Putative ubiquitin hydrolase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin hydrolase, putative

Species:

Leishmania major

UniProt:

E9AF65_LEIMA

TriTrypDb:

LmjF.35.2410 , LMJLV39_350030900 , LMJSD75_350030400

Length:

923

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 4
NetGPI	no	yes: 0, no: 4

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005829	cytosol	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AF65

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF65

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	5
GO:0006511	ubiquitin-dependent protein catabolic process	7	5
GO:0006807	nitrogen compound metabolic process	2	5
GO:0008152	metabolic process	1	5
GO:0009056	catabolic process	2	5
GO:0009057	macromolecule catabolic process	4	5
GO:0009987	cellular process	1	5
GO:0016579	protein deubiquitination	6	5
GO:0019538	protein metabolic process	3	5
GO:0019941	modification-dependent protein catabolic process	6	5
GO:0036211	protein modification process	4	5
GO:0043170	macromolecule metabolic process	3	5
GO:0043412	macromolecule modification	4	5
GO:0043632	modification-dependent macromolecule catabolic process	5	5
GO:0044237	cellular metabolic process	2	5
GO:0044238	primary metabolic process	2	5
GO:0044248	cellular catabolic process	3	5
GO:0044260	obsolete cellular macromolecule metabolic process	3	5
GO:0044265	obsolete cellular macromolecule catabolic process	4	5
GO:0051603	proteolysis involved in protein catabolic process	5	5
GO:0070646	protein modification by small protein removal	5	5
GO:0070647	protein modification by small protein conjugation or removal	5	5
GO:0071704	organic substance metabolic process	2	5
GO:1901564	organonitrogen compound metabolic process	3	5
GO:1901575	organic substance catabolic process	3	5

