Putative NADH-dependent fumarate reductase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NADH-dependent fumarate reductase, putative

Species:

Leishmania major

UniProt:

E9AEU2_LEIMA

TriTrypDb:

LmjF.35.1190 , LMJLV39_350018200 , LMJSD75_350017400

Length:

495

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005777	peroxisome	6	2
GO:0005815	microtubule organizing center	2	2
GO:0020015	glycosome	7	2
GO:0036064	ciliary basal body	3	2
GO:0042579	microbody	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AEU2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEU2

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0010181	FMN binding	4	11
GO:0016156	fumarate reductase (NADH) activity	5	5
GO:0016491	oxidoreductase activity	2	11
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	6
GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor	4	5
GO:0032553	ribonucleotide binding	3	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0000104	succinate dehydrogenase activity	4	1
GO:0008177	succinate dehydrogenase (ubiquinone) activity	5	1
GO:0016635	oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	133	137	PF00656	0.303
CLV_NRD_NRD_1	124	126	PF00675	0.294
CLV_NRD_NRD_1	175	177	PF00675	0.179
CLV_NRD_NRD_1	331	333	PF00675	0.330
CLV_PCSK_KEX2_1	126	128	PF00082	0.294
CLV_PCSK_KEX2_1	331	333	PF00082	0.330
CLV_PCSK_PC1ET2_1	126	128	PF00082	0.294
CLV_PCSK_SKI1_1	280	284	PF00082	0.361
CLV_PCSK_SKI1_1	403	407	PF00082	0.298
CLV_Separin_Metazoa	91	95	PF03568	0.354
DOC_CDC14_PxL_1	445	453	PF14671	0.157
DOC_CKS1_1	420	425	PF01111	0.294
DOC_CKS1_1	490	495	PF01111	0.340
DOC_MAPK_DCC_7	134	144	PF00069	0.185
DOC_MAPK_gen_1	176	183	PF00069	0.302
DOC_MAPK_gen_1	249	258	PF00069	0.385
DOC_MAPK_MEF2A_6	249	258	PF00069	0.385
DOC_PP4_FxxP_1	110	113	PF00568	0.385
DOC_PP4_FxxP_1	283	286	PF00568	0.270
DOC_USP7_MATH_1	19	23	PF00917	0.339
DOC_USP7_UBL2_3	396	400	PF12436	0.223
DOC_WW_Pin1_4	135	140	PF00397	0.294
DOC_WW_Pin1_4	217	222	PF00397	0.330
DOC_WW_Pin1_4	419	424	PF00397	0.286
DOC_WW_Pin1_4	432	437	PF00397	0.270
DOC_WW_Pin1_4	489	494	PF00397	0.382
LIG_14-3-3_CanoR_1	169	174	PF00244	0.403
LIG_14-3-3_CanoR_1	311	319	PF00244	0.385
LIG_14-3-3_CanoR_1	348	352	PF00244	0.245
LIG_Actin_WH2_2	366	384	PF00022	0.157
LIG_APCC_ABBA_1	358	363	PF00400	0.330
LIG_APCC_ABBAyCdc20_2	357	363	PF00400	0.330
LIG_BIR_II_1	1	5	PF00653	0.628
LIG_BIR_III_4	344	348	PF00653	0.223
LIG_BRCT_BRCA1_1	349	353	PF00533	0.330
LIG_Clathr_ClatBox_1	258	262	PF01394	0.294
LIG_deltaCOP1_diTrp_1	105	110	PF00928	0.330
LIG_FHA_1	159	165	PF00498	0.385
LIG_FHA_1	198	204	PF00498	0.325
LIG_FHA_1	236	242	PF00498	0.459
LIG_FHA_1	267	273	PF00498	0.270
LIG_FHA_1	279	285	PF00498	0.270
LIG_FHA_1	420	426	PF00498	0.279
LIG_FHA_1	456	462	PF00498	0.304
LIG_FHA_1	50	56	PF00498	0.236
LIG_FHA_2	382	388	PF00498	0.396
LIG_FHA_2	399	405	PF00498	0.157
LIG_FHA_2	93	99	PF00498	0.350
LIG_LIR_Apic_2	108	113	PF02991	0.386
LIG_LIR_Apic_2	281	286	PF02991	0.296
