Dolichyl-diphosphooligosaccharide--protein glycotransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

oligosaccharyl transferase-like protein

Species:

Leishmania major

UniProt:

E9AET7_LEIMA

TriTrypDb:

LmjF.35.1140 , LMJLV39_350017400 , LMJSD75_350016800

Length:

773

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 36

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 26
NetGPI	no	yes: 0, no: 26

Cellular components
Term	Name	Level	Count
GO:0005635	nuclear envelope	4	5
GO:0005783	endoplasmic reticulum	5	5
GO:0016020	membrane	2	27
GO:0031967	organelle envelope	3	5
GO:0031975	envelope	2	5
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5
GO:0110165	cellular anatomical entity	1	27

Expansion

Sequence features

E9AET7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AET7

Function

Biological processes
Term	Name	Level	Count
GO:0006486	protein glycosylation	4	27
GO:0006807	nitrogen compound metabolic process	2	27
GO:0008152	metabolic process	1	27
GO:0019538	protein metabolic process	3	27
GO:0036211	protein modification process	4	27
GO:0043170	macromolecule metabolic process	3	27
GO:0043412	macromolecule modification	4	27
GO:0043413	macromolecule glycosylation	3	27
GO:0044238	primary metabolic process	2	27
GO:0070085	glycosylation	2	27
GO:0071704	organic substance metabolic process	2	27
GO:1901564	organonitrogen compound metabolic process	3	27

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	27
GO:0004576	oligosaccharyl transferase activity	5	27
GO:0004579	dolichyl-diphosphooligosaccharide-protein glycotransferase activity	6	27
GO:0005488	binding	1	27
GO:0016740	transferase activity	2	27
GO:0016757	glycosyltransferase activity	3	27
GO:0016758	hexosyltransferase activity	4	27
GO:0043167	ion binding	2	27
GO:0043169	cation binding	3	27
GO:0046872	metal ion binding	4	27

