Putative kinesin

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Putative kinesin

Gene product:

kinesin, putative

Species:

Leishmania major

UniProt:

E9AF32_LEIMA

TriTrypDb:

LmjF.35.2090 , LMJLV39_350027400 , LMJSD75_350026700

Length:

698

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 30
NetGPI	no	yes: 0, no: 30

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	5
GO:0005871	kinesin complex	3	2
GO:0005874	microtubule	6	15
GO:0005875	microtubule associated complex	2	2
GO:0032991	protein-containing complex	1	2
GO:0099080	supramolecular complex	2	15
GO:0099081	supramolecular polymer	3	15
GO:0099512	supramolecular fiber	4	15
GO:0099513	polymeric cytoskeletal fiber	5	15
GO:0110165	cellular anatomical entity	1	18

Expansion

Sequence features

E9AF32

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF32

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0007017	microtubule-based process	2	31
GO:0007018	microtubule-based movement	3	31
GO:0009987	cellular process	1	31
GO:0030705	cytoskeleton-dependent intracellular transport	4	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	31
GO:0003774	cytoskeletal motor activity	1	31
GO:0003777	microtubule motor activity	2	31
GO:0003824	catalytic activity	1	14
GO:0005488	binding	1	31
GO:0005515	protein binding	2	31
GO:0005524	ATP binding	5	31
GO:0008017	microtubule binding	5	31
GO:0008092	cytoskeletal protein binding	3	31
GO:0008574	plus-end-directed microtubule motor activity	3	2
GO:0015631	tubulin binding	4	31
GO:0016462	pyrophosphatase activity	5	2
GO:0016787	hydrolase activity	2	14
GO:0016817	hydrolase activity, acting on acid anhydrides	3	2
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	2
GO:0016887	ATP hydrolysis activity	7	2
GO:0017076	purine nucleotide binding	4	31
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	2
GO:0030554	adenyl nucleotide binding	5	31
GO:0032553	ribonucleotide binding	3	31
GO:0032555	purine ribonucleotide binding	4	31
GO:0032559	adenyl ribonucleotide binding	5	31
GO:0035639	purine ribonucleoside triphosphate binding	4	31
GO:0036094	small molecule binding	2	31
GO:0043167	ion binding	2	31
GO:0043168	anion binding	3	31
GO:0097159	organic cyclic compound binding	2	31
GO:0097367	carbohydrate derivative binding	2	31
GO:0140657	ATP-dependent activity	1	31
GO:1901265	nucleoside phosphate binding	3	31
GO:1901363	heterocyclic compound binding	2	31

