Trypanin-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

nexin-dynein regulatory complex 4

Species:

Leishmania major

UniProt:

E9AF02_LEIMA

TriTrypDb:

LmjF.35.1810 , LMJLV39_350024500 , LMJSD75_350023700

Length:

461

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005794	Golgi apparatus	5	2
GO:0005874	microtubule	6	2
GO:0005929	cilium	4	12
GO:0005930	axoneme	2	2
GO:0031514	motile cilium	5	12
GO:0042995	cell projection	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0099080	supramolecular complex	2	2
GO:0099081	supramolecular polymer	3	2
GO:0099512	supramolecular fiber	4	2
GO:0099513	polymeric cytoskeletal fiber	5	2
GO:0110165	cellular anatomical entity	1	12
GO:0120025	plasma membrane bounded cell projection	3	12
GO:0005737	cytoplasm	2	10
GO:0005856	cytoskeleton	5	10
GO:0043228	non-membrane-bounded organelle	3	10
GO:0043232	intracellular non-membrane-bounded organelle	4	10

Expansion

Sequence features

E9AF02

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AF02

Function

Biological processes
Term	Name	Level	Count
GO:0001539	cilium or flagellum-dependent cell motility	3	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	12
GO:0033036	macromolecule localization	2	2
GO:0043393	regulation of protein binding	4	2
GO:0048870	cell motility	2	12
GO:0051098	regulation of binding	3	2
GO:0051179	localization	1	12
GO:0051641	cellular localization	2	2
GO:0060285	cilium-dependent cell motility	4	2
GO:0065007	biological regulation	1	2
GO:0065009	regulation of molecular function	2	2
GO:0070727	cellular macromolecule localization	3	2
GO:1904526	regulation of microtubule binding	5	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0008017	microtubule binding	5	12
GO:0008092	cytoskeletal protein binding	3	12
GO:0015631	tubulin binding	4	12
GO:0019899	enzyme binding	3	12
GO:0031267	small GTPase binding	5	12
GO:0051020	GTPase binding	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	375	379	PF00656	0.413
CLV_NRD_NRD_1	199	201	PF00675	0.402
CLV_NRD_NRD_1	209	211	PF00675	0.363
CLV_NRD_NRD_1	253	255	PF00675	0.203
CLV_NRD_NRD_1	353	355	PF00675	0.243
CLV_NRD_NRD_1	359	361	PF00675	0.208
CLV_NRD_NRD_1	4	6	PF00675	0.771
CLV_PCSK_FUR_1	271	275	PF00082	0.290
CLV_PCSK_KEX2_1	255	257	PF00082	0.203
CLV_PCSK_KEX2_1	273	275	PF00082	0.203
CLV_PCSK_KEX2_1	307	309	PF00082	0.307
CLV_PCSK_KEX2_1	359	361	PF00082	0.214
CLV_PCSK_KEX2_1	386	388	PF00082	0.214
CLV_PCSK_KEX2_1	4	6	PF00082	0.557
CLV_PCSK_KEX2_1	63	65	PF00082	0.479
CLV_PCSK_PC1ET2_1	255	257	PF00082	0.203
CLV_PCSK_PC1ET2_1	273	275	PF00082	0.203
CLV_PCSK_PC1ET2_1	307	309	PF00082	0.307
CLV_PCSK_PC1ET2_1	386	388	PF00082	0.228
CLV_PCSK_PC1ET2_1	4	6	PF00082	0.557
CLV_PCSK_PC1ET2_1	63	65	PF00082	0.479
CLV_PCSK_SKI1_1	118	122	PF00082	0.538
CLV_PCSK_SKI1_1	157	161	PF00082	0.483
CLV_PCSK_SKI1_1	176	180	PF00082	0.264
CLV_PCSK_SKI1_1	245	249	PF00082	0.240
CLV_PCSK_SKI1_1	273	277	PF00082	0.208
CLV_PCSK_SKI1_1	286	290	PF00082	0.203
CLV_PCSK_SKI1_1	300	304	PF00082	0.172
CLV_PCSK_SKI1_1	308	312	PF00082	0.192
CLV_PCSK_SKI1_1	330	334	PF00082	0.244
CLV_PCSK_SKI1_1	355	359	PF00082	0.208
CLV_PCSK_SKI1_1	377	381	PF00082	0.300
CLV_PCSK_SKI1_1	417	421	PF00082	0.453
CLV_PCSK_SKI1_1	91	95	PF00082	0.368
CLV_PCSK_SKI1_1	99	103	PF00082	0.343
DOC_CYCLIN_RxL_1	151	164	PF00134	0.518
DOC_CYCLIN_RxL_1	281	294	PF00134	0.418
DOC_CYCLIN_RxL_1	359	372	PF00134	0.507
DOC_CYCLIN_RxL_1	95	104	PF00134	0.446
DOC_MAPK_gen_1	286	293	PF00069	0.484
DOC_MAPK_MEF2A_6	286	293	PF00069	0.494
DOC_MAPK_NFAT4_5	286	294	PF00069	0.418
DOC_PP1_RVXF_1	415	422	PF00149	0.478
DOC_PP1_RVXF_1	52	59	PF00149	0.366
DOC_USP7_MATH_1	119	123	PF00917	0.571
DOC_USP7_MATH_1	14	18	PF00917	0.681
DOC_USP7_MATH_1	8	12	PF00917	0.712
DOC_USP7_UBL2_3	307	311	PF12436	0.481
DOC_USP7_UBL2_3	76	80	PF12436	0.531
DOC_USP7_UBL2_3	91	95	PF12436	0.309
DOC_WW_Pin1_4	16	21	PF00397	0.566
LIG_14-3-3_CanoR_1	118	127	PF00244	0.493
LIG_14-3-3_CanoR_1	157	167	PF00244	0.488
LIG_14-3-3_CanoR_1	274	278	PF00244	0.472
LIG_14-3-3_CanoR_1	354	362	PF00244	0.470