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	5
GO:0004175	endopeptidase activity	4	2
GO:0004197	cysteine-type endopeptidase activity	5	2
GO:0004843	cysteine-type deubiquitinase activity	5	5
GO:0008233	peptidase activity	3	5
GO:0008234	cysteine-type peptidase activity	4	5
GO:0016787	hydrolase activity	2	5
GO:0019783	ubiquitin-like protein peptidase activity	4	5
GO:0101005	deubiquitinase activity	5	5
GO:0140096	catalytic activity, acting on a protein	2	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	46	50	PF00656	0.742
CLV_C14_Caspase3-7	667	671	PF00656	0.316
CLV_C14_Caspase3-7	916	920	PF00656	0.518
CLV_NRD_NRD_1	194	196	PF00675	0.831
CLV_NRD_NRD_1	25	27	PF00675	0.750
CLV_NRD_NRD_1	42	44	PF00675	0.636
CLV_NRD_NRD_1	481	483	PF00675	0.356
CLV_NRD_NRD_1	499	501	PF00675	0.332
CLV_NRD_NRD_1	517	519	PF00675	0.304
CLV_NRD_NRD_1	72	74	PF00675	0.699
CLV_NRD_NRD_1	795	797	PF00675	0.700
CLV_NRD_NRD_1	829	831	PF00675	0.659
CLV_NRD_NRD_1	913	915	PF00675	0.573
CLV_PCSK_FUR_1	23	27	PF00082	0.628
CLV_PCSK_FUR_1	716	720	PF00082	0.386
CLV_PCSK_KEX2_1	194	196	PF00082	0.831
CLV_PCSK_KEX2_1	23	25	PF00082	0.739
CLV_PCSK_KEX2_1	498	500	PF00082	0.477
CLV_PCSK_KEX2_1	718	720	PF00082	0.426
CLV_PCSK_KEX2_1	736	738	PF00082	0.501
CLV_PCSK_KEX2_1	829	831	PF00082	0.659
CLV_PCSK_KEX2_1	861	863	PF00082	0.702
CLV_PCSK_KEX2_1	913	915	PF00082	0.584
CLV_PCSK_PC1ET2_1	718	720	PF00082	0.386
CLV_PCSK_PC1ET2_1	736	738	PF00082	0.501
CLV_PCSK_PC1ET2_1	861	863	PF00082	0.639
CLV_PCSK_SKI1_1	168	172	PF00082	0.644
CLV_PCSK_SKI1_1	235	239	PF00082	0.616
CLV_PCSK_SKI1_1	343	347	PF00082	0.658
CLV_PCSK_SKI1_1	425	429	PF00082	0.664
CLV_PCSK_SKI1_1	690	694	PF00082	0.412
CLV_PCSK_SKI1_1	759	763	PF00082	0.377
CLV_PCSK_SKI1_1	877	881	PF00082	0.663
CLV_PCSK_SKI1_1	890	894	PF00082	0.370
CLV_Separin_Metazoa	687	691	PF03568	0.526
DEG_COP1_1	437	445	PF00400	0.598
DEG_SPOP_SBC_1	202	206	PF00917	0.675
DEG_SPOP_SBC_1	521	525	PF00917	0.526
DOC_CDC14_PxL_1	345	353	PF14671	0.711
DOC_CKS1_1	475	480	PF01111	0.412
DOC_CYCLIN_RxL_1	230	241	PF00134	0.613
DOC_CYCLIN_RxL_1	422	432	PF00134	0.631
DOC_CYCLIN_yCln2_LP_2	701	707	PF00134	0.388
DOC_MAPK_MEF2A_6	642	651	PF00069	0.526
DOC_MAPK_RevD_3	898	914	PF00069	0.554
DOC_PP1_RVXF_1	604	611	PF00149	0.433
DOC_PP2B_LxvP_1	346	349	PF13499	0.599
DOC_SPAK_OSR1_1	642	646	PF12202	0.433
DOC_USP7_MATH_1	137	141	PF00917	0.658
DOC_USP7_MATH_1	298	302	PF00917	0.680
DOC_USP7_MATH_1	372	376	PF00917	0.708
DOC_USP7_MATH_1	416	420	PF00917	0.688
DOC_USP7_MATH_1	52	56	PF00917	0.775
DOC_USP7_MATH_1	521	525	PF00917	0.526
DOC_USP7_MATH_1	851	855	PF00917	0.687
DOC_USP7_UBL2_3	156	160	PF12436	0.625
DOC_USP7_UBL2_3	672	676	PF12436	0.396
DOC_WW_Pin1_4	248	253	PF00397	0.742
DOC_WW_Pin1_4	267	272	PF00397	0.575
DOC_WW_Pin1_4	303	308	PF00397	0.728
DOC_WW_Pin1_4	355	360	PF00397	0.742
DOC_WW_Pin1_4	362	367	PF00397	0.687
DOC_WW_Pin1_4	474	479	PF00397	0.412