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	83	87	PF00656	0.319
CLV_MEL_PAP_1	365	371	PF00089	0.257
CLV_NRD_NRD_1	216	218	PF00675	0.312
CLV_NRD_NRD_1	462	464	PF00675	0.562
CLV_NRD_NRD_1	491	493	PF00675	0.567
CLV_NRD_NRD_1	627	629	PF00675	0.601
CLV_PCSK_KEX2_1	216	218	PF00082	0.258
CLV_PCSK_KEX2_1	271	273	PF00082	0.566
CLV_PCSK_KEX2_1	278	280	PF00082	0.626
CLV_PCSK_KEX2_1	462	464	PF00082	0.561
CLV_PCSK_KEX2_1	491	493	PF00082	0.568
CLV_PCSK_KEX2_1	652	654	PF00082	0.514
CLV_PCSK_PC1ET2_1	271	273	PF00082	0.566
CLV_PCSK_PC1ET2_1	278	280	PF00082	0.545
CLV_PCSK_PC1ET2_1	652	654	PF00082	0.514
CLV_PCSK_SKI1_1	106	110	PF00082	0.372
CLV_PCSK_SKI1_1	144	148	PF00082	0.326
CLV_PCSK_SKI1_1	16	20	PF00082	0.282
CLV_PCSK_SKI1_1	167	171	PF00082	0.294
CLV_PCSK_SKI1_1	198	202	PF00082	0.290
CLV_PCSK_SKI1_1	265	269	PF00082	0.595
CLV_PCSK_SKI1_1	317	321	PF00082	0.343
CLV_PCSK_SKI1_1	374	378	PF00082	0.310
CLV_PCSK_SKI1_1	386	390	PF00082	0.222
CLV_PCSK_SKI1_1	414	418	PF00082	0.437
CLV_PCSK_SKI1_1	482	486	PF00082	0.688
CLV_PCSK_SKI1_1	640	644	PF00082	0.525
CLV_PCSK_SKI1_1	652	656	PF00082	0.464
CLV_Separin_Metazoa	536	540	PF03568	0.344
DOC_CYCLIN_yClb5_NLxxxL_5	368	376	PF00134	0.332
DOC_MAPK_gen_1	144	154	PF00069	0.312
DOC_MAPK_gen_1	479	488	PF00069	0.754
DOC_MAPK_gen_1	517	526	PF00069	0.620
DOC_MAPK_MEF2A_6	147	156	PF00069	0.276
DOC_MAPK_MEF2A_6	8	15	PF00069	0.342
DOC_PP1_RVXF_1	426	432	PF00149	0.531
DOC_PP4_FxxP_1	304	307	PF00568	0.383
DOC_PP4_FxxP_1	380	383	PF00568	0.302
DOC_USP7_MATH_1	221	225	PF00917	0.406
DOC_USP7_MATH_1	247	251	PF00917	0.483
DOC_USP7_MATH_1	451	455	PF00917	0.607
DOC_USP7_MATH_1	484	488	PF00917	0.630
DOC_USP7_MATH_1	495	499	PF00917	0.490
DOC_USP7_MATH_1	55	59	PF00917	0.306
DOC_USP7_MATH_1	554	558	PF00917	0.540
DOC_USP7_MATH_1	572	576	PF00917	0.517
DOC_USP7_MATH_1	607	611	PF00917	0.540
DOC_USP7_UBL2_3	485	489	PF12436	0.599
DOC_WW_Pin1_4	391	396	PF00397	0.328
DOC_WW_Pin1_4	628	633	PF00397	0.603
LIG_14-3-3_CanoR_1	133	141	PF00244	0.337
LIG_14-3-3_CanoR_1	491	500	PF00244	0.638
LIG_14-3-3_CanoR_1	653	658	PF00244	0.561
LIG_14-3-3_CanoR_1	8	14	PF00244	0.419
LIG_Actin_WH2_2	362	379	PF00022	0.442
LIG_Actin_WH2_2	6	22	PF00022	0.316
LIG_APCC_ABBA_1	159	164	PF00400	0.317
LIG_APCC_ABBA_1	74	79	PF00400	0.302
LIG_APCC_ABBAyCdc20_2	468	474	PF00400	0.495
LIG_BIR_II_1	1	5	PF00653	0.637
LIG_BIR_III_4	496	500	PF00653	0.428
LIG_BRCT_BRCA1_1	127	131	PF00533	0.315
LIG_BRCT_BRCA1_1	57	61	PF00533	0.312
LIG_Clathr_ClatBox_1	234	238	PF01394	0.316
LIG_Clathr_ClatBox_1	60	64	PF01394	0.429
LIG_CSK_EPIYA_1	311	315	PF00017	0.385
LIG_deltaCOP1_diTrp_1	294	299	PF00928	0.462
LIG_FHA_1	105	111	PF00498	0.311
LIG_FHA_1	313	319	PF00498	0.456
LIG_FHA_1	371	377	PF00498	0.323
LIG_FHA_1	455	461	PF00498	0.672
LIG_FHA_1	481	487	PF00498	0.664
LIG_FHA_1	8	14	PF00498	0.434
LIG_FHA_2	397	403	PF00498	0.251
LIG_FHA_2	420	426	PF00498	0.492
LIG_FHA_2	625	631	PF00498	0.700
LIG_FHA_2	688	694	PF00498	0.601
LIG_FHA_2	70	76	PF00498	0.348
LIG_LIR_Apic_2	293	298	PF02991	0.683
LIG_LIR_Apic_2	302	307	PF02991	0.602
LIG_LIR_Apic_2	379	383	PF02991	0.252
LIG_LIR_Gen_1	107	116	PF02991	0.313
LIG_LIR_Gen_1	128	139	PF02991	0.351
LIG_LIR_Gen_1	584	594	PF02991	0.502
LIG_LIR_Gen_1	62	67	PF02991	0.354
LIG_LIR_LC3C_4	521	526	PF02991	0.346
LIG_LIR_Nem_3	107	111	PF02991	0.329
LIG_LIR_Nem_3	128	134	PF02991	0.345
LIG_LIR_Nem_3	361	367	PF02991	0.293
LIG_LIR_Nem_3	584	589	PF02991	0.448
LIG_LIR_Nem_3	62	66	PF02991	0.343
LIG_NRBOX	371	377	PF00104	0.306
LIG_PCNA_yPIPBox_3	163	173	PF02747	0.426
LIG_Pex14_1	295	299	PF04695	0.478
LIG_Pex14_2	63	67	PF04695	0.349
LIG_SH2_CRK	440	444	PF00017	0.502
LIG_SH2_SRC	191	194	PF00017	0.385
LIG_SH2_SRC	77	80	PF00017	0.338
LIG_SH2_STAP1	314	318	PF00017	0.404
LIG_SH2_STAP1	440	444	PF00017	0.502
LIG_SH2_STAP1	473	477	PF00017	0.625
LIG_SH2_STAP1	77	81	PF00017	0.355
LIG_SH2_STAT5	191	194	PF00017	0.410
LIG_SH2_STAT5	314	317	PF00017	0.230
LIG_SH2_STAT5	593	596	PF00017	0.613
LIG_SH2_STAT5	65	68	PF00017	0.430
LIG_SH3_2	52	57	PF14604	0.353
LIG_SH3_3	187	193	PF00018	0.308
