Putative NADH-dependent fumarate reductase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NADH-dependent fumarate reductase, putative

Species:

Leishmania major

UniProt:

E9AEU1_LEIMA

TriTrypDb:

LmjF.35.1180 , LMJLV39_350018100 , LMJSD75_350017300

Length:

1147

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005777	peroxisome	6	2
GO:0005815	microtubule organizing center	2	2
GO:0020015	glycosome	7	2
GO:0036064	ciliary basal body	3	2
GO:0042579	microbody	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AEU1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEU1

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	20
GO:0008152	metabolic process	1	20
GO:0017013	protein flavinylation	5	20
GO:0019538	protein metabolic process	3	20
GO:0036211	protein modification process	4	20
GO:0043170	macromolecule metabolic process	3	20
GO:0043412	macromolecule modification	4	20
GO:0044238	primary metabolic process	2	20
GO:0071704	organic substance metabolic process	2	20
GO:1901564	organonitrogen compound metabolic process	3	20

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	19
GO:0003824	catalytic activity	1	20
GO:0005488	binding	1	20
GO:0010181	FMN binding	4	19
GO:0016491	oxidoreductase activity	2	20
GO:0016740	transferase activity	2	20
GO:0032553	ribonucleotide binding	3	19
GO:0036094	small molecule binding	2	19
GO:0043167	ion binding	2	20
GO:0043168	anion binding	3	19
GO:0043169	cation binding	3	20
GO:0046872	metal ion binding	4	20
GO:0097159	organic cyclic compound binding	2	19
GO:0097367	carbohydrate derivative binding	2	19
GO:1901265	nucleoside phosphate binding	3	19
GO:1901363	heterocyclic compound binding	2	19
GO:0016156	fumarate reductase (NADH) activity	5	1
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	1
GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	511	515	PF00656	0.330
CLV_NRD_NRD_1	1006	1008	PF00675	0.367
CLV_NRD_NRD_1	182	184	PF00675	0.389
CLV_NRD_NRD_1	338	340	PF00675	0.457
CLV_NRD_NRD_1	502	504	PF00675	0.327
CLV_NRD_NRD_1	832	834	PF00675	0.436
CLV_NRD_NRD_1	992	994	PF00675	0.474
CLV_PCSK_FUR_1	378	382	PF00082	0.467
CLV_PCSK_KEX2_1	257	259	PF00082	0.289
CLV_PCSK_KEX2_1	338	340	PF00082	0.351
CLV_PCSK_KEX2_1	380	382	PF00082	0.460
CLV_PCSK_KEX2_1	504	506	PF00082	0.320
CLV_PCSK_KEX2_1	832	834	PF00082	0.437
CLV_PCSK_KEX2_1	992	994	PF00082	0.566
CLV_PCSK_PC1ET2_1	257	259	PF00082	0.289
CLV_PCSK_PC1ET2_1	380	382	PF00082	0.460
CLV_PCSK_PC1ET2_1	504	506	PF00082	0.274
CLV_PCSK_SKI1_1	1008	1012	PF00082	0.351
CLV_PCSK_SKI1_1	1055	1059	PF00082	0.347
CLV_PCSK_SKI1_1	1118	1122	PF00082	0.349
CLV_PCSK_SKI1_1	130	134	PF00082	0.514
CLV_PCSK_SKI1_1	27	31	PF00082	0.617