LIG_LIR_Nem_3	386	391	PF02991	0.330
LIG_LIR_Nem_3	404	409	PF02991	0.294
LIG_LRP6_Inhibitor_1	149	155	PF00058	0.354
LIG_PCNA_yPIPBox_3	167	177	PF02747	0.324
LIG_PCNA_yPIPBox_3	466	477	PF02747	0.272
LIG_Pex14_1	106	110	PF04695	0.385
LIG_SH2_CRK	335	339	PF00017	0.385
LIG_SH2_NCK_1	388	392	PF00017	0.330
LIG_SH2_PTP2	417	420	PF00017	0.330
LIG_SH2_STAP1	333	337	PF00017	0.245
LIG_SH2_STAT3	189	192	PF00017	0.354
LIG_SH2_STAT3	228	231	PF00017	0.385
LIG_SH2_STAT5	153	156	PF00017	0.385
LIG_SH2_STAT5	335	338	PF00017	0.396
LIG_SH2_STAT5	417	420	PF00017	0.281
LIG_SH2_STAT5	427	430	PF00017	0.252
LIG_SH3_3	129	135	PF00018	0.411
LIG_SH3_3	271	277	PF00018	0.270
LIG_SH3_4	396	403	PF00018	0.223
LIG_SUMO_SIM_anti_2	178	185	PF11976	0.177
LIG_SUMO_SIM_anti_2	34	39	PF11976	0.294
LIG_SUMO_SIM_par_1	10	16	PF11976	0.352
LIG_SUMO_SIM_par_1	159	166	PF11976	0.245
LIG_SUMO_SIM_par_1	34	39	PF11976	0.261
LIG_TRAF2_1	195	198	PF00917	0.252
LIG_UBA3_1	170	177	PF00899	0.354
LIG_WRC_WIRS_1	107	112	PF05994	0.385
MOD_CK1_1	234	240	PF00069	0.317
MOD_CK1_1	312	318	PF00069	0.294
MOD_CK1_1	97	103	PF00069	0.292
MOD_CK2_1	176	182	PF00069	0.371
MOD_CK2_1	192	198	PF00069	0.217
MOD_CK2_1	381	387	PF00069	0.334
MOD_CK2_1	398	404	PF00069	0.223
MOD_CK2_1	468	474	PF00069	0.294
MOD_GlcNHglycan	15	18	PF01048	0.260
MOD_GlcNHglycan	158	161	PF01048	0.348
MOD_GlcNHglycan	194	197	PF01048	0.358
MOD_GlcNHglycan	21	24	PF01048	0.233
MOD_GlcNHglycan	291	294	PF01048	0.316
MOD_GlcNHglycan	392	395	PF01048	0.324
MOD_GlcNHglycan	47	50	PF01048	0.250
MOD_GlcNHglycan	52	55	PF01048	0.262
MOD_GlcNHglycan	70	73	PF01048	0.413
MOD_GlcNHglycan	81	84	PF01048	0.306
MOD_GlcNHglycan	98	102	PF01048	0.186
MOD_GSK3_1	188	195	PF00069	0.201
MOD_GSK3_1	209	216	PF00069	0.319
MOD_GSK3_1	230	237	PF00069	0.296
MOD_GSK3_1	377	384	PF00069	0.270
MOD_GSK3_1	421	428	PF00069	0.330
MOD_GSK3_1	45	52	PF00069	0.236
MOD_N-GLC_1	156	161	PF02516	0.427
MOD_NEK2_1	158	163	PF00069	0.397
MOD_NEK2_1	289	294	PF00069	0.298
MOD_NEK2_1	346	351	PF00069	0.245
MOD_NEK2_1	381	386	PF00069	0.274
MOD_NEK2_1	92	97	PF00069	0.348
MOD_NEK2_2	377	382	PF00069	0.205
MOD_PIKK_1	115	121	PF00454	0.294
MOD_PIKK_1	188	194	PF00454	0.354
MOD_PIKK_1	312	318	PF00454	0.385
MOD_PIKK_1	409	415	PF00454	0.330
MOD_PKA_1	176	182	PF00069	0.168
MOD_PKA_2	289	295	PF00069	0.270
MOD_PKA_2	347	353	PF00069	0.330
MOD_Plk_1	377	383	PF00069	0.330
MOD_Plk_4	158	164	PF00069	0.266
MOD_Plk_4	220	226	PF00069	0.270
MOD_Plk_4	278	284	PF00069	0.325
MOD_Plk_4	468	474	PF00069	0.270
MOD_Plk_4	76	82	PF00069	0.245
MOD_ProDKin_1	135	141	PF00069	0.294
MOD_ProDKin_1	217	223	PF00069	0.330
MOD_ProDKin_1	419	425	PF00069	0.286
MOD_ProDKin_1	432	438	PF00069	0.270
MOD_ProDKin_1	489	495	PF00069	0.398
TRG_DiLeu_BaEn_2	75	81	PF01217	0.245
TRG_DiLeu_BaLyEn_6	166	171	PF01217	0.354
TRG_ENDOCYTIC_2	335	338	PF00928	0.404
TRG_ENDOCYTIC_2	388	391	PF00928	0.330
TRG_ENDOCYTIC_2	417	420	PF00928	0.331
TRG_ER_diArg_1	331	333	PF00400	0.330
TRG_Pf-PMV_PEXEL_1	94	98	PF00026	0.385