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	582	586	PF00656	0.312
CLV_MEL_PAP_1	491	497	PF00089	0.250
CLV_NRD_NRD_1	380	382	PF00675	0.296
CLV_PCSK_KEX2_1	454	456	PF00082	0.511
CLV_PCSK_PC1ET2_1	454	456	PF00082	0.502
CLV_PCSK_SKI1_1	326	330	PF00082	0.447
CLV_PCSK_SKI1_1	480	484	PF00082	0.445
CLV_PCSK_SKI1_1	529	533	PF00082	0.565
CLV_PCSK_SKI1_1	599	603	PF00082	0.497
CLV_PCSK_SKI1_1	685	689	PF00082	0.623
CLV_PCSK_SKI1_1	706	710	PF00082	0.602
CLV_Separin_Metazoa	275	279	PF03568	0.284
DEG_Nend_Nbox_1	1	3	PF02207	0.279
DEG_ODPH_VHL_1	312	325	PF01847	0.367
DEG_SCF_FBW7_1	506	512	PF00400	0.213
DEG_SPOP_SBC_1	69	73	PF00917	0.474
DOC_AGCK_PIF_1	357	362	PF00069	0.224
DOC_AGCK_PIF_2	54	59	PF00069	0.293
DOC_CKS1_1	506	511	PF01111	0.224
DOC_CYCLIN_RxL_1	323	333	PF00134	0.254
DOC_CYCLIN_yCln2_LP_2	408	414	PF00134	0.254
DOC_MAPK_DCC_7	483	493	PF00069	0.530
DOC_MAPK_FxFP_2	360	363	PF00069	0.289
DOC_MAPK_gen_1	174	183	PF00069	0.359
DOC_MAPK_gen_1	278	285	PF00069	0.484
DOC_MAPK_gen_1	286	295	PF00069	0.498
DOC_MAPK_gen_1	381	390	PF00069	0.402
DOC_MAPK_gen_1	480	488	PF00069	0.639
DOC_MAPK_HePTP_8	285	297	PF00069	0.457
DOC_MAPK_MEF2A_6	288	297	PF00069	0.508
DOC_MAPK_MEF2A_6	494	501	PF00069	0.494
DOC_PP1_RVXF_1	527	533	PF00149	0.385
DOC_PP1_RVXF_1	561	568	PF00149	0.303
DOC_PP4_FxxP_1	360	363	PF00568	0.440
DOC_PP4_FxxP_1	525	528	PF00568	0.375
DOC_USP7_MATH_1	252	256	PF00917	0.279
DOC_USP7_MATH_1	442	446	PF00917	0.662
DOC_USP7_MATH_1	478	482	PF00917	0.564
DOC_USP7_MATH_1	509	513	PF00917	0.386
DOC_USP7_MATH_1	623	627	PF00917	0.361
DOC_USP7_MATH_1	68	72	PF00917	0.338
DOC_WW_Pin1_4	248	253	PF00397	0.279
DOC_WW_Pin1_4	347	352	PF00397	0.232
DOC_WW_Pin1_4	434	439	PF00397	0.495
DOC_WW_Pin1_4	505	510	PF00397	0.473
DOC_WW_Pin1_4	555	560	PF00397	0.328
DOC_WW_Pin1_4	600	605	PF00397	0.305
DOC_WW_Pin1_4	629	634	PF00397	0.342
DOC_WW_Pin1_4	664	669	PF00397	0.328
LIG_14-3-3_CanoR_1	174	180	PF00244	0.303
LIG_14-3-3_CanoR_1	19	24	PF00244	0.279
LIG_14-3-3_CanoR_1	220	224	PF00244	0.531
LIG_14-3-3_CanoR_1	229	234	PF00244	0.405
LIG_14-3-3_CanoR_1	296	301	PF00244	0.453
LIG_14-3-3_CanoR_1	347	351	PF00244	0.258
LIG_14-3-3_CanoR_1	517	525	PF00244	0.347
LIG_14-3-3_CanoR_1	580	584	PF00244	0.331
LIG_14-3-3_CanoR_1	637	643	PF00244	0.315
LIG_14-3-3_CanoR_1	671	675	PF00244	0.372
LIG_Actin_WH2_2	479	496	PF00022	0.493
LIG_Actin_WH2_2	5	21	PF00022	0.212
LIG_Actin_WH2_2	541	557	PF00022	0.265
LIG_AP2alpha_2	34	36	PF02296	0.279
LIG_BRCT_BRCA1_1	50	54	PF00533	0.380
LIG_DCNL_PONY_1	1	4	PF03556	0.264
LIG_deltaCOP1_diTrp_1	426	435	PF00928	0.584
LIG_FHA_1	111	117	PF00498	0.366
LIG_FHA_1	359	365	PF00498	0.435
LIG_FHA_1	415	421	PF00498	0.307
LIG_FHA_1	506	512	PF00498	0.491
LIG_FHA_1	600	606	PF00498	0.311
LIG_FHA_1	682	688	PF00498	0.397
LIG_FHA_2	556	562	PF00498	0.311
LIG_IRF3_LxIS_1	304	310	PF10401	0.224
LIG_LIR_Apic_2	315	320	PF02991	0.305
LIG_LIR_Apic_2	359	363	PF02991	0.201
LIG_LIR_Apic_2	433	438	PF02991	0.544
LIG_LIR_Apic_2	524	528	PF02991	0.380
LIG_LIR_Apic_2	662	666	PF02991	0.381
LIG_LIR_Apic_2	734	738	PF02991	0.458
LIG_LIR_Gen_1	165	173	PF02991	0.332
