Palmitoyltransferase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DHHC palmitoyltransferase, putative

Species:

Leishmania major

UniProt:

E9AF82_LEIMA

TriTrypDb:

LmjF.35.2590 , LMJLV39_350032600 , LMJSD75_350032100

Length:

280

Annotations

LeishMANIAdb annotations

Related to many other eukaryotic palmitoyltransferases (e.g. mammalian ZDHHC4/9/14/24)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0005794	Golgi apparatus	5	2
GO:0016020	membrane	2	12
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9AF82

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF82

Function

Biological processes
Term	Name	Level	Count
GO:0006497	protein lipidation	5	2
GO:0006605	protein targeting	5	2
GO:0006612	protein targeting to membrane	5	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0008104	protein localization	4	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018198	peptidyl-cysteine modification	6	2
GO:0018230	peptidyl-L-cysteine S-palmitoylation	7	2
GO:0018231	peptidyl-S-diacylglycerol-L-cysteine biosynthetic process from peptidyl-cysteine	7	2
GO:0018345	protein palmitoylation	6	2
GO:0019538	protein metabolic process	3	2
GO:0033036	macromolecule localization	2	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043543	protein acylation	5	2
GO:0044238	primary metabolic process	2	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0051668	localization within membrane	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071704	organic substance metabolic process	2	2
GO:0071705	nitrogen compound transport	4	2
GO:0072657	protein localization to membrane	4	2
GO:0090150	establishment of protein localization to membrane	4	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0016409	palmitoyltransferase activity	5	12
GO:0016417	S-acyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016746	acyltransferase activity	3	12
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	12
GO:0019706	protein-cysteine S-palmitoyltransferase activity	4	12
GO:0019707	protein-cysteine S-acyltransferase activity	3	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	263	265	PF00675	0.368
CLV_PCSK_KEX2_1	146	148	PF00082	0.208
CLV_PCSK_KEX2_1	263	265	PF00082	0.335
CLV_PCSK_KEX2_1	9	11	PF00082	0.365
CLV_PCSK_PC1ET2_1	146	148	PF00082	0.224
CLV_PCSK_PC1ET2_1	9	11	PF00082	0.365
CLV_PCSK_SKI1_1	263	267	PF00082	0.326
CLV_PCSK_SKI1_1	9	13	PF00082	0.378
DEG_APCC_DBOX_1	184	192	PF00400	0.211
DOC_CDC14_PxL_1	32	40	PF14671	0.284
DOC_CYCLIN_RxL_1	260	267	PF00134	0.455
DOC_MAPK_gen_1	253	261	PF00069	0.427
DOC_MAPK_gen_1	9	17	PF00069	0.668
DOC_PP1_RVXF_1	14	20	PF00149	0.654
DOC_PP2B_LxvP_1	208	211	PF13499	0.284
DOC_USP7_MATH_1	221	225	PF00917	0.438
DOC_USP7_MATH_1	91	95	PF00917	0.632
DOC_WW_Pin1_4	264	269	PF00397	0.626
LIG_14-3-3_CanoR_1	116	125	PF00244	0.424
LIG_14-3-3_CanoR_1	234	243	PF00244	0.581
LIG_BRCT_BRCA1_1	175	179	PF00533	0.211
LIG_deltaCOP1_diTrp_1	26	32	PF00928	0.420
LIG_deltaCOP1_diTrp_1	53	59	PF00928	0.238
LIG_eIF4E_1	57	63	PF01652	0.360
LIG_FHA_1	109	115	PF00498	0.429
LIG_FHA_1	157	163	PF00498	0.265
LIG_FHA_1	194	200	PF00498	0.283
LIG_FHA_1	40	46	PF00498	0.349
LIG_FHA_1	63	69	PF00498	0.321
LIG_FHA_1	70	76	PF00498	0.331
LIG_FHA_2	223	229	PF00498	0.392
LIG_GBD_Chelix_1	200	208	PF00786	0.377
LIG_LIR_Gen_1	159	168	PF02991	0.339
LIG_LIR_Gen_1	170	179	PF02991	0.379
LIG_LIR_Gen_1	26	36	PF02991	0.495
LIG_LIR_Gen_1	41	50	PF02991	0.158
LIG_LIR_Gen_1	56	66	PF02991	0.147
LIG_LIR_Nem_3	148	152	PF02991	0.402
LIG_LIR_Nem_3	159	164	PF02991	0.236
LIG_LIR_Nem_3	170	174	PF02991	0.379
LIG_LIR_Nem_3	26	31	PF02991	0.536
LIG_LIR_Nem_3	41	47	PF02991	0.148
LIG_LIR_Nem_3	53	57	PF02991	0.155
LIG_LIR_Nem_3	60	66	PF02991	0.252
LIG_PDZ_Class_2	275	280	PF00595	0.584
LIG_Pex14_1	28	32	PF04695	0.447
LIG_Pex14_2	151	155	PF04695	0.247
LIG_Pex14_2	40	44	PF04695	0.402
LIG_Pex14_2	59	63	PF04695	0.458
LIG_SH2_PTP2	165	168	PF00017	0.326
LIG_SH2_PTP2	216	219	PF00017	0.442
LIG_SH2_STAT5	165	168	PF00017	0.305
LIG_SH2_STAT5	216	219	PF00017	0.437
LIG_SH2_STAT5	257	260	PF00017	0.521
LIG_SH2_STAT5	57	60	PF00017	0.281
LIG_SH3_3	30	36	PF00018	0.284
LIG_SH3_3	44	50	PF00018	0.349
LIG_SH3_3	78	84	PF00018	0.484
LIG_SUMO_SIM_anti_2	170	178	PF11976	0.360
LIG_SUMO_SIM_par_1	170	178	PF11976	0.349
LIG_SUMO_SIM_par_1	64	69	PF11976	0.348
LIG_SUMO_SIM_par_1	71	76	PF11976	0.382
LIG_TYR_ITIM	163	168	PF00017	0.247
LIG_WRC_WIRS_1	158	163	PF05994	0.460
LIG_WRC_WIRS_1	168	173	PF05994	0.198
LIG_WW_3	116	120	PF00397	0.411
MOD_CDK_SPxxK_3	264	271	PF00069	0.533
MOD_CK1_1	222	228	PF00069	0.480
MOD_CK2_1	222	228	PF00069	0.392
MOD_Cter_Amidation	144	147	PF01082	0.306
MOD_GlcNHglycan	219	222	PF01048	0.282
MOD_GlcNHglycan	238	241	PF01048	0.308
MOD_GlcNHglycan	253	256	PF01048	0.262
MOD_GSK3_1	217	224	PF00069	0.460
MOD_GSK3_1	39	46	PF00069	0.405
MOD_GSK3_1	53	60	PF00069	0.141
MOD_GSK3_1	62	69	PF00069	0.283
MOD_GSK3_1	85	92	PF00069	0.539
MOD_N-GLC_1	222	227	PF02516	0.202
MOD_NEK2_1	156	161	PF00069	0.265
MOD_NEK2_1	167	172	PF00069	0.160
MOD_NEK2_1	191	196	PF00069	0.230
MOD_NEK2_1	219	224	PF00069	0.445
MOD_NEK2_1	38	43	PF00069	0.360
MOD_NEK2_1	62	67	PF00069	0.280
MOD_NEK2_1	71	76	PF00069	0.258
MOD_NEK2_2	145	150	PF00069	0.411
MOD_OFUCOSY	106	112	PF10250	0.242
MOD_OFUCOSY	137	143	PF10250	0.224
MOD_PIKK_1	229	235	PF00454	0.575
MOD_PIKK_1	85	91	PF00454	0.559
MOD_PKA_2	118	124	PF00069	0.411
MOD_Plk_1	222	228	PF00069	0.497
MOD_Plk_4	157	163	PF00069	0.303
MOD_Plk_4	39	45	PF00069	0.341
MOD_Plk_4	53	59	PF00069	0.131
MOD_ProDKin_1	264	270	PF00069	0.629
MOD_SUMO_rev_2	176	182	PF00179	0.211
TRG_DiLeu_BaEn_1	184	189	PF01217	0.211
TRG_ENDOCYTIC_2	165	168	PF00928	0.317
TRG_ENDOCYTIC_2	216	219	PF00928	0.421
TRG_ER_diArg_1	14	17	PF00400	0.627
TRG_ER_diArg_1	262	264	PF00400	0.575
TRG_ER_diArg_1	271	274	PF00400	0.614

