rRNA adenine N(6)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

rRNA dimethyltransferase, putative

Species:

Leishmania major

UniProt:

E9AEQ2_LEIMA

TriTrypDb:

LmjF.35.0770 , LMJLV39_350013300 , LMJSD75_350012800

Length:

464

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0005737	cytoplasm	2	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AEQ2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEQ2

Function

Biological processes
Term	Name	Level	Count
GO:0000154	rRNA modification	6	2
GO:0001510	RNA methylation	4	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006364	rRNA processing	8	2
GO:0006396	RNA processing	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0016072	rRNA metabolic process	7	2
GO:0031167	rRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000179	rRNA (adenine-N6,N6-)-dimethyltransferase activity	6	11
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0008168	methyltransferase activity	4	11
GO:0008170	N-methyltransferase activity	5	11
GO:0008173	RNA methyltransferase activity	4	11
GO:0008649	rRNA methyltransferase activity	5	11
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	11
GO:0016433	rRNA (adenine) methyltransferase activity	6	11
GO:0016740	transferase activity	2	11
GO:0016741	transferase activity, transferring one-carbon groups	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140102	catalytic activity, acting on a rRNA	4	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	155	157	PF00675	0.530
CLV_NRD_NRD_1	213	215	PF00675	0.560
CLV_PCSK_FUR_1	211	215	PF00082	0.674
CLV_PCSK_KEX2_1	155	157	PF00082	0.524
CLV_PCSK_KEX2_1	213	215	PF00082	0.558
CLV_PCSK_SKI1_1	143	147	PF00082	0.536
CLV_PCSK_SKI1_1	286	290	PF00082	0.377
CLV_PCSK_SKI1_1	383	387	PF00082	0.296
CLV_PCSK_SKI1_1	53	57	PF00082	0.344
CLV_PCSK_SKI1_1	92	96	PF00082	0.291
DEG_ODPH_VHL_1	309	322	PF01847	0.496
DOC_ANK_TNKS_1	28	35	PF00023	0.467
DOC_ANK_TNKS_1	343	350	PF00023	0.562
DOC_CKS1_1	73	78	PF01111	0.534
DOC_CYCLIN_RxL_1	89	96	PF00134	0.482
DOC_MAPK_MEF2A_6	53	60	PF00069	0.482
DOC_MAPK_NFAT4_5	53	61	PF00069	0.482
DOC_PP1_RVXF_1	179	185	PF00149	0.682
DOC_USP7_MATH_1	166	170	PF00917	0.735
DOC_USP7_MATH_1	173	177	PF00917	0.546
DOC_USP7_MATH_1	200	204	PF00917	0.685
DOC_USP7_MATH_1	280	284	PF00917	0.538
DOC_USP7_MATH_1	311	315	PF00917	0.482
DOC_USP7_MATH_1	4	8	PF00917	0.687
DOC_USP7_UBL2_3	36	40	PF12436	0.449
DOC_WW_Pin1_4	156	161	PF00397	0.742
DOC_WW_Pin1_4	238	243	PF00397	0.746
DOC_WW_Pin1_4	456	461	PF00397	0.746
DOC_WW_Pin1_4	72	77	PF00397	0.534
LIG_14-3-3_CanoR_1	181	185	PF00244	0.621
LIG_14-3-3_CanoR_1	319	323	PF00244	0.482
LIG_14-3-3_CanoR_1	331	337	PF00244	0.482
LIG_14-3-3_CanoR_1	383	388	PF00244	0.483
LIG_14-3-3_CanoR_1	70	76	PF00244	0.482
LIG_Actin_WH2_2	129	145	PF00022	0.555
LIG_Actin_WH2_2	369	385	PF00022	0.585
LIG_APCC_ABBA_1	111	116	PF00400	0.562
LIG_BIR_III_4	192	196	PF00653	0.708
LIG_BRCT_BRCA1_1	313	317	PF00533	0.482
LIG_FHA_1	128	134	PF00498	0.562
LIG_FHA_1	270	276	PF00498	0.482
LIG_FHA_1	372	378	PF00498	0.490
LIG_FHA_1	59	65	PF00498	0.471
LIG_FHA_1	89	95	PF00498	0.487
LIG_FHA_2	181	187	PF00498	0.683
LIG_FHA_2	298	304	PF00498	0.463
LIG_FHA_2	333	339	PF00498	0.482
LIG_FHA_2	343	349	PF00498	0.482
LIG_FHA_2	412	418	PF00498	0.482
LIG_FHA_2	72	78	PF00498	0.562
LIG_LIR_Gen_1	314	323	PF02991	0.482
LIG_LIR_Nem_3	144	149	PF02991	0.417