LIG_LIR_Gen_1	426	436	PF02991	0.551
LIG_LIR_Gen_1	47	57	PF02991	0.340
LIG_LIR_Gen_1	549	559	PF02991	0.302
LIG_LIR_Gen_1	617	623	PF02991	0.350
LIG_LIR_Gen_1	641	651	PF02991	0.400
LIG_LIR_Gen_1	692	702	PF02991	0.414
LIG_LIR_Nem_3	165	170	PF02991	0.317
LIG_LIR_Nem_3	232	237	PF02991	0.300
LIG_LIR_Nem_3	251	256	PF02991	0.273
LIG_LIR_Nem_3	315	321	PF02991	0.279
LIG_LIR_Nem_3	34	39	PF02991	0.280
LIG_LIR_Nem_3	426	432	PF02991	0.495
LIG_LIR_Nem_3	47	53	PF02991	0.277
LIG_LIR_Nem_3	549	554	PF02991	0.307
LIG_LIR_Nem_3	55	59	PF02991	0.281
LIG_LIR_Nem_3	61	65	PF02991	0.262
LIG_LIR_Nem_3	617	621	PF02991	0.283
LIG_LIR_Nem_3	641	646	PF02991	0.319
LIG_LIR_Nem_3	692	697	PF02991	0.359
LIG_LIR_Nem_3	699	705	PF02991	0.303
LIG_LIR_Nem_3	71	77	PF02991	0.250
LIG_LYPXL_yS_3	74	77	PF13949	0.375
LIG_MLH1_MIPbox_1	50	54	PF16413	0.380
LIG_NRBOX	258	264	PF00104	0.377
LIG_PCNA_PIPBox_1	541	550	PF02747	0.288
LIG_PCNA_yPIPBox_3	541	555	PF02747	0.276
LIG_Pex14_1	50	54	PF04695	0.279
LIG_Pex14_1	568	572	PF04695	0.293
LIG_Pex14_2	33	37	PF04695	0.258
LIG_Pex14_2	356	360	PF04695	0.385
LIG_Pex14_2	53	57	PF04695	0.215
LIG_PTB_Apo_2	637	644	PF02174	0.320
LIG_PTB_Phospho_1	637	643	PF10480	0.315
LIG_SH2_CRK	26	30	PF00017	0.293
LIG_SH2_CRK	663	667	PF00017	0.382
LIG_SH2_CRK	702	706	PF00017	0.293
LIG_SH2_NCK_1	663	667	PF00017	0.339
LIG_SH2_PTP2	199	202	PF00017	0.351
LIG_SH2_PTP2	618	621	PF00017	0.282
LIG_SH2_SRC	28	31	PF00017	0.313
LIG_SH2_SRC	547	550	PF00017	0.293
LIG_SH2_STAP1	231	235	PF00017	0.351
LIG_SH2_STAP1	354	358	PF00017	0.202
LIG_SH2_STAP1	425	429	PF00017	0.445
LIG_SH2_STAP1	698	702	PF00017	0.362
LIG_SH2_STAT3	510	513	PF00017	0.468
LIG_SH2_STAT3	570	573	PF00017	0.284
LIG_SH2_STAT5	10	13	PF00017	0.319
LIG_SH2_STAT5	167	170	PF00017	0.375
LIG_SH2_STAT5	199	202	PF00017	0.351
LIG_SH2_STAT5	231	234	PF00017	0.452
LIG_SH2_STAT5	235	238	PF00017	0.435
LIG_SH2_STAT5	28	31	PF00017	0.345
LIG_SH2_STAT5	317	320	PF00017	0.266
LIG_SH2_STAT5	397	400	PF00017	0.323
LIG_SH2_STAT5	510	513	PF00017	0.332
LIG_SH2_STAT5	547	550	PF00017	0.316
LIG_SH2_STAT5	618	621	PF00017	0.282
LIG_SH3_3	240	246	PF00018	0.414
LIG_SH3_3	308	314	PF00018	0.343
LIG_SH3_3	503	509	PF00018	0.477
LIG_SH3_3	645	651	PF00018	0.411
LIG_SH3_3	718	724	PF00018	0.361
LIG_SH3_3	725	731	PF00018	0.364
LIG_SH3_3	99	105	PF00018	0.375
LIG_SUMO_SIM_anti_2	115	120	PF11976	0.409
LIG_SUMO_SIM_anti_2	403	409	PF11976	0.209
LIG_SUMO_SIM_par_1	113	120	PF11976	0.259
LIG_SUMO_SIM_par_1	500	505	PF11976	0.389
LIG_SxIP_EBH_1	133	146	PF03271	0.349
LIG_TYR_ITSM	163	170	PF00017	0.316
LIG_UBA3_1	674	683	PF00899	0.398
LIG_WRC_WIRS_1	163	168	PF05994	0.316
LIG_WRC_WIRS_1	5	10	PF05994	0.449
MOD_CDC14_SPxK_1	251	254	PF00782	0.380
MOD_CDK_SPK_2	347	352	PF00069	0.177
MOD_CDK_SPxK_1	248	254	PF00069	0.279
MOD_CDK_SPxxK_3	664	671	PF00069	0.376
MOD_CK1_1	172	178	PF00069	0.351
MOD_CK1_1	392	398	PF00069	0.224
MOD_CK1_1	521	527	PF00069	0.338
MOD_CK1_1	664	670	PF00069	0.333
MOD_CK1_1	714	720	PF00069	0.334
MOD_CK2_1	339	345	PF00069	0.247
MOD_CK2_1	377	383	PF00069	0.402
MOD_CK2_1	554	560	PF00069	0.278
