Conserved CBS domain protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

AMP-activated protein kinase, gamma regulatory subunit

Species:

Leishmania major

UniProt:

E9AEQ1_LEIMA

TriTrypDb:

LmjF.35.0760 , LMJLV39_350013200 , LMJSD75_350012700

Length:

481

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	2
GO:0031588	nucleotide-activated protein kinase complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	2
GO:0110165	cellular anatomical entity	1	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1902911	protein kinase complex	5	2
GO:1990234	transferase complex	3	2

Expansion

Sequence features

E9AEQ1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEQ1

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	2
GO:0006793	phosphorus metabolic process	3	2
GO:0006796	phosphate-containing compound metabolic process	4	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0007154	cell communication	2	2
GO:0008152	metabolic process	1	2
GO:0009267	cellular response to starvation	4	2
GO:0009605	response to external stimulus	2	2
GO:0009987	cellular process	1	2
GO:0009991	response to extracellular stimulus	3	2
GO:0016310	phosphorylation	5	2
GO:0019538	protein metabolic process	3	2
GO:0031667	response to nutrient levels	4	2
GO:0031668	cellular response to extracellular stimulus	3	2
GO:0031669	cellular response to nutrient levels	4	2
GO:0033554	cellular response to stress	3	2
GO:0036211	protein modification process	4	2
GO:0042149	cellular response to glucose starvation	5	2
GO:0042594	response to starvation	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0050790	regulation of catalytic activity	3	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0065007	biological regulation	1	2
GO:0065009	regulation of molecular function	2	2
GO:0071496	cellular response to external stimulus	3	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	2
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0016208	AMP binding	4	2
GO:0017076	purine nucleotide binding	4	2
GO:0019207	kinase regulator activity	3	2
GO:0019887	protein kinase regulator activity	4	2
GO:0019899	enzyme binding	3	2
GO:0019900	kinase binding	4	2
GO:0019901	protein kinase binding	5	2
GO:0030234	enzyme regulator activity	2	2
GO:0030554	adenyl nucleotide binding	5	2
GO:0032553	ribonucleotide binding	3	2
GO:0032555	purine ribonucleotide binding	4	2
GO:0032559	adenyl ribonucleotide binding	5	2
GO:0036094	small molecule binding	2	2
GO:0043167	ion binding	2	2
GO:0043168	anion binding	3	2
GO:0043169	cation binding	3	2
GO:0097159	organic cyclic compound binding	2	2
GO:0097367	carbohydrate derivative binding	2	2
GO:0098772	molecular function regulator activity	1	2
GO:1901265	nucleoside phosphate binding	3	2
GO:1901363	heterocyclic compound binding	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	232	236	PF00656	0.623
CLV_NRD_NRD_1	180	182	PF00675	0.297
CLV_NRD_NRD_1	355	357	PF00675	0.373