LIG_14-3-3_CanoR_1	422	431	PF00244	0.490
LIG_Actin_WH2_2	174	192	PF00022	0.492
LIG_FHA_1	274	280	PF00498	0.403
LIG_FHA_1	33	39	PF00498	0.406
LIG_FHA_1	380	386	PF00498	0.440
LIG_FHA_2	109	115	PF00498	0.556
LIG_FHA_2	119	125	PF00498	0.526
LIG_FHA_2	159	165	PF00498	0.480
LIG_FHA_2	223	229	PF00498	0.414
LIG_FHA_2	244	250	PF00498	0.403
LIG_FHA_2	257	263	PF00498	0.403
LIG_FHA_2	311	317	PF00498	0.533
LIG_FHA_2	370	376	PF00498	0.470
LIG_FHA_2	410	416	PF00498	0.470
LIG_FHA_2	423	429	PF00498	0.434
LIG_LIR_Gen_1	223	233	PF02991	0.494
LIG_LIR_Gen_1	328	335	PF02991	0.421
LIG_LIR_Nem_3	223	229	PF02991	0.470
LIG_LIR_Nem_3	231	236	PF02991	0.403
LIG_LIR_Nem_3	328	334	PF02991	0.418
LIG_LIR_Nem_3	356	361	PF02991	0.428
LIG_LIR_Nem_3	433	437	PF02991	0.429
LIG_NRBOX	380	386	PF00104	0.414
LIG_NRBOX	415	421	PF00104	0.526
LIG_PDZ_Class_2	456	461	PF00595	0.564
LIG_SH2_CRK	331	335	PF00017	0.545
LIG_SH2_GRB2like	103	106	PF00017	0.505
LIG_SH2_NCK_1	266	270	PF00017	0.507
LIG_SH2_STAP1	103	107	PF00017	0.357
LIG_SH2_STAP1	185	189	PF00017	0.400
LIG_SH2_STAP1	331	335	PF00017	0.507
LIG_SH2_STAP1	434	438	PF00017	0.389
LIG_SH2_STAT3	103	106	PF00017	0.360
LIG_SH2_STAT5	232	235	PF00017	0.507
LIG_SH2_STAT5	296	299	PF00017	0.470
LIG_SH2_STAT5	47	50	PF00017	0.369
LIG_TRAF2_1	122	125	PF00917	0.530
LIG_TRAF2_1	313	316	PF00917	0.481
LIG_TYR_ITIM	183	188	PF00017	0.508
LIG_UBA3_1	348	355	PF00899	0.403
MOD_CK1_1	234	240	PF00069	0.430
MOD_CK2_1	108	114	PF00069	0.432
MOD_CK2_1	118	124	PF00069	0.436
MOD_CK2_1	169	175	PF00069	0.508
MOD_CK2_1	188	194	PF00069	0.292
MOD_CK2_1	222	228	PF00069	0.437
MOD_CK2_1	256	262	PF00069	0.507
MOD_CK2_1	310	316	PF00069	0.511
MOD_CK2_1	369	375	PF00069	0.443
MOD_CK2_1	422	428	PF00069	0.491
MOD_CK2_1	433	439	PF00069	0.412
MOD_CK2_1	59	65	PF00069	0.381
MOD_CK2_1	69	75	PF00069	0.449
MOD_GlcNHglycan	10	13	PF01048	0.644
MOD_GlcNHglycan	16	19	PF01048	0.602
MOD_GlcNHglycan	355	358	PF01048	0.312
MOD_GlcNHglycan	443	446	PF01048	0.485
MOD_GlcNHglycan	449	452	PF01048	0.637
MOD_GlcNHglycan	453	456	PF01048	0.607
MOD_GlcNHglycan	6	9	PF01048	0.631
MOD_GSK3_1	126	133	PF00069	0.596
MOD_GSK3_1	218	225	PF00069	0.486
MOD_GSK3_1	231	238	PF00069	0.415
MOD_GSK3_1	306	313	PF00069	0.450
MOD_GSK3_1	4	11	PF00069	0.637
MOD_GSK3_1	447	454	PF00069	0.625
MOD_LATS_1	252	258	PF00433	0.356
MOD_N-GLC_1	59	64	PF02516	0.491
MOD_NEK2_1	138	143	PF00069	0.518
MOD_NEK2_1	188	193	PF00069	0.511
MOD_NEK2_1	369	374	PF00069	0.433
MOD_NEK2_1	38	43	PF00069	0.453
MOD_NEK2_1	421	426	PF00069	0.454
MOD_NEK2_2	455	460	PF00069	0.514
MOD_PIKK_1	113	119	PF00454	0.322
MOD_PIKK_1	158	164	PF00454	0.522
MOD_PIKK_1	310	316	PF00454	0.507
MOD_PIKK_1	325	331	PF00454	0.358
MOD_PIKK_1	369	375	PF00454	0.414
MOD_PIKK_1	422	428	PF00454	0.404
MOD_PKA_1	254	260	PF00069	0.403
MOD_PKA_1	273	279	PF00069	0.403
MOD_PKA_1	4	10	PF00069	0.489
MOD_PKA_2	138	144	PF00069	0.479
MOD_PKA_2	218	224	PF00069	0.439
MOD_PKA_2	273	279	PF00069	0.507
MOD_PKA_2	353	359	PF00069	0.507
MOD_PKA_2	4	10	PF00069	0.489
MOD_PKA_2	421	427	PF00069	0.484
MOD_PKB_1	254	262	PF00069	0.500
MOD_Plk_1	222	228	PF00069	0.414
MOD_Plk_1	409	415	PF00069	0.438
MOD_Plk_2-3	404	410	PF00069	0.507
MOD_Plk_4	433	439	PF00069	0.480
MOD_ProDKin_1	16	22	PF00069	0.565
MOD_SUMO_for_1	112	115	PF00179	0.476
MOD_SUMO_for_1	247	250	PF00179	0.403
MOD_SUMO_for_1	280	283	PF00179	0.418
MOD_SUMO_rev_2	172	181	PF00179	0.394
MOD_SUMO_rev_2	299	309	PF00179	0.455
MOD_SUMO_rev_2	375	382	PF00179	0.478
MOD_SUMO_rev_2	393	402	PF00179	0.507
MOD_SUMO_rev_2	410	419	PF00179	0.450
TRG_ENDOCYTIC_2	185	188	PF00928	0.509
TRG_ENDOCYTIC_2	331	334	PF00928	0.457
TRG_ENDOCYTIC_2	345	348	PF00928	0.360
TRG_ENDOCYTIC_2	47	50	PF00928	0.369
TRG_ER_diArg_1	358	360	PF00400	0.427
TRG_NLS_MonoExtN_4	2	8	PF00514	0.654
TRG_Pf-PMV_PEXEL_1	136	140	PF00026	0.516
TRG_Pf-PMV_PEXEL_1	151	155	PF00026	0.462
TRG_Pf-PMV_PEXEL_1	157	162	PF00026	0.474
TRG_Pf-PMV_PEXEL_1	176	180	PF00026	0.282
TRG_Pf-PMV_PEXEL_1	245	249	PF00026	0.203
TRG_Pf-PMV_PEXEL_1	273	277	PF00026	0.203
TRG_Pf-PMV_PEXEL_1	360	364	PF00026	0.224
TRG_Pf-PMV_PEXEL_1	99	104	PF00026	0.445

