Dolichyl-diphosphooligosaccharide--protein glycotransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

oligosaccharyl transferase-like protein

Species:

Leishmania major

UniProt:

E9AET6_LEIMA

TriTrypDb:

LmjF.35.1130 , LMJLV39_350017400 , LMJSD75_350016700

Length:

852

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 36

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 26
NetGPI	no	yes: 0, no: 26

Cellular components
Term	Name	Level	Count
GO:0005635	nuclear envelope	4	5
GO:0005783	endoplasmic reticulum	5	5
GO:0016020	membrane	2	27
GO:0031967	organelle envelope	3	5
GO:0031975	envelope	2	5
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5
GO:0110165	cellular anatomical entity	1	27

Expansion

Sequence features

E9AET6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AET6

Function

Biological processes
Term	Name	Level	Count
GO:0006486	protein glycosylation	4	27
GO:0006807	nitrogen compound metabolic process	2	27
GO:0008152	metabolic process	1	27
GO:0019538	protein metabolic process	3	27
GO:0036211	protein modification process	4	27
GO:0043170	macromolecule metabolic process	3	27
GO:0043412	macromolecule modification	4	27
GO:0043413	macromolecule glycosylation	3	27
GO:0044238	primary metabolic process	2	27
GO:0070085	glycosylation	2	27
GO:0071704	organic substance metabolic process	2	27
GO:1901564	organonitrogen compound metabolic process	3	27

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	27
GO:0004576	oligosaccharyl transferase activity	5	27
GO:0004579	dolichyl-diphosphooligosaccharide-protein glycotransferase activity	6	27
GO:0005488	binding	1	27
GO:0016740	transferase activity	2	27
GO:0016757	glycosyltransferase activity	3	27
GO:0016758	hexosyltransferase activity	4	27
GO:0043167	ion binding	2	27
GO:0043169	cation binding	3	27
GO:0046872	metal ion binding	4	27