CLV_PCSK_SKI1_1	298	302	PF00082	0.282
CLV_PCSK_SKI1_1	338	342	PF00082	0.356
CLV_PCSK_SKI1_1	363	367	PF00082	0.598
CLV_PCSK_SKI1_1	380	384	PF00082	0.623
CLV_PCSK_SKI1_1	538	542	PF00082	0.404
CLV_PCSK_SKI1_1	565	569	PF00082	0.427
CLV_PCSK_SKI1_1	727	731	PF00082	0.391
CLV_PCSK_SKI1_1	783	787	PF00082	0.386
CLV_PCSK_SKI1_1	833	837	PF00082	0.371
CLV_PCSK_SKI1_1	872	876	PF00082	0.370
CLV_PCSK_SKI1_1	997	1001	PF00082	0.372
DEG_SPOP_SBC_1	288	292	PF00917	0.432
DOC_CKS1_1	281	286	PF01111	0.225
DOC_CKS1_1	800	805	PF01111	0.330
DOC_CYCLIN_RxL_1	1052	1062	PF00134	0.397
DOC_CYCLIN_RxL_1	295	305	PF00134	0.337
DOC_CYCLIN_yClb1_LxF_4	727	733	PF00134	0.337
DOC_CYCLIN_yCln2_LP_2	488	494	PF00134	0.292
DOC_MAPK_DCC_7	512	522	PF00069	0.380
DOC_MAPK_gen_1	1002	1011	PF00069	0.495
DOC_MAPK_gen_1	255	263	PF00069	0.435
DOC_MAPK_gen_1	832	838	PF00069	0.304
DOC_MAPK_MEF2A_6	483	492	PF00069	0.397
DOC_MAPK_MEF2A_6	628	637	PF00069	0.449
DOC_PIKK_1	529	536	PF02985	0.304
DOC_PP1_RVXF_1	906	913	PF00149	0.340
DOC_PP2B_LxvP_1	488	491	PF13499	0.292
DOC_PP4_FxxP_1	912	915	PF00568	0.330
DOC_USP7_MATH_1	1123	1127	PF00917	0.500
DOC_USP7_MATH_1	309	313	PF00917	0.397
DOC_USP7_MATH_1	398	402	PF00917	0.477
DOC_USP7_MATH_1	510	514	PF00917	0.397
DOC_USP7_MATH_1	550	554	PF00917	0.448
DOC_USP7_UBL2_3	1121	1125	PF12436	0.370
DOC_USP7_UBL2_3	628	632	PF12436	0.392
DOC_USP7_UBL2_3	64	68	PF12436	0.321
DOC_USP7_UBL2_3	723	727	PF12436	0.482
DOC_WW_Pin1_4	280	285	PF00397	0.362
DOC_WW_Pin1_4	33	38	PF00397	0.662
DOC_WW_Pin1_4	513	518	PF00397	0.330
DOC_WW_Pin1_4	596	601	PF00397	0.519
DOC_WW_Pin1_4	799	804	PF00397	0.330
DOC_WW_Pin1_4	812	817	PF00397	0.330
DOC_WW_Pin1_4	826	831	PF00397	0.340
DOC_WW_Pin1_4	942	947	PF00397	0.340
LIG_14-3-3_CanoR_1	207	212	PF00244	0.273
LIG_14-3-3_CanoR_1	538	543	PF00244	0.409
LIG_14-3-3_CanoR_1	690	698	PF00244	0.445
LIG_Actin_WH2_2	1083	1098	PF00022	0.397
LIG_Actin_WH2_2	41	59	PF00022	0.422
LIG_BIR_II_1	1	5	PF00653	0.748
LIG_BRCT_BRCA1_1	1038	1042	PF00533	0.449
LIG_BRCT_BRCA1_1	1066	1070	PF00533	0.375
LIG_BRCT_BRCA1_1	289	293	PF00533	0.397
LIG_Clathr_ClatBox_1	637	641	PF01394	0.330
LIG_FHA_1	1082	1088	PF00498	0.346
LIG_FHA_1	1090	1096	PF00498	0.342
LIG_FHA_1	324	330	PF00498	0.304
LIG_FHA_1	428	434	PF00498	0.330
LIG_FHA_1	471	477	PF00498	0.400
LIG_FHA_1	53	59	PF00498	0.389
LIG_FHA_1	552	558	PF00498	0.318
LIG_FHA_1	615	621	PF00498	0.487
LIG_FHA_1	646	652	PF00498	0.330
LIG_FHA_1	658	664	PF00498	0.332
LIG_FHA_1	800	806	PF00498	0.348
LIG_FHA_1	843	849	PF00498	0.337
LIG_FHA_1	889	895	PF00498	0.360
LIG_FHA_2	1054	1060	PF00498	0.335
LIG_FHA_2	1134	1140	PF00498	0.458
LIG_FHA_2	271	277	PF00498	0.330
LIG_FHA_2	348	354	PF00498	0.469
