Importin N-terminal domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Importin 1

Species:

Leishmania major

UniProt:

E9AF57_LEIMA

TriTrypDb:

LmjF.35.2330 , LMJLV39_350030100 , LMJSD75_350029500

Length:

919

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	3
GO:0005737	cytoplasm	2	12
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9AF57

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF57

Function

Biological processes
Term	Name	Level	Count
GO:0006405	RNA export from nucleus	5	2
GO:0006406	mRNA export from nucleus	6	2
GO:0006606	protein import into nucleus	5	12
GO:0006810	transport	3	12
GO:0006886	intracellular protein transport	4	12
GO:0006913	nucleocytoplasmic transport	5	12
GO:0008104	protein localization	4	12
GO:0009987	cellular process	1	12
GO:0015031	protein transport	4	12
GO:0015931	nucleobase-containing compound transport	5	2
GO:0033036	macromolecule localization	2	12
GO:0033365	protein localization to organelle	5	12
GO:0034504	protein localization to nucleus	6	12
GO:0045184	establishment of protein localization	3	12
GO:0046907	intracellular transport	3	12
GO:0050657	nucleic acid transport	6	2
GO:0050658	RNA transport	4	2
GO:0051028	mRNA transport	5	2
GO:0051168	nuclear export	6	2
GO:0051169	nuclear transport	4	12
GO:0051170	import into nucleus	6	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0051236	establishment of RNA localization	3	2
GO:0051641	cellular localization	2	12
GO:0051649	establishment of localization in cell	3	12
GO:0070727	cellular macromolecule localization	3	12
GO:0071702	organic substance transport	4	12
GO:0071705	nitrogen compound transport	4	12
GO:0072594	establishment of protein localization to organelle	4	12
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0008152	metabolic process	1	1
GO:0016310	phosphorylation	5	1
GO:0044237	cellular metabolic process	2	1

