Putative RNA-binding protein

Quick info Annotations Localization Phosphorylation Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA-binding protein, putative

Species:

Leishmania major

UniProt:

E9AF44_LEIMA

TriTrypDb:

LmjF.35.2200 , LMJLV39_350028800 , LMJSD75_350028100

Length:

274

Annotations

Annotations by Jardim et al.

Nucleic acid binding, RNA-binding

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	yes	yes: 12
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 21

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2
GO:1990904	ribonucleoprotein complex	2	2

Phosphorylation

Promastigote: 173
Promastigote/Amastigote: 10, 7

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

E9AF44

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF44

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003729	mRNA binding	5	2
GO:0005488	binding	1	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	102	104	PF00675	0.422
CLV_NRD_NRD_1	194	196	PF00675	0.362
CLV_NRD_NRD_1	24	26	PF00675	0.522
CLV_NRD_NRD_1	97	99	PF00675	0.228
CLV_PCSK_KEX2_1	102	104	PF00082	0.422
CLV_PCSK_KEX2_1	24	26	PF00082	0.538
CLV_PCSK_KEX2_1	97	99	PF00082	0.228
CLV_PCSK_PC7_1	98	104	PF00082	0.324
CLV_PCSK_SKI1_1	195	199	PF00082	0.245
DOC_CKS1_1	38	43	PF01111	0.419
DOC_CYCLIN_RxL_1	192	200	PF00134	0.376
DOC_MAPK_gen_1	230	239	PF00069	0.408
DOC_MAPK_gen_1	24	32	PF00069	0.500
DOC_MAPK_MEF2A_6	233	241	PF00069	0.408
DOC_PP4_FxxP_1	38	41	PF00568	0.436
DOC_USP7_MATH_1	141	145	PF00917	0.683
DOC_USP7_MATH_1	170	174	PF00917	0.547
DOC_USP7_MATH_1	68	72	PF00917	0.437
DOC_WW_Pin1_4	37	42	PF00397	0.419
LIG_14-3-3_CanoR_1	129	138	PF00244	0.600
LIG_APCC_ABBA_1	86	91	PF00400	0.408
LIG_CtBP_PxDLS_1	257	263	PF00389	0.408
LIG_FHA_1	177	183	PF00498	0.671
LIG_FHA_1	56	62	PF00498	0.408
LIG_FHA_2	90	96	PF00498	0.428
LIG_LIR_Gen_1	213	221	PF02991	0.408
LIG_LIR_Gen_1	40	49	PF02991	0.419
LIG_LIR_Nem_3	213	218	PF02991	0.408
LIG_LIR_Nem_3	254	258	PF02991	0.410
LIG_LIR_Nem_3	40	45	PF02991	0.439
LIG_Pex14_2	38	42	PF04695	0.408
LIG_PTB_Apo_2	198	205	PF02174	0.408
LIG_PTB_Apo_2	32	39	PF02174	0.408
LIG_SH2_STAP1	57	61	PF00017	0.408
LIG_SH2_STAT5	132	135	PF00017	0.643
LIG_SH2_STAT5	48	51	PF00017	0.452
LIG_SH2_STAT5	57	60	PF00017	0.414
LIG_SH3_3	105	111	PF00018	0.495
LIG_SH3_4	184	191	PF00018	0.532
LIG_SH3_CIN85_PxpxPR_1	181	186	PF14604	0.684
LIG_TYR_ITIM	55	60	PF00017	0.245
MOD_CK1_1	7	13	PF00069	0.579
MOD_CK2_1	241	247	PF00069	0.246
MOD_CK2_1	7	13	PF00069	0.673
MOD_CK2_1	89	95	PF00069	0.245
MOD_Cter_Amidation	193	196	PF01082	0.399
MOD_Cter_Amidation	95	98	PF01082	0.245
MOD_GlcNHglycan	143	146	PF01048	0.621
MOD_GlcNHglycan	157	160	PF01048	0.501
MOD_GlcNHglycan	208	211	PF01048	0.272
MOD_GlcNHglycan	70	73	PF01048	0.250
MOD_GSK3_1	121	128	PF00069	0.660
MOD_GSK3_1	68	75	PF00069	0.278
MOD_N-GLC_1	176	181	PF02516	0.697
MOD_N-GLC_1	89	94	PF02516	0.260
MOD_NEK2_1	155	160	PF00069	0.673
MOD_NEK2_1	197	202	PF00069	0.260
MOD_NEK2_1	204	209	PF00069	0.256
MOD_NEK2_1	211	216	PF00069	0.285
MOD_PIKK_1	184	190	PF00454	0.556
MOD_PKA_2	211	217	PF00069	0.245
MOD_PKA_2	4	10	PF00069	0.696
MOD_PKA_2	55	61	PF00069	0.245
MOD_Plk_4	26	32	PF00069	0.521
MOD_Plk_4	72	78	PF00069	0.304
MOD_ProDKin_1	37	43	PF00069	0.260
MOD_SUMO_for_1	242	245	PF00179	0.304
TRG_DiLeu_BaEn_1	95	100	PF01217	0.245
TRG_DiLeu_LyEn_5	95	100	PF01217	0.245
TRG_ENDOCYTIC_2	57	60	PF00928	0.271
TRG_ER_diArg_1	101	103	PF00400	0.407
TRG_ER_diArg_1	23	25	PF00400	0.653
TRG_ER_diArg_1	97	99	PF00400	0.271

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I120	Leptomonas seymouri	83%	100%
A0A0R4IEW8	Danio rerio	26%	67%
A0A0S4JGF6	Bodo saltans	54%	88%
A0A1X0P606	Trypanosomatidae	67%	98%
A0A3Q8IF50	Leishmania donovani	100%	100%
A0A422MNR3	Trypanosoma rangeli	68%	98%
A4HMP2	Leishmania braziliensis	93%	100%
A4QNI8	Xenopus tropicalis	25%	68%
B5DF91	Rattus norvegicus	21%	84%
C9ZZE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	91%
E9AHW1	Leishmania infantum	100%	100%
E9B699	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
P49314	Nicotiana plumbaginifolia	28%	94%
P70372	Mus musculus	21%	84%
Q08937	Nicotiana sylvestris	28%	94%
Q12926	Homo sapiens	25%	76%
Q14576	Homo sapiens	23%	75%
Q5R9Z6	Pongo abelii	25%	76%
Q60899	Mus musculus	24%	76%
Q60900	Mus musculus	23%	75%
Q7SZT7	Xenopus laevis	25%	68%
Q8CH84	Rattus norvegicus	25%	76%
Q91903	Xenopus laevis	23%	70%
V5BNY3	Trypanosoma cruzi	67%	99%