LIG_LIR_Nem_3	314	320	PF02991	0.482
LIG_PTB_Apo_2	120	127	PF02174	0.548
LIG_PTB_Apo_2	262	269	PF02174	0.482
LIG_REV1ctd_RIR_1	325	335	PF16727	0.585
LIG_SH2_CRK	39	43	PF00017	0.567
LIG_SH2_GRB2like	121	124	PF00017	0.548
LIG_SH2_SRC	219	222	PF00017	0.722
LIG_SH2_STAP1	50	54	PF00017	0.496
LIG_SH2_STAT3	402	405	PF00017	0.482
LIG_SH2_STAT5	121	124	PF00017	0.487
LIG_SH2_STAT5	67	70	PF00017	0.562
LIG_SH3_3	14	20	PF00018	0.613
LIG_SH3_3	154	160	PF00018	0.692
LIG_SH3_3	22	28	PF00018	0.484
LIG_SUMO_SIM_anti_2	137	144	PF11976	0.487
LIG_SUMO_SIM_anti_2	318	324	PF11976	0.482
LIG_SUMO_SIM_anti_2	417	422	PF11976	0.585
LIG_SUMO_SIM_par_1	350	355	PF11976	0.562
LIG_SUMO_SIM_par_1	414	419	PF11976	0.496
LIG_TRAF2_1	160	163	PF00917	0.749
LIG_TRAF2_1	441	444	PF00917	0.584
LIG_TRAF2_2	218	223	PF00917	0.648
LIG_TRAF2_2	441	446	PF00917	0.673
LIG_UBA3_1	55	59	PF00899	0.482
MOD_CDK_SPxK_1	72	78	PF00069	0.534
MOD_CK1_1	176	182	PF00069	0.763
MOD_CK1_1	222	228	PF00069	0.764
MOD_CK1_1	318	324	PF00069	0.482
MOD_CK1_1	366	372	PF00069	0.423
MOD_CK1_1	5	11	PF00069	0.674
MOD_CK1_1	66	72	PF00069	0.501
MOD_CK1_1	93	99	PF00069	0.482
MOD_CK2_1	297	303	PF00069	0.474
MOD_CK2_1	342	348	PF00069	0.521
MOD_CK2_1	416	422	PF00069	0.482
MOD_DYRK1A_RPxSP_1	156	160	PF00069	0.735
MOD_GlcNHglycan	143	146	PF01048	0.555
MOD_GlcNHglycan	168	171	PF01048	0.750
MOD_GlcNHglycan	176	179	PF01048	0.629
MOD_GlcNHglycan	202	205	PF01048	0.667
MOD_GlcNHglycan	365	368	PF01048	0.223
MOD_GlcNHglycan	4	7	PF01048	0.664
MOD_GlcNHglycan	460	463	PF01048	0.597
MOD_GSK3_1	176	183	PF00069	0.795
MOD_GSK3_1	311	318	PF00069	0.482
MOD_GSK3_1	383	390	PF00069	0.535
MOD_GSK3_1	68	75	PF00069	0.482
MOD_GSK3_1	86	93	PF00069	0.482
MOD_N-GLC_1	119	124	PF02516	0.348
MOD_NEK2_1	141	146	PF00069	0.608
MOD_NEK2_1	246	251	PF00069	0.718
MOD_NEK2_1	332	337	PF00069	0.518
MOD_NEK2_1	365	370	PF00069	0.581
MOD_NEK2_1	416	421	PF00069	0.550
MOD_NEK2_1	58	63	PF00069	0.472
MOD_NEK2_1	68	73	PF00069	0.473
MOD_NEK2_1	90	95	PF00069	0.482
MOD_PIKK_1	176	182	PF00454	0.758
MOD_PIKK_1	280	286	PF00454	0.562
MOD_PIKK_1	297	303	PF00454	0.378
MOD_PIKK_1	434	440	PF00454	0.502
MOD_PKA_2	180	186	PF00069	0.655
MOD_PKA_2	251	257	PF00069	0.427
MOD_PKA_2	318	324	PF00069	0.482
MOD_PKA_2	69	75	PF00069	0.482
MOD_Plk_1	337	343	PF00069	0.482
MOD_Plk_1	416	422	PF00069	0.562
MOD_Plk_4	318	324	PF00069	0.482
MOD_Plk_4	332	338	PF00069	0.482
MOD_Plk_4	366	372	PF00069	0.494
MOD_Plk_4	411	417	PF00069	0.482
MOD_Plk_4	5	11	PF00069	0.500
MOD_Plk_4	63	69	PF00069	0.562
MOD_ProDKin_1	156	162	PF00069	0.747
MOD_ProDKin_1	238	244	PF00069	0.747
MOD_ProDKin_1	456	462	PF00069	0.692
MOD_ProDKin_1	72	78	PF00069	0.534
MOD_SUMO_for_1	258	261	PF00179	0.482
MOD_SUMO_for_1	30	33	PF00179	0.441
TRG_DiLeu_BaLyEn_6	205	210	PF01217	0.648
TRG_ER_diArg_1	154	156	PF00400	0.511
TRG_ER_diArg_1	210	213	PF00400	0.518
TRG_NES_CRM1_1	398	410	PF08389	0.562
TRG_Pf-PMV_PEXEL_1	208	212	PF00026	0.681
TRG_Pf-PMV_PEXEL_1	78	82	PF00026	0.282

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HXZ3	Leptomonas seymouri	78%	86%
A0A1X0NJH8	Trypanosomatidae	55%	87%
A0A3R7NNC2	Trypanosoma rangeli	59%	97%
A0A3S7X8W9	Leishmania donovani	94%	100%
A4HMA9	Leishmania braziliensis	88%	99%
A4IAX5	Leishmania infantum	94%	100%
D0A280	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	99%
E9B5V7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
V5BN79	Trypanosoma cruzi	61%	97%