Molecular functions
Term	Name	Level	Count
GO:0005048	signal sequence binding	4	2
GO:0005488	binding	1	3
GO:0008139	nuclear localization sequence binding	5	2
GO:0033218	amide binding	2	2
GO:0042277	peptide binding	3	2
GO:0061608	nuclear import signal receptor activity	3	2
GO:0140104	molecular carrier activity	1	2
GO:0140142	nucleocytoplasmic carrier activity	2	2
GO:0003824	catalytic activity	1	1
GO:0005515	protein binding	2	1
GO:0016301	kinase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	1
GO:0019899	enzyme binding	3	1
GO:0031267	small GTPase binding	5	1
GO:0051020	GTPase binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	152	156	PF00656	0.367
CLV_C14_Caspase3-7	343	347	PF00656	0.619
CLV_C14_Caspase3-7	458	462	PF00656	0.312
CLV_NRD_NRD_1	384	386	PF00675	0.361
CLV_NRD_NRD_1	496	498	PF00675	0.516
CLV_NRD_NRD_1	908	910	PF00675	0.535
CLV_PCSK_KEX2_1	384	386	PF00082	0.359
CLV_PCSK_SKI1_1	161	165	PF00082	0.392
CLV_PCSK_SKI1_1	295	299	PF00082	0.385
CLV_PCSK_SKI1_1	515	519	PF00082	0.495
CLV_PCSK_SKI1_1	549	553	PF00082	0.342
CLV_PCSK_SKI1_1	883	887	PF00082	0.495
CLV_PCSK_SKI1_1	898	902	PF00082	0.543
DOC_ANK_TNKS_1	357	364	PF00023	0.527
DOC_CDC14_PxL_1	165	173	PF14671	0.495
DOC_CDC14_PxL_1	401	409	PF14671	0.530
DOC_CYCLIN_RxL_1	546	554	PF00134	0.350
DOC_CYCLIN_RxL_1	880	890	PF00134	0.313
DOC_CYCLIN_yCln2_LP_2	159	165	PF00134	0.486
DOC_CYCLIN_yCln2_LP_2	69	75	PF00134	0.474
DOC_MAPK_gen_1	187	196	PF00069	0.433
DOC_MAPK_gen_1	384	391	PF00069	0.359
DOC_MAPK_gen_1	56	63	PF00069	0.454
DOC_MAPK_HePTP_8	186	198	PF00069	0.334
DOC_MAPK_MEF2A_6	189	198	PF00069	0.379
DOC_MAPK_MEF2A_6	242	250	PF00069	0.338
DOC_PP2B_LxvP_1	228	231	PF13499	0.530
DOC_PP2B_LxvP_1	402	405	PF13499	0.403
DOC_PP2B_LxvP_1	577	580	PF13499	0.455
DOC_USP7_MATH_1	13	17	PF00917	0.480
DOC_USP7_MATH_1	513	517	PF00917	0.461
DOC_USP7_MATH_1	555	559	PF00917	0.467
DOC_USP7_MATH_1	638	642	PF00917	0.567
DOC_USP7_MATH_1	664	668	PF00917	0.412
DOC_USP7_MATH_1	732	736	PF00917	0.561
DOC_USP7_MATH_1	738	742	PF00917	0.395
DOC_USP7_MATH_1	767	771	PF00917	0.417
DOC_USP7_MATH_1	814	818	PF00917	0.525
DOC_WW_Pin1_4	14	19	PF00397	0.532
DOC_WW_Pin1_4	585	590	PF00397	0.425
DOC_WW_Pin1_4	689	694	PF00397	0.569
DOC_WW_Pin1_4	763	768	PF00397	0.507
LIG_14-3-3_CanoR_1	141	146	PF00244	0.467
LIG_14-3-3_CanoR_1	384	391	PF00244	0.343
LIG_14-3-3_CanoR_1	535	544	PF00244	0.474
LIG_14-3-3_CanoR_1	623	628	PF00244	0.486
LIG_APCC_ABBA_1	802	807	PF00400	0.401
LIG_APCC_ABBA_1	861	866	PF00400	0.349
LIG_APCC_ABBAyCdc20_2	320	326	PF00400	0.393
