Thioredoxin-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

monothiol glutaredoxin, putative

Species:

Leishmania major

UniProt:

E9AEU8_LEIMA

TriTrypDb:

LmjF.35.1250 , LMJLV39_350018900 , LMJSD75_350018100

Length:

228

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	2
GO:0005829	cytosol	2	2
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AEU8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEU8

Function

Biological processes
Term	Name	Level	Count
GO:0006873	intracellular monoatomic ion homeostasis	4	2
GO:0006875	obsolete intracellular metal ion homeostasis	6	2
GO:0006879	intracellular iron ion homeostasis	8	2
GO:0009987	cellular process	1	2
GO:0019725	cellular homeostasis	2	2
GO:0030003	intracellular monoatomic cation homeostasis	5	2
GO:0042592	homeostatic process	3	2
GO:0045454	cell redox homeostasis	3	2
GO:0046916	obsolete intracellular transition metal ion homeostasis	7	2
GO:0048878	chemical homeostasis	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050801	monoatomic ion homeostasis	5	2
GO:0055065	obsolete metal ion homeostasis	7	2
GO:0055072	obsolete iron ion homeostasis	9	2
GO:0055076	obsolete transition metal ion homeostasis	8	2
GO:0055080	monoatomic cation homeostasis	6	2
GO:0055082	intracellular chemical homeostasis	3	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0098771	inorganic ion homeostasis	6	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004362	glutathione-disulfide reductase (NADPH) activity	2	2
GO:0005488	binding	1	10
GO:0015035	protein-disulfide reductase activity	3	11
GO:0015036	disulfide oxidoreductase activity	4	11
GO:0015038	glutathione disulfide oxidoreductase activity	5	2
GO:0016209	antioxidant activity	1	2
GO:0016491	oxidoreductase activity	2	11
GO:0016667	oxidoreductase activity, acting on a sulfur group of donors	3	11
GO:0016668	oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor	4	2
GO:0043167	ion binding	2	10
GO:0043169	cation binding	3	10
GO:0046872	metal ion binding	4	10
GO:0047134	protein-disulfide reductase (NAD(P)) activity	4	2
GO:0051536	iron-sulfur cluster binding	3	10
GO:0051540	metal cluster binding	2	10
GO:0097573	obsolete glutathione oxidoreductase activity	4	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	48	52	PF00656	0.459
CLV_NRD_NRD_1	90	92	PF00675	0.516
CLV_PCSK_KEX2_1	139	141	PF00082	0.506
CLV_PCSK_KEX2_1	90	92	PF00082	0.568
CLV_PCSK_PC1ET2_1	139	141	PF00082	0.506
DEG_Nend_Nbox_1	1	3	PF02207	0.521
DOC_CKS1_1	81	86	PF01111	0.513
DOC_CYCLIN_yCln2_LP_2	169	175	PF00134	0.426
DOC_MAPK_gen_1	139	147	PF00069	0.280
DOC_MAPK_gen_1	14	23	PF00069	0.430
DOC_MAPK_HePTP_8	13	25	PF00069	0.459
DOC_MAPK_MEF2A_6	16	25	PF00069	0.394
DOC_PP2B_LxvP_1	169	172	PF13499	0.426
DOC_PP4_FxxP_1	81	84	PF00568	0.479