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	661	665	PF00656	0.322
CLV_NRD_NRD_1	560	562	PF00675	0.436
CLV_PCSK_KEX2_1	560	562	PF00082	0.435
CLV_PCSK_SKI1_1	390	394	PF00082	0.457
CLV_PCSK_SKI1_1	608	612	PF00082	0.550
CLV_PCSK_SKI1_1	678	682	PF00082	0.506
CLV_PCSK_SKI1_1	764	768	PF00082	0.602
CLV_PCSK_SKI1_1	785	789	PF00082	0.599
CLV_Separin_Metazoa	339	343	PF03568	0.298
DEG_ODPH_VHL_1	376	389	PF01847	0.367
DEG_SCF_FBW7_2	17	24	PF00400	0.601
DEG_SPOP_SBC_1	133	137	PF00917	0.474
DOC_AGCK_PIF_2	118	123	PF00069	0.303
DOC_CYCLIN_RxL_1	387	397	PF00134	0.265
DOC_MAPK_DCC_7	549	559	PF00069	0.629
DOC_MAPK_gen_1	238	247	PF00069	0.364
DOC_MAPK_gen_1	342	349	PF00069	0.494
DOC_MAPK_gen_1	350	359	PF00069	0.508
DOC_MAPK_gen_1	549	559	PF00069	0.702
DOC_MAPK_HePTP_8	349	361	PF00069	0.458
DOC_MAPK_MEF2A_6	352	361	PF00069	0.509
DOC_MAPK_MEF2A_6	552	559	PF00069	0.737
DOC_MAPK_MEF2A_6	572	581	PF00069	0.461
DOC_MAPK_MEF2A_6	65	72	PF00069	0.207
DOC_PP1_RVXF_1	606	612	PF00149	0.399
DOC_PP1_RVXF_1	640	647	PF00149	0.313
DOC_PP2B_LxvP_1	437	440	PF13499	0.225
DOC_PP4_FxxP_1	604	607	PF00568	0.377
DOC_USP7_MATH_1	132	136	PF00917	0.339
DOC_USP7_MATH_1	22	26	PF00917	0.735
DOC_USP7_MATH_1	316	320	PF00917	0.290
DOC_USP7_MATH_1	36	40	PF00917	0.658
DOC_USP7_MATH_1	539	543	PF00917	0.666
DOC_USP7_MATH_1	702	706	PF00917	0.360
DOC_USP7_MATH_1	75	79	PF00917	0.207
DOC_USP7_UBL2_3	4	8	PF12436	0.627
DOC_WW_Pin1_4	17	22	PF00397	0.630
DOC_WW_Pin1_4	312	317	PF00397	0.290
DOC_WW_Pin1_4	634	639	PF00397	0.338
DOC_WW_Pin1_4	679	684	PF00397	0.308
DOC_WW_Pin1_4	708	713	PF00397	0.341
DOC_WW_Pin1_4	743	748	PF00397	0.341
LIG_14-3-3_CanoR_1	238	244	PF00244	0.312
LIG_14-3-3_CanoR_1	284	288	PF00244	0.539
LIG_14-3-3_CanoR_1	293	298	PF00244	0.416
LIG_14-3-3_CanoR_1	360	365	PF00244	0.460
LIG_14-3-3_CanoR_1	540	550	PF00244	0.676
LIG_14-3-3_CanoR_1	659	663	PF00244	0.342
LIG_14-3-3_CanoR_1	716	722	PF00244	0.322
LIG_14-3-3_CanoR_1	750	754	PF00244	0.382
LIG_14-3-3_CanoR_1	83	88	PF00244	0.290
LIG_Actin_WH2_2	620	636	PF00022	0.267
LIG_AP2alpha_2	98	100	PF02296	0.290
LIG_BRCT_BRCA1_1	114	118	PF00533	0.381
LIG_BRCT_BRCA1_1	60	64	PF00533	0.462
LIG_Clathr_ClatBox_1	182	186	PF01394	0.219
LIG_Clathr_ClatBox_1	554	558	PF01394	0.597
LIG_deltaCOP1_diTrp_1	488	496	PF00928	0.605
LIG_FHA_1	175	181	PF00498	0.379
LIG_FHA_1	460	466	PF00498	0.425
LIG_FHA_1	580	586	PF00498	0.470
LIG_FHA_1	67	73	PF00498	0.409
LIG_FHA_1	679	685	PF00498	0.321
LIG_FHA_1	761	767	PF00498	0.400
LIG_FHA_2	483	489	PF00498	0.278
LIG_FHA_2	542	548	PF00498	0.654
LIG_FHA_2	635	641	PF00498	0.321
LIG_GBD_Chelix_1	579	587	PF00786	0.381
LIG_IRF3_LxIS_1	368	374	PF10401	0.225
LIG_LIR_Apic_2	379	384	PF02991	0.315
LIG_LIR_Apic_2	603	607	PF02991	0.386
LIG_LIR_Apic_2	741	745	PF02991	0.393
LIG_LIR_Apic_2	813	817	PF02991	0.460
LIG_LIR_Gen_1	111	121	PF02991	0.347
LIG_LIR_Gen_1	200	209	PF02991	0.440
LIG_LIR_Gen_1	229	237	PF02991	0.346
LIG_LIR_Gen_1	488	495	PF02991	0.560
LIG_LIR_Gen_1	628	638	PF02991	0.312
LIG_LIR_Gen_1	696	702	PF02991	0.356
LIG_LIR_Gen_1	720	730	PF02991	0.445
LIG_LIR_Gen_1	771	781	PF02991	0.421
LIG_LIR_Nem_3	111	117	PF02991	0.287
LIG_LIR_Nem_3	119	123	PF02991	0.291