MOD_GlcNHglycan	122	126	PF01048	0.497
MOD_GlcNHglycan	440	443	PF01048	0.370
MOD_GlcNHglycan	444	447	PF01048	0.420
MOD_GlcNHglycan	50	53	PF01048	0.570
MOD_GlcNHglycan	520	523	PF01048	0.565
MOD_GlcNHglycan	625	628	PF01048	0.582
MOD_GlcNHglycan	713	716	PF01048	0.569
MOD_GSK3_1	117	124	PF00069	0.342
MOD_GSK3_1	219	226	PF00069	0.521
MOD_GSK3_1	248	255	PF00069	0.297
MOD_GSK3_1	430	437	PF00069	0.621
MOD_GSK3_1	438	445	PF00069	0.634
MOD_GSK3_1	44	51	PF00069	0.343
MOD_GSK3_1	455	462	PF00069	0.738
MOD_GSK3_1	505	512	PF00069	0.282
MOD_N-GLC_1	578	583	PF02516	0.505
MOD_N-GLC_1	711	716	PF02516	0.531
MOD_N-GLC_2	154	156	PF02516	0.279
MOD_N-GLC_2	99	101	PF02516	0.435
MOD_NEK2_1	123	128	PF00069	0.295
MOD_NEK2_1	135	140	PF00069	0.362
MOD_NEK2_1	144	149	PF00069	0.355
MOD_NEK2_1	162	167	PF00069	0.214
MOD_NEK2_1	307	312	PF00069	0.290
MOD_NEK2_1	339	344	PF00069	0.249
MOD_NEK2_1	358	363	PF00069	0.309
MOD_NEK2_1	366	371	PF00069	0.299
MOD_NEK2_1	377	382	PF00069	0.456
MOD_NEK2_1	4	9	PF00069	0.346
MOD_NEK2_1	502	507	PF00069	0.329
MOD_NEK2_1	554	559	PF00069	0.290
MOD_NEK2_1	670	675	PF00069	0.321
MOD_NEK2_1	77	82	PF00069	0.332
MOD_NEK2_2	169	174	PF00069	0.316
MOD_NEK2_2	478	483	PF00069	0.522
MOD_PIKK_1	455	461	PF00454	0.773
MOD_PIKK_1	509	515	PF00454	0.308
MOD_PK_1	19	25	PF00069	0.314
MOD_PK_1	296	302	PF00069	0.417
MOD_PKA_2	175	181	PF00069	0.315
MOD_PKA_2	219	225	PF00069	0.531
MOD_PKA_2	346	352	PF00069	0.247
MOD_PKA_2	400	406	PF00069	0.477
MOD_PKA_2	554	560	PF00069	0.295
MOD_PKA_2	579	585	PF00069	0.310
MOD_PKA_2	670	676	PF00069	0.367
MOD_PKB_1	294	302	PF00069	0.513
MOD_Plk_1	358	364	PF00069	0.231
MOD_Plk_1	578	584	PF00069	0.317
MOD_Plk_4	162	168	PF00069	0.316
MOD_Plk_4	211	217	PF00069	0.423
MOD_Plk_4	223	229	PF00069	0.423
MOD_Plk_4	366	372	PF00069	0.342
MOD_Plk_4	392	398	PF00069	0.320
MOD_Plk_4	58	64	PF00069	0.279
MOD_Plk_4	607	613	PF00069	0.362
MOD_Plk_4	638	644	PF00069	0.305
MOD_Plk_4	670	676	PF00069	0.360
MOD_Plk_4	689	695	PF00069	0.274
MOD_Plk_4	731	737	PF00069	0.366
MOD_ProDKin_1	248	254	PF00069	0.279
MOD_ProDKin_1	347	353	PF00069	0.232
MOD_ProDKin_1	434	440	PF00069	0.495
MOD_ProDKin_1	505	511	PF00069	0.473
MOD_ProDKin_1	555	561	PF00069	0.329
MOD_ProDKin_1	600	606	PF00069	0.311
MOD_ProDKin_1	629	635	PF00069	0.338
MOD_ProDKin_1	664	670	PF00069	0.323
TRG_DiLeu_BaEn_1	607	612	PF01217	0.320
TRG_DiLeu_BaEn_2	382	388	PF01217	0.402
TRG_DiLeu_BaLyEn_6	401	406	PF01217	0.283
TRG_ENDOCYTIC_2	167	170	PF00928	0.316
TRG_ENDOCYTIC_2	189	192	PF00928	0.316
TRG_ENDOCYTIC_2	199	202	PF00928	0.316
TRG_ENDOCYTIC_2	231	234	PF00928	0.316
TRG_ENDOCYTIC_2	26	29	PF00928	0.293
TRG_ENDOCYTIC_2	354	357	PF00928	0.237
TRG_ENDOCYTIC_2	551	554	PF00928	0.303
TRG_ENDOCYTIC_2	618	621	PF00928	0.282
TRG_ENDOCYTIC_2	643	646	PF00928	0.332
TRG_ENDOCYTIC_2	702	705	PF00928	0.288
TRG_ENDOCYTIC_2	74	77	PF00928	0.419
TRG_ER_diArg_1	173	176	PF00400	0.376
TRG_ER_diArg_1	277	280	PF00400	0.497
TRG_ER_diArg_1	285	288	PF00400	0.468
TRG_ER_diArg_1	293	296	PF00400	0.411
TRG_ER_diArg_1	323	326	PF00400	0.257
TRG_NES_CRM1_1	257	272	PF08389	0.380
TRG_Pf-PMV_PEXEL_1	326	331	PF00026	0.468