LIG_SH3_3	49	55	PF00018	0.342
LIG_SUMO_SIM_anti_2	236	241	PF11976	0.385
LIG_SUMO_SIM_anti_2	250	255	PF11976	0.417
LIG_SUMO_SIM_anti_2	384	391	PF11976	0.292
LIG_SUMO_SIM_anti_2	588	593	PF11976	0.586
LIG_UBA3_1	590	598	PF00899	0.620
LIG_WRC_WIRS_1	60	65	PF05994	0.301
LIG_WRC_WIRS_1	654	659	PF05994	0.392
MOD_CK1_1	104	110	PF00069	0.331
MOD_CK1_1	219	225	PF00069	0.334
MOD_CK1_1	226	232	PF00069	0.296
MOD_CK1_1	274	280	PF00069	0.707
MOD_CK1_1	332	338	PF00069	0.359
MOD_CK1_1	344	350	PF00069	0.386
MOD_CK1_1	370	376	PF00069	0.326
MOD_CK1_1	381	387	PF00069	0.330
MOD_CK1_1	454	460	PF00069	0.685
MOD_CK1_1	59	65	PF00069	0.317
MOD_CK1_1	644	650	PF00069	0.568
MOD_CK2_1	154	160	PF00069	0.301
MOD_CK2_1	290	296	PF00069	0.456
MOD_CK2_1	355	361	PF00069	0.283
MOD_CK2_1	396	402	PF00069	0.321
MOD_CK2_1	419	425	PF00069	0.508
MOD_CK2_1	526	532	PF00069	0.492
MOD_CK2_1	607	613	PF00069	0.513
MOD_CK2_1	624	630	PF00069	0.666
MOD_CK2_1	687	693	PF00069	0.560
MOD_CK2_1	69	75	PF00069	0.347
MOD_GlcNHglycan	103	106	PF01048	0.345
MOD_GlcNHglycan	127	130	PF01048	0.290
MOD_GlcNHglycan	175	178	PF01048	0.488
MOD_GlcNHglycan	23	26	PF01048	0.458
MOD_GlcNHglycan	244	247	PF01048	0.491
MOD_GlcNHglycan	273	276	PF01048	0.632
MOD_GlcNHglycan	284	287	PF01048	0.693
MOD_GlcNHglycan	331	334	PF01048	0.347
MOD_GlcNHglycan	435	438	PF01048	0.559
MOD_GlcNHglycan	474	478	PF01048	0.678
MOD_GlcNHglycan	493	496	PF01048	0.656
MOD_GlcNHglycan	497	500	PF01048	0.624
MOD_GlcNHglycan	528	531	PF01048	0.560
MOD_GlcNHglycan	570	573	PF01048	0.539
MOD_GSK3_1	219	226	PF00069	0.342
MOD_GSK3_1	337	344	PF00069	0.342
MOD_GSK3_1	363	370	PF00069	0.314
MOD_GSK3_1	378	385	PF00069	0.324
MOD_GSK3_1	396	403	PF00069	0.330
MOD_GSK3_1	480	487	PF00069	0.692
MOD_GSK3_1	491	498	PF00069	0.529
MOD_GSK3_1	55	62	PF00069	0.297
MOD_GSK3_1	568	575	PF00069	0.428
MOD_GSK3_1	624	631	PF00069	0.629
MOD_N-GLC_1	260	265	PF02516	0.677
MOD_N-GLC_1	345	350	PF02516	0.323
MOD_N-GLC_1	370	375	PF02516	0.336
MOD_N-GLC_1	674	679	PF02516	0.614
MOD_N-GLC_1	92	97	PF02516	0.306
MOD_NEK2_1	123	128	PF00069	0.393
MOD_NEK2_1	254	259	PF00069	0.572
MOD_NEK2_1	299	304	PF00069	0.513
MOD_NEK2_1	400	405	PF00069	0.341
MOD_NEK2_1	416	421	PF00069	0.467
MOD_NEK2_1	433	438	PF00069	0.534
MOD_NEK2_1	568	573	PF00069	0.570
MOD_NEK2_2	41	46	PF00069	0.351
MOD_NEK2_2	484	489	PF00069	0.632
MOD_NEK2_2	554	559	PF00069	0.572
MOD_PIKK_1	223	229	PF00454	0.442
MOD_PIKK_1	393	399	PF00454	0.336
MOD_PIKK_1	451	457	PF00454	0.622
MOD_PIKK_1	561	567	PF00454	0.664
MOD_PIKK_1	69	75	PF00454	0.306
MOD_PKA_1	216	222	PF00069	0.291
MOD_PKA_1	271	277	PF00069	0.756
MOD_PKA_1	491	497	PF00069	0.619
MOD_PKA_2	216	222	PF00069	0.306
MOD_PKA_2	271	277	PF00069	0.725
MOD_PKA_2	367	373	PF00069	0.317
MOD_PKA_2	491	497	PF00069	0.616
MOD_PKA_2	7	13	PF00069	0.379
MOD_Plk_1	198	204	PF00069	0.322
MOD_Plk_1	340	346	PF00069	0.335
MOD_Plk_1	370	376	PF00069	0.325
MOD_Plk_1	400	406	PF00069	0.258
MOD_Plk_1	41	47	PF00069	0.306
MOD_Plk_1	674	680	PF00069	0.382
MOD_Plk_1	69	75	PF00069	0.322
MOD_Plk_1	92	98	PF00069	0.284
MOD_Plk_4	191	197	PF00069	0.305
MOD_Plk_4	299	305	PF00069	0.502
MOD_Plk_4	467	473	PF00069	0.642
MOD_Plk_4	56	62	PF00069	0.333
MOD_Plk_4	585	591	PF00069	0.556
MOD_Plk_4	674	680	PF00069	0.396
MOD_Plk_4	92	98	PF00069	0.304
MOD_ProDKin_1	391	397	PF00069	0.328
MOD_ProDKin_1	628	634	PF00069	0.594
MOD_SUMO_for_1	45	48	PF00179	0.351
MOD_SUMO_rev_2	136	143	PF00179	0.327
MOD_SUMO_rev_2	257	266	PF00179	0.617
MOD_SUMO_rev_2	544	551	PF00179	0.564
MOD_SUMO_rev_2	633	642	PF00179	0.667
MOD_SUMO_rev_2	693	698	PF00179	0.674
TRG_DiLeu_BaEn_1	507	512	PF01217	0.586
TRG_DiLeu_BaLyEn_6	371	376	PF01217	0.302
TRG_ENDOCYTIC_2	440	443	PF00928	0.538
TRG_ER_diArg_1	13	16	PF00400	0.323
TRG_ER_diArg_1	216	218	PF00400	0.404
TRG_ER_diArg_1	461	463	PF00400	0.566
TRG_ER_diArg_1	491	493	PF00400	0.720
TRG_NES_CRM1_1	441	453	PF08389	0.491
TRG_NLS_MonoExtC_3	681	687	PF00514	0.378
TRG_NLS_MonoExtN_4	680	686	PF00514	0.403
TRG_Pf-PMV_PEXEL_1	133	137	PF00026	0.266
TRG_Pf-PMV_PEXEL_1	463	467	PF00026	0.704
TRG_Pf-PMV_PEXEL_1	491	496	PF00026	0.658