DOC_USP7_MATH_1	17	21	PF00917	0.459
DOC_USP7_UBL2_3	223	227	PF12436	0.431
DOC_USP7_UBL2_3	3	7	PF12436	0.459
DOC_WW_Pin1_4	147	152	PF00397	0.302
DOC_WW_Pin1_4	80	85	PF00397	0.442
LIG_Actin_WH2_2	209	225	PF00022	0.395
LIG_BRCT_BRCA1_1	73	77	PF00533	0.426
LIG_eIF4E_1	201	207	PF01652	0.333
LIG_eIF4E_1	80	86	PF01652	0.549
LIG_FHA_1	108	114	PF00498	0.535
LIG_FHA_1	157	163	PF00498	0.314
LIG_FHA_1	81	87	PF00498	0.562
LIG_FHA_2	127	133	PF00498	0.525
LIG_FHA_2	28	34	PF00498	0.413
LIG_LIR_Apic_2	28	34	PF02991	0.316
LIG_LIR_Gen_1	126	134	PF02991	0.416
LIG_LIR_Gen_1	8	17	PF02991	0.348
LIG_LIR_LC3C_4	20	25	PF02991	0.459
LIG_LIR_Nem_3	126	130	PF02991	0.422
LIG_LIR_Nem_3	131	136	PF02991	0.393
LIG_LIR_Nem_3	196	200	PF02991	0.347
LIG_LIR_Nem_3	74	80	PF02991	0.375
LIG_LIR_Nem_3	8	13	PF02991	0.336
LIG_LYPXL_yS_3	197	200	PF13949	0.426
LIG_Pex14_2	27	31	PF04695	0.330
LIG_Pex14_2	77	81	PF04695	0.387
LIG_SH2_GRB2like	191	194	PF00017	0.426
LIG_SH2_SRC	10	13	PF00017	0.403
LIG_SH2_SRC	201	204	PF00017	0.314
LIG_SH2_STAT5	133	136	PF00017	0.378
LIG_SH2_STAT5	201	204	PF00017	0.322
LIG_SH2_STAT5	80	83	PF00017	0.433
LIG_SH3_3	148	154	PF00018	0.313
LIG_SH3_3	192	198	PF00018	0.300
MOD_CDK_SPxK_1	147	153	PF00069	0.314
MOD_CK2_1	2	8	PF00069	0.337
MOD_CK2_1	27	33	PF00069	0.368
MOD_GlcNHglycan	27	30	PF01048	0.303
MOD_GSK3_1	145	152	PF00069	0.341
MOD_NEK2_1	145	150	PF00069	0.387
MOD_NEK2_1	2	7	PF00069	0.459
MOD_NEK2_1	222	227	PF00069	0.491
MOD_NEK2_1	27	32	PF00069	0.368
MOD_Plk_1	17	23	PF00069	0.303
MOD_Plk_2-3	126	132	PF00069	0.540
MOD_Plk_4	109	115	PF00069	0.471
MOD_ProDKin_1	147	153	PF00069	0.302
MOD_ProDKin_1	80	86	PF00069	0.460
MOD_SUMO_rev_2	215	225	PF00179	0.416
TRG_DiLeu_BaEn_2	72	78	PF01217	0.335
TRG_ENDOCYTIC_2	10	13	PF00928	0.357
TRG_ENDOCYTIC_2	175	178	PF00928	0.300
TRG_ENDOCYTIC_2	197	200	PF00928	0.426
TRG_ER_diArg_1	90	92	PF00400	0.568
TRG_Pf-PMV_PEXEL_1	214	218	PF00026	0.507

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKK7	Leptomonas seymouri	78%	100%
A0A0S4JWQ4	Bodo saltans	53%	100%
A0A1X0NHB9	Trypanosomatidae	59%	100%
A0A3Q8IP85	Leishmania donovani	98%	100%
A0A422MXN1	Trypanosoma rangeli	57%	100%
A4HME7	Leishmania braziliensis	90%	100%
A4IB19	Leishmania infantum	97%	100%
C9ZNL5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	100%
E9B604	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
O74790	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	93%
O76003	Homo sapiens	33%	68%
P32642	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	21%	93%
Q03835	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	91%
Q28ID3	Xenopus tropicalis	30%	70%
Q58DA7	Bos taurus	32%	68%
Q5XJ54	Danio rerio	31%	70%
Q86H62	Dictyostelium discoideum	29%	95%
Q9CQM9	Mus musculus	32%	68%
Q9JLZ1	Rattus norvegicus	32%	68%