LIG_FHA_2	547	553	PF00498	0.367
LIG_FHA_2	946	952	PF00498	0.292
LIG_Integrin_RGD_1	932	934	PF01839	0.451
LIG_Integrin_RGDW_4	932	935	PF00362	0.451
LIG_LIR_Apic_2	660	665	PF02991	0.355
LIG_LIR_Gen_1	210	218	PF02991	0.289
LIG_LIR_Gen_1	259	266	PF02991	0.366
LIG_LIR_Gen_1	274	285	PF02991	0.309
LIG_LIR_Gen_1	79	88	PF02991	0.372
LIG_LIR_Gen_1	887	897	PF02991	0.383
LIG_LIR_Nem_3	1051	1057	PF02991	0.352
LIG_LIR_Nem_3	210	216	PF02991	0.529
LIG_LIR_Nem_3	259	263	PF02991	0.366
LIG_LIR_Nem_3	274	280	PF02991	0.309
LIG_LIR_Nem_3	342	347	PF02991	0.467
LIG_LIR_Nem_3	352	357	PF02991	0.389
LIG_LIR_Nem_3	79	84	PF02991	0.353
LIG_LIR_Nem_3	887	892	PF02991	0.422
LIG_OCRL_FandH_1	993	1005	PF00620	0.512
LIG_PCNA_yPIPBox_3	853	864	PF02747	0.326
LIG_Pex14_2	1070	1074	PF04695	0.357
LIG_Pex14_2	725	729	PF04695	0.352
LIG_PTB_Apo_2	340	347	PF02174	0.552
LIG_PTB_Phospho_1	340	346	PF10480	0.552
LIG_REV1ctd_RIR_1	450	459	PF16727	0.423
LIG_REV1ctd_RIR_1	997	1006	PF16727	0.352
LIG_SH2_CRK	1054	1058	PF00017	0.429
LIG_SH2_CRK	714	718	PF00017	0.365
LIG_SH2_CRK	944	948	PF00017	0.365
LIG_SH2_GRB2like	277	280	PF00017	0.336
LIG_SH2_GRB2like	733	736	PF00017	0.350
LIG_SH2_NCK_1	213	217	PF00017	0.449
LIG_SH2_NCK_1	944	948	PF00017	0.365
LIG_SH2_PTP2	797	800	PF00017	0.390
LIG_SH2_SRC	213	216	PF00017	0.412
LIG_SH2_SRC	320	323	PF00017	0.330
LIG_SH2_STAP1	1061	1065	PF00017	0.409
LIG_SH2_STAP1	213	217	PF00017	0.373
LIG_SH2_STAP1	277	281	PF00017	0.340
LIG_SH2_STAP1	289	293	PF00017	0.340
LIG_SH2_STAP1	316	320	PF00017	0.333
LIG_SH2_STAP1	54	58	PF00017	0.365
LIG_SH2_STAT3	607	610	PF00017	0.449
LIG_SH2_STAT5	203	206	PF00017	0.362
LIG_SH2_STAT5	346	349	PF00017	0.464
LIG_SH2_STAT5	354	357	PF00017	0.397
LIG_SH2_STAT5	54	57	PF00017	0.399
LIG_SH2_STAT5	662	665	PF00017	0.389
LIG_SH2_STAT5	714	717	PF00017	0.367
LIG_SH2_STAT5	797	800	PF00017	0.335
LIG_SH2_STAT5	807	810	PF00017	0.321
LIG_SH3_3	1026	1032	PF00018	0.304
LIG_SH3_3	1096	1102	PF00018	0.327
LIG_SH3_3	238	244	PF00018	0.352
LIG_SH3_3	57	63	PF00018	0.456
LIG_SH3_3	650	656	PF00018	0.330
LIG_SH3_3	828	834	PF00018	0.449
LIG_SUMO_SIM_par_1	366	374	PF11976	0.440
LIG_SUMO_SIM_par_1	412	417	PF11976	0.331
LIG_SUMO_SIM_par_1	472	480	PF11976	0.352
LIG_SUMO_SIM_par_1	490	496	PF11976	0.274
LIG_SUMO_SIM_par_1	890	896	PF11976	0.451
LIG_SUMO_SIM_par_1	9	14	PF11976	0.615
LIG_SUMO_SIM_par_1	945	952	PF11976	0.304
LIG_TRAF2_2	1080	1085	PF00917	0.440
LIG_TYR_ITIM	1052	1057	PF00017	0.365
LIG_TYR_ITIM	211	216	PF00017	0.443
LIG_UBA3_1	189	197	PF00899	0.392
LIG_WRC_WIRS_1	88	93	PF05994	0.420
MOD_CDK_SPxK_1	826	832	PF00069	0.449
MOD_CDK_SPxxK_3	280	287	PF00069	0.225
MOD_CDK_SPxxK_3	33	40	PF00069	0.545
MOD_CDK_SPxxK_3	826	833	PF00069	0.449
