Dolichyl-diphosphooligosaccharide--protein glycotransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

oligosaccharyl transferase subunit, putative

Species:

Leishmania major

UniProt:

E9AET9_LEIMA

TriTrypDb:

LmjF.35.1160 , LMJLV39_350017400 , LMJSD75_350017100

Length:

857

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 36

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 26
NetGPI	no	yes: 0, no: 26

Cellular components
Term	Name	Level	Count
GO:0005635	nuclear envelope	4	5
GO:0005783	endoplasmic reticulum	5	5
GO:0016020	membrane	2	27
GO:0031967	organelle envelope	3	5
GO:0031975	envelope	2	5
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5
GO:0110165	cellular anatomical entity	1	27

Expansion

Sequence features

E9AET9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AET9

Function

Biological processes
Term	Name	Level	Count
GO:0006486	protein glycosylation	4	27
GO:0006807	nitrogen compound metabolic process	2	27
GO:0008152	metabolic process	1	27
GO:0019538	protein metabolic process	3	27
GO:0036211	protein modification process	4	27
GO:0043170	macromolecule metabolic process	3	27
GO:0043412	macromolecule modification	4	27
GO:0043413	macromolecule glycosylation	3	27
GO:0044238	primary metabolic process	2	27
GO:0070085	glycosylation	2	27
GO:0071704	organic substance metabolic process	2	27
GO:1901564	organonitrogen compound metabolic process	3	27

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	27
GO:0004576	oligosaccharyl transferase activity	5	27
GO:0004579	dolichyl-diphosphooligosaccharide-protein glycotransferase activity	6	27
GO:0005488	binding	1	27
GO:0016740	transferase activity	2	27
GO:0016757	glycosyltransferase activity	3	27
GO:0016758	hexosyltransferase activity	4	27
GO:0043167	ion binding	2	27
GO:0043169	cation binding	3	27
GO:0046872	metal ion binding	4	27