CLV_NRD_NRD_1	447	449	PF00675	0.436
CLV_NRD_NRD_1	93	95	PF00675	0.451
CLV_PCSK_KEX2_1	180	182	PF00082	0.315
CLV_PCSK_KEX2_1	355	357	PF00082	0.420
CLV_PCSK_KEX2_1	446	448	PF00082	0.443
CLV_PCSK_KEX2_1	95	97	PF00082	0.521
CLV_PCSK_PC1ET2_1	95	97	PF00082	0.521
CLV_PCSK_SKI1_1	357	361	PF00082	0.437
CLV_PCSK_SKI1_1	417	421	PF00082	0.394
CLV_PCSK_SKI1_1	438	442	PF00082	0.344
CLV_PCSK_SKI1_1	447	451	PF00082	0.366
DEG_APCC_DBOX_1	397	405	PF00400	0.407
DEG_SPOP_SBC_1	101	105	PF00917	0.391
DEG_SPOP_SBC_1	229	233	PF00917	0.710
DOC_CYCLIN_RxL_1	446	456	PF00134	0.331
DOC_MAPK_gen_1	94	101	PF00069	0.498
DOC_MAPK_MEF2A_6	366	375	PF00069	0.347
DOC_MAPK_MEF2A_6	388	395	PF00069	0.306
DOC_PP2B_LxvP_1	148	151	PF13499	0.706
DOC_PP2B_LxvP_1	41	44	PF13499	0.351
DOC_PP4_FxxP_1	341	344	PF00568	0.371
DOC_USP7_MATH_1	154	158	PF00917	0.316
DOC_USP7_MATH_1	16	20	PF00917	0.510
DOC_USP7_MATH_1	218	222	PF00917	0.637
DOC_USP7_MATH_1	22	26	PF00917	0.416
DOC_USP7_MATH_1	229	233	PF00917	0.682
DOC_USP7_MATH_1	264	268	PF00917	0.776
DOC_USP7_MATH_1	318	322	PF00917	0.602
DOC_USP7_MATH_1	336	340	PF00917	0.537
DOC_USP7_MATH_1	419	423	PF00917	0.438
DOC_USP7_MATH_1	44	48	PF00917	0.347
DOC_WW_Pin1_4	383	388	PF00397	0.306
LIG_14-3-3_CanoR_1	50	58	PF00244	0.355
LIG_14-3-3_CanoR_1	84	93	PF00244	0.482
LIG_APCC_ABBA_1	428	433	PF00400	0.393
LIG_BIR_II_1	1	5	PF00653	0.601
LIG_BIR_III_4	235	239	PF00653	0.634
LIG_BRCT_BRCA1_1	281	285	PF00533	0.709
LIG_BRCT_BRCA1_1	337	341	PF00533	0.491
LIG_BRCT_BRCA1_1	73	77	PF00533	0.409
LIG_CaM_IQ_9	409	425	PF13499	0.514
LIG_Clathr_ClatBox_1	401	405	PF01394	0.410
LIG_Clathr_ClatBox_1	452	456	PF01394	0.353
LIG_EH1_1	176	184	PF00400	0.349
LIG_FHA_1	103	109	PF00498	0.312
LIG_FHA_1	370	376	PF00498	0.360
LIG_FHA_1	377	383	PF00498	0.383
LIG_FHA_1	457	463	PF00498	0.375
LIG_FHA_1	60	66	PF00498	0.379
LIG_FHA_2	101	107	PF00498	0.451
LIG_FHA_2	311	317	PF00498	0.490
LIG_FHA_2	32	38	PF00498	0.470
LIG_FHA_2	413	419	PF00498	0.338
LIG_FHA_2	434	440	PF00498	0.455
LIG_LIR_Apic_2	300	305	PF02991	0.539
LIG_LIR_Apic_2	338	344	PF02991	0.417
LIG_LIR_Gen_1	104	113	PF02991	0.335
LIG_LIR_Gen_1	130	138	PF02991	0.495
LIG_LIR_Gen_1	191	201	PF02991	0.410
LIG_LIR_Gen_1	342	353	PF02991	0.378
LIG_LIR_Gen_1	439	449	PF02991	0.351
LIG_LIR_Gen_1	470	479	PF02991	0.440
LIG_LIR_Gen_1	53	60	PF02991	0.427
LIG_LIR_Nem_3	104	110	PF02991	0.330
LIG_LIR_Nem_3	130	135	PF02991	0.478
LIG_LIR_Nem_3	191	196	PF02991	0.410
LIG_LIR_Nem_3	206	210	PF02991	0.447
LIG_LIR_Nem_3	282	288	PF02991	0.751
LIG_LIR_Nem_3	342	348	PF02991	0.368
LIG_LIR_Nem_3	426	432	PF02991	0.397
LIG_LIR_Nem_3	439	444	PF02991	0.257
LIG_LIR_Nem_3	470	476	PF02991	0.318
LIG_LIR_Nem_3	53	58	PF02991	0.354
LIG_PCNA_yPIPBox_3	461	472	PF02747	0.312
LIG_Pex14_2	341	345	PF04695	0.368
LIG_SH2_CRK	337	341	PF00017	0.350
LIG_SH2_CRK	55	59	PF00017	0.371
LIG_SH2_NCK_1	337	341	PF00017	0.433
LIG_SH2_PTP2	107	110	PF00017	0.265
