Dolichyl-diphosphooligosaccharide--protein glycotransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

oligosaccharyl transferase-like protein

Species:

Leishmania major

UniProt:

E9AET8_LEIMA

TriTrypDb:

LmjF.35.1150 , LMJLV39_350017600 , LMJSD75_350017000

Length:

790

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 36

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 26
NetGPI	no	yes: 0, no: 26

Cellular components
Term	Name	Level	Count
GO:0005635	nuclear envelope	4	5
GO:0005783	endoplasmic reticulum	5	5
GO:0016020	membrane	2	27
GO:0031967	organelle envelope	3	5
GO:0031975	envelope	2	5
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5
GO:0110165	cellular anatomical entity	1	27

Expansion

Sequence features

E9AET8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AET8

Function

Biological processes
Term	Name	Level	Count
GO:0006486	protein glycosylation	4	27
GO:0006807	nitrogen compound metabolic process	2	27
GO:0008152	metabolic process	1	27
GO:0019538	protein metabolic process	3	27
GO:0036211	protein modification process	4	27
GO:0043170	macromolecule metabolic process	3	27
GO:0043412	macromolecule modification	4	27
GO:0043413	macromolecule glycosylation	3	27
GO:0044238	primary metabolic process	2	27
GO:0070085	glycosylation	2	27
GO:0071704	organic substance metabolic process	2	27
GO:1901564	organonitrogen compound metabolic process	3	27

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	27
GO:0004576	oligosaccharyl transferase activity	5	27
GO:0004579	dolichyl-diphosphooligosaccharide-protein glycotransferase activity	6	27
GO:0005488	binding	1	27
GO:0016740	transferase activity	2	27
GO:0016757	glycosyltransferase activity	3	27
GO:0016758	hexosyltransferase activity	4	27
GO:0043167	ion binding	2	27
GO:0043169	cation binding	3	27
GO:0046872	metal ion binding	4	27