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5C0	Leptomonas seymouri	78%	82%
A0A0S4IHN0	Bodo saltans	59%	100%
A0A0S4KND3	Bodo saltans	39%	95%
A0A1S3YEG8	Nicotiana tabacum	26%	77%
A0A1S4BJT3	Nicotiana tabacum	26%	76%
A0A1X0NPF8	Trypanosomatidae	36%	73%
A0A3S7WPX2	Leishmania donovani	36%	74%
A0A3S7X915	Leishmania donovani	96%	100%
A0A422MX37	Trypanosoma rangeli	63%	75%
A0R4S9	Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)	24%	87%
A4HME0	Leishmania braziliensis	80%	96%
A4HME1	Leishmania braziliensis	76%	91%
A4HTA6	Leishmania infantum	35%	87%
A4IB13	Leishmania infantum	96%	100%
B2GCE0	Limosilactobacillus fermentum (strain NBRC 3956 / LMG 18251)	29%	80%
C8WLE3	Eggerthella lenta (strain ATCC 25559 / DSM 2243 / CCUG 17323 / JCM 9979 / KCTC 3265 / NCTC 11813 / VPI 0255 / 1899 B)	30%	82%
C8WLM1	Eggerthella lenta (strain ATCC 25559 / DSM 2243 / CCUG 17323 / JCM 9979 / KCTC 3265 / NCTC 11813 / VPI 0255 / 1899 B)	24%	88%
D9PU00	Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg)	27%	90%
E9AL95	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	74%
E9B5Z8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
G4V4G6	Serratia sp. (strain ATCC 39006)	28%	84%
O13755	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	38%	96%
O57765	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	27%	100%
P00363	Escherichia coli (strain K12)	28%	82%
P08065	Bacillus subtilis (strain 168)	27%	84%
P0AC41	Escherichia coli (strain K12)	28%	84%
P0AC42	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	28%	84%
P0AC43	Escherichia coli O157:H7	28%	84%
P0C278	Shewanella frigidimarina	37%	87%
P10902	Escherichia coli (strain K12)	27%	92%
P17412	Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)	26%	75%
P20922	Proteus vulgaris	28%	83%
P21375	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	36%	99%
P32614	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	38%	100%
P44894	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	29%	83%
P64175	Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)	27%	85%
P71864	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	25%	88%
P74562	Synechocystis sp. (strain PCC 6803 / Kazusa)	29%	90%
P83223	Shewanella oneidensis (strain MR-1)	33%	83%
P9WN90	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	27%	85%
P9WN91	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	27%	85%
Q07WU7	Shewanella frigidimarina (strain NCIMB 400)	37%	83%
Q49617	Mycobacterium leprae (strain TN)	28%	94%
Q4QIK9	Leishmania major	36%	74%
Q4WR24	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	25%	83%
Q51363	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	29%	92%
Q60356	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	30%	92%
Q6Z836	Oryza sativa subsp. japonica	26%	77%
Q7D5C1	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	25%	87%
Q7M827	Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)	26%	81%
Q8CVD0	Shewanella oneidensis (strain MR-1)	34%	85%
Q8U8J4	Agrobacterium fabrum (strain C58 / ATCC 33970)	33%	95%
Q8XA23	Escherichia coli O157:H7	27%	92%
Q8XWM7	Ralstonia solanacearum (strain GMI1000)	28%	93%
Q8YXJ6	Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)	30%	86%
Q8Z4K0	Salmonella typhi	26%	92%
Q8ZD80	Yersinia pestis	25%	93%
Q8ZMX9	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	26%	92%
Q8ZQU3	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	29%	84%
Q92R32	Rhizobium meliloti (strain 1021)	28%	93%
Q94AY1	Arabidopsis thaliana	26%	76%
Q97K95	Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)	26%	100%
Q9A4C3	Caulobacter vibrioides (strain ATCC 19089 / CB15)	28%	97%
Q9HNZ0	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	27%	97%
Q9KPA4	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)	26%	93%
Q9PC57	Xylella fastidiosa (strain 9a5c)	28%	97%
Q9V2R0	Pyrococcus abyssi (strain GE5 / Orsay)	28%	100%
Q9X8N8	Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)	27%	85%
Q9Z4P0	Shewanella frigidimarina (strain NCIMB 400)	36%	84%
V3TQ67	Serratia sp. (strain ATCC 39006)	27%	83%