Protein phosphatase 1 regulatory subunit 22

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AEZ1_LEIMA

TriTrypDb:

LmjF.35.1690 , LMJLV39_350023300 , LMJSD75_350022500

Length:

378

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005929	cilium	4	12
GO:0042995	cell projection	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0110165	cellular anatomical entity	1	12
GO:0120025	plasma membrane bounded cell projection	3	12

Expansion

Sequence features

E9AEZ1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEZ1

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0019538	protein metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044238	primary metabolic process	2	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0019899	enzyme binding	3	2
GO:0019900	kinase binding	4	2
GO:0019901	protein kinase binding	5	2
GO:0003824	catalytic activity	1	2
GO:0016787	hydrolase activity	2	2
GO:0004175	endopeptidase activity	4	1
GO:0004197	cysteine-type endopeptidase activity	5	1
GO:0008233	peptidase activity	3	1
GO:0008234	cysteine-type peptidase activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	15	19	PF00656	0.736
CLV_NRD_NRD_1	186	188	PF00675	0.773
CLV_NRD_NRD_1	229	231	PF00675	0.652
CLV_NRD_NRD_1	292	294	PF00675	0.578
CLV_PCSK_FUR_1	306	310	PF00082	0.638
CLV_PCSK_KEX2_1	186	188	PF00082	0.772
CLV_PCSK_KEX2_1	229	231	PF00082	0.620
CLV_PCSK_KEX2_1	292	294	PF00082	0.558
CLV_PCSK_KEX2_1	308	310	PF00082	0.428
CLV_PCSK_PC1ET2_1	308	310	PF00082	0.579
CLV_PCSK_SKI1_1	292	296	PF00082	0.586
CLV_PCSK_SKI1_1	308	312	PF00082	0.442
CLV_PCSK_SKI1_1	55	59	PF00082	0.536
DEG_APCC_DBOX_1	158	166	PF00400	0.567
DEG_SPOP_SBC_1	11	15	PF00917	0.673
DOC_CYCLIN_RxL_1	287	300	PF00134	0.594
DOC_CYCLIN_RxL_1	304	314	PF00134	0.605
DOC_CYCLIN_RxL_1	33	44	PF00134	0.457
DOC_MAPK_gen_1	206	215	PF00069	0.390
DOC_MAPK_gen_1	304	313	PF00069	0.555
DOC_MAPK_MEF2A_6	273	281	PF00069	0.643
DOC_USP7_MATH_1	167	171	PF00917	0.557
DOC_USP7_MATH_1	241	245	PF00917	0.764
DOC_USP7_MATH_1	354	358	PF00917	0.574
DOC_USP7_UBL2_3	304	308	PF12436	0.630
DOC_USP7_UBL2_3	317	321	PF12436	0.538
DOC_WW_Pin1_4	135	140	PF00397	0.496
DOC_WW_Pin1_4	96	101	PF00397	0.489
LIG_14-3-3_CanoR_1	166	172	PF00244	0.580
LIG_Actin_WH2_2	326	343	PF00022	0.536
LIG_BIR_III_4	21	25	PF00653	0.643
LIG_BIR_III_4	250	254	PF00653	0.718
LIG_BIR_III_4	300	304	PF00653	0.541
LIG_eIF4E_1	42	48	PF01652	0.520
LIG_FHA_1	112	118	PF00498	0.552
LIG_FHA_1	195	201	PF00498	0.630
LIG_FHA_1	317	323	PF00498	0.565
LIG_FHA_1	32	38	PF00498	0.550
LIG_FHA_1	97	103	PF00498	0.547
LIG_FHA_2	13	19	PF00498	0.627
LIG_FHA_2	326	332	PF00498	0.545
LIG_FHA_2	47	53	PF00498	0.518
LIG_FHA_2	56	62	PF00498	0.458
LIG_HP1_1	167	171	PF01393	0.575
LIG_LIR_Gen_1	355	366	PF02991	0.583
LIG_LIR_Gen_1	41	48	PF02991	0.503
LIG_LIR_Nem_3	208	213	PF02991	0.526
LIG_LIR_Nem_3	355	361	PF02991	0.583
LIG_LIR_Nem_3	41	45	PF02991	0.504
LIG_NRBOX	290	296	PF00104	0.530