DOC_WW_Pin1_4	58	63	PF00397	0.565
DOC_WW_Pin1_4	675	680	PF00397	0.316
DOC_WW_Pin1_4	81	86	PF00397	0.779
DOC_WW_Pin1_4	820	825	PF00397	0.814
DOC_WW_Pin1_4	856	861	PF00397	0.599
DOC_WW_Pin1_4	97	102	PF00397	0.664
LIG_14-3-3_CanoR_1	138	143	PF00244	0.616
LIG_14-3-3_CanoR_1	233	238	PF00244	0.612
LIG_14-3-3_CanoR_1	35	41	PF00244	0.690
LIG_14-3-3_CanoR_1	498	507	PF00244	0.344
LIG_14-3-3_CanoR_1	606	611	PF00244	0.433
LIG_14-3-3_CanoR_1	719	725	PF00244	0.386
LIG_14-3-3_CanoR_1	772	780	PF00244	0.526
LIG_14-3-3_CanoR_1	852	860	PF00244	0.691
LIG_Actin_WH2_2	801	816	PF00022	0.562
LIG_BIR_II_1	1	5	PF00653	0.758
LIG_BRCT_BRCA1_1	639	643	PF00533	0.526
LIG_eIF4E_1	641	647	PF01652	0.433
LIG_FHA_1	134	140	PF00498	0.588
LIG_FHA_1	386	392	PF00498	0.680
LIG_FHA_1	462	468	PF00498	0.475
LIG_FHA_1	475	481	PF00498	0.454
LIG_FHA_1	5	11	PF00498	0.692
LIG_FHA_1	521	527	PF00498	0.532
LIG_FHA_1	745	751	PF00498	0.334
LIG_FHA_2	107	113	PF00498	0.749
LIG_FHA_2	139	145	PF00498	0.688
LIG_FHA_2	379	385	PF00498	0.705
LIG_FHA_2	589	595	PF00498	0.489
LIG_FHA_2	616	622	PF00498	0.437
LIG_FHA_2	662	668	PF00498	0.349
LIG_FHA_2	682	688	PF00498	0.260
LIG_FHA_2	814	820	PF00498	0.650
LIG_FHA_2	894	900	PF00498	0.455
LIG_HP1_1	729	733	PF01393	0.316
LIG_Integrin_isoDGR_2	863	865	PF01839	0.742
LIG_IRF3_LxIS_1	643	650	PF10401	0.526
LIG_LIR_Apic_2	174	179	PF02991	0.601
LIG_LIR_Apic_2	723	729	PF02991	0.433
LIG_LIR_Gen_1	358	367	PF02991	0.632
LIG_LIR_Gen_1	617	626	PF02991	0.456
LIG_LIR_Gen_1	640	651	PF02991	0.433
LIG_LIR_Gen_1	781	788	PF02991	0.373
LIG_LIR_Gen_1	906	915	PF02991	0.540
LIG_LIR_Nem_3	172	176	PF02991	0.726
LIG_LIR_Nem_3	358	364	PF02991	0.630
LIG_LIR_Nem_3	590	595	PF02991	0.526
LIG_LIR_Nem_3	617	623	PF02991	0.437
LIG_LIR_Nem_3	640	646	PF02991	0.433
LIG_LIR_Nem_3	725	730	PF02991	0.383
LIG_LIR_Nem_3	777	783	PF02991	0.456
LIG_LIR_Nem_3	806	812	PF02991	0.499
LIG_LIR_Nem_3	906	911	PF02991	0.526
LIG_NRBOX	569	575	PF00104	0.526
LIG_PCNA_yPIPBox_3	468	482	PF02747	0.480
LIG_PDZ_Class_1	918	923	PF00595	0.617
LIG_Pex14_2	751	755	PF04695	0.526
LIG_REV1ctd_RIR_1	691	701	PF16727	0.526
LIG_SH2_CRK	783	787	PF00017	0.480
LIG_SH2_CRK	809	813	PF00017	0.509
LIG_SH2_GRB2like	466	469	PF00017	0.526
LIG_SH2_SRC	466	469	PF00017	0.526
LIG_SH2_STAP1	240	244	PF00017	0.692
LIG_SH2_STAP1	604	608	PF00017	0.526
LIG_SH2_STAP1	722	726	PF00017	0.433
LIG_SH2_STAT5	466	469	PF00017	0.383
LIG_SH2_STAT5	753	756	PF00017	0.526
LIG_SH2_STAT5	780	783	PF00017	0.393
LIG_SH2_STAT5	785	788	PF00017	0.343
LIG_SH2_STAT5	885	888	PF00017	0.560
LIG_SH2_STAT5	908	911	PF00017	0.524
LIG_SH3_1	406	412	PF00018	0.698
LIG_SH3_3	178	184	PF00018	0.652
LIG_SH3_3	304	310	PF00018	0.659
LIG_SH3_3	406	412	PF00018	0.744
LIG_SH3_3	45	51	PF00018	0.667
LIG_SH3_3	56	62	PF00018	0.594
LIG_SH3_3	746	752	PF00018	0.526
LIG_SH3_4	585	592	PF00018	0.386
LIG_SUMO_SIM_anti_2	896	902	PF11976	0.400
