIN_yCln2_LP_2	259	265	PF00134	0.343
DOC_MAPK_gen_1	22	30	PF00069	0.312
DOC_MAPK_gen_1	280	288	PF00069	0.466
DOC_MAPK_gen_1	3	12	PF00069	0.438
DOC_MAPK_gen_1	321	330	PF00069	0.468
DOC_MAPK_gen_1	336	343	PF00069	0.457
DOC_MAPK_gen_1	355	364	PF00069	0.273
DOC_MAPK_gen_1	479	486	PF00069	0.645
DOC_MAPK_gen_1	526	536	PF00069	0.670
DOC_MAPK_JIP1_4	99	105	PF00069	0.532
DOC_MAPK_MEF2A_6	271	279	PF00069	0.377
DOC_MAPK_MEF2A_6	280	288	PF00069	0.480
DOC_MAPK_MEF2A_6	3	12	PF00069	0.415
DOC_MAPK_NFAT4_5	5	13	PF00069	0.421
DOC_PP1_RVXF_1	7	13	PF00149	0.433
DOC_PP2B_LxvP_1	133	136	PF13499	0.565
DOC_PP4_FxxP_1	118	121	PF00568	0.664
DOC_PP4_FxxP_1	279	282	PF00568	0.477
DOC_PP4_FxxP_1	30	33	PF00568	0.514
DOC_SPAK_OSR1_1	158	162	PF12202	0.364
DOC_SPAK_OSR1_1	278	282	PF12202	0.338
DOC_USP7_MATH_1	240	244	PF00917	0.623
DOC_USP7_MATH_1	415	419	PF00917	0.660
DOC_USP7_MATH_1	434	438	PF00917	0.633
DOC_USP7_MATH_1	459	463	PF00917	0.691
DOC_USP7_MATH_1	464	468	PF00917	0.632
DOC_USP7_MATH_1	491	495	PF00917	0.567
DOC_USP7_MATH_1	624	628	PF00917	0.570
DOC_USP7_MATH_1	723	727	PF00917	0.614
DOC_WW_Pin1_4	111	116	PF00397	0.755
DOC_WW_Pin1_4	187	192	PF00397	0.756
DOC_WW_Pin1_4	223	228	PF00397	0.692
DOC_WW_Pin1_4	367	372	PF00397	0.498
DOC_WW_Pin1_4	492	497	PF00397	0.562
LIG_14-3-3_CanoR_1	446	451	PF00244	0.646
LIG_14-3-3_CanoR_1	660	668	PF00244	0.509
LIG_14-3-3_CanoR_1	693	701	PF00244	0.603
LIG_Actin_WH2_2	391	408	PF00022	0.550
LIG_AP2alpha_2	683	685	PF02296	0.613
LIG_Clathr_ClatBox_1	353	357	PF01394	0.450
LIG_FHA_1	258	264	PF00498	0.474
LIG_FHA_1	298	304	PF00498	0.507
LIG_FHA_1	461	467	PF00498	0.722
LIG_FHA_1	536	542	PF00498	0.443
LIG_FHA_1	593	599	PF00498	0.541
LIG_FHA_1	606	612	PF00498	0.399
LIG_FHA_2	138	144	PF00498	0.648
LIG_FHA_2	285	291	PF00498	0.455
LIG_FHA_2	305	311	PF00498	0.504
LIG_FHA_2	380	386	PF00498	0.636
LIG_FHA_2	493	499	PF00498	0.639
LIG_FHA_2	503	509	PF00498	0.569
LIG_FHA_2	99	105	PF00498	0.725
LIG_Integrin_RGD_1	321	323	PF01839	0.510
LIG_LIR_Apic_2	116	121	PF02991	0.652
LIG_LIR_Apic_2	29	33	PF02991	0.517
LIG_LIR_Apic_2	683	689	PF02991	0.594
LIG_LIR_Gen_1	307	316	PF02991	0.513
LIG_LIR_Gen_1	35	45	PF02991	0.442
LIG_LIR_Gen_1	57	64	PF02991	0.439
LIG_LIR_Gen_1	662	672	PF02991	0.423
LIG_LIR_Gen_1	673	684	PF02991	0.506
LIG_LIR_Gen_1	76	87	PF02991	0.545
LIG_LIR_Nem_3	21	27	PF02991	0.521
LIG_LIR_Nem_3	307	311	PF02991	0.519
LIG_LIR_Nem_3	35	41	PF02991	0.345
LIG_LIR_Nem_3	429	435	PF02991	0.736
LIG_LIR_Nem_3	57	62	PF02991	0.441
LIG_LIR_Nem_3	662	668	PF02991	0.422
