Putative ER--golgi transport protein gp25L

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

COP-coated vesicle membrane protein gp25L precursor, putative

Species:

Leishmania major

UniProt:

E9AF06_LEIMA

TriTrypDb:

LmjF.35.1850 , LMJLV39_350024900 , LMJSD75_350024100

Length:

216

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 8, no: 2
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	2
GO:0005794	Golgi apparatus	5	2
GO:0016020	membrane	2	11
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	2
GO:0030135	coated vesicle	7	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

E9AF06

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF06

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	2
GO:0006996	organelle organization	4	2
GO:0007030	Golgi organization	5	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016043	cellular component organization	3	2
GO:0016192	vesicle-mediated transport	4	2
GO:0033036	macromolecule localization	2	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0048193	Golgi vesicle transport	5	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0071840	cellular component organization or biogenesis	2	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	3	5	PF00675	0.699
CLV_PCSK_KEX2_1	206	208	PF00082	0.250
CLV_PCSK_KEX2_1	212	214	PF00082	0.339
CLV_PCSK_KEX2_1	3	5	PF00082	0.695
CLV_PCSK_PC1ET2_1	206	208	PF00082	0.251
CLV_PCSK_PC1ET2_1	212	214	PF00082	0.338
CLV_PCSK_SKI1_1	117	121	PF00082	0.518
CLV_PCSK_SKI1_1	203	207	PF00082	0.259
CLV_PCSK_SKI1_1	41	45	PF00082	0.530
DEG_APCC_DBOX_1	116	124	PF00400	0.311
DEG_APCC_DBOX_1	40	48	PF00400	0.216
DEG_SPOP_SBC_1	23	27	PF00917	0.387
DOC_MAPK_gen_1	114	122	PF00069	0.240
DOC_MAPK_gen_1	138	146	PF00069	0.229
DOC_MAPK_MEF2A_6	114	123	PF00069	0.234
DOC_MAPK_MEF2A_6	140	148	PF00069	0.217
DOC_MAPK_MEF2A_6	182	190	PF00069	0.384
DOC_PP1_RVXF_1	180	186	PF00149	0.272
DOC_USP7_UBL2_3	206	210	PF12436	0.511
LIG_14-3-3_CanoR_1	3	13	PF00244	0.553
LIG_Actin_WH2_2	40	56	PF00022	0.298
LIG_BRCT_BRCA1_1	100	104	PF00533	0.159
LIG_BRCT_BRCA1_1	39	43	PF00533	0.311
LIG_BRCT_BRCA1_1	86	90	PF00533	0.233
LIG_FHA_1	114	120	PF00498	0.292
LIG_FHA_1	12	18	PF00498	0.461
LIG_FHA_1	178	184	PF00498	0.235
LIG_FHA_2	152	158	PF00498	0.306
LIG_FHA_2	60	66	PF00498	0.311
LIG_LIR_Gen_1	129	136	PF02991	0.230
LIG_LIR_Gen_1	161	170	PF02991	0.280
LIG_LIR_Gen_1	65	74	PF02991	0.311
LIG_LIR_Nem_3	161	165	PF02991	0.231
LIG_LIR_Nem_3	60	66	PF02991	0.259
LIG_Pex14_2	194	198	PF04695	0.388
LIG_SH2_STAP1	100	104	PF00017	0.172
LIG_SH2_STAP1	132	136	PF00017	0.247
LIG_SH2_STAT5	164	167	PF00017	0.234
LIG_SH2_STAT5	199	202	PF00017	0.384
LIG_SH2_STAT5	208	211	PF00017	0.416
LIG_SUMO_SIM_anti_2	118	124	PF11976	0.233
LIG_SUMO_SIM_anti_2	65	72	PF11976	0.265
LIG_SUMO_SIM_par_1	19	27	PF11976	0.423
LIG_TRAF2_1	154	157	PF00917	0.265
MOD_CK1_1	37	43	PF00069	0.265
MOD_CK2_1	151	157	PF00069	0.326
MOD_Cter_Amidation	1	4	PF01082	0.566
MOD_GlcNHglycan	111	114	PF01048	0.561
MOD_GlcNHglycan	36	39	PF01048	0.530
MOD_GlcNHglycan	48	51	PF01048	0.406
MOD_GlcNHglycan	7	10	PF01048	0.592
MOD_GlcNHglycan	86	89	PF01048	0.551
MOD_GSK3_1	109	116	PF00069	0.298
MOD_GSK3_1	98	105	PF00069	0.269
MOD_NEK2_1	102	107	PF00069	0.349
MOD_NEK2_1	115	120	PF00069	0.247
MOD_NEK2_1	151	156	PF00069	0.288
MOD_NEK2_1	184	189	PF00069	0.342
MOD_NEK2_1	22	27	PF00069	0.434
MOD_NEK2_1	83	88	PF00069	0.290
MOD_PKA_1	3	9	PF00069	0.605
MOD_PKA_2	172	178	PF00069	0.272
MOD_PKA_2	3	9	PF00069	0.560
MOD_PKA_2	92	98	PF00069	0.293
MOD_Plk_1	151	157	PF00069	0.257
MOD_Plk_1	59	65	PF00069	0.306
MOD_Plk_1	98	104	PF00069	0.214
TRG_ENDOCYTIC_2	132	135	PF00928	0.216
TRG_ENDOCYTIC_2	199	202	PF00928	0.365
TRG_ENDOCYTIC_2	63	66	PF00928	0.230
TRG_ENDOCYTIC_2	80	83	PF00928	0.206
TRG_ER_diArg_1	165	168	PF00400	0.265
TRG_NES_CRM1_1	139	153	PF08389	0.229
TRG_NLS_MonoCore_2	209	214	PF00514	0.569
TRG_NLS_MonoExtC_3	210	216	PF00514	0.572
TRG_NLS_MonoExtN_4	207	214	PF00514	0.548

