RRM domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA binding protein, putative

Species:

Leishmania major

UniProt:

E9AEW3_LEIMA

TriTrypDb:

LmjF.35.1400 , LMJLV39_350020400 , LMJSD75_350019600

Length:

232

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AEW3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEW3

Function

Biological processes
Term	Name	Level	Count
GO:0000447	endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	10	2
GO:0000469	cleavage involved in rRNA processing	7	2
GO:0000472	endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	10	2
GO:0000478	endonucleolytic cleavage involved in rRNA processing	8	2
GO:0000479	endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	9	2
GO:0000480	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	10	2
GO:0000966	RNA 5'-end processing	7	2
GO:0000967	rRNA 5'-end processing	9	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006364	rRNA processing	8	2
GO:0006396	RNA processing	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0016070	RNA metabolic process	5	2
GO:0016072	rRNA metabolic process	7	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0034462	small-subunit processome assembly	7	2
GO:0034470	ncRNA processing	7	2
GO:0034471	ncRNA 5'-end processing	8	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0036260	RNA capping	7	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043933	protein-containing complex organization	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0065003	protein-containing complex assembly	5	2
GO:0071704	organic substance metabolic process	2	2
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	2
GO:0090501	RNA phosphodiester bond hydrolysis	6	2
GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic	7	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	8
GO:0003723	RNA binding	4	7
GO:0005488	binding	1	8
GO:0097159	organic cyclic compound binding	2	8
GO:1901363	heterocyclic compound binding	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	170	174	PF00656	0.725
CLV_C14_Caspase3-7	74	78	PF00656	0.562
CLV_NRD_NRD_1	124	126	PF00675	0.508
CLV_NRD_NRD_1	127	129	PF00675	0.502
CLV_NRD_NRD_1	135	137	PF00675	0.482
CLV_NRD_NRD_1	146	148	PF00675	0.450
CLV_NRD_NRD_1	155	157	PF00675	0.383
CLV_NRD_NRD_1	160	162	PF00675	0.490
CLV_NRD_NRD_1	200	202	PF00675	0.697
CLV_NRD_NRD_1	229	231	PF00675	0.771
CLV_NRD_NRD_1	42	44	PF00675	0.502
CLV_NRD_NRD_1	54	56	PF00675	0.455
CLV_NRD_NRD_1	96	98	PF00675	0.492
CLV_PCSK_FUR_1	125	129	PF00082	0.485
CLV_PCSK_FUR_1	94	98	PF00082	0.523
CLV_PCSK_KEX2_1	123	125	PF00082	0.512
CLV_PCSK_KEX2_1	127	129	PF00082	0.496
CLV_PCSK_KEX2_1	135	137	PF00082	0.471
CLV_PCSK_KEX2_1	146	148	PF00082	0.513
CLV_PCSK_KEX2_1	159	161	PF00082	0.488
CLV_PCSK_KEX2_1	200	202	PF00082	0.614
CLV_PCSK_KEX2_1	227	229	PF00082	0.789
CLV_PCSK_KEX2_1	42	44	PF00082	0.502
CLV_PCSK_KEX2_1	54	56	PF00082	0.455
CLV_PCSK_KEX2_1	93	95	PF00082	0.531
CLV_PCSK_KEX2_1	96	98	PF00082	0.491
CLV_PCSK_PC1ET2_1	123	125	PF00082	0.618
CLV_PCSK_PC1ET2_1	159	161	PF00082	0.649
CLV_PCSK_PC1ET2_1	227	229	PF00082	0.789
CLV_PCSK_PC1ET2_1	93	95	PF00082	0.496
CLV_PCSK_PC7_1	156	162	PF00082	0.692
CLV_PCSK_SKI1_1	14	18	PF00082	0.459
CLV_PCSK_SKI1_1	156	160	PF00082	0.609
CLV_PCSK_SKI1_1	205	209	PF00082	0.744
CLV_PCSK_SKI1_1	224	228	PF00082	0.676
CLV_PCSK_SKI1_1	42	46	PF00082	0.552
DOC_MAPK_gen_1	24	33	PF00069	0.569
DOC_MAPK_gen_1	42	49	PF00069	0.288
DOC_MAPK_MEF2A_6	27	35	PF00069	0.562
DOC_MAPK_MEF2A_6	54	63	PF00069	0.564
DOC_MAPK_RevD_3	81	94	PF00069	0.350