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	572	576	PF00656	0.268
CLV_NRD_NRD_1	289	291	PF00675	0.218
CLV_NRD_NRD_1	470	472	PF00675	0.366
CLV_NRD_NRD_1	511	513	PF00675	0.370
CLV_PCSK_KEX2_1	288	290	PF00082	0.238
CLV_PCSK_KEX2_1	470	472	PF00082	0.305
CLV_PCSK_KEX2_1	652	654	PF00082	0.556
CLV_PCSK_KEX2_1	660	662	PF00082	0.588
CLV_PCSK_PC1ET2_1	470	472	PF00082	0.299
CLV_PCSK_PC1ET2_1	652	654	PF00082	0.556
CLV_PCSK_PC1ET2_1	660	662	PF00082	0.588
CLV_PCSK_SKI1_1	430	434	PF00082	0.387
CLV_PCSK_SKI1_1	589	593	PF00082	0.499
CLV_PCSK_SKI1_1	723	727	PF00082	0.618
CLV_PCSK_SKI1_1	776	780	PF00082	0.585
DEG_APCC_DBOX_1	429	437	PF00400	0.226
DEG_ODPH_VHL_1	321	332	PF01847	0.368
DEG_SPOP_SBC_1	79	83	PF00917	0.475
DOC_AGCK_PIF_2	64	69	PF00069	0.295
DOC_MAPK_FxFP_2	367	370	PF00069	0.290
DOC_MAPK_gen_1	184	193	PF00069	0.360
DOC_MAPK_gen_1	295	305	PF00069	0.499
DOC_MAPK_gen_1	660	667	PF00069	0.405
DOC_MAPK_MEF2A_6	298	307	PF00069	0.510
DOC_MAPK_MEF2A_6	487	496	PF00069	0.339
DOC_MAPK_MEF2A_6	512	521	PF00069	0.229
DOC_MAPK_RevD_3	517	533	PF00069	0.195
DOC_PP1_RVXF_1	551	558	PF00149	0.281
DOC_PP4_FxxP_1	326	329	PF00568	0.304
DOC_PP4_FxxP_1	367	370	PF00568	0.451
DOC_PP4_FxxP_1	715	718	PF00568	0.228
DOC_USP7_MATH_1	138	142	PF00917	0.347
DOC_USP7_MATH_1	262	266	PF00917	0.281
DOC_USP7_MATH_1	376	380	PF00917	0.417
DOC_USP7_MATH_1	407	411	PF00917	0.430
DOC_USP7_MATH_1	462	466	PF00917	0.380
DOC_USP7_MATH_1	643	647	PF00917	0.313
DOC_USP7_MATH_1	659	663	PF00917	0.417
DOC_USP7_MATH_1	78	82	PF00917	0.416
DOC_WW_Pin1_4	258	263	PF00397	0.281
DOC_WW_Pin1_4	545	550	PF00397	0.269
DOC_WW_Pin1_4	590	595	PF00397	0.307
DOC_WW_Pin1_4	621	626	PF00397	0.338
DOC_WW_Pin1_4	714	719	PF00397	0.379
LIG_14-3-3_CanoR_1	184	190	PF00244	0.304
LIG_14-3-3_CanoR_1	230	234	PF00244	0.533
LIG_14-3-3_CanoR_1	239	244	PF00244	0.407
LIG_14-3-3_CanoR_1	343	349	PF00244	0.316
LIG_14-3-3_CanoR_1	387	392	PF00244	0.433
LIG_14-3-3_CanoR_1	570	574	PF00244	0.296
LIG_14-3-3_CanoR_1	629	635	PF00244	0.319
LIG_14-3-3_CanoR_1	663	668	PF00244	0.408
LIG_14-3-3_CanoR_1	782	786	PF00244	0.438
LIG_AP2alpha_2	44	46	PF02296	0.281
LIG_BRCT_BRCA1_1	10	14	PF00533	0.303
LIG_BRCT_BRCA1_1	143	147	PF00533	0.416
LIG_BRCT_BRCA1_1	60	64	PF00533	0.382
LIG_Clathr_ClatBox_1	304	308	PF01394	0.178
LIG_eIF4E_1	435	441	PF01652	0.408
LIG_eIF4E_1	681	687	PF01652	0.288
LIG_FAT_LD_1	433	441	PF03623	0.395
LIG_FHA_1	3	9	PF00498	0.600
LIG_FHA_1	423	429	PF00498	0.308
LIG_FHA_1	476	482	PF00498	0.421
LIG_FHA_1	590	596	PF00498	0.313
LIG_FHA_1	696	702	PF00498	0.352
LIG_FHA_2	345	351	PF00498	0.292
LIG_FHA_2	459	465	PF00498	0.404
LIG_FHA_2	546	552	PF00498	0.258
LIG_FHA_2	703	709	PF00498	0.335
LIG_GBD_Chelix_1	687	695	PF00786	0.406
LIG_LIR_Apic_2	751	755	PF02991	0.465
LIG_LIR_Gen_1	144	155	PF02991	0.433
LIG_LIR_Gen_1	175	183	PF02991	0.334
LIG_LIR_Gen_1	394	402	PF02991	0.235
LIG_LIR_Gen_1	439	449	PF02991	0.480
LIG_LIR_Gen_1	473	484	PF02991	0.416
LIG_LIR_Gen_1	57	67	PF02991	0.342
LIG_LIR_Gen_1	607	613	PF02991	0.355
LIG_LIR_Gen_1	615	626	PF02991	0.415
LIG_LIR_Gen_1	633	643	PF02991	0.400