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	652	656	PF00656	0.267
CLV_C14_Caspase3-7	8	12	PF00656	0.575
CLV_NRD_NRD_1	3	5	PF00675	0.378
CLV_NRD_NRD_1	753	755	PF00675	0.598
CLV_NRD_NRD_1	834	836	PF00675	0.645
CLV_NRD_NRD_1	848	850	PF00675	0.517
CLV_PCSK_KEX2_1	3	5	PF00082	0.513
CLV_PCSK_KEX2_1	753	755	PF00082	0.598
CLV_PCSK_KEX2_1	848	850	PF00082	0.561
CLV_PCSK_PC1ET2_1	3	5	PF00082	0.513
CLV_PCSK_PC1ET2_1	753	755	PF00082	0.479
CLV_PCSK_SKI1_1	396	400	PF00082	0.447
CLV_PCSK_SKI1_1	41	45	PF00082	0.415
CLV_PCSK_SKI1_1	47	51	PF00082	0.359
CLV_PCSK_SKI1_1	473	477	PF00082	0.408
CLV_PCSK_SKI1_1	534	538	PF00082	0.305
CLV_PCSK_SKI1_1	556	560	PF00082	0.295
CLV_PCSK_SKI1_1	669	673	PF00082	0.499
CLV_PCSK_SKI1_1	755	759	PF00082	0.626
CLV_PCSK_SKI1_1	776	780	PF00082	0.604
CLV_Separin_Metazoa	345	349	PF03568	0.284
DEG_ODPH_VHL_1	382	395	PF01847	0.367
DEG_SCF_TRCP1_1	11	17	PF00400	0.592
DEG_SPOP_SBC_1	139	143	PF00917	0.474
DOC_AGCK_PIF_2	124	129	PF00069	0.293
DOC_CYCLIN_RxL_1	393	403	PF00134	0.254
DOC_CYCLIN_RxL_1	470	478	PF00134	0.254
DOC_MAPK_FxFP_2	59	62	PF00069	0.491
DOC_MAPK_gen_1	244	253	PF00069	0.359
DOC_MAPK_gen_1	348	355	PF00069	0.484
DOC_MAPK_gen_1	356	365	PF00069	0.498
DOC_MAPK_gen_1	753	759	PF00069	0.379
DOC_MAPK_HePTP_8	355	367	PF00069	0.457
DOC_MAPK_JIP1_4	608	614	PF00069	0.160
DOC_MAPK_MEF2A_6	358	367	PF00069	0.508
DOC_MAPK_MEF2A_6	556	563	PF00069	0.494
DOC_PP1_RVXF_1	452	458	PF00149	0.496
DOC_PP1_RVXF_1	631	638	PF00149	0.279
DOC_PP4_FxxP_1	59	62	PF00568	0.497
DOC_USP7_MATH_1	138	142	PF00917	0.338
DOC_USP7_MATH_1	16	20	PF00917	0.690
DOC_USP7_MATH_1	193	197	PF00917	0.266
DOC_USP7_MATH_1	322	326	PF00917	0.279
DOC_USP7_MATH_1	547	551	PF00917	0.403
DOC_USP7_MATH_1	580	584	PF00917	0.328
DOC_USP7_MATH_1	587	591	PF00917	0.332
DOC_USP7_MATH_1	693	697	PF00917	0.364
DOC_USP7_UBL2_3	511	515	PF12436	0.379
DOC_WW_Pin1_4	318	323	PF00397	0.279
DOC_WW_Pin1_4	34	39	PF00397	0.606
DOC_WW_Pin1_4	417	422	PF00397	0.232
DOC_WW_Pin1_4	448	453	PF00397	0.401
DOC_WW_Pin1_4	625	630	PF00397	0.268
DOC_WW_Pin1_4	670	675	PF00397	0.307
DOC_WW_Pin1_4	699	704	PF00397	0.346
DOC_WW_Pin1_4	734	739	PF00397	0.329
LIG_14-3-3_CanoR_1	244	250	PF00244	0.303
LIG_14-3-3_CanoR_1	290	294	PF00244	0.531
LIG_14-3-3_CanoR_1	299	304	PF00244	0.405
LIG_14-3-3_CanoR_1	366	371	PF00244	0.453
LIG_14-3-3_CanoR_1	4	10	PF00244	0.569
LIG_14-3-3_CanoR_1	650	654	PF00244	0.294
LIG_14-3-3_CanoR_1	707	713	PF00244	0.313
LIG_14-3-3_CanoR_1	741	745	PF00244	0.372
LIG_14-3-3_CanoR_1	89	94	PF00244	0.279
LIG_Actin_WH2_2	611	627	PF00022	0.219
LIG_AP2alpha_1	542	546	PF02296	0.364
LIG_AP2alpha_2	104	106	PF02296	0.279
LIG_BRCT_BRCA1_1	120	124	PF00533	0.380
LIG_BRCT_BRCA1_1	452	456	PF00533	0.430
LIG_BRCT_BRCA1_1	575	579	PF00533	0.288
LIG_BRCT_BRCA1_1	582	586	PF00533	0.230
LIG_deltaCOP1_diTrp_1	422	427	PF00928	0.224
LIG_FHA_1	181	187	PF00498	0.366
LIG_FHA_1	432	438	PF00498	0.427
LIG_FHA_1	567	573	PF00498	0.416
LIG_FHA_1	670	676	PF00498	0.313
LIG_FHA_2	46	52	PF00498	0.554
LIG_FHA_2	490	496	PF00498	0.278
LIG_FHA_2	626	632	PF00498	0.257
LIG_FHA_2	829	835	PF00498	0.362
LIG_IRF3_LxIS_1	374	380	PF10401	0.224
LIG_LIR_Apic_2	385	390	PF02991	0.305
LIG_LIR_Apic_2	732	736	PF02991	0.382
LIG_LIR_Apic_2	804	808	PF02991	0.461
LIG_LIR_Gen_1	117	127	PF02991	0.340
LIG_LIR_Gen_1	206	215	PF02991	0.432
LIG_LIR_Gen_1	235	243	PF02991	0.332
LIG_LIR_Gen_1	422	433	PF02991	0.228
