Putative phosphoinositide-binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Autophagy-related protein 24

Species:

Leishmania major

UniProt:

E9AF66_LEIMA

TriTrypDb:

LmjF.35.2420 , LMJLV39_350031000 , LMJSD75_350030500

Length:

417

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	yes	yes: 4
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005768	endosome	7	2
GO:0012506	vesicle membrane	4	2
GO:0016020	membrane	2	2
GO:0030659	cytoplasmic vesicle membrane	5	2
GO:0030666	endocytic vesicle membrane	5	2
GO:0031090	organelle membrane	3	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AF66

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF66

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006897	endocytosis	5	2
GO:0006898	receptor-mediated endocytosis	6	2
GO:0006914	autophagy	3	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009987	cellular process	1	2
GO:0016192	vesicle-mediated transport	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0061919	process utilizing autophagic mechanism	2	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005543	phospholipid binding	3	12
GO:0008289	lipid binding	2	12
GO:0035091	phosphatidylinositol binding	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	105	107	PF00675	0.365
CLV_NRD_NRD_1	110	112	PF00675	0.335
CLV_NRD_NRD_1	236	238	PF00675	0.329
CLV_NRD_NRD_1	272	274	PF00675	0.450
CLV_NRD_NRD_1	47	49	PF00675	0.334
CLV_PCSK_FUR_1	234	238	PF00082	0.448
CLV_PCSK_KEX2_1	105	107	PF00082	0.448
CLV_PCSK_KEX2_1	110	112	PF00082	0.448
CLV_PCSK_KEX2_1	236	238	PF00082	0.334
CLV_PCSK_KEX2_1	47	49	PF00082	0.321
CLV_PCSK_PC7_1	106	112	PF00082	0.448
CLV_PCSK_SKI1_1	110	114	PF00082	0.328
CLV_PCSK_SKI1_1	225	229	PF00082	0.482
CLV_PCSK_SKI1_1	384	388	PF00082	0.316
CLV_PCSK_SKI1_1	409	413	PF00082	0.593
DEG_APCC_DBOX_1	109	117	PF00400	0.331
DEG_APCC_DBOX_1	224	232	PF00400	0.351
DEG_APCC_DBOX_1	304	312	PF00400	0.331
DEG_APCC_DBOX_1	408	416	PF00400	0.592
DEG_SPOP_SBC_1	164	168	PF00917	0.710
DOC_CYCLIN_yClb5_NLxxxL_5	225	231	PF00134	0.452
DOC_MAPK_gen_1	388	395	PF00069	0.448
DOC_MAPK_MEF2A_6	388	397	PF00069	0.448
DOC_SPAK_OSR1_1	149	153	PF12202	0.525
DOC_USP7_MATH_1	165	169	PF00917	0.718
DOC_USP7_MATH_1	174	178	PF00917	0.728
DOC_USP7_MATH_1	192	196	PF00917	0.438
DOC_USP7_MATH_1	3	7	PF00917	0.582
DOC_USP7_UBL2_3	384	388	PF12436	0.316
LIG_14-3-3_CanoR_1	105	110	PF00244	0.414
LIG_14-3-3_CanoR_1	149	159	PF00244	0.600
LIG_14-3-3_CanoR_1	208	217	PF00244	0.500
LIG_14-3-3_CanoR_1	236	245	PF00244	0.367
LIG_14-3-3_CanoR_1	289	295	PF00244	0.438
LIG_14-3-3_CanoR_1	334	343	PF00244	0.438
LIG_14-3-3_CanoR_1	47	55	PF00244	0.427
LIG_APCC_ABBA_1	372	377	PF00400	0.425
LIG_BIR_II_1	1	5	PF00653	0.589
LIG_BRCT_BRCA1_1	368	372	PF00533	0.452
LIG_FHA_1	286	292	PF00498	0.375
LIG_FHA_1	31	37	PF00498	0.428
LIG_FHA_2	365	371	PF00498	0.418
LIG_FHA_2	48	54	PF00498	0.452
LIG_FHA_2	93	99	PF00498	0.452
LIG_LIR_Apic_2	26	31	PF02991	0.501
LIG_LIR_Gen_1	369	377	PF02991	0.450
LIG_LIR_Gen_1	404	412	PF02991	0.436
LIG_LIR_Gen_1	50	59	PF02991	0.316
LIG_LIR_Gen_1	62	72	PF02991	0.314
LIG_LIR_Nem_3	103	107	PF02991	0.448
LIG_LIR_Nem_3	24	30	PF02991	0.491
LIG_LIR_Nem_3	248	252	PF02991	0.406
LIG_LIR_Nem_3	369	375	PF02991	0.455
LIG_LIR_Nem_3	404	408	PF02991	0.327
LIG_LIR_Nem_3	50	55	PF02991	0.316
LIG_LIR_Nem_3	62	68	PF02991	0.314
LIG_Pex14_2	245	249	PF04695	0.316
LIG_Pex14_2	368	372	PF04695	0.443
LIG_SH2_CRK	104	108	PF00017	0.448
LIG_SH2_CRK	118	122	PF00017	0.448
LIG_SH2_CRK	65	69	PF00017	0.375
LIG_SH2_PTP2	28	31	PF00017	0.501
LIG_SH2_SRC	28	31	PF00017	0.496