DOC_PP1_RVXF_1	30	36	PF00149	0.567
DOC_USP7_MATH_1	137	141	PF00917	0.546
DOC_USP7_MATH_1	169	173	PF00917	0.705
DOC_USP7_MATH_1	186	190	PF00917	0.714
DOC_USP7_MATH_1	203	207	PF00917	0.515
DOC_USP7_MATH_1	215	219	PF00917	0.631
DOC_USP7_UBL2_3	205	209	PF12436	0.677
LIG_14-3-3_CanoR_1	200	207	PF00244	0.545
LIG_AP2alpha_1	107	111	PF02296	0.468
LIG_BIR_II_1	1	5	PF00653	0.700
LIG_BIR_III_4	175	179	PF00653	0.680
LIG_deltaCOP1_diTrp_1	67	72	PF00928	0.530
LIG_FHA_1	110	116	PF00498	0.325
LIG_FHA_1	221	227	PF00498	0.613
LIG_LIR_Apic_2	5	11	PF02991	0.497
LIG_LIR_Gen_1	112	120	PF02991	0.417
LIG_LIR_Gen_1	206	215	PF02991	0.545
LIG_LIR_Gen_1	67	75	PF02991	0.506
LIG_LIR_Nem_3	112	116	PF02991	0.402
LIG_LIR_Nem_3	206	210	PF02991	0.546
LIG_LIR_Nem_3	67	72	PF02991	0.497
LIG_Pex14_1	103	107	PF04695	0.390
LIG_Pex14_2	107	111	PF04695	0.487
LIG_SH2_CRK	8	12	PF00017	0.586
LIG_SH2_GRB2like	18	21	PF00017	0.574
LIG_SH2_NCK_1	8	12	PF00017	0.586
LIG_SH2_SRC	8	11	PF00017	0.454
LIG_SH2_STAT5	18	21	PF00017	0.502
LIG_SH3_3	53	59	PF00018	0.538
LIG_UBA3_1	60	66	PF00899	0.598
MOD_CK1_1	181	187	PF00069	0.680
MOD_CK1_1	189	195	PF00069	0.747
MOD_CK2_1	137	143	PF00069	0.624
MOD_Cter_Amidation	121	124	PF01082	0.526
MOD_Cter_Amidation	198	201	PF01082	0.663
MOD_GlcNHglycan	188	191	PF01048	0.712
MOD_GlcNHglycan	213	216	PF01048	0.751
MOD_GlcNHglycan	73	76	PF01048	0.531
MOD_GSK3_1	137	144	PF00069	0.452
MOD_GSK3_1	195	202	PF00069	0.657
MOD_GSK3_1	211	218	PF00069	0.702
MOD_N-GLC_1	141	146	PF02516	0.400
MOD_NEK2_1	141	146	PF00069	0.462
MOD_NEK2_1	70	75	PF00069	0.471
MOD_PIKK_1	9	15	PF00454	0.492
MOD_PKA_2	199	205	PF00069	0.543
MOD_PKB_1	193	201	PF00069	0.671
MOD_Plk_1	137	143	PF00069	0.624
MOD_Plk_2-3	109	115	PF00069	0.495
TRG_DiLeu_BaLyEn_6	56	61	PF01217	0.566
TRG_ENDOCYTIC_2	145	148	PF00928	0.646
TRG_ENDOCYTIC_2	38	41	PF00928	0.534
TRG_ENDOCYTIC_2	99	102	PF00928	0.506
TRG_ER_diArg_1	124	127	PF00400	0.479
TRG_ER_diArg_1	145	147	PF00400	0.539
TRG_ER_diArg_1	160	163	PF00400	0.532
TRG_ER_diArg_1	228	230	PF00400	0.699
TRG_ER_diArg_1	41	43	PF00400	0.421
TRG_ER_diArg_1	54	56	PF00400	0.515
TRG_ER_diArg_1	94	97	PF00400	0.486
TRG_ER_FFAT_2	67	77	PF00635	0.547
TRG_NLS_Bipartite_1	146	163	PF00514	0.596
TRG_NLS_MonoExtC_3	122	127	PF00514	0.540
TRG_NLS_MonoExtC_3	155	160	PF00514	0.607
TRG_NLS_MonoExtC_3	223	228	PF00514	0.771
TRG_NLS_MonoExtC_3	50	55	PF00514	0.651
TRG_NLS_MonoExtC_3	92	97	PF00514	0.476
TRG_NLS_MonoExtN_4	156	163	PF00514	0.634
TRG_NLS_MonoExtN_4	222	228	PF00514	0.743
TRG_NLS_MonoExtN_4	48	55	PF00514	0.641

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NJ36	Trypanosomatidae	50%	85%
A0A3R7N8I1	Trypanosoma rangeli	56%	100%
A0A3S7X912	Leishmania donovani	95%	100%
A3LVD5	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	26%	67%
A4HMG2	Leishmania braziliensis	81%	100%
A4IB33	Leishmania infantum	95%	100%
E9B619	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
P53743	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	73%
Q0UM32	Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)	28%	71%
Q2GZQ4	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	28%	70%
Q54BL2	Dictyostelium discoideum	25%	78%
Q59YL9	Candida albicans (strain SC5314 / ATCC MYA-2876)	25%	72%
Q6BSS5	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	24%	77%
Q6CSP8	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	26%	79%
Q75DA3	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	26%	74%
Q7S8W7	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	28%	68%
Q9QYL7	Mus musculus	26%	86%