LIG_PCNA_yPIPBox_3	186	200	PF02747	0.472
LIG_PTB_Apo_2	36	43	PF02174	0.517
LIG_PTB_Phospho_1	36	42	PF10480	0.515
LIG_RPA_C_Fungi	240	252	PF08784	0.691
LIG_SH2_CRK	42	46	PF00017	0.529
LIG_SH2_STAT5	98	101	PF00017	0.574
LIG_SUMO_SIM_par_1	58	63	PF11976	0.440
LIG_TYR_ITIM	40	45	PF00017	0.509
MOD_CDK_SPK_2	96	101	PF00069	0.468
MOD_CK1_1	13	19	PF00069	0.713
MOD_CK1_1	158	164	PF00069	0.528
MOD_CK1_1	255	261	PF00069	0.574
MOD_CK1_1	262	268	PF00069	0.613
MOD_CK1_1	339	345	PF00069	0.550
MOD_CK1_1	41	47	PF00069	0.483
MOD_CK1_1	63	69	PF00069	0.346
MOD_CK2_1	186	192	PF00069	0.738
MOD_CK2_1	325	331	PF00069	0.564
MOD_CK2_1	46	52	PF00069	0.539
MOD_Cter_Amidation	184	187	PF01082	0.760
MOD_GlcNHglycan	239	242	PF01048	0.717
MOD_GlcNHglycan	254	257	PF01048	0.591
MOD_GlcNHglycan	29	32	PF01048	0.639
MOD_GlcNHglycan	372	375	PF01048	0.591
MOD_GSK3_1	181	188	PF00069	0.684
MOD_GSK3_1	237	244	PF00069	0.597
MOD_GSK3_1	252	259	PF00069	0.605
MOD_GSK3_1	27	34	PF00069	0.584
MOD_GSK3_1	361	368	PF00069	0.554
MOD_GSK3_1	46	53	PF00069	0.436
MOD_GSK3_1	65	72	PF00069	0.331
MOD_GSK3_1	7	14	PF00069	0.625
MOD_N-GLC_1	130	135	PF02516	0.529
MOD_N-GLC_1	370	375	PF02516	0.439
MOD_N-GLC_1	38	43	PF02516	0.474
MOD_NEK2_1	12	17	PF00069	0.734
MOD_NEK2_1	144	149	PF00069	0.545
MOD_NEK2_1	181	186	PF00069	0.691
MOD_NEK2_1	263	268	PF00069	0.708
MOD_NEK2_1	347	352	PF00069	0.585
MOD_NEK2_1	365	370	PF00069	0.546
MOD_NEK2_1	38	43	PF00069	0.401
MOD_NEK2_1	60	65	PF00069	0.411
MOD_NEK2_2	167	172	PF00069	0.524
MOD_NEK2_2	50	55	PF00069	0.546
MOD_NMyristoyl	1	7	PF02799	0.410
MOD_PIKK_1	194	200	PF00454	0.606
MOD_PIKK_1	213	219	PF00454	0.361
MOD_PIKK_1	264	270	PF00454	0.723
MOD_PIKK_1	295	301	PF00454	0.599
MOD_PIKK_1	65	71	PF00454	0.531
MOD_PKA_1	186	192	PF00069	0.752
MOD_PKA_2	158	164	PF00069	0.539
MOD_PKA_2	185	191	PF00069	0.767
MOD_PKA_2	205	211	PF00069	0.575
MOD_PKA_2	80	86	PF00069	0.524
MOD_Plk_1	130	136	PF00069	0.558
MOD_Plk_1	354	360	PF00069	0.532
MOD_Plk_1	38	44	PF00069	0.442
MOD_Plk_2-3	130	136	PF00069	0.543
MOD_Plk_4	259	265	PF00069	0.700
MOD_Plk_4	361	367	PF00069	0.516
MOD_Plk_4	55	61	PF00069	0.488
MOD_Plk_4	75	81	PF00069	0.404
MOD_ProDKin_1	135	141	PF00069	0.496
MOD_ProDKin_1	96	102	PF00069	0.482
MOD_SUMO_rev_2	298	305	PF00179	0.567
TRG_DiLeu_BaEn_1	290	295	PF01217	0.504
TRG_DiLeu_BaLyEn_6	33	38	PF01217	0.570
TRG_DiLeu_LyEn_5	290	295	PF01217	0.526
TRG_ENDOCYTIC_2	42	45	PF00928	0.508
TRG_ER_diArg_1	291	293	PF00400	0.593
TRG_Pf-PMV_PEXEL_1	309	314	PF00026	0.517

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4E0	Leptomonas seymouri	52%	88%
A0A0S4JD31	Bodo saltans	27%	74%
A0A0S4KH47	Bodo saltans	28%	99%
A0A1X0P5U4	Trypanosomatidae	35%	100%
A0A3Q8IF21	Leishmania donovani	88%	86%
A0A422NQ71	Trypanosoma rangeli	38%	100%
A4HMJ0	Leishmania braziliensis	77%	90%
A4IB70	Leishmania infantum	88%	100%
C9ZZH9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
E9B647	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	76%