LIG_LIR_Gen_1	708	718	PF02991	0.422
LIG_LIR_Nem_3	144	150	PF02991	0.357
LIG_LIR_Nem_3	175	180	PF02991	0.318
LIG_LIR_Nem_3	242	247	PF02991	0.302
LIG_LIR_Nem_3	261	266	PF02991	0.275
LIG_LIR_Nem_3	43	49	PF02991	0.283
LIG_LIR_Nem_3	535	541	PF02991	0.257
LIG_LIR_Nem_3	57	63	PF02991	0.279
LIG_LIR_Nem_3	607	611	PF02991	0.287
LIG_LIR_Nem_3	615	621	PF02991	0.368
LIG_LIR_Nem_3	633	638	PF02991	0.320
LIG_LIR_Nem_3	65	69	PF02991	0.282
LIG_LIR_Nem_3	708	714	PF02991	0.370
LIG_LIR_Nem_3	71	75	PF02991	0.264
LIG_LIR_Nem_3	773	778	PF02991	0.373
LIG_LIR_Nem_3	81	87	PF02991	0.252
LIG_LYPXL_yS_3	84	87	PF13949	0.376
LIG_MLH1_MIPbox_1	60	64	PF16413	0.382
LIG_NRBOX	268	274	PF00104	0.379
LIG_NRBOX	432	438	PF00104	0.226
LIG_NRBOX	492	498	PF00104	0.325
LIG_NRP_CendR_1	789	790	PF00754	0.603
LIG_Pex14_1	431	435	PF04695	0.226
LIG_Pex14_1	558	562	PF04695	0.269
LIG_Pex14_1	60	64	PF04695	0.281
LIG_Pex14_2	367	371	PF04695	0.395
LIG_Pex14_2	63	67	PF04695	0.217
LIG_Pex14_2	711	715	PF04695	0.257
LIG_PTB_Apo_2	385	392	PF02174	0.404
LIG_PTB_Apo_2	629	636	PF02174	0.323
LIG_PTB_Phospho_1	385	391	PF10480	0.404
LIG_PTB_Phospho_1	629	635	PF10480	0.319
LIG_REV1ctd_RIR_1	324	328	PF16727	0.217
LIG_RPA_C_Fungi	658	670	PF08784	0.466
LIG_SH2_CRK	36	40	PF00017	0.353
LIG_SH2_CRK	399	403	PF00017	0.305
LIG_SH2_PTP2	209	212	PF00017	0.353
LIG_SH2_PTP2	608	611	PF00017	0.340
LIG_SH2_SRC	681	684	PF00017	0.361
LIG_SH2_STAP1	10	14	PF00017	0.255
LIG_SH2_STAP1	241	245	PF00017	0.353
LIG_SH2_STAP1	26	30	PF00017	0.326
LIG_SH2_STAP1	391	395	PF00017	0.366
LIG_SH2_STAT3	560	563	PF00017	0.318
LIG_SH2_STAT5	177	180	PF00017	0.376
LIG_SH2_STAT5	209	212	PF00017	0.353
LIG_SH2_STAT5	241	244	PF00017	0.453
LIG_SH2_STAT5	245	248	PF00017	0.437
LIG_SH2_STAT5	38	41	PF00017	0.426
LIG_SH2_STAT5	389	392	PF00017	0.313
LIG_SH2_STAT5	405	408	PF00017	0.324
LIG_SH2_STAT5	435	438	PF00017	0.332
LIG_SH2_STAT5	541	544	PF00017	0.331
LIG_SH2_STAT5	608	611	PF00017	0.340
LIG_SH2_STAT5	617	620	PF00017	0.444
LIG_SH3_3	109	115	PF00018	0.376
LIG_SH3_3	250	256	PF00018	0.416
LIG_SH3_3	637	643	PF00018	0.512
LIG_SH3_3	677	683	PF00018	0.410
LIG_SH3_3	742	748	PF00018	0.454
LIG_SUMO_SIM_anti_2	299	305	PF11976	0.342
LIG_SUMO_SIM_anti_2	347	353	PF11976	0.206
LIG_SUMO_SIM_par_1	314	320	PF11976	0.214
LIG_TRAF2_1	461	464	PF00917	0.458
LIG_TRAF2_1	669	672	PF00917	0.468
LIG_TYR_ITIM	397	402	PF00017	0.250
LIG_TYR_ITSM	173	180	PF00017	0.318
LIG_UBA3_1	721	726	PF00899	0.389
LIG_WRC_WIRS_1	173	178	PF05994	0.318
MOD_CDC14_SPxK_1	261	264	PF00782	0.475
MOD_CDK_SPxK_1	258	264	PF00069	0.334
MOD_CK1_1	119	125	PF00069	0.254
MOD_CK1_1	141	147	PF00069	0.359
MOD_CK1_1	182	188	PF00069	0.353
MOD_CK1_1	448	454	PF00069	0.366
MOD_CK1_1	731	737	PF00069	0.416
MOD_CK2_1	279	285	PF00069	0.470
MOD_CK2_1	458	464	PF00069	0.387
MOD_CK2_1	544	550	PF00069	0.256
MOD_CK2_1	702	708	PF00069	0.337
MOD_GlcNHglycan	122	125	PF01048	0.426
MOD_GlcNHglycan	132	136	PF01048	0.358
MOD_GlcNHglycan	378	381	PF01048	0.220
MOD_GlcNHglycan	500	503	PF01048	0.336
MOD_GlcNHglycan	60	63	PF01048	0.460
MOD_GlcNHglycan	611	614	PF01048	0.391
MOD_GlcNHglycan	740	743	PF01048	0.513