LIG_BIR_III_2	690	694	PF00653	0.509
LIG_BIR_III_4	155	159	PF00653	0.488
LIG_BIR_III_4	663	667	PF00653	0.389
LIG_BRCT_BRCA1_1	172	176	PF00533	0.510
LIG_BRCT_BRCA1_1	246	250	PF00533	0.421
LIG_BRCT_BRCA1_1	658	662	PF00533	0.332
LIG_BRCT_BRCA1_1	769	773	PF00533	0.393
LIG_deltaCOP1_diTrp_1	292	300	PF00928	0.370
LIG_eIF4E_1	397	403	PF01652	0.469
LIG_FHA_1	158	164	PF00498	0.405
LIG_FHA_1	216	222	PF00498	0.382
LIG_FHA_1	236	242	PF00498	0.260
LIG_FHA_1	615	621	PF00498	0.458
LIG_FHA_1	651	657	PF00498	0.486
LIG_FHA_2	177	183	PF00498	0.530
LIG_FHA_2	388	394	PF00498	0.402
LIG_FHA_2	586	592	PF00498	0.412
LIG_FHA_2	728	734	PF00498	0.503
LIG_GBD_Chelix_1	118	126	PF00786	0.471
LIG_GBD_Chelix_1	4	12	PF00786	0.420
LIG_LIR_Apic_2	337	342	PF02991	0.453
LIG_LIR_Gen_1	249	259	PF02991	0.392
LIG_LIR_Gen_1	321	332	PF02991	0.421
LIG_LIR_Gen_1	436	447	PF02991	0.415
LIG_LIR_Gen_1	461	471	PF02991	0.414
LIG_LIR_Gen_1	597	607	PF02991	0.397
LIG_LIR_Gen_1	624	632	PF02991	0.456
LIG_LIR_Gen_1	659	669	PF02991	0.419
LIG_LIR_Gen_1	677	688	PF02991	0.498
LIG_LIR_Gen_1	734	744	PF02991	0.436
LIG_LIR_Gen_1	770	780	PF02991	0.473
LIG_LIR_Nem_3	207	213	PF02991	0.395
LIG_LIR_Nem_3	249	254	PF02991	0.392
LIG_LIR_Nem_3	256	262	PF02991	0.378
LIG_LIR_Nem_3	291	297	PF02991	0.373
LIG_LIR_Nem_3	395	401	PF02991	0.481
LIG_LIR_Nem_3	436	442	PF02991	0.490
LIG_LIR_Nem_3	461	467	PF02991	0.422
LIG_LIR_Nem_3	539	544	PF02991	0.496
LIG_LIR_Nem_3	624	628	PF02991	0.370
LIG_LIR_Nem_3	677	683	PF02991	0.517
LIG_LIR_Nem_3	734	740	PF02991	0.402
LIG_LIR_Nem_3	770	776	PF02991	0.471
LIG_NRBOX	452	458	PF00104	0.341
LIG_PCNA_PIPBox_1	769	778	PF02747	0.460
LIG_PCNA_yPIPBox_3	211	221	PF02747	0.408
LIG_Pex14_2	621	625	PF04695	0.501
LIG_Pex14_2	821	825	PF04695	0.326
LIG_PTB_Apo_2	619	626	PF02174	0.499
LIG_Rb_pABgroove_1	593	601	PF01858	0.483
LIG_Rb_pABgroove_1	656	664	PF01858	0.344
LIG_SH2_CRK	210	214	PF00017	0.434
LIG_SH2_CRK	439	443	PF00017	0.475
LIG_SH2_CRK	737	741	PF00017	0.371
LIG_SH2_NCK_1	737	741	PF00017	0.422
LIG_SH2_SRC	251	254	PF00017	0.425
LIG_SH2_SRC	805	808	PF00017	0.432
LIG_SH2_STAP1	439	443	PF00017	0.420
LIG_SH2_STAT3	916	919	PF00017	0.479
LIG_SH2_STAT5	145	148	PF00017	0.478
LIG_SH2_STAT5	170	173	PF00017	0.415
LIG_SH2_STAT5	353	356	PF00017	0.609
LIG_SH2_STAT5	40	43	PF00017	0.468
LIG_SH2_STAT5	480	483	PF00017	0.404
LIG_SH2_STAT5	805	808	PF00017	0.432
LIG_SH3_3	111	117	PF00018	0.359
LIG_SH3_3	86	92	PF00018	0.449
LIG_SUMO_SIM_anti_2	148	155	PF11976	0.350
LIG_SUMO_SIM_anti_2	192	199	PF11976	0.364
LIG_SUMO_SIM_anti_2	400	405	PF11976	0.444