LIG_SUMO_SIM_par_1	523	531	PF11976	0.526
LIG_TRAF2_1	207	210	PF00917	0.654
LIG_TYR_ITIM	910	915	PF00017	0.550
LIG_TYR_ITSM	779	786	PF00017	0.526
LIG_UBA3_1	543	548	PF00899	0.501
LIG_UBA3_1	570	579	PF00899	0.526
LIG_WRC_WIRS_1	391	396	PF05994	0.729
LIG_WW_2	307	310	PF00397	0.606
LIG_WW_3	276	280	PF00397	0.606
MOD_CDC14_SPxK_1	859	862	PF00782	0.628
MOD_CDK_SPK_2	675	680	PF00069	0.316
MOD_CDK_SPK_2	81	86	PF00069	0.686
MOD_CDK_SPK_2	856	861	PF00069	0.623
MOD_CDK_SPxK_1	856	862	PF00069	0.633
MOD_CDK_SPxxK_3	267	274	PF00069	0.629
MOD_CK1_1	133	139	PF00069	0.621
MOD_CK1_1	152	158	PF00069	0.695
MOD_CK1_1	2	8	PF00069	0.817
MOD_CK1_1	201	207	PF00069	0.742
MOD_CK1_1	262	268	PF00069	0.814
MOD_CK1_1	29	35	PF00069	0.609
MOD_CK1_1	316	322	PF00069	0.685
MOD_CK1_1	325	331	PF00069	0.674
MOD_CK1_1	370	376	PF00069	0.752
MOD_CK1_1	387	393	PF00069	0.749
MOD_CK1_1	399	405	PF00069	0.676
MOD_CK1_1	426	432	PF00069	0.689
MOD_CK1_1	434	440	PF00069	0.688
MOD_CK1_1	444	450	PF00069	0.450
MOD_CK1_1	654	660	PF00069	0.433
MOD_CK1_1	823	829	PF00069	0.681
MOD_CK1_1	9	15	PF00069	0.705
MOD_CK1_1	97	103	PF00069	0.709
MOD_CK2_1	106	112	PF00069	0.749
MOD_CK2_1	203	209	PF00069	0.618
MOD_CK2_1	378	384	PF00069	0.705
MOD_CK2_1	511	517	PF00069	0.526
MOD_CK2_1	661	667	PF00069	0.392
MOD_CK2_1	681	687	PF00069	0.260
MOD_CMANNOS	607	610	PF00535	0.433
MOD_Cter_Amidation	496	499	PF01082	0.419
MOD_GlcNHglycan	151	154	PF01048	0.739
MOD_GlcNHglycan	162	165	PF01048	0.623
MOD_GlcNHglycan	198	201	PF01048	0.710
MOD_GlcNHglycan	205	208	PF01048	0.639
MOD_GlcNHglycan	254	257	PF01048	0.738
MOD_GlcNHglycan	327	330	PF01048	0.818
MOD_GlcNHglycan	36	39	PF01048	0.779
MOD_GlcNHglycan	416	419	PF01048	0.715
MOD_GlcNHglycan	433	436	PF01048	0.778
MOD_GlcNHglycan	443	446	PF01048	0.548
MOD_GlcNHglycan	45	48	PF01048	0.718
MOD_GlcNHglycan	70	73	PF01048	0.763
MOD_GlcNHglycan	776	779	PF01048	0.512
MOD_GlcNHglycan	873	876	PF01048	0.728
MOD_GSK3_1	1	8	PF00069	0.756
MOD_GSK3_1	112	119	PF00069	0.730
MOD_GSK3_1	128	135	PF00069	0.789
MOD_GSK3_1	198	205	PF00069	0.671
MOD_GSK3_1	229	236	PF00069	0.674
MOD_GSK3_1	243	250	PF00069	0.682
MOD_GSK3_1	26	33	PF00069	0.747
MOD_GSK3_1	262	269	PF00069	0.725
MOD_GSK3_1	309	316	PF00069	0.633
MOD_GSK3_1	378	385	PF00069	0.699
MOD_GSK3_1	39	46	PF00069	0.712
MOD_GSK3_1	392	399	PF00069	0.758
MOD_GSK3_1	416	423	PF00069	0.693
MOD_GSK3_1	427	434	PF00069	0.667
MOD_GSK3_1	437	444	PF00069	0.616
MOD_GSK3_1	507	514	PF00069	0.377
MOD_GSK3_1	627	634	PF00069	0.399
MOD_GSK3_1	647	654	PF00069	0.321
MOD_GSK3_1	76	83	PF00069	0.671
MOD_GSK3_1	774	781	PF00069	0.433
MOD_GSK3_1	852	859	PF00069	0.711
MOD_GSK3_1	871	878	PF00069	0.780
MOD_N-GLC_1	119	124	PF02516	0.673
MOD_N-GLC_1	279	284	PF02516	0.733
MOD_N-GLC_1	354	359	PF02516	0.743
MOD_N-GLC_1	614	619	PF02516	0.524
MOD_N-GLC_1	81	86	PF02516	0.686
MOD_N-GLC_1	890	895	PF02516	0.516
MOD_NEK2_1	1	6	PF00069	0.758
MOD_NEK2_1	111	116	PF00069	0.634
