Putative RNA binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Double RNA binding domain protein 9

Species:

Leishmania major

UniProt:

E9AF78_LEIMA

TriTrypDb:

LmjF.35.2550 , LMJLV39_350032200 , LMJSD75_350031700

Length:

455

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005730	nucleolus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AF78

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF78

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003723	RNA binding	4	10
GO:0005488	binding	1	10
GO:0097159	organic cyclic compound binding	2	10
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	383	387	PF00656	0.805
CLV_NRD_NRD_1	137	139	PF00675	0.519
CLV_NRD_NRD_1	360	362	PF00675	0.519
CLV_NRD_NRD_1	364	366	PF00675	0.514
CLV_NRD_NRD_1	420	422	PF00675	0.551
CLV_NRD_NRD_1	44	46	PF00675	0.481
CLV_NRD_NRD_1	447	449	PF00675	0.541
CLV_NRD_NRD_1	74	76	PF00675	0.782
CLV_PCSK_FUR_1	361	365	PF00082	0.512
CLV_PCSK_KEX2_1	137	139	PF00082	0.575
CLV_PCSK_KEX2_1	141	143	PF00082	0.577
CLV_PCSK_KEX2_1	174	176	PF00082	0.233
CLV_PCSK_KEX2_1	299	301	PF00082	0.264
CLV_PCSK_KEX2_1	360	362	PF00082	0.519
CLV_PCSK_KEX2_1	363	365	PF00082	0.512
CLV_PCSK_KEX2_1	379	381	PF00082	0.587
CLV_PCSK_KEX2_1	420	422	PF00082	0.623
CLV_PCSK_KEX2_1	44	46	PF00082	0.553
CLV_PCSK_KEX2_1	447	449	PF00082	0.563
CLV_PCSK_KEX2_1	57	59	PF00082	0.595
CLV_PCSK_PC1ET2_1	141	143	PF00082	0.620
CLV_PCSK_PC1ET2_1	174	176	PF00082	0.233
CLV_PCSK_PC1ET2_1	299	301	PF00082	0.262
CLV_PCSK_PC1ET2_1	363	365	PF00082	0.512
CLV_PCSK_PC1ET2_1	379	381	PF00082	0.587
CLV_PCSK_PC1ET2_1	447	449	PF00082	0.563
CLV_PCSK_PC1ET2_1	57	59	PF00082	0.583
CLV_PCSK_PC7_1	137	143	PF00082	0.505
CLV_PCSK_PC7_1	360	366	PF00082	0.560
CLV_PCSK_PC7_1	443	449	PF00082	0.527
CLV_PCSK_SKI1_1	115	119	PF00082	0.609
CLV_PCSK_SKI1_1	145	149	PF00082	0.543
CLV_PCSK_SKI1_1	161	165	PF00082	0.238
CLV_PCSK_SKI1_1	179	183	PF00082	0.239
CLV_PCSK_SKI1_1	220	224	PF00082	0.608
CLV_PCSK_SKI1_1	351	355	PF00082	0.566
CLV_PCSK_SKI1_1	40	44	PF00082	0.561
CLV_PCSK_SKI1_1	57	61	PF00082	0.570
CLV_PCSK_SKI1_1	93	97	PF00082	0.612
CLV_Separin_Metazoa	90	94	PF03568	0.608
DOC_ANK_TNKS_1	381	388	PF00023	0.561
DOC_ANK_TNKS_1	427	434	PF00023	0.532
DOC_CYCLIN_RxL_1	110	120	PF00134	0.517
DOC_MAPK_gen_1	47	56	PF00069	0.477
DOC_PP1_RVXF_1	432	438	PF00149	0.615
DOC_PP4_FxxP_1	400	403	PF00568	0.589
DOC_USP7_MATH_1	151	155	PF00917	0.603
DOC_USP7_MATH_1	2	6	PF00917	0.561
DOC_USP7_MATH_1	268	272	PF00917	0.351
DOC_USP7_MATH_1	356	360	PF00917	0.649
DOC_USP7_MATH_1	79	83	PF00917	0.643
DOC_USP7_MATH_1	9	13	PF00917	0.585