LIG_LIR_Gen_1	687	693	PF02991	0.351
LIG_LIR_Gen_1	711	721	PF02991	0.394
LIG_LIR_Gen_1	762	772	PF02991	0.416
LIG_LIR_Gen_1	81	91	PF02991	0.418
LIG_LIR_Nem_3	104	109	PF02991	0.280
LIG_LIR_Nem_3	117	123	PF02991	0.277
LIG_LIR_Nem_3	125	129	PF02991	0.281
LIG_LIR_Nem_3	131	135	PF02991	0.262
LIG_LIR_Nem_3	141	147	PF02991	0.250
LIG_LIR_Nem_3	185	191	PF02991	0.342
LIG_LIR_Nem_3	206	210	PF02991	0.380
LIG_LIR_Nem_3	235	240	PF02991	0.317
LIG_LIR_Nem_3	302	307	PF02991	0.300
LIG_LIR_Nem_3	321	326	PF02991	0.273
LIG_LIR_Nem_3	385	391	PF02991	0.279
LIG_LIR_Nem_3	422	428	PF02991	0.216
LIG_LIR_Nem_3	445	450	PF02991	0.427
LIG_LIR_Nem_3	453	459	PF02991	0.395
LIG_LIR_Nem_3	52	58	PF02991	0.617
LIG_LIR_Nem_3	687	691	PF02991	0.284
LIG_LIR_Nem_3	711	716	PF02991	0.318
LIG_LIR_Nem_3	762	767	PF02991	0.360
LIG_LIR_Nem_3	769	775	PF02991	0.305
LIG_LIR_Nem_3	81	86	PF02991	0.370
LIG_LYPXL_yS_3	144	147	PF13949	0.375
LIG_MLH1_MIPbox_1	120	124	PF16413	0.380
LIG_NRBOX	328	334	PF00104	0.377
LIG_NRP_CendR_1	856	857	PF00754	0.572
LIG_PCNA_yPIPBox_3	604	618	PF02747	0.249
LIG_Pex14_1	120	124	PF04695	0.279
LIG_Pex14_1	638	642	PF04695	0.268
LIG_Pex14_1	64	68	PF04695	0.475
LIG_Pex14_2	103	107	PF04695	0.258
LIG_Pex14_2	123	127	PF04695	0.215
LIG_Pex14_2	542	546	PF04695	0.401
LIG_Pex14_2	55	59	PF04695	0.624
LIG_Pex14_2	575	579	PF04695	0.232
LIG_PTB_Apo_2	707	714	PF02174	0.317
LIG_PTB_Phospho_1	707	713	PF10480	0.313
LIG_RPA_C_Insects	743	758	PF08784	0.282
LIG_SH2_CRK	733	737	PF00017	0.476
LIG_SH2_CRK	772	776	PF00017	0.354
LIG_SH2_CRK	96	100	PF00017	0.352
LIG_SH2_NCK_1	733	737	PF00017	0.416
LIG_SH2_PTP2	269	272	PF00017	0.352
LIG_SH2_PTP2	688	691	PF00017	0.338
LIG_SH2_SRC	98	101	PF00017	0.379
LIG_SH2_STAP1	301	305	PF00017	0.352
LIG_SH2_STAP1	768	772	PF00017	0.453
LIG_SH2_STAT3	640	643	PF00017	0.316
LIG_SH2_STAT5	237	240	PF00017	0.375
LIG_SH2_STAT5	269	272	PF00017	0.352
LIG_SH2_STAT5	301	304	PF00017	0.452
LIG_SH2_STAT5	305	308	PF00017	0.436
LIG_SH2_STAT5	387	390	PF00017	0.313
LIG_SH2_STAT5	466	469	PF00017	0.323
LIG_SH2_STAT5	617	620	PF00017	0.334
LIG_SH2_STAT5	688	691	PF00017	0.338
LIG_SH2_STAT5	98	101	PF00017	0.425
LIG_SH3_3	169	175	PF00018	0.375
LIG_SH3_3	310	316	PF00018	0.414
LIG_SH3_3	378	384	PF00018	0.343
LIG_SH3_3	415	421	PF00018	0.224
LIG_SH3_3	59	65	PF00018	0.331
LIG_SH3_3	715	721	PF00018	0.503
LIG_SH3_3	788	794	PF00018	0.450
LIG_SH3_3	795	801	PF00018	0.454
LIG_SUMO_SIM_par_1	439	446	PF11976	0.224
LIG_SUMO_SIM_par_1	473	478	PF11976	0.236
LIG_SxIP_EBH_1	203	216	PF03271	0.349
LIG_TRAF2_1	841	844	PF00917	0.364
LIG_TYR_ITSM	233	240	PF00017	0.316
LIG_UBA3_1	744	753	PF00899	0.498
LIG_WRC_WIRS_1	233	238	PF05994	0.316
LIG_WRC_WIRS_1	444	449	PF05994	0.199
MOD_CDC14_SPxK_1	321	324	PF00782	0.474
MOD_CDC14_SPxK_1	451	454	PF00782	0.293
MOD_CDK_SPxK_1	318	324	PF00069	0.332
MOD_CDK_SPxK_1	448	454	PF00069	0.299
MOD_CDK_SPxxK_3	34	41	PF00069	0.460
MOD_CDK_SPxxK_3	734	741	PF00069	0.468
MOD_CK1_1	14	20	PF00069	0.689
MOD_CK1_1	194	200	PF00069	0.358
MOD_CK1_1	242	248	PF00069	0.352
MOD_CK1_1	505	511	PF00069	0.350
MOD_CK1_1	573	579	PF00069	0.374
MOD_CK1_1	581	587	PF00069	0.356
MOD_CK1_1	734	740	PF00069	0.408
MOD_CK1_1	784	790	PF00069	0.411
MOD_CK2_1	409	415	PF00069	0.286
MOD_CK2_1	489	495	PF00069	0.406
MOD_CK2_1	624	630	PF00069	0.254
MOD_CK2_1	838	844	PF00069	0.490
MOD_Cter_Amidation	1	4	PF01082	0.696
MOD_Cter_Amidation	751	754	PF01082	0.302
MOD_GlcNHglycan	11	14	PF01048	0.551