MOD_GlcNHglycan	785	788	PF01048	0.463
MOD_GSK3_1	116	123	PF00069	0.374
MOD_GSK3_1	141	148	PF00069	0.373
MOD_GSK3_1	229	236	PF00069	0.392
MOD_GSK3_1	258	265	PF00069	0.359
MOD_GSK3_1	387	394	PF00069	0.239
MOD_GSK3_1	4	11	PF00069	0.473
MOD_GSK3_1	458	465	PF00069	0.401
MOD_GSK3_1	54	61	PF00069	0.423
MOD_GSK3_1	609	616	PF00069	0.258
MOD_GSK3_1	659	666	PF00069	0.629
MOD_GSK3_1	695	702	PF00069	0.396
MOD_GSK3_1	781	788	PF00069	0.466
MOD_N-GLC_1	387	392	PF02516	0.238
MOD_N-GLC_1	568	573	PF02516	0.316
MOD_N-GLC_1	621	626	PF02516	0.306
MOD_N-GLC_1	706	711	PF02516	0.304
MOD_N-GLC_1	728	733	PF02516	0.414
MOD_N-GLC_2	109	111	PF02516	0.437
MOD_N-GLC_2	164	166	PF02516	0.334
MOD_NEK2_1	120	125	PF00069	0.392
MOD_NEK2_1	154	159	PF00069	0.357
MOD_NEK2_1	172	177	PF00069	0.215
MOD_NEK2_1	279	284	PF00069	0.214
MOD_NEK2_1	436	441	PF00069	0.318
MOD_NEK2_1	498	503	PF00069	0.308
MOD_NEK2_1	544	549	PF00069	0.279
MOD_NEK2_1	695	700	PF00069	0.406
MOD_NEK2_1	706	711	PF00069	0.250
MOD_NEK2_1	8	13	PF00069	0.410
MOD_NEK2_1	87	92	PF00069	0.334
MOD_NEK2_2	179	184	PF00069	0.318
MOD_NEK2_2	407	412	PF00069	0.294
MOD_PIKK_1	405	411	PF00454	0.244
MOD_PIKK_1	643	649	PF00454	0.412
MOD_PK_1	29	35	PF00069	0.446
MOD_PK_1	482	488	PF00069	0.196
MOD_PK_1	663	669	PF00069	0.500
MOD_PKA_1	482	488	PF00069	0.237
MOD_PKA_2	185	191	PF00069	0.383
MOD_PKA_2	229	235	PF00069	0.407
MOD_PKA_2	294	300	PF00069	0.370
MOD_PKA_2	498	504	PF00069	0.349
MOD_PKA_2	544	550	PF00069	0.279
MOD_PKA_2	569	575	PF00069	0.318
MOD_PKA_2	781	787	PF00069	0.477
MOD_PKB_1	661	669	PF00069	0.510
MOD_Plk_1	387	393	PF00069	0.226
MOD_Plk_1	568	574	PF00069	0.331
MOD_Plk_1	706	712	PF00069	0.317
MOD_Plk_4	141	147	PF00069	0.323
MOD_Plk_4	172	178	PF00069	0.318
MOD_Plk_4	221	227	PF00069	0.425
MOD_Plk_4	233	239	PF00069	0.255
MOD_Plk_4	344	350	PF00069	0.452
MOD_Plk_4	391	397	PF00069	0.272
MOD_Plk_4	436	442	PF00069	0.361
MOD_Plk_4	597	603	PF00069	0.455
MOD_Plk_4	613	619	PF00069	0.475
MOD_Plk_4	630	636	PF00069	0.371
MOD_Plk_4	68	74	PF00069	0.334
MOD_Plk_4	682	688	PF00069	0.469
MOD_Plk_4	706	712	PF00069	0.317
MOD_Plk_4	748	754	PF00069	0.461
MOD_ProDKin_1	258	264	PF00069	0.334
MOD_ProDKin_1	545	551	PF00069	0.318
MOD_ProDKin_1	590	596	PF00069	0.378
MOD_ProDKin_1	621	627	PF00069	0.409
MOD_ProDKin_1	714	720	PF00069	0.472
MOD_SUMO_rev_2	530	534	PF00179	0.214
TRG_DiLeu_BaEn_1	299	304	PF01217	0.244
TRG_DiLeu_BaEn_1	597	602	PF01217	0.396
TRG_DiLeu_BaEn_1	682	687	PF01217	0.315
TRG_ENDOCYTIC_2	177	180	PF00928	0.318
TRG_ENDOCYTIC_2	199	202	PF00928	0.318
TRG_ENDOCYTIC_2	209	212	PF00928	0.318
TRG_ENDOCYTIC_2	241	244	PF00928	0.318
TRG_ENDOCYTIC_2	36	39	PF00928	0.353
TRG_ENDOCYTIC_2	399	402	PF00928	0.263
TRG_ENDOCYTIC_2	476	479	PF00928	0.244
TRG_ENDOCYTIC_2	541	544	PF00928	0.318
TRG_ENDOCYTIC_2	608	611	PF00928	0.340
TRG_ENDOCYTIC_2	635	638	PF00928	0.404
TRG_ENDOCYTIC_2	84	87	PF00928	0.421
TRG_ER_diArg_1	183	186	PF00400	0.469
TRG_ER_diArg_1	28	31	PF00400	0.429
TRG_ER_diArg_1	288	290	PF00400	0.357
TRG_NES_CRM1_1	267	281	PF08389	0.475
TRG_NES_CRM1_1	719	733	PF08389	0.294
TRG_NLS_MonoCore_2	651	656	PF00514	0.428