DOC_USP7_UBL2_3	110	114	PF12436	0.652
DOC_USP7_UBL2_3	125	129	PF12436	0.466
DOC_USP7_UBL2_3	141	145	PF12436	0.518
DOC_USP7_UBL2_3	219	223	PF12436	0.515
DOC_USP7_UBL2_3	23	27	PF12436	0.638
DOC_USP7_UBL2_3	354	358	PF12436	0.543
DOC_USP7_UBL2_3	434	438	PF12436	0.629
LIG_14-3-3_CanoR_1	80	88	PF00244	0.701
LIG_BIR_II_1	1	5	PF00653	0.554
LIG_BIR_III_4	103	107	PF00653	0.553
LIG_deltaCOP1_diTrp_1	283	290	PF00928	0.431
LIG_FHA_1	122	128	PF00498	0.490
LIG_FHA_1	160	166	PF00498	0.274
LIG_FHA_1	225	231	PF00498	0.424
LIG_FHA_1	256	262	PF00498	0.585
LIG_FHA_2	118	124	PF00498	0.607
LIG_FHA_2	255	261	PF00498	0.365
LIG_FHA_2	279	285	PF00498	0.444
LIG_FHA_2	330	336	PF00498	0.478
LIG_FHA_2	403	409	PF00498	0.501
LIG_FHA_2	41	47	PF00498	0.469
LIG_Integrin_isoDGR_2	338	340	PF01839	0.244
LIG_Integrin_RGD_1	333	335	PF01839	0.278
LIG_LIR_Apic_2	398	403	PF02991	0.588
LIG_LIR_Gen_1	116	122	PF02991	0.558
LIG_LIR_Gen_1	227	235	PF02991	0.392
LIG_LIR_Gen_1	284	295	PF02991	0.452
LIG_LIR_Gen_1	394	403	PF02991	0.707
LIG_LIR_Nem_3	116	121	PF02991	0.483
LIG_LIR_Nem_3	21	25	PF02991	0.652
LIG_LIR_Nem_3	227	232	PF02991	0.376
LIG_LIR_Nem_3	284	290	PF02991	0.452
LIG_LIR_Nem_3	394	400	PF02991	0.709
LIG_LIR_Nem_3	411	415	PF02991	0.490
LIG_Pex14_2	118	122	PF04695	0.648
LIG_PTB_Apo_2	273	280	PF02174	0.431
LIG_PTB_Phospho_1	273	279	PF10480	0.431
LIG_SH2_GRB2like	397	400	PF00017	0.551
LIG_SH2_STAP1	224	228	PF00017	0.319
LIG_SH2_STAT5	229	232	PF00017	0.355
LIG_SH2_STAT5	317	320	PF00017	0.431
LIG_SH3_2	442	447	PF14604	0.588
LIG_SH3_3	436	442	PF00018	0.571
LIG_SH3_4	23	30	PF00018	0.693
LIG_TRAF2_1	132	135	PF00917	0.599
LIG_TRAF2_1	154	157	PF00917	0.581
LIG_TRAF2_1	196	199	PF00917	0.470
LIG_TRAF2_1	281	284	PF00917	0.431
LIG_UBA3_1	50	57	PF00899	0.670
LIG_WRC_WIRS_1	286	291	PF05994	0.440
MOD_CK1_1	12	18	PF00069	0.457
MOD_CK1_1	183	189	PF00069	0.548
MOD_CK1_1	234	240	PF00069	0.495
MOD_CK1_1	391	397	PF00069	0.598
MOD_CK1_1	4	10	PF00069	0.588
MOD_CK1_1	82	88	PF00069	0.775
MOD_CK1_1	98	104	PF00069	0.577
MOD_CK2_1	113	119	PF00069	0.569
MOD_CK2_1	151	157	PF00069	0.587
MOD_CK2_1	237	243	PF00069	0.485
MOD_CK2_1	278	284	PF00069	0.431
MOD_CK2_1	356	362	PF00069	0.528
MOD_CK2_1	40	46	PF00069	0.479
MOD_Cter_Amidation	73	76	PF01082	0.806
MOD_GlcNHglycan	102	107	PF01048	0.529
MOD_GlcNHglycan	224	227	PF01048	0.313
MOD_GlcNHglycan	233	236	PF01048	0.389
MOD_GlcNHglycan	239	242	PF01048	0.385
MOD_GlcNHglycan	382	385	PF01048	0.783
MOD_GlcNHglycan	393	396	PF01048	0.538
MOD_GlcNHglycan	65	70	PF01048	0.671
MOD_GlcNHglycan	7	10	PF01048	0.559
