Pru domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

proteasome complex subunit Rpn13 ubiquitin receptor, putative

Species:

Leishmania major

UniProt:

E9AEZ8_LEIMA

TriTrypDb:

LmjF.35.1760 , LMJLV39_350024100 , LMJSD75_350023300

Length:

246

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 14
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	2
GO:0005730	nucleolus	5	2
GO:0005737	cytoplasm	2	15
GO:0008541	proteasome regulatory particle, lid subcomplex	2	2
GO:0032991	protein-containing complex	1	8
GO:0043226	organelle	2	15
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	15
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	15
GO:0000502	proteasome complex	3	6
GO:0005634	nucleus	5	13
GO:0043227	membrane-bounded organelle	3	13
GO:0043231	intracellular membrane-bounded organelle	4	13
GO:0140535	intracellular protein-containing complex	2	6
GO:1902494	catalytic complex	2	6
GO:1905368	peptidase complex	3	6
GO:1905369	endopeptidase complex	4	6

Expansion

Sequence features

E9AEZ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEZ8

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0019538	protein metabolic process	3	2
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0008047	enzyme activator activity	3	2
GO:0016504	peptidase activator activity	4	2
GO:0030234	enzyme regulator activity	2	2
GO:0032182	ubiquitin-like protein binding	3	2
GO:0043130	ubiquitin binding	4	2
GO:0044877	protein-containing complex binding	2	2
GO:0061133	endopeptidase activator activity	5	2
GO:0061134	peptidase regulator activity	3	2
GO:0061135	endopeptidase regulator activity	4	2
GO:0070628	proteasome binding	3	2
GO:0098772	molecular function regulator activity	1	2
GO:0140677	molecular function activator activity	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	102	106	PF00656	0.415
CLV_NRD_NRD_1	31	33	PF00675	0.238
CLV_PCSK_KEX2_1	31	33	PF00082	0.235
CLV_PCSK_PC1ET2_1	31	33	PF00082	0.289
CLV_PCSK_SKI1_1	135	139	PF00082	0.477
CLV_PCSK_SKI1_1	146	150	PF00082	0.479
CLV_PCSK_SKI1_1	78	82	PF00082	0.214
DEG_APCC_DBOX_1	77	85	PF00400	0.432
DEG_APCC_KENBOX_2	84	88	PF00400	0.351
DEG_SPOP_SBC_1	153	157	PF00917	0.545
DOC_MAPK_gen_1	124	131	PF00069	0.586
DOC_MAPK_gen_1	143	150	PF00069	0.511
DOC_MAPK_gen_1	31	38	PF00069	0.435
DOC_MAPK_gen_1	63	70	PF00069	0.481
DOC_PP4_FxxP_1	221	224	PF00568	0.542
DOC_PP4_FxxP_1	7	10	PF00568	0.535
DOC_PP4_MxPP_1	1	4	PF00568	0.571
DOC_USP7_MATH_1	139	143	PF00917	0.559
DOC_USP7_MATH_1	195	199	PF00917	0.437
DOC_USP7_MATH_1	50	54	PF00917	0.512
DOC_USP7_UBL2_3	100	104	PF12436	0.499
DOC_USP7_UBL2_3	145	149	PF12436	0.478
LIG_14-3-3_CanoR_1	124	130	PF00244	0.541
LIG_14-3-3_CanoR_1	63	69	PF00244	0.473
LIG_eIF4E_1	169	175	PF01652	0.402
LIG_EVH1_2	3	7	PF00568	0.538
LIG_FHA_1	126	132	PF00498	0.602
LIG_FHA_1	149	155	PF00498	0.492
LIG_FHA_1	199	205	PF00498	0.554
LIG_FHA_1	74	80	PF00498	0.446
LIG_LIR_Apic_2	219	224	PF02991	0.447
LIG_LIR_Nem_3	132	137	PF02991	0.489
LIG_LIR_Nem_3	212	217	PF02991	0.384
LIG_SH2_CRK	214	218	PF00017	0.469
LIG_SH2_STAT5	108	111	PF00017	0.275
LIG_SH2_STAT5	177	180	PF00017	0.599
LIG_SH2_STAT5	22	25	PF00017	0.543
LIG_SH2_STAT5	35	38	PF00017	0.507
LIG_SH2_STAT5	93	96	PF00017	0.503
LIG_SH3_3	189	195	PF00018	0.419
LIG_Sin3_3	200	207	PF02671	0.397
LIG_WRC_WIRS_1	35	40	PF05994	0.489
MOD_CK1_1	198	204	PF00069	0.493
MOD_CK1_1	37	43	PF00069	0.501
MOD_CK1_1	53	59	PF00069	0.555
MOD_CK1_1	69	75	PF00069	0.414
MOD_CK2_1	40	46	PF00069	0.455
MOD_CK2_1	48	54	PF00069	0.407
MOD_GlcNHglycan	40	43	PF01048	0.312
MOD_GlcNHglycan	54	58	PF01048	0.141
MOD_GSK3_1	137	144	PF00069	0.506
MOD_GSK3_1	148	155	PF00069	0.289
MOD_GSK3_1	34	41	PF00069	0.529
MOD_GSK3_1	69	76	PF00069	0.498
MOD_NEK2_1	154	159	PF00069	0.450
MOD_NEK2_1	20	25	PF00069	0.535
MOD_NEK2_1	38	43	PF00069	0.497
MOD_NEK2_2	34	39	PF00069	0.466
MOD_PIKK_1	195	201	PF00454	0.494
MOD_PIKK_1	69	75	PF00454	0.483
MOD_Plk_1	104	110	PF00069	0.287
MOD_Plk_4	104	110	PF00069	0.403
MOD_Plk_4	111	117	PF00069	0.461
MOD_Plk_4	154	160	PF00069	0.461
MOD_Plk_4	216	222	PF00069	0.546
MOD_Plk_4	34	40	PF00069	0.448
MOD_Plk_4	66	72	PF00069	0.509
MOD_SUMO_rev_2	132	137	PF00179	0.580
MOD_SUMO_rev_2	142	151	PF00179	0.609
MOD_SUMO_rev_2	99	106	PF00179	0.519
TRG_ENDOCYTIC_2	214	217	PF00928	0.443
TRG_ENDOCYTIC_2	35	38	PF00928	0.519

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1T1	Leptomonas seymouri	65%	99%
A0A0S4KJ38	Bodo saltans	33%	98%
A0A1X0P5X9	Trypanosomatidae	30%	89%
A0A1X0P6U7	Trypanosomatidae	40%	100%
A0A3Q8IIY1	Leishmania donovani	95%	100%
A0A3R7K022	Trypanosoma rangeli	31%	92%
A0A422MVV1	Trypanosoma rangeli	40%	100%
A4HMJ8	Leishmania braziliensis	87%	100%
A4IB77	Leishmania infantum	95%	100%
C9ZZD7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	88%
C9ZZK1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	97%
E9B654	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
Q556N5	Dictyostelium discoideum	23%	86%
Q9Y7Y6	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	23%	85%
V5ATF0	Trypanosoma cruzi	36%	100%