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBE2	Leptomonas seymouri	61%	100%
A0A0S4IQL9	Bodo saltans	42%	100%
A0A0S4J7E6	Bodo saltans	24%	98%
A0A0S4JYK0	Bodo saltans	24%	100%
A0A1X0NVX2	Trypanosomatidae	21%	95%
A0A1X0P6V5	Trypanosomatidae	44%	100%
A0A3R7NST9	Trypanosoma rangeli	44%	100%
A0A3S7X951	Leishmania donovani	96%	100%
A0A422NB76	Trypanosoma rangeli	23%	98%
A0AQ71	Drosophila simulans	26%	100%
A4HMK6	Leishmania braziliensis	82%	100%
A4IB85	Leishmania infantum	96%	100%
A7SXK3	Nematostella vectensis	23%	100%
B3LVB0	Drosophila ananassae	25%	100%
B3MTS8	Drosophila ananassae	26%	100%
B3NZM5	Drosophila erecta	26%	100%
B3P6T8	Drosophila erecta	27%	100%
B4GE47	Drosophila persimilis	27%	100%
B4GMC3	Drosophila persimilis	26%	100%
B4HJV5	Drosophila sechellia	27%	100%
B4JG34	Drosophila grimshawi	27%	100%
B4JYU5	Drosophila grimshawi	26%	100%
B4K4G5	Drosophila mojavensis	26%	100%
B4KB41	Drosophila mojavensis	27%	100%
B4LYB8	Drosophila virilis	27%	100%
B4MGF8	Drosophila virilis	26%	100%
B4NIY1	Drosophila willistoni	26%	100%
B4NKL0	Drosophila willistoni	26%	100%
B4PUZ3	Drosophila yakuba	27%	100%
B4PVC6	Drosophila yakuba	26%	100%
B4QWH9	Drosophila simulans	26%	100%
C9ZZL0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	99%
D0AA90	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	23%	95%
D3ZTX0	Rattus norvegicus	25%	96%
E9B662	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
F4J4Y0	Arabidopsis thaliana	29%	100%
O13946	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
O14324	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
O17528	Caenorhabditis elegans	22%	100%
O35587	Mesocricetus auratus	29%	99%
O81045	Arabidopsis thaliana	26%	100%
P27869	Canis lupus familiaris	22%	100%
P38819	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	100%
P49020	Cricetulus griseus	24%	100%
P49755	Homo sapiens	28%	99%
P53198	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
Q05359	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	99%
Q13445	Homo sapiens	25%	95%
Q15363	Homo sapiens	23%	100%
Q28735	Oryctolagus cuniculus	28%	99%
Q28BQ6	Xenopus tropicalis	23%	98%
Q295B2	Drosophila pseudoobscura pseudoobscura	26%	100%
Q2TBK5	Bos taurus	27%	95%
Q3T133	Bos taurus	26%	92%
Q3V009	Mus musculus	26%	95%
Q4HY20	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	30%	100%
Q54BN0	Dictyostelium discoideum	24%	100%
Q5A302	Candida albicans (strain SC5314 / ATCC MYA-2876)	29%	100%
Q5B5L5	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	32%	100%
Q5BK85	Rattus norvegicus	26%	95%
Q5E971	Bos taurus	28%	99%
Q5I0E7	Rattus norvegicus	26%	92%
Q5R7E6	Pongo abelii	25%	95%
Q5RE32	Pongo abelii	28%	99%
Q63524	Rattus norvegicus	23%	100%
Q63584	Rattus norvegicus	28%	99%
Q6AY25	Rattus norvegicus	26%	98%
Q6BTC2	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	31%	100%
Q6C503	Yarrowia lipolytica (strain CLIB 122 / E 150)	27%	100%
Q6CWW7	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	24%	100%
Q6FRZ3	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	30%	100%
Q6IDL4	Arabidopsis thaliana	28%	100%
Q759T8	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	26%	100%
Q769F9	Dictyostelium discoideum	27%	100%
Q78IS1	Mus musculus	25%	98%
Q7Z7H5	Homo sapiens	26%	95%
Q8GYG1	Arabidopsis thaliana	28%	100%
Q8R1V4	Mus musculus	26%	95%
Q8RWM6	Arabidopsis thaliana	29%	100%
Q8SXY6	Drosophila melanogaster	25%	100%
Q8VY92	Arabidopsis thaliana	27%	100%
Q90515	Takifugu rubripes	28%	100%
Q99KF1	Mus musculus	26%	92%
Q9BVK6	Homo sapiens	27%	92%
Q9D1D4	Mus musculus	29%	99%
Q9D2R4	Mus musculus	24%	100%
Q9FVU0	Arabidopsis thaliana	29%	100%
Q9HEK4	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	31%	100%
Q9I7K5	Drosophila melanogaster	26%	100%
Q9LIL4	Arabidopsis thaliana	24%	100%
Q9LJV9	Arabidopsis thaliana	23%	96%
Q9LQY3	Arabidopsis thaliana	26%	100%
Q9P7I9	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
Q9R0Q3	Mus musculus	23%	100%
Q9Y3B3	Homo sapiens	25%	96%
Q9Y3Q3	Homo sapiens	26%	100%
V5BG96	Trypanosoma cruzi	22%	98%