LIG_SUMO_SIM_par_1	315	321	PF11976	0.351
LIG_SUMO_SIM_par_1	465	470	PF11976	0.363
LIG_SUMO_SIM_par_1	549	554	PF11976	0.439
LIG_TRAF2_1	281	284	PF00917	0.502
LIG_TRAF2_1	646	649	PF00917	0.540
LIG_TYR_ITSM	247	254	PF00017	0.483
LIG_UBA3_1	491	498	PF00899	0.446
LIG_UBA3_1	772	778	PF00899	0.451
MOD_CDK_SPxxK_3	689	696	PF00069	0.488
MOD_CK1_1	102	108	PF00069	0.417
MOD_CK1_1	209	215	PF00069	0.421
MOD_CK1_1	3	9	PF00069	0.486
MOD_CK1_1	387	393	PF00069	0.339
MOD_CK1_1	470	476	PF00069	0.386
MOD_CK1_1	536	542	PF00069	0.486
MOD_CK1_1	686	692	PF00069	0.501
MOD_CK1_1	887	893	PF00069	0.506
MOD_CK2_1	322	328	PF00069	0.372
MOD_CK2_1	387	393	PF00069	0.393
MOD_CK2_1	40	46	PF00069	0.374
MOD_CK2_1	405	411	PF00069	0.407
MOD_CK2_1	513	519	PF00069	0.499
MOD_CK2_1	664	670	PF00069	0.408
MOD_CK2_1	805	811	PF00069	0.421
MOD_CK2_1	834	840	PF00069	0.547
MOD_GlcNHglycan	334	337	PF01048	0.495
MOD_GlcNHglycan	386	389	PF01048	0.344
MOD_GlcNHglycan	415	418	PF01048	0.546
MOD_GlcNHglycan	574	577	PF01048	0.473
MOD_GlcNHglycan	640	643	PF01048	0.602
MOD_GlcNHglycan	658	661	PF01048	0.241
MOD_GlcNHglycan	666	669	PF01048	0.401
MOD_GlcNHglycan	81	84	PF01048	0.513
MOD_GlcNHglycan	854	857	PF01048	0.489
MOD_GSK3_1	171	178	PF00069	0.519
MOD_GSK3_1	318	325	PF00069	0.400
MOD_GSK3_1	35	42	PF00069	0.462
MOD_GSK3_1	472	479	PF00069	0.488
MOD_GSK3_1	555	562	PF00069	0.407
MOD_GSK3_1	585	592	PF00069	0.407
MOD_GSK3_1	601	608	PF00069	0.316
MOD_GSK3_1	648	655	PF00069	0.514
MOD_GSK3_1	755	762	PF00069	0.593
MOD_GSK3_1	763	770	PF00069	0.480
MOD_GSK3_1	780	787	PF00069	0.436
MOD_GSK3_1	805	812	PF00069	0.472
MOD_N-GLC_1	35	40	PF02516	0.499
MOD_N-GLC_1	589	594	PF02516	0.447
MOD_N-GLC_1	621	626	PF02516	0.478
MOD_N-GLC_1	727	732	PF02516	0.491
MOD_N-GLC_1	783	788	PF02516	0.585
MOD_N-GLC_1	794	799	PF02516	0.440
MOD_N-GLC_1	887	892	PF02516	0.575
MOD_NEK2_1	171	176	PF00069	0.516
MOD_NEK2_1	244	249	PF00069	0.378
MOD_NEK2_1	35	40	PF00069	0.459
MOD_NEK2_1	413	418	PF00069	0.550
MOD_NEK2_1	465	470	PF00069	0.394
MOD_NEK2_1	594	599	PF00069	0.358
MOD_NEK2_1	621	626	PF00069	0.388
MOD_NEK2_1	65	70	PF00069	0.502
MOD_NEK2_1	650	655	PF00069	0.385
MOD_NEK2_1	681	686	PF00069	0.394
MOD_NEK2_1	780	785	PF00069	0.557
MOD_NEK2_1	867	872	PF00069	0.362
MOD_NEK2_2	732	737	PF00069	0.508
MOD_PIKK_1	230	236	PF00454	0.467
MOD_PIKK_1	3	9	PF00454	0.429
MOD_PIKK_1	392	398	PF00454	0.465
MOD_PIKK_1	47	53	PF00454	0.495
MOD_PIKK_1	583	589	PF00454	0.529
MOD_PIKK_1	767	773	PF00454	0.404
MOD_PK_1	344	350	PF00069	0.614
MOD_PKA_1	384	390	PF00069	0.347