LIG_LIR_Nem_3	125	129	PF02991	0.273
LIG_LIR_Nem_3	135	141	PF02991	0.262
LIG_LIR_Nem_3	200	204	PF02991	0.392
LIG_LIR_Nem_3	229	234	PF02991	0.331
LIG_LIR_Nem_3	296	301	PF02991	0.314
LIG_LIR_Nem_3	315	320	PF02991	0.284
LIG_LIR_Nem_3	379	385	PF02991	0.290
LIG_LIR_Nem_3	454	458	PF02991	0.237
LIG_LIR_Nem_3	488	494	PF02991	0.515
LIG_LIR_Nem_3	548	554	PF02991	0.606
LIG_LIR_Nem_3	61	67	PF02991	0.537
LIG_LIR_Nem_3	628	633	PF02991	0.318
LIG_LIR_Nem_3	696	700	PF02991	0.293
LIG_LIR_Nem_3	720	725	PF02991	0.323
LIG_LIR_Nem_3	771	776	PF02991	0.370
LIG_LIR_Nem_3	778	784	PF02991	0.314
LIG_LIR_Nem_3	98	103	PF02991	0.290
LIG_LYPXL_yS_3	138	141	PF13949	0.388
LIG_MLH1_MIPbox_1	114	118	PF16413	0.381
LIG_NRBOX	322	328	PF00104	0.390
LIG_PCNA_PIPBox_1	620	629	PF02747	0.292
LIG_PCNA_yPIPBox_3	620	634	PF02747	0.279
LIG_Pex14_1	114	118	PF04695	0.290
LIG_Pex14_1	647	651	PF04695	0.302
LIG_Pex14_2	117	121	PF04695	0.216
LIG_Pex14_2	491	495	PF04695	0.594
LIG_Pex14_2	97	101	PF04695	0.268
LIG_PTB_Apo_2	716	723	PF02174	0.330
LIG_PTB_Phospho_1	716	722	PF10480	0.325
LIG_SH2_CRK	445	449	PF00017	0.498
LIG_SH2_CRK	551	555	PF00017	0.601
LIG_SH2_CRK	57	61	PF00017	0.485
LIG_SH2_CRK	742	746	PF00017	0.385
LIG_SH2_CRK	781	785	PF00017	0.304
LIG_SH2_CRK	90	94	PF00017	0.303
LIG_SH2_GRB2like	418	421	PF00017	0.288
LIG_SH2_NCK_1	742	746	PF00017	0.342
LIG_SH2_PTP2	263	266	PF00017	0.365
LIG_SH2_PTP2	697	700	PF00017	0.293
LIG_SH2_SRC	421	424	PF00017	0.267
LIG_SH2_SRC	626	629	PF00017	0.296
LIG_SH2_SRC	92	95	PF00017	0.313
LIG_SH2_STAP1	295	299	PF00017	0.365
LIG_SH2_STAP1	445	449	PF00017	0.512
LIG_SH2_STAP1	453	457	PF00017	0.290
LIG_SH2_STAP1	777	781	PF00017	0.404
LIG_SH2_STAT3	649	652	PF00017	0.294
LIG_SH2_STAT5	231	234	PF00017	0.388
LIG_SH2_STAT5	263	266	PF00017	0.365
LIG_SH2_STAT5	295	298	PF00017	0.458
LIG_SH2_STAT5	299	302	PF00017	0.444
LIG_SH2_STAT5	381	384	PF00017	0.276
LIG_SH2_STAT5	418	421	PF00017	0.233
LIG_SH2_STAT5	459	462	PF00017	0.337
LIG_SH2_STAT5	48	51	PF00017	0.641
LIG_SH2_STAT5	626	629	PF00017	0.329
LIG_SH2_STAT5	697	700	PF00017	0.293
LIG_SH2_STAT5	92	95	PF00017	0.352
LIG_SH3_3	163	169	PF00018	0.388
LIG_SH3_3	304	310	PF00018	0.415
LIG_SH3_3	372	378	PF00018	0.344
LIG_SH3_3	582	588	PF00018	0.290
LIG_SH3_3	724	730	PF00018	0.420
LIG_SH3_3	797	803	PF00018	0.372
LIG_SH3_3	804	810	PF00018	0.380
LIG_SUMO_SIM_anti_2	179	184	PF11976	0.419
LIG_SUMO_SIM_anti_2	69	74	PF11976	0.226
LIG_SUMO_SIM_par_1	577	582	PF11976	0.408
LIG_SxIP_EBH_1	197	210	PF03271	0.350
LIG_TRAF2_1	545	548	PF00917	0.605
LIG_TRAF2_2	512	517	PF00917	0.591
LIG_TYR_ITIM	419	424	PF00017	0.203
LIG_TYR_ITSM	227	234	PF00017	0.330
LIG_UBA3_1	753	762	PF00899	0.379
LIG_WRC_WIRS_1	227	232	PF05994	0.330
LIG_WRC_WIRS_1	452	457	PF05994	0.231
MOD_CDC14_SPxK_1	315	318	PF00782	0.381
MOD_CDK_SPxK_1	312	318	PF00069	0.290
MOD_CDK_SPxxK_3	743	750	PF00069	0.388
MOD_CK1_1	236	242	PF00069	0.365
MOD_CK1_1	44	50	PF00069	0.653
MOD_CK1_1	542	548	PF00069	0.644
MOD_CK1_1	58	64	PF00069	0.552
MOD_CK1_1	596	602	PF00069	0.357
MOD_CK1_1	743	749	PF00069	0.346
MOD_CK1_1	793	799	PF00069	0.346
MOD_CK2_1	403	409	PF00069	0.257
MOD_CK2_1	482	488	PF00069	0.416
MOD_CK2_1	541	547	PF00069	0.630
MOD_CK2_1	633	639	PF00069	0.278