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZ19	Leptomonas seymouri	25%	80%
A0A0S4INR8	Bodo saltans	30%	94%
A0A0S4IT15	Bodo saltans	29%	79%
A0A0S4IXZ6	Bodo saltans	28%	100%
A0A0S4KJ22	Bodo saltans	38%	92%
A0A0S4KJ92	Bodo saltans	27%	100%
A0A1X0NY23	Trypanosomatidae	26%	94%
A0A1X0P5Q8	Trypanosomatidae	42%	87%
A0A3Q8ICY2	Leishmania donovani	28%	79%
A0A3Q8IIC8	Leishmania donovani	95%	100%
A0A3R7N519	Trypanosoma rangeli	34%	94%
A0A3R7P4J6	Trypanosoma rangeli	32%	94%
A0A422NE16	Trypanosoma rangeli	44%	86%
A4HCH5	Leishmania braziliensis	26%	70%
A4HG63	Leishmania braziliensis	27%	78%
A4HMS9	Leishmania braziliensis	80%	100%
A4I395	Leishmania infantum	28%	79%
A4IBG8	Leishmania infantum	95%	100%
D0A7S1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	92%
E7F587	Danio rerio	23%	87%
E9AVW7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	71%
E9AZI3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	79%
E9B6D8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
O74384	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	80%
O80685	Arabidopsis thaliana	27%	68%
P0C7U3	Homo sapiens	25%	75%
Q2TGJ1	Rattus norvegicus	33%	73%
Q4Q8P8	Leishmania major	25%	79%
Q5M757	Arabidopsis thaliana	27%	96%
Q5PNZ1	Arabidopsis thaliana	25%	68%
Q5Y5T2	Mus musculus	32%	74%
Q6BHT4	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	23%	75%
Q6CQB5	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	30%	79%
Q6FSS4	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	28%	86%
Q7ZVN4	Danio rerio	32%	72%
Q8VYS8	Arabidopsis thaliana	26%	69%
Q9FLM3	Arabidopsis thaliana	30%	68%
Q9H8X9	Homo sapiens	25%	68%
Q9NUE0	Homo sapiens	30%	72%
Q9SB58	Arabidopsis thaliana	29%	69%
V5B8P9	Trypanosoma cruzi	25%	80%
V5BCH8	Trypanosoma cruzi	33%	93%
V5C316	Trypanosoma cruzi	43%	85%