MOD_PKA_2	384	390	PF00069	0.347
MOD_PKA_2	567	573	PF00069	0.507
MOD_PKA_2	832	838	PF00069	0.565
MOD_PKB_1	349	357	PF00069	0.657
MOD_Plk_1	282	288	PF00069	0.535
MOD_Plk_1	35	41	PF00069	0.552
MOD_Plk_1	392	398	PF00069	0.465
MOD_Plk_1	621	627	PF00069	0.433
MOD_Plk_1	648	654	PF00069	0.420
MOD_Plk_1	727	733	PF00069	0.496
MOD_Plk_1	794	800	PF00069	0.472
MOD_Plk_1	833	839	PF00069	0.549
MOD_Plk_1	876	882	PF00069	0.576
MOD_Plk_2-3	149	155	PF00069	0.450
MOD_Plk_2-3	834	840	PF00069	0.547
MOD_Plk_4	121	127	PF00069	0.514
MOD_Plk_4	141	147	PF00069	0.433
MOD_Plk_4	149	155	PF00069	0.481
MOD_Plk_4	216	222	PF00069	0.470
MOD_Plk_4	246	252	PF00069	0.477
MOD_Plk_4	307	313	PF00069	0.396
MOD_Plk_4	438	444	PF00069	0.429
MOD_Plk_4	476	482	PF00069	0.516
MOD_Plk_4	652	658	PF00069	0.502
MOD_Plk_4	683	689	PF00069	0.387
MOD_Plk_4	834	840	PF00069	0.541
MOD_Plk_4	99	105	PF00069	0.420
MOD_ProDKin_1	14	20	PF00069	0.538
MOD_ProDKin_1	585	591	PF00069	0.432
MOD_ProDKin_1	689	695	PF00069	0.563
MOD_ProDKin_1	763	769	PF00069	0.497
MOD_SUMO_rev_2	807	814	PF00179	0.504
TRG_DiLeu_BaEn_1	192	197	PF01217	0.456
TRG_DiLeu_BaEn_1	834	839	PF01217	0.546
TRG_DiLeu_BaLyEn_6	590	595	PF01217	0.383
TRG_ENDOCYTIC_2	210	213	PF00928	0.370
TRG_ENDOCYTIC_2	251	254	PF00928	0.428
TRG_ENDOCYTIC_2	324	327	PF00928	0.390
TRG_ENDOCYTIC_2	353	356	PF00928	0.609
TRG_ENDOCYTIC_2	398	401	PF00928	0.482
TRG_ENDOCYTIC_2	439	442	PF00928	0.507
TRG_ENDOCYTIC_2	737	740	PF00928	0.375
TRG_ER_diArg_1	348	351	PF00400	0.506
TRG_ER_diArg_1	383	385	PF00400	0.365
TRG_ER_diArg_1	55	58	PF00400	0.458
TRG_NLS_MonoExtC_3	908	914	PF00514	0.521
TRG_NLS_MonoExtN_4	907	913	PF00514	0.520
TRG_Pf-PMV_PEXEL_1	549	554	PF00026	0.347
TRG_Pf-PMV_PEXEL_1	910	914	PF00026	0.528

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1U2	Leptomonas seymouri	73%	100%
A0A0S4J991	Bodo saltans	38%	100%
A0A1X0P5L5	Trypanosomatidae	47%	100%
A0A3S7X9A3	Leishmania donovani	97%	100%
A0A422NM11	Trypanosoma rangeli	48%	100%
A4HMQ7	Leishmania braziliensis	91%	100%
A4IBC5	Leishmania infantum	97%	100%
B8ARW2	Oryza sativa subsp. indica	25%	100%
B9FDR3	Oryza sativa subsp. japonica	25%	100%
C9ZZC4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	98%
E9B6B3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O14089	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
O14787	Homo sapiens	25%	100%
Q3SYU7	Bos taurus	24%	100%
Q8BFY9	Mus musculus	25%	100%
Q8H0U4	Arabidopsis thaliana	28%	100%
Q92973	Homo sapiens	24%	100%
Q99LG2	Mus musculus	25%	100%
V5BTG7	Trypanosoma cruzi	47%	100%