LIG_SH2_STAP1	279	283	PF00017	0.406
LIG_SH2_STAT5	213	216	PF00017	0.325
LIG_SH2_STAT5	28	31	PF00017	0.496
LIG_SH2_STAT5	297	300	PF00017	0.375
LIG_SH2_STAT5	317	320	PF00017	0.155
LIG_SH2_STAT5	49	52	PF00017	0.468
LIG_SH2_STAT5	65	68	PF00017	0.244
LIG_SH3_2	144	149	PF14604	0.585
LIG_SH3_2	69	74	PF14604	0.316
LIG_SH3_3	141	147	PF00018	0.593
LIG_SH3_3	66	72	PF00018	0.322
LIG_SH3_3	8	14	PF00018	0.411
LIG_SUMO_SIM_par_1	391	396	PF11976	0.448
LIG_TRAF2_1	155	158	PF00917	0.647
LIG_TYR_ITIM	102	107	PF00017	0.448
LIG_TYR_ITIM	116	121	PF00017	0.448
LIG_WW_3	146	150	PF00397	0.575
MOD_CK1_1	167	173	PF00069	0.755
MOD_CK1_1	195	201	PF00069	0.540
MOD_CK1_1	240	246	PF00069	0.473
MOD_CK1_1	292	298	PF00069	0.438
MOD_CK2_1	151	157	PF00069	0.570
MOD_CK2_1	364	370	PF00069	0.441
MOD_CK2_1	393	399	PF00069	0.448
MOD_CK2_1	47	53	PF00069	0.334
MOD_CK2_1	87	93	PF00069	0.433
MOD_Cter_Amidation	271	274	PF01082	0.448
MOD_GlcNHglycan	167	170	PF01048	0.708
MOD_GlcNHglycan	176	179	PF01048	0.606
MOD_GlcNHglycan	194	197	PF01048	0.484
MOD_GlcNHglycan	239	242	PF01048	0.448
MOD_GlcNHglycan	312	315	PF01048	0.365
MOD_GlcNHglycan	330	333	PF01048	0.363
MOD_GlcNHglycan	336	339	PF01048	0.490
MOD_GlcNHglycan	89	92	PF01048	0.345
MOD_GSK3_1	159	166	PF00069	0.621
MOD_GSK3_1	19	26	PF00069	0.544
MOD_GSK3_1	203	210	PF00069	0.546
MOD_GSK3_1	285	292	PF00069	0.423
MOD_GSK3_1	393	400	PF00069	0.452
MOD_GSK3_1	47	54	PF00069	0.434
MOD_GSK3_1	87	94	PF00069	0.333
MOD_LATS_1	235	241	PF00433	0.452
MOD_N-GLC_1	284	289	PF02516	0.423
MOD_NEK2_1	150	155	PF00069	0.527
MOD_NEK2_1	23	28	PF00069	0.540
MOD_NEK2_1	245	250	PF00069	0.399
MOD_NEK2_1	290	295	PF00069	0.432
MOD_NEK2_1	328	333	PF00069	0.495
MOD_NEK2_1	393	398	PF00069	0.416
MOD_NEK2_1	411	416	PF00069	0.598
MOD_NEK2_1	59	64	PF00069	0.452
MOD_NEK2_1	87	92	PF00069	0.393
MOD_PIKK_1	12	18	PF00454	0.570
MOD_PIKK_1	184	190	PF00454	0.651
MOD_PIKK_1	195	201	PF00454	0.484
MOD_PKA_1	105	111	PF00069	0.448
MOD_PKA_1	47	53	PF00069	0.331
MOD_PKA_2	105	111	PF00069	0.448
MOD_PKA_2	207	213	PF00069	0.438
MOD_PKA_2	304	310	PF00069	0.316
MOD_PKA_2	47	53	PF00069	0.390
MOD_Plk_1	203	209	PF00069	0.483
MOD_Plk_1	23	29	PF00069	0.518
MOD_Plk_1	285	291	PF00069	0.448
MOD_Plk_1	364	370	PF00069	0.448
MOD_Plk_1	393	399	PF00069	0.449
MOD_Plk_2-3	73	79	PF00069	0.448
MOD_Plk_4	23	29	PF00069	0.564
MOD_Plk_4	292	298	PF00069	0.448
MOD_Plk_4	59	65	PF00069	0.452
MOD_SUMO_for_1	401	404	PF00179	0.448
MOD_SUMO_rev_2	377	386	PF00179	0.328
TRG_DiLeu_BaEn_1	286	291	PF01217	0.448
TRG_DiLeu_BaEn_4	256	262	PF01217	0.448
TRG_ENDOCYTIC_2	104	107	PF00928	0.448
TRG_ENDOCYTIC_2	118	121	PF00928	0.448
TRG_ENDOCYTIC_2	297	300	PF00928	0.316
TRG_ENDOCYTIC_2	49	52	PF00928	0.316
TRG_ENDOCYTIC_2	65	68	PF00928	0.316
TRG_ER_diArg_1	104	106	PF00400	0.316
TRG_ER_diArg_1	109	111	PF00400	0.316
TRG_ER_diArg_1	234	237	PF00400	0.342
TRG_ER_diArg_1	47	49	PF00400	0.445
TRG_NES_CRM1_1	385	399	PF08389	0.448
TRG_Pf-PMV_PEXEL_1	225	229	PF00026	0.405

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IBS5	Leptomonas seymouri	76%	98%
A0A0S4KLA7	Bodo saltans	34%	100%
A0A1X0P5K9	Trypanosomatidae	34%	100%
A0A3Q8IV97	Leishmania donovani	97%	100%
A0A3R7L2W7	Trypanosoma rangeli	34%	99%
A4HMR6	Leishmania braziliensis	93%	100%
A4IBF2	Leishmania infantum	97%	100%
C9ZZB2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9B6C2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O60749	Homo sapiens	24%	80%
P0C220	Macaca fascicularis	23%	80%
Q2TBW7	Bos taurus	24%	80%
Q5R9A9	Pongo abelii	23%	80%
Q9CWK8	Mus musculus	24%	80%
V5B3T2	Trypanosoma cruzi	34%	99%