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IZ72	Bodo saltans	52%	94%
A0A1X0NFU7	Trypanosomatidae	59%	91%
A0A3Q8IHT0	Leishmania donovani	77%	90%
A0A3Q8II34	Leishmania donovani	58%	97%
A0A3Q8ILY7	Leishmania donovani	92%	99%
A0A3Q8IV37	Leishmania donovani	75%	92%
A0A422MX14	Trypanosoma rangeli	58%	93%
A4HFF9	Leishmania braziliensis	69%	90%
A4HMD5	Leishmania braziliensis	82%	100%
A4HMD6	Leishmania braziliensis	57%	94%
A4HMD7	Leishmania braziliensis	71%	91%
A4IB08	Leishmania infantum	77%	90%
A4IB09	Leishmania infantum	93%	99%
A4IB10	Leishmania infantum	58%	97%
C9ZNL1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	94%
C9ZQ40	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
E2RG47	Canis lupus familiaris	30%	94%
E9AET6	Leishmania major	79%	100%
E9AET8	Leishmania major	59%	98%
E9AET9	Leishmania major	76%	90%
E9AHU4	Leishmania infantum	75%	92%
E9B5Z2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	90%
E9B5Z3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	92%
E9B5Z4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	57%	97%
E9B5Z5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	74%	91%
F1PJP5	Canis lupus familiaris	29%	100%
O94335	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
P39007	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
P46975	Caenorhabditis elegans	31%	100%
P46977	Homo sapiens	29%	100%
P46978	Mus musculus	29%	100%
Q2KJI2	Bos taurus	29%	100%
Q3TDQ1	Mus musculus	31%	94%
Q54NM9	Dictyostelium discoideum	31%	100%
Q5RCE2	Pongo abelii	29%	100%
Q7XQ88	Oryza sativa subsp. japonica	32%	100%
Q8TCJ2	Homo sapiens	31%	94%
Q93ZY3	Arabidopsis thaliana	32%	99%
Q9FX21	Arabidopsis thaliana	30%	100%
V5BDM6	Trypanosoma cruzi	57%	93%