MOD_NEK2_1	238	243	PF00069	0.663
MOD_NEK2_1	285	290	PF00069	0.740
MOD_NEK2_1	427	432	PF00069	0.637
MOD_NEK2_1	461	466	PF00069	0.356
MOD_NEK2_1	543	548	PF00069	0.388
MOD_NEK2_1	569	574	PF00069	0.526
MOD_NEK2_1	647	652	PF00069	0.399
MOD_NEK2_1	813	818	PF00069	0.622
MOD_NEK2_2	6	11	PF00069	0.641
MOD_OFUCOSY	629	636	PF10250	0.433
MOD_OFUCOSY	769	775	PF10250	0.386
MOD_PIKK_1	243	249	PF00454	0.742
MOD_PIKK_1	370	376	PF00454	0.768
MOD_PIKK_1	656	662	PF00454	0.442
MOD_PIKK_1	9	15	PF00454	0.758
MOD_PKA_1	43	49	PF00069	0.708
MOD_PKA_1	498	504	PF00069	0.414
MOD_PKA_2	137	143	PF00069	0.615
MOD_PKA_2	229	235	PF00069	0.690
MOD_PKA_2	313	319	PF00069	0.707
MOD_PKA_2	34	40	PF00069	0.776
MOD_PKA_2	414	420	PF00069	0.642
MOD_PKA_2	498	504	PF00069	0.439
MOD_PKA_2	76	82	PF00069	0.712
MOD_PKA_2	771	777	PF00069	0.526
MOD_PKA_2	851	857	PF00069	0.708
MOD_PKB_1	24	32	PF00069	0.689
MOD_Plk_1	111	117	PF00069	0.711
MOD_Plk_1	143	149	PF00069	0.687
MOD_Plk_1	614	620	PF00069	0.524
MOD_Plk_1	636	642	PF00069	0.433
MOD_Plk_1	744	750	PF00069	0.480
MOD_Plk_1	893	899	PF00069	0.410
MOD_Plk_2-3	169	175	PF00069	0.613
MOD_Plk_4	423	429	PF00069	0.635
MOD_Plk_4	466	472	PF00069	0.480
MOD_Plk_4	522	528	PF00069	0.406
MOD_Plk_4	53	59	PF00069	0.709
MOD_Plk_4	606	612	PF00069	0.484
MOD_Plk_4	615	621	PF00069	0.421
MOD_Plk_4	781	787	PF00069	0.433
MOD_ProDKin_1	248	254	PF00069	0.743
MOD_ProDKin_1	267	273	PF00069	0.576
MOD_ProDKin_1	303	309	PF00069	0.732
MOD_ProDKin_1	355	361	PF00069	0.744
MOD_ProDKin_1	362	368	PF00069	0.687
MOD_ProDKin_1	474	480	PF00069	0.412
MOD_ProDKin_1	58	64	PF00069	0.567
MOD_ProDKin_1	675	681	PF00069	0.316
MOD_ProDKin_1	81	87	PF00069	0.779
MOD_ProDKin_1	820	826	PF00069	0.812
MOD_ProDKin_1	856	862	PF00069	0.599
MOD_ProDKin_1	97	103	PF00069	0.665
MOD_SUMO_for_1	743	746	PF00179	0.526
MOD_SUMO_rev_2	511	521	PF00179	0.422
MOD_SUMO_rev_2	594	601	PF00179	0.433
MOD_SUMO_rev_2	665	673	PF00179	0.462
MOD_SUMO_rev_2	789	794	PF00179	0.659
TRG_DiLeu_BaLyEn_6	185	190	PF01217	0.627
TRG_DiLeu_BaLyEn_6	808	813	PF01217	0.501
TRG_ENDOCYTIC_2	361	364	PF00928	0.606
TRG_ENDOCYTIC_2	620	623	PF00928	0.451
TRG_ENDOCYTIC_2	783	786	PF00928	0.526
TRG_ENDOCYTIC_2	809	812	PF00928	0.503
TRG_ENDOCYTIC_2	908	911	PF00928	0.524
TRG_ENDOCYTIC_2	912	915	PF00928	0.502
TRG_ER_diArg_1	193	195	PF00400	0.821
TRG_ER_diArg_1	23	26	PF00400	0.742
TRG_ER_diArg_1	333	336	PF00400	0.642
TRG_ER_diArg_1	488	491	PF00400	0.433
TRG_ER_diArg_1	498	500	PF00400	0.316
TRG_ER_diArg_1	655	658	PF00400	0.433
TRG_ER_diArg_1	828	830	PF00400	0.660
TRG_ER_diArg_1	912	914	PF00400	0.558
TRG_NLS_MonoExtC_3	795	800	PF00514	0.654
TRG_Pf-PMV_PEXEL_1	403	408	PF00026	0.735
TRG_Pf-PMV_PEXEL_1	482	487	PF00026	0.480

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3S7X9D2	Leishmania donovani	81%	100%
A4HMR5	Leishmania braziliensis	45%	96%
E9B6C1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	67%	100%