Putative R-SNARE protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

R-SNARE protein, putative

Species:

Leishmania major

UniProt:

E9AF35_LEIMA

TriTrypDb:

LmjF.35.2120 , LMJLV39_350027900 , LMJSD75_350027200

Length:

205

Annotations

LeishMANIAdb annotations

Homologous to animal VAMP7 proteins. Likely involved in vesicular transport.. Localization: Vesicular (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	2
GO:0005794	Golgi apparatus	5	2
GO:0016020	membrane	2	11
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

E9AF35

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF35

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	3
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	2
GO:0009987	cellular process	1	2
GO:0016192	vesicle-mediated transport	4	3
GO:0046907	intracellular transport	3	2
GO:0048193	Golgi vesicle transport	5	2
GO:0051179	localization	1	3
GO:0051234	establishment of localization	2	3
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2

Molecular functions
Term	Name	Level	Count
GO:0005484	SNAP receptor activity	3	2
GO:0030674	protein-macromolecule adaptor activity	2	2
GO:0060090	molecular adaptor activity	1	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_FUR_1	147	151	PF00082	0.433
CLV_PCSK_KEX2_1	149	151	PF00082	0.324
CLV_PCSK_KEX2_1	53	55	PF00082	0.368
CLV_PCSK_PC1ET2_1	149	151	PF00082	0.324
CLV_PCSK_PC1ET2_1	53	55	PF00082	0.326
CLV_PCSK_PC7_1	49	55	PF00082	0.350
CLV_PCSK_SKI1_1	54	58	PF00082	0.364
CLV_PCSK_SKI1_1	92	96	PF00082	0.305
DEG_Nend_UBRbox_1	1	4	PF02207	0.359
DOC_PP1_RVXF_1	90	96	PF00149	0.305
LIG_14-3-3_CanoR_1	37	45	PF00244	0.380
LIG_14-3-3_CanoR_1	92	98	PF00244	0.307
LIG_BRCT_BRCA1_1	186	190	PF00533	0.305
LIG_BRCT_BRCA1_1	30	34	PF00533	0.402
LIG_FHA_1	153	159	PF00498	0.305
LIG_GBD_Chelix_1	93	101	PF00786	0.324
LIG_LIR_Apic_2	134	139	PF02991	0.459
LIG_LIR_Gen_1	42	51	PF02991	0.332
LIG_LIR_Nem_3	30	35	PF02991	0.408
LIG_LIR_Nem_3	42	46	PF02991	0.355
LIG_NRBOX	2	8	PF00104	0.471
LIG_Pex14_2	105	109	PF04695	0.305
LIG_REV1ctd_RIR_1	32	41	PF16727	0.380
LIG_SH2_CRK	4	8	PF00017	0.448
LIG_SH2_NCK_1	113	117	PF00017	0.426
LIG_SH2_NCK_1	160	164	PF00017	0.305
LIG_SH2_NCK_1	71	75	PF00017	0.350
LIG_SH2_SRC	71	74	PF00017	0.433
LIG_SH2_STAT5	191	194	PF00017	0.384
LIG_SH3_3	8	14	PF00018	0.477
LIG_TYR_ITIM	158	163	PF00017	0.305
LIG_UBA3_1	145	149	PF00899	0.305
LIG_UBA3_1	6	10	PF00899	0.450
LIG_WRC_WIRS_1	29	34	PF05994	0.379
MOD_CAAXbox	202	205	PF01239	0.563
MOD_CK2_1	36	42	PF00069	0.384
MOD_CK2_1	82	88	PF00069	0.350
MOD_GlcNHglycan	198	201	PF01048	0.507
MOD_GlcNHglycan	24	27	PF01048	0.387
MOD_GlcNHglycan	38	41	PF01048	0.360
MOD_N-GLC_1	27	32	PF02516	0.289
MOD_NEK2_1	105	110	PF00069	0.307
MOD_NEK2_1	35	40	PF00069	0.454
MOD_NEK2_1	47	52	PF00069	0.354
MOD_NEK2_2	186	191	PF00069	0.305
MOD_PIKK_1	189	195	PF00454	0.388
MOD_PIKK_1	58	64	PF00454	0.324
MOD_PK_1	112	118	PF00069	0.547
MOD_PKA_2	36	42	PF00069	0.401
MOD_PKB_1	110	118	PF00069	0.350
MOD_Plk_1	27	33	PF00069	0.365
MOD_Plk_4	186	192	PF00069	0.307
MOD_SUMO_rev_2	151	158	PF00179	0.305
TRG_DiLeu_BaEn_2	41	47	PF01217	0.344
TRG_DiLeu_BaEn_3	140	146	PF01217	0.324
TRG_ENDOCYTIC_2	113	116	PF00928	0.417
TRG_ENDOCYTIC_2	160	163	PF00928	0.322
TRG_ENDOCYTIC_2	4	7	PF00928	0.399
TRG_ER_diArg_1	109	112	PF00400	0.280
TRG_Pf-PMV_PEXEL_1	150	154	PF00026	0.324

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2C0	Leptomonas seymouri	89%	100%
A0A0S4J646	Bodo saltans	60%	100%
A0A0S4JCC4	Bodo saltans	25%	88%
A0A1X0P617	Trypanosomatidae	79%	100%
A0A3R7M993	Trypanosoma rangeli	79%	100%
A0A3S7X9G1	Leishmania donovani	98%	100%
A4HMN3	Leishmania braziliensis	92%	100%
C9ZZF4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	80%	100%
E9AHV2	Leishmania infantum	98%	100%
E9B690	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O15498	Homo sapiens	38%	100%
O60073	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	37%	100%
P36015	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	33%	100%
P51809	Homo sapiens	23%	93%
P70280	Mus musculus	23%	93%
Q17QI5	Bos taurus	23%	93%
Q32N70	Xenopus laevis	35%	100%
Q3T000	Bos taurus	37%	100%
Q54ES8	Dictyostelium discoideum	33%	100%
Q5EGY4	Rattus norvegicus	37%	100%
Q5RF94	Pongo abelii	23%	93%
Q5ZL74	Gallus gallus	23%	93%
Q6BSL0	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	31%	100%
Q6C537	Yarrowia lipolytica (strain CLIB 122 / E 150)	34%	100%
Q6CSA2	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	33%	100%
Q6DDU7	Xenopus laevis	35%	100%
Q6FW27	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	30%	100%
Q6P816	Xenopus tropicalis	35%	100%
Q757A4	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	33%	100%
Q7ZUN8	Danio rerio	34%	100%
Q9CQW1	Mus musculus	37%	100%
Q9JHW5	Rattus norvegicus	23%	93%
Q9LVM9	Arabidopsis thaliana	38%	100%
Q9ZRD6	Arabidopsis thaliana	42%	100%