MOD_CK1_1	1036	1042	PF00069	0.489
MOD_CK1_1	1050	1056	PF00069	0.316
MOD_CK1_1	164	170	PF00069	0.389
MOD_CK1_1	475	481	PF00069	0.359
MOD_CK1_1	513	519	PF00069	0.397
MOD_CK1_1	576	582	PF00069	0.484
MOD_CK1_1	613	619	PF00069	0.415
MOD_CK1_1	657	663	PF00069	0.488
MOD_CK1_1	691	697	PF00069	0.330
MOD_CK1_1	707	713	PF00069	0.330
MOD_CK1_1	826	832	PF00069	0.485
MOD_CK1_1	945	951	PF00069	0.306
MOD_CK1_1	95	101	PF00069	0.342
MOD_CK2_1	1053	1059	PF00069	0.354
MOD_CK2_1	1133	1139	PF00069	0.462
MOD_CK2_1	546	552	PF00069	0.393
MOD_CK2_1	855	861	PF00069	0.330
MOD_CK2_1	87	93	PF00069	0.221
MOD_CK2_1	882	888	PF00069	0.541
MOD_GlcNHglycan	1096	1099	PF01048	0.426
MOD_GlcNHglycan	111	114	PF01048	0.348
MOD_GlcNHglycan	1143	1146	PF01048	0.710
MOD_GlcNHglycan	16	19	PF01048	0.551
MOD_GlcNHglycan	226	229	PF01048	0.352
MOD_GlcNHglycan	273	276	PF01048	0.330
MOD_GlcNHglycan	400	403	PF01048	0.329
MOD_GlcNHglycan	425	428	PF01048	0.345
MOD_GlcNHglycan	430	433	PF01048	0.342
MOD_GlcNHglycan	459	462	PF01048	0.335
MOD_GlcNHglycan	575	578	PF01048	0.462
MOD_GlcNHglycan	6	9	PF01048	0.582
MOD_GlcNHglycan	670	673	PF01048	0.354
MOD_GlcNHglycan	706	709	PF01048	0.374
MOD_GlcNHglycan	78	81	PF01048	0.337
MOD_GlcNHglycan	816	819	PF01048	0.339
MOD_GlcNHglycan	824	828	PF01048	0.341
MOD_GlcNHglycan	971	974	PF01048	0.337
MOD_GSK3_1	1053	1060	PF00069	0.375
MOD_GSK3_1	1133	1140	PF00069	0.470
MOD_GSK3_1	164	171	PF00069	0.365
MOD_GSK3_1	283	290	PF00069	0.414
MOD_GSK3_1	349	356	PF00069	0.456
MOD_GSK3_1	423	430	PF00069	0.332
MOD_GSK3_1	472	479	PF00069	0.450
MOD_GSK3_1	48	55	PF00069	0.474
MOD_GSK3_1	520	527	PF00069	0.292
MOD_GSK3_1	534	541	PF00069	0.304
MOD_GSK3_1	546	553	PF00069	0.318
MOD_GSK3_1	588	595	PF00069	0.336
MOD_GSK3_1	609	616	PF00069	0.355
MOD_GSK3_1	801	808	PF00069	0.295
MOD_GSK3_1	819	826	PF00069	0.330
MOD_GSK3_1	83	90	PF00069	0.416
MOD_GSK3_1	884	891	PF00069	0.477
MOD_N-GLC_1	126	131	PF02516	0.412
MOD_N-GLC_1	168	173	PF02516	0.395
MOD_N-GLC_1	229	234	PF02516	0.444
MOD_N-GLC_1	534	539	PF02516	0.311
MOD_N-GLC_1	657	662	PF02516	0.381
MOD_N-GLC_1	717	722	PF02516	0.440
MOD_NEK2_1	1089	1094	PF00069	0.377
MOD_NEK2_1	168	173	PF00069	0.456
MOD_NEK2_1	470	475	PF00069	0.388
MOD_NEK2_1	48	53	PF00069	0.428
MOD_NEK2_1	551	556	PF00069	0.399
MOD_NEK2_1	668	673	PF00069	0.330
MOD_NEK2_1	717	722	PF00069	0.475
MOD_NEK2_1	87	92	PF00069	0.371
MOD_NEK2_1	918	923	PF00069	0.309
MOD_NEK2_2	309	314	PF00069	0.349
MOD_PIKK_1	493	499	PF00454	0.274
MOD_PIKK_1	691	697	PF00454	0.429
MOD_PIKK_1	789	795	PF00454	0.436
MOD_PK_1	229	235	PF00069	0.225
MOD_PK_1	472	478	PF00069	0.352
MOD_PK_1	742	748	PF00069	0.289
MOD_PKA_1	879	885	PF00069	0.538