MOD_GlcNHglycan	114	117	PF01048	0.570
MOD_GlcNHglycan	537	540	PF01048	0.442
MOD_GlcNHglycan	568	571	PF01048	0.505
MOD_GlcNHglycan	599	602	PF01048	0.590
MOD_GlcNHglycan	704	707	PF01048	0.587
MOD_GlcNHglycan	77	80	PF01048	0.395
MOD_GlcNHglycan	792	795	PF01048	0.580
MOD_GSK3_1	108	115	PF00069	0.350
MOD_GSK3_1	22	29	PF00069	0.725
MOD_GSK3_1	283	290	PF00069	0.530
MOD_GSK3_1	312	319	PF00069	0.307
MOD_GSK3_1	37	44	PF00069	0.671
MOD_GSK3_1	535	542	PF00069	0.770
MOD_GSK3_1	566	573	PF00069	0.649
MOD_GSK3_1	593	600	PF00069	0.341
MOD_GSK3_1	610	617	PF00069	0.368
MOD_N-GLC_1	29	34	PF02516	0.390
MOD_N-GLC_1	657	662	PF02516	0.516
MOD_N-GLC_1	790	795	PF02516	0.542
MOD_N-GLC_2	163	165	PF02516	0.444
MOD_N-GLC_2	218	220	PF02516	0.290
MOD_NEK2_1	141	146	PF00069	0.346
MOD_NEK2_1	199	204	PF00069	0.376
MOD_NEK2_1	208	213	PF00069	0.369
MOD_NEK2_1	226	231	PF00069	0.223
MOD_NEK2_1	371	376	PF00069	0.304
MOD_NEK2_1	403	408	PF00069	0.259
MOD_NEK2_1	451	456	PF00069	0.338
MOD_NEK2_1	593	598	PF00069	0.323
MOD_NEK2_1	633	638	PF00069	0.290
MOD_NEK2_1	749	754	PF00069	0.332
MOD_NEK2_2	233	238	PF00069	0.330
MOD_PIKK_1	610	616	PF00454	0.304
MOD_PK_1	360	366	PF00069	0.427
MOD_PK_1	83	89	PF00069	0.323
MOD_PKA_2	239	245	PF00069	0.324
MOD_PKA_2	283	289	PF00069	0.539
MOD_PKA_2	539	545	PF00069	0.772
MOD_PKA_2	566	572	PF00069	0.646
MOD_PKA_2	633	639	PF00069	0.295
MOD_PKA_2	658	664	PF00069	0.321
MOD_PKA_2	749	755	PF00069	0.378
MOD_PKB_1	358	366	PF00069	0.513
MOD_Plk_1	37	43	PF00069	0.585
MOD_Plk_1	44	50	PF00069	0.544
MOD_Plk_1	657	663	PF00069	0.317
MOD_Plk_4	122	128	PF00069	0.290
MOD_Plk_4	226	232	PF00069	0.330
MOD_Plk_4	275	281	PF00069	0.433
MOD_Plk_4	287	293	PF00069	0.433
MOD_Plk_4	44	50	PF00069	0.700
MOD_Plk_4	451	457	PF00069	0.338
MOD_Plk_4	482	488	PF00069	0.353
MOD_Plk_4	66	72	PF00069	0.311
MOD_Plk_4	686	692	PF00069	0.370
MOD_Plk_4	717	723	PF00069	0.311
MOD_Plk_4	749	755	PF00069	0.370
MOD_Plk_4	768	774	PF00069	0.463
MOD_Plk_4	810	816	PF00069	0.378
MOD_ProDKin_1	17	23	PF00069	0.630
MOD_ProDKin_1	312	318	PF00069	0.290
MOD_ProDKin_1	634	640	PF00069	0.338
MOD_ProDKin_1	679	685	PF00069	0.314
MOD_ProDKin_1	708	714	PF00069	0.338
MOD_ProDKin_1	743	749	PF00069	0.336
TRG_DiLeu_BaEn_1	686	691	PF01217	0.329
TRG_DiLeu_BaLyEn_6	463	468	PF01217	0.296
TRG_DiLeu_BaLyEn_6	80	85	PF01217	0.329
TRG_ENDOCYTIC_2	138	141	PF00928	0.429
TRG_ENDOCYTIC_2	231	234	PF00928	0.330
TRG_ENDOCYTIC_2	253	256	PF00928	0.330
TRG_ENDOCYTIC_2	263	266	PF00928	0.330
TRG_ENDOCYTIC_2	295	298	PF00928	0.330
TRG_ENDOCYTIC_2	421	424	PF00928	0.278
TRG_ENDOCYTIC_2	445	448	PF00928	0.466
TRG_ENDOCYTIC_2	453	456	PF00928	0.274
TRG_ENDOCYTIC_2	551	554	PF00928	0.742
TRG_ENDOCYTIC_2	57	60	PF00928	0.594
TRG_ENDOCYTIC_2	630	633	PF00928	0.314
TRG_ENDOCYTIC_2	697	700	PF00928	0.293
TRG_ENDOCYTIC_2	722	725	PF00928	0.333
TRG_ENDOCYTIC_2	781	784	PF00928	0.299
TRG_ENDOCYTIC_2	90	93	PF00928	0.303
TRG_ER_diArg_1	237	240	PF00400	0.377
TRG_ER_diArg_1	341	344	PF00400	0.507
TRG_ER_diArg_1	349	352	PF00400	0.478
TRG_ER_diArg_1	357	360	PF00400	0.419
TRG_ER_diArg_1	387	390	PF00400	0.258
TRG_ER_diArg_1	559	561	PF00400	0.618
TRG_NES_CRM1_1	321	336	PF08389	0.381
TRG_Pf-PMV_PEXEL_1	390	395	PF00026	0.479