LIG_LIR_Nem_3	673	679	PF02991	0.492
LIG_LIR_Nem_3	76	82	PF02991	0.398
LIG_LYPXL_yS_3	432	435	PF13949	0.680
LIG_PTB_Apo_2	117	124	PF02174	0.664
LIG_PTB_Phospho_1	117	123	PF10480	0.661
LIG_SH2_GRB2like	519	522	PF00017	0.452
LIG_SH2_GRB2like	676	679	PF00017	0.499
LIG_SH2_PTP2	676	679	PF00017	0.401
LIG_SH2_STAT5	269	272	PF00017	0.464
LIG_SH2_STAT5	334	337	PF00017	0.423
LIG_SH2_STAT5	676	679	PF00017	0.499
LIG_SH2_STAT5	78	81	PF00017	0.479
LIG_SH3_1	94	100	PF00018	0.659
LIG_SH3_3	10	16	PF00018	0.487
LIG_SH3_3	493	499	PF00018	0.618
LIG_SH3_3	610	616	PF00018	0.497
LIG_SH3_3	681	687	PF00018	0.547
LIG_SH3_3	94	100	PF00018	0.659
LIG_SUMO_SIM_anti_2	695	701	PF11976	0.648
LIG_SUMO_SIM_par_1	379	385	PF11976	0.674
LIG_SUMO_SIM_par_1	391	396	PF11976	0.687
LIG_TRAF2_1	480	483	PF00917	0.595
LIG_UBA3_1	352	361	PF00899	0.513
LIG_WRC_WIRS_1	27	32	PF05994	0.568
MOD_CDC14_SPxK_1	226	229	PF00782	0.679
MOD_CDK_SPxK_1	223	229	PF00069	0.683
MOD_CK1_1	138	144	PF00069	0.726
MOD_CK1_1	165	171	PF00069	0.650
MOD_CK1_1	182	188	PF00069	0.595
MOD_CK1_1	213	219	PF00069	0.531
MOD_CK1_1	413	419	PF00069	0.653
MOD_CK1_1	437	443	PF00069	0.668
MOD_CK1_1	548	554	PF00069	0.506
MOD_CK1_1	57	63	PF00069	0.470
MOD_CK2_1	26	32	PF00069	0.567
MOD_CK2_1	284	290	PF00069	0.430
MOD_CK2_1	304	310	PF00069	0.493
MOD_CK2_1	357	363	PF00069	0.448
MOD_CK2_1	379	385	PF00069	0.636
MOD_CK2_1	413	419	PF00069	0.748
MOD_CK2_1	477	483	PF00069	0.623
MOD_CK2_1	492	498	PF00069	0.648
MOD_CK2_1	624	630	PF00069	0.574
MOD_CK2_1	713	719	PF00069	0.607
MOD_CK2_1	98	104	PF00069	0.660
MOD_GlcNHglycan	128	131	PF01048	0.794
MOD_GlcNHglycan	184	187	PF01048	0.660
MOD_GlcNHglycan	212	215	PF01048	0.718
MOD_GlcNHglycan	219	222	PF01048	0.688
MOD_GlcNHglycan	376	379	PF01048	0.666
MOD_GlcNHglycan	388	391	PF01048	0.728
MOD_GlcNHglycan	408	411	PF01048	0.786
MOD_GlcNHglycan	412	415	PF01048	0.735
MOD_GlcNHglycan	424	427	PF01048	0.565
MOD_GlcNHglycan	428	431	PF01048	0.447
MOD_GlcNHglycan	442	445	PF01048	0.644
MOD_GlcNHglycan	455	458	PF01048	0.546
MOD_GlcNHglycan	466	469	PF01048	0.602
MOD_GlcNHglycan	549	553	PF01048	0.565
MOD_GlcNHglycan	605	608	PF01048	0.551
MOD_GlcNHglycan	622	625	PF01048	0.547
MOD_GlcNHglycan	626	629	PF01048	0.596
MOD_GlcNHglycan	672	675	PF01048	0.513
MOD_GlcNHglycan	79	82	PF01048	0.527
MOD_GSK3_1	131	138	PF00069	0.753
MOD_GSK3_1	213	220	PF00069	0.705
MOD_GSK3_1	297	304	PF00069	0.454
MOD_GSK3_1	406	413	PF00069	0.721
MOD_GSK3_1	422	429	PF00069	0.546
MOD_GSK3_1	434	441	PF00069	0.552
MOD_GSK3_1	448	455	PF00069	0.668