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HWE1	Leptomonas seymouri	75%	96%
A0A0N1HYA1	Leptomonas seymouri	31%	100%
A0A0S4IRB6	Bodo saltans	53%	100%
A0A0S4J2F6	Bodo saltans	28%	100%
A0A1X0P612	Trypanosomatidae	62%	97%
A0A1X0P7Y2	Trypanosomatidae	30%	100%
A0A3S5H7Z1	Leishmania donovani	98%	100%
A0A3S7XAT7	Leishmania donovani	29%	100%
A0A422MVC9	Trypanosoma rangeli	32%	100%
A0A422NNP7	Trypanosoma rangeli	58%	100%
A4HMK2	Leishmania braziliensis	89%	100%
A4HP56	Leishmania braziliensis	28%	100%
A4IB81	Leishmania infantum	98%	100%
A4IDG2	Leishmania infantum	29%	100%
A5D7M3	Bos taurus	34%	96%
C9ZZK6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	100%
D0A342	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9ASW4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9B658	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
F1QNW4	Danio rerio	33%	97%
O15697	Trypanosoma brucei rhodesiense	30%	100%
O95995	Homo sapiens	35%	96%
Q499U4	Rattus norvegicus	34%	96%
Q4Q1N3	Leishmania major	30%	100%
Q60779	Mus musculus	34%	96%
Q7XJ96	Chlamydomonas reinhardtii	37%	98%
Q8MT08	Drosophila melanogaster	28%	96%
V5ATF4	Trypanosoma cruzi	59%	100%
V5BTX8	Trypanosoma cruzi	32%	94%