MOD_GlcNHglycan	120	123	PF01048	0.459
MOD_GlcNHglycan	192	196	PF01048	0.357
MOD_GlcNHglycan	21	25	PF01048	0.573
MOD_GlcNHglycan	460	463	PF01048	0.302
MOD_GlcNHglycan	477	480	PF01048	0.167
MOD_GlcNHglycan	527	530	PF01048	0.450
MOD_GlcNHglycan	695	698	PF01048	0.480
MOD_GlcNHglycan	783	786	PF01048	0.459
MOD_GSK3_1	114	121	PF00069	0.421
MOD_GSK3_1	16	23	PF00069	0.622
MOD_GSK3_1	187	194	PF00069	0.342
MOD_GSK3_1	28	35	PF00069	0.647
MOD_GSK3_1	289	296	PF00069	0.391
MOD_GSK3_1	318	325	PF00069	0.357
MOD_GSK3_1	498	505	PF00069	0.438
MOD_GSK3_1	5	12	PF00069	0.637
MOD_GSK3_1	566	573	PF00069	0.354
MOD_GSK3_1	574	581	PF00069	0.288
MOD_N-GLC_1	534	539	PF02516	0.171
MOD_N-GLC_1	648	653	PF02516	0.314
MOD_N-GLC_1	781	786	PF02516	0.405
MOD_N-GLC_2	169	171	PF02516	0.436
MOD_N-GLC_2	224	226	PF02516	0.332
MOD_NEK2_1	147	152	PF00069	0.332
MOD_NEK2_1	205	210	PF00069	0.362
MOD_NEK2_1	214	219	PF00069	0.355
MOD_NEK2_1	232	237	PF00069	0.214
MOD_NEK2_1	377	382	PF00069	0.290
MOD_NEK2_1	409	414	PF00069	0.290
MOD_NEK2_1	442	447	PF00069	0.297
MOD_NEK2_1	458	463	PF00069	0.324
MOD_NEK2_1	475	480	PF00069	0.206
MOD_NEK2_1	489	494	PF00069	0.389
MOD_NEK2_1	546	551	PF00069	0.199
MOD_NEK2_1	557	562	PF00069	0.192
MOD_NEK2_1	566	571	PF00069	0.381
MOD_NEK2_1	624	629	PF00069	0.278
MOD_NEK2_1	68	73	PF00069	0.409
MOD_NEK2_1	740	745	PF00069	0.391
MOD_NEK2_1	78	83	PF00069	0.305
MOD_NEK2_1	9	14	PF00069	0.482
MOD_NEK2_2	239	244	PF00069	0.316
MOD_PIKK_1	28	34	PF00454	0.734
MOD_PK_1	366	372	PF00069	0.417
MOD_PK_1	89	95	PF00069	0.381
MOD_PKA_2	245	251	PF00069	0.382
MOD_PKA_2	289	295	PF00069	0.405
MOD_PKA_2	603	609	PF00069	0.354
MOD_PKA_2	624	630	PF00069	0.278
MOD_PKA_2	649	655	PF00069	0.316
MOD_PKA_2	740	746	PF00069	0.456
MOD_PKA_2	850	856	PF00069	0.376
MOD_PKB_1	364	372	PF00069	0.379
MOD_Plk_1	648	654	PF00069	0.329
MOD_Plk_4	128	134	PF00069	0.332
MOD_Plk_4	232	238	PF00069	0.316
MOD_Plk_4	281	287	PF00069	0.423
MOD_Plk_4	293	299	PF00069	0.254
MOD_Plk_4	436	442	PF00069	0.342
MOD_Plk_4	443	449	PF00069	0.318
MOD_Plk_4	489	495	PF00069	0.340
MOD_Plk_4	498	504	PF00069	0.364
MOD_Plk_4	570	576	PF00069	0.269
MOD_Plk_4	677	683	PF00069	0.449
MOD_Plk_4	68	74	PF00069	0.269
MOD_Plk_4	708	714	PF00069	0.367
MOD_Plk_4	740	746	PF00069	0.445
MOD_Plk_4	759	765	PF00069	0.325
MOD_Plk_4	801	807	PF00069	0.459
MOD_ProDKin_1	318	324	PF00069	0.332
MOD_ProDKin_1	34	40	PF00069	0.508
MOD_ProDKin_1	417	423	PF00069	0.266
MOD_ProDKin_1	448	454	PF00069	0.222
MOD_ProDKin_1	625	631	PF00069	0.316
MOD_ProDKin_1	670	676	PF00069	0.380
MOD_ProDKin_1	699	705	PF00069	0.420
MOD_ProDKin_1	734	740	PF00069	0.394
MOD_SUMO_rev_2	528	536	PF00179	0.171
TRG_DiLeu_BaEn_1	677	682	PF01217	0.391
TRG_DiLeu_BaLyEn_6	470	475	PF01217	0.283
TRG_ENDOCYTIC_2	144	147	PF00928	0.419
TRG_ENDOCYTIC_2	237	240	PF00928	0.316
TRG_ENDOCYTIC_2	259	262	PF00928	0.316
TRG_ENDOCYTIC_2	269	272	PF00928	0.316
TRG_ENDOCYTIC_2	301	304	PF00928	0.316
TRG_ENDOCYTIC_2	621	624	PF00928	0.316
TRG_ENDOCYTIC_2	688	691	PF00928	0.338
TRG_ENDOCYTIC_2	713	716	PF00928	0.404
TRG_ENDOCYTIC_2	772	775	PF00928	0.347
TRG_ENDOCYTIC_2	96	99	PF00928	0.352
TRG_ER_diArg_1	243	246	PF00400	0.468
TRG_ER_diArg_1	347	350	PF00400	0.357
TRG_ER_diArg_1	355	358	PF00400	0.316
TRG_ER_diArg_1	363	366	PF00400	0.237
TRG_ER_diArg_1	393	396	PF00400	0.301
TRG_ER_diArg_1	847	849	PF00400	0.463
TRG_NES_CRM1_1	327	342	PF08389	0.474
TRG_Pf-PMV_PEXEL_1	396	401	PF00026	0.317