MOD_GlcNHglycan	83	87	PF01048	0.708
MOD_GlcNHglycan	93	96	PF01048	0.725
MOD_GSK3_1	1	8	PF00069	0.528
MOD_GSK3_1	113	120	PF00069	0.571
MOD_GSK3_1	136	143	PF00069	0.686
MOD_GSK3_1	391	398	PF00069	0.694
MOD_GSK3_1	404	411	PF00069	0.533
MOD_GSK3_1	91	98	PF00069	0.674
MOD_N-GLC_1	279	284	PF02516	0.240
MOD_N-GLC_1	347	352	PF02516	0.601
MOD_NEK2_1	1	6	PF00069	0.615
MOD_NEK2_1	117	122	PF00069	0.650
MOD_NEK2_1	231	236	PF00069	0.413
MOD_NEK2_1	25	30	PF00069	0.656
MOD_NEK2_1	32	37	PF00069	0.690
MOD_NEK2_1	321	326	PF00069	0.496
MOD_NEK2_2	268	273	PF00069	0.351
MOD_PKA_1	113	119	PF00069	0.472
MOD_PKA_1	140	146	PF00069	0.642
MOD_PKA_1	363	369	PF00069	0.514
MOD_PKA_1	40	46	PF00069	0.491
MOD_PKA_1	447	453	PF00069	0.529
MOD_PKA_2	136	142	PF00069	0.560
MOD_PKA_2	306	312	PF00069	0.431
MOD_PKA_2	363	369	PF00069	0.507
MOD_PKA_2	391	397	PF00069	0.558
MOD_PKA_2	447	453	PF00069	0.529
MOD_PKA_2	79	85	PF00069	0.744
MOD_PKB_1	138	146	PF00069	0.636
MOD_Plk_1	268	274	PF00069	0.353
MOD_Plk_4	113	119	PF00069	0.548
MOD_Plk_4	268	274	PF00069	0.352
MOD_SUMO_rev_2	119	127	PF00179	0.603
MOD_SUMO_rev_2	217	225	PF00179	0.501
MOD_SUMO_rev_2	292	301	PF00179	0.487
MOD_SUMO_rev_2	306	315	PF00179	0.487
MOD_SUMO_rev_2	350	356	PF00179	0.652
MOD_SUMO_rev_2	426	435	PF00179	0.552
MOD_SUMO_rev_2	53	59	PF00179	0.560
TRG_DiLeu_BaEn_1	243	248	PF01217	0.430
TRG_DiLeu_BaEn_2	268	274	PF01217	0.365
TRG_ENDOCYTIC_2	229	232	PF00928	0.375
TRG_ENDOCYTIC_2	317	320	PF00928	0.431
TRG_ENDOCYTIC_2	397	400	PF00928	0.591
TRG_ER_diArg_1	137	140	PF00400	0.618
TRG_ER_diArg_1	190	193	PF00400	0.454
TRG_ER_diArg_1	300	303	PF00400	0.434
TRG_ER_diArg_1	419	421	PF00400	0.609
TRG_NLS_Bipartite_1	363	383	PF00514	0.648
TRG_NLS_Bipartite_1	40	61	PF00514	0.469
TRG_NLS_MonoCore_2	400	405	PF00514	0.680
TRG_NLS_MonoExtC_3	139	144	PF00514	0.511
TRG_NLS_MonoExtC_3	400	406	PF00514	0.594
TRG_NLS_MonoExtC_3	446	451	PF00514	0.558
TRG_NLS_MonoExtC_3	56	61	PF00514	0.450
TRG_NLS_MonoExtN_4	137	144	PF00514	0.571
TRG_NLS_MonoExtN_4	445	452	PF00514	0.531
TRG_Pf-PMV_PEXEL_1	115	119	PF00026	0.564
TRG_Pf-PMV_PEXEL_1	212	217	PF00026	0.405
TRG_Pf-PMV_PEXEL_1	428	432	PF00026	0.530
TRG_Pf-PMV_PEXEL_1	49	53	PF00026	0.531

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8R8	Leptomonas seymouri	72%	100%
A0A1X0P6L1	Trypanosomatidae	46%	100%
A0A3Q8IPK7	Leishmania donovani	93%	100%
A0A3R7LUT6	Trypanosoma rangeli	47%	100%
A4HMS6	Leishmania braziliensis	85%	99%
C9ZZA1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	100%
E9B6D4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5BNV3	Trypanosoma cruzi	47%	100%