MOD_PKA_2	224	230	PF00069	0.340
MOD_PKA_2	294	300	PF00069	0.290
MOD_PKA_2	347	353	PF00069	0.454
MOD_PKA_2	454	460	PF00069	0.330
MOD_PKA_2	668	674	PF00069	0.330
MOD_Plk_1	1050	1056	PF00069	0.352
MOD_Plk_1	168	174	PF00069	0.373
MOD_Plk_1	201	207	PF00069	0.235
MOD_Plk_1	229	235	PF00069	0.245
MOD_Plk_1	352	358	PF00069	0.457
MOD_Plk_1	887	893	PF00069	0.496
MOD_Plk_1	95	101	PF00069	0.338
MOD_Plk_2-3	369	375	PF00069	0.608
MOD_Plk_4	490	496	PF00069	0.344
MOD_Plk_4	538	544	PF00069	0.387
MOD_Plk_4	576	582	PF00069	0.428
MOD_Plk_4	599	605	PF00069	0.338
MOD_Plk_4	6	12	PF00069	0.651
MOD_Plk_4	83	89	PF00069	0.368
MOD_Plk_4	855	861	PF00069	0.352
MOD_Plk_4	872	878	PF00069	0.386
MOD_Plk_4	888	894	PF00069	0.371
MOD_ProDKin_1	280	286	PF00069	0.362
MOD_ProDKin_1	33	39	PF00069	0.656
MOD_ProDKin_1	513	519	PF00069	0.330
MOD_ProDKin_1	596	602	PF00069	0.519
MOD_ProDKin_1	799	805	PF00069	0.330
MOD_ProDKin_1	812	818	PF00069	0.330
MOD_ProDKin_1	826	832	PF00069	0.340
MOD_ProDKin_1	942	948	PF00069	0.340
MOD_SUMO_rev_2	417	422	PF00179	0.366
MOD_SUMO_rev_2	978	984	PF00179	0.360
TRG_DiLeu_BaEn_2	1037	1043	PF01217	0.449
TRG_DiLeu_BaLyEn_6	568	573	PF01217	0.289
TRG_DiLeu_BaLyEn_6	936	941	PF01217	0.289
TRG_ENDOCYTIC_2	1054	1057	PF00928	0.355
TRG_ENDOCYTIC_2	213	216	PF00928	0.449
TRG_ENDOCYTIC_2	277	280	PF00928	0.338
TRG_ENDOCYTIC_2	316	319	PF00928	0.346
TRG_ENDOCYTIC_2	714	717	PF00928	0.365
TRG_ENDOCYTIC_2	767	770	PF00928	0.387
TRG_ENDOCYTIC_2	797	800	PF00928	0.348
TRG_ENDOCYTIC_2	944	947	PF00928	0.365
TRG_ER_diArg_1	337	339	PF00400	0.372
TRG_ER_diArg_1	831	833	PF00400	0.434
TRG_ER_diArg_1	992	994	PF00400	0.517
TRG_NLS_MonoExtN_4	378	384	PF00514	0.551
TRG_Pf-PMV_PEXEL_1	1055	1059	PF00026	0.415
TRG_Pf-PMV_PEXEL_1	142	146	PF00026	0.362
TRG_Pf-PMV_PEXEL_1	298	302	PF00026	0.391
TRG_Pf-PMV_PEXEL_1	483	487	PF00026	0.449
TRG_PTS1	1144	1147	PF00515	0.700

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P690	Leptomonas seymouri	80%	100%
A0A0N1I186	Leptomonas seymouri	59%	93%
A0A0S4IK89	Bodo saltans	55%	96%
A0A0S4IZL1	Bodo saltans	56%	96%
A0A1X0NJ99	Trypanosomatidae	59%	93%
A0A3Q8IJ78	Leishmania donovani	98%	100%
A0A3S5H7Y6	Leishmania donovani	59%	96%
A0A422NIA0	Trypanosoma rangeli	62%	89%
A4HMB4	Leishmania braziliensis	59%	96%
A4HMD9	Leishmania braziliensis	90%	100%
A4IAY0	Leishmania infantum	59%	96%
A4IB12	Leishmania infantum	98%	100%
C9ZNL3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
D0A275	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	93%
E9AEQ8	Leishmania major	60%	96%
E9B5W3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	59%	96%
E9B5Z7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
V5DJK9	Trypanosoma cruzi	62%	94%