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IZ72	Bodo saltans	51%	100%
A0A1X0NFU7	Trypanosomatidae	56%	100%
A0A3Q8IHT0	Leishmania donovani	94%	99%
A0A3Q8II34	Leishmania donovani	56%	100%
A0A3Q8ILY7	Leishmania donovani	76%	100%
A0A3Q8IV37	Leishmania donovani	74%	100%
A0A422MX14	Trypanosoma rangeli	56%	100%
A4HFF9	Leishmania braziliensis	78%	99%
A4HMD5	Leishmania braziliensis	72%	100%
A4HMD6	Leishmania braziliensis	54%	100%
A4HMD7	Leishmania braziliensis	66%	100%
A4IB08	Leishmania infantum	94%	99%
A4IB09	Leishmania infantum	77%	100%
A4IB10	Leishmania infantum	56%	100%
C9ZNL1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
C9ZQ40	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
E2RG47	Canis lupus familiaris	31%	100%
E9AET7	Leishmania major	79%	100%
E9AET8	Leishmania major	57%	100%
E9AET9	Leishmania major	69%	99%
E9AHU4	Leishmania infantum	74%	100%
E9B5Z2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	99%
E9B5Z3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	100%
E9B5Z4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	55%	100%
E9B5Z5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	67%	100%
F1PJP5	Canis lupus familiaris	28%	100%
O94335	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
P46975	Caenorhabditis elegans	30%	100%
P46977	Homo sapiens	29%	100%
P46978	Mus musculus	29%	100%
Q2KJI2	Bos taurus	29%	100%
Q3TDQ1	Mus musculus	31%	100%
Q5RCE2	Pongo abelii	29%	100%
Q6F2Z1	Oryza sativa subsp. japonica	29%	100%
Q7XQ88	Oryza sativa subsp. japonica	31%	100%
Q8TCJ2	Homo sapiens	31%	100%
Q93ZY3	Arabidopsis thaliana	32%	100%
Q9FX21	Arabidopsis thaliana	30%	100%
V5BDM6	Trypanosoma cruzi	56%	100%