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P569	Leptomonas seymouri	24%	80%
A0A0N0P672	Leptomonas seymouri	33%	66%
A0A0N1HVM1	Leptomonas seymouri	29%	75%
A0A0N1IM30	Leptomonas seymouri	71%	100%
A0A0S4IXS6	Bodo saltans	40%	68%
A0A0S4JEF6	Bodo saltans	26%	100%
A0A0S4JN49	Bodo saltans	50%	96%
A0A1X0P5Y8	Trypanosomatidae	60%	100%
A0A3Q8IBS7	Leishmania donovani	25%	100%
A0A3Q8IG88	Leishmania donovani	92%	100%
A0A3S7WX05	Leishmania donovani	29%	79%
A0A422MZ05	Trypanosoma rangeli	56%	100%
A0A422NEF2	Trypanosoma rangeli	35%	69%
A0A422NEQ8	Trypanosoma rangeli	26%	92%
A4HCA1	Leishmania braziliensis	31%	78%
A4HHY2	Leishmania braziliensis	27%	100%
A4HMM9	Leishmania braziliensis	75%	100%
A4HZT3	Leishmania infantum	29%	79%
A4I562	Leishmania infantum	25%	100%
A4IBA7	Leishmania infantum	92%	100%
C9ZU98	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	86%
C9ZVT5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	69%
C9ZZN5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
E9B0F9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9B687	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
Q4QBU1	Leishmania major	31%	78%
V5B8X9	Trypanosoma cruzi	57%	99%
V5D733	Trypanosoma cruzi	25%	93%
V5DFA7	Trypanosoma cruzi	28%	100%