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IZ72	Bodo saltans	51%	100%
A0A1X0NFU7	Trypanosomatidae	56%	100%
A0A3Q8IHT0	Leishmania donovani	73%	99%
A0A3Q8II34	Leishmania donovani	55%	100%
A0A3Q8ILY7	Leishmania donovani	73%	100%
A0A3Q8IV37	Leishmania donovani	91%	100%
A0A422MX14	Trypanosoma rangeli	55%	100%
A4HFF9	Leishmania braziliensis	65%	100%
A4HMD5	Leishmania braziliensis	71%	100%
A4HMD6	Leishmania braziliensis	55%	100%
A4HMD7	Leishmania braziliensis	80%	100%
A4IB08	Leishmania infantum	73%	99%
A4IB09	Leishmania infantum	73%	100%
A4IB10	Leishmania infantum	56%	100%
C9ZNL1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
C9ZQ40	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E2RG47	Canis lupus familiaris	29%	100%
E9AET6	Leishmania major	69%	99%
E9AET7	Leishmania major	76%	100%
E9AET8	Leishmania major	57%	100%
E9AHU4	Leishmania infantum	91%	100%
E9B5Z2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	68%	99%
E9B5Z3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	74%	100%
E9B5Z4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	55%	100%
E9B5Z5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
F1PJP5	Canis lupus familiaris	29%	100%
O94335	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
P39007	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
P46975	Caenorhabditis elegans	30%	100%
P46977	Homo sapiens	30%	100%
P46978	Mus musculus	30%	100%
Q2KJI2	Bos taurus	30%	100%
Q3TDQ1	Mus musculus	30%	100%
Q54NM9	Dictyostelium discoideum	31%	100%
Q5RCE2	Pongo abelii	30%	100%
Q6F2Z1	Oryza sativa subsp. japonica	29%	100%
Q7XQ88	Oryza sativa subsp. japonica	32%	100%
Q8TCJ2	Homo sapiens	29%	100%
Q93ZY3	Arabidopsis thaliana	32%	100%
Q9FX21	Arabidopsis thaliana	31%	100%
V5BDM6	Trypanosoma cruzi	56%	100%