MOD_GSK3_1	460	467	PF00069	0.592
MOD_GSK3_1	599	606	PF00069	0.505
MOD_GSK3_1	620	627	PF00069	0.536
MOD_GSK3_1	73	80	PF00069	0.570
MOD_N-GLC_1	138	143	PF02516	0.688
MOD_N-GLC_2	642	644	PF02516	0.517
MOD_NEK2_1	163	168	PF00069	0.674
MOD_NEK2_1	180	185	PF00069	0.607
MOD_NEK2_1	219	224	PF00069	0.701
MOD_NEK2_1	356	361	PF00069	0.521
MOD_NEK2_1	452	457	PF00069	0.735
MOD_NEK2_1	52	57	PF00069	0.484
MOD_NEK2_1	561	566	PF00069	0.570
MOD_NEK2_1	603	608	PF00069	0.476
MOD_NEK2_2	379	384	PF00069	0.717
MOD_NEK2_2	607	612	PF00069	0.545
MOD_PIKK_1	297	303	PF00454	0.519
MOD_PIKK_1	393	399	PF00454	0.659
MOD_PIKK_1	535	541	PF00454	0.492
MOD_PIKK_1	692	698	PF00454	0.539
MOD_PKA_1	446	452	PF00069	0.752
MOD_PKA_1	98	104	PF00069	0.724
MOD_PKA_2	357	363	PF00069	0.482
MOD_PKA_2	445	451	PF00069	0.666
MOD_PKA_2	562	568	PF00069	0.636
MOD_PKA_2	659	665	PF00069	0.512
MOD_PKA_2	692	698	PF00069	0.572
MOD_PKA_2	98	104	PF00069	0.724
MOD_PKB_1	255	263	PF00069	0.494
MOD_PKB_1	404	412	PF00069	0.681
MOD_Plk_1	257	263	PF00069	0.434
MOD_Plk_1	34	40	PF00069	0.486
MOD_Plk_1	393	399	PF00069	0.675
MOD_Plk_1	57	63	PF00069	0.436
MOD_Plk_1	637	643	PF00069	0.512
MOD_Plk_1	713	719	PF00069	0.609
MOD_Plk_2-3	26	32	PF00069	0.465
MOD_Plk_2-3	34	40	PF00069	0.486
MOD_Plk_4	599	605	PF00069	0.540
MOD_ProDKin_1	111	117	PF00069	0.750
MOD_ProDKin_1	187	193	PF00069	0.759
MOD_ProDKin_1	223	229	PF00069	0.694
MOD_ProDKin_1	367	373	PF00069	0.500
MOD_ProDKin_1	492	498	PF00069	0.563
MOD_SUMO_rev_2	40	49	PF00179	0.465
MOD_SUMO_rev_2	584	594	PF00179	0.635
TRG_DiLeu_BaLyEn_6	348	353	PF01217	0.444
TRG_DiLeu_BaLyEn_6	593	598	PF01217	0.543
TRG_DiLeu_LyEn_5	511	516	PF01217	0.459
TRG_ENDOCYTIC_2	38	41	PF00928	0.435
TRG_ENDOCYTIC_2	432	435	PF00928	0.680
TRG_ENDOCYTIC_2	676	679	PF00928	0.543
TRG_ER_diArg_1	2	5	PF00400	0.468
TRG_ER_diArg_1	254	257	PF00400	0.486
TRG_ER_diArg_1	335	337	PF00400	0.480
TRG_ER_diArg_1	445	447	PF00400	0.739
TRG_ER_diArg_1	478	481	PF00400	0.529
TRG_ER_diArg_1	528	531	PF00400	0.611
TRG_ER_diArg_1	585	587	PF00400	0.575
TRG_ER_diArg_1	8	11	PF00400	0.461
TRG_Pf-PMV_PEXEL_1	42	46	PF00026	0.518
TRG_Pf-PMV_PEXEL_1	580	584	PF00026	0.550

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDF9	Leptomonas seymouri	69%	100%
A0A3S7X9C0	Leishmania donovani	92%	100%
A4HMT2	Leishmania braziliensis	81%	100%
A4IBD0	Leishmania infantum	92%	100%
C9ZZ89	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9B6E1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
V5DUV4	Trypanosoma cruzi	49%	100%