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IZ72	Bodo saltans	43%	96%
A0A1X0NFU7	Trypanosomatidae	48%	93%
A0A3Q8IHT0	Leishmania donovani	55%	92%
A0A3Q8II34	Leishmania donovani	94%	99%
A0A3Q8ILY7	Leishmania donovani	57%	100%
A0A3Q8IV37	Leishmania donovani	57%	94%
A0A422MX14	Trypanosoma rangeli	48%	95%
A4HFF9	Leishmania braziliensis	54%	92%
A4HMD5	Leishmania braziliensis	54%	100%
A4HMD6	Leishmania braziliensis	79%	96%
A4HMD7	Leishmania braziliensis	55%	93%
A4IB08	Leishmania infantum	55%	92%
A4IB09	Leishmania infantum	57%	100%
A4IB10	Leishmania infantum	94%	99%
C9ZNL1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	96%
C9ZQ40	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E2RG47	Canis lupus familiaris	30%	96%
E9AET6	Leishmania major	57%	100%
E9AET7	Leishmania major	59%	99%
E9AET9	Leishmania major	57%	92%
E9AHU4	Leishmania infantum	57%	94%
E9B5Z2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	55%	92%
E9B5Z3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	57%	94%
E9B5Z4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	99%
E9B5Z5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	56%	93%
F1PJP5	Canis lupus familiaris	30%	100%
O94335	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
P39007	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
P46975	Caenorhabditis elegans	29%	100%
P46977	Homo sapiens	30%	100%
P46978	Mus musculus	30%	100%
Q2KJI2	Bos taurus	30%	100%
Q3TDQ1	Mus musculus	30%	96%
Q54NM9	Dictyostelium discoideum	30%	100%
Q5RCE2	Pongo abelii	30%	100%
Q6F2Z1	Oryza sativa subsp. japonica	28%	100%
Q7XQ88	Oryza sativa subsp. japonica	30%	100%
Q8TCJ2	Homo sapiens	31%	96%
Q93ZY3	Arabidopsis thaliana	32%	100%
Q9FX21	Arabidopsis thaliana	29%	100%
V5BDM6	Trypanosoma cruzi	49%	95%