LIG_SH2_PTP2	302	305	PF00017	0.505
LIG_SH2_PTP2	98	101	PF00017	0.377
LIG_SH2_STAP1	337	341	PF00017	0.473
LIG_SH2_STAP1	410	414	PF00017	0.406
LIG_SH2_STAT3	347	350	PF00017	0.363
LIG_SH2_STAT3	410	413	PF00017	0.475
LIG_SH2_STAT5	107	110	PF00017	0.310
LIG_SH2_STAT5	113	116	PF00017	0.327
LIG_SH2_STAT5	207	210	PF00017	0.313
LIG_SH2_STAT5	302	305	PF00017	0.520
LIG_SH2_STAT5	307	310	PF00017	0.531
LIG_SH2_STAT5	347	350	PF00017	0.380
LIG_SH2_STAT5	473	476	PF00017	0.427
LIG_SH2_STAT5	98	101	PF00017	0.410
LIG_SH3_3	148	154	PF00018	0.645
LIG_SH3_3	300	306	PF00018	0.404
LIG_SH3_3	381	387	PF00018	0.391
LIG_SH3_3	66	72	PF00018	0.411
LIG_SUMO_SIM_anti_2	368	376	PF11976	0.376
LIG_SUMO_SIM_anti_2	61	68	PF11976	0.362
LIG_SUMO_SIM_par_1	181	187	PF11976	0.304
LIG_SUMO_SIM_par_1	371	376	PF11976	0.440
LIG_SUMO_SIM_par_1	391	397	PF11976	0.150
LIG_SUMO_SIM_par_1	61	68	PF11976	0.362
LIG_TRAF2_1	34	37	PF00917	0.432
LIG_TRAF2_1	79	82	PF00917	0.360
LIG_TRFH_1	302	306	PF08558	0.514
LIG_TYR_ITIM	205	210	PF00017	0.445
LIG_TYR_ITSM	103	110	PF00017	0.309
LIG_UBA3_1	182	188	PF00899	0.306
LIG_UBA3_1	211	217	PF00899	0.495
LIG_UBA3_1	359	366	PF00899	0.355
LIG_WRC_WIRS_1	45	50	PF05994	0.347
MOD_CDK_SPK_2	383	388	PF00069	0.306
MOD_CK1_1	157	163	PF00069	0.272
MOD_CK1_1	255	261	PF00069	0.710
MOD_CK1_1	267	273	PF00069	0.768
MOD_CK1_1	310	316	PF00069	0.484
MOD_CK1_1	422	428	PF00069	0.474
MOD_CK2_1	22	28	PF00069	0.505
MOD_CK2_1	31	37	PF00069	0.384
MOD_CK2_1	310	316	PF00069	0.463
MOD_CK2_1	383	389	PF00069	0.379
MOD_CK2_1	412	418	PF00069	0.365
MOD_CK2_1	433	439	PF00069	0.480
MOD_GlcNHglycan	1	4	PF01048	0.591
MOD_GlcNHglycan	13	16	PF01048	0.447
MOD_GlcNHglycan	320	323	PF01048	0.595
MOD_GSK3_1	11	18	PF00069	0.495
MOD_GSK3_1	153	160	PF00069	0.394
MOD_GSK3_1	218	225	PF00069	0.473
MOD_GSK3_1	264	271	PF00069	0.641
MOD_GSK3_1	365	372	PF00069	0.384
MOD_GSK3_1	80	87	PF00069	0.507
MOD_N-GLC_1	229	234	PF02516	0.709
MOD_N-GLC_1	250	255	PF02516	0.672
MOD_N-GLC_1	258	263	PF02516	0.656
MOD_N-GLC_1	274	279	PF02516	0.772
MOD_N-GLC_1	50	55	PF02516	0.480
MOD_NEK2_1	252	257	PF00069	0.733
MOD_NEK2_1	58	63	PF00069	0.391
MOD_NEK2_2	336	341	PF00069	0.431
MOD_PIKK_1	310	316	PF00454	0.523
MOD_PIKK_1	409	415	PF00454	0.488
MOD_PIKK_1	59	65	PF00454	0.377
MOD_PKA_2	31	37	PF00069	0.547
MOD_Plk_1	163	169	PF00069	0.322
MOD_Plk_1	191	197	PF00069	0.322
MOD_Plk_1	250	256	PF00069	0.721
MOD_Plk_1	267	273	PF00069	0.700
MOD_Plk_1	50	56	PF00069	0.468
MOD_Plk_2-3	433	439	PF00069	0.476
MOD_Plk_4	154	160	PF00069	0.330
MOD_Plk_4	22	28	PF00069	0.618
MOD_Plk_4	307	313	PF00069	0.504
MOD_Plk_4	336	342	PF00069	0.484
MOD_Plk_4	369	375	PF00069	0.426
MOD_Plk_4	44	50	PF00069	0.469
MOD_Plk_4	6	12	PF00069	0.534
MOD_ProDKin_1	383	389	PF00069	0.306
TRG_AP2beta_CARGO_1	439	448	PF09066	0.329
TRG_DiLeu_BaEn_1	191	196	PF01217	0.410
TRG_DiLeu_BaEn_1	369	374	PF01217	0.322
TRG_DiLeu_BaEn_2	469	475	PF01217	0.301
TRG_DiLeu_BaEn_4	36	42	PF01217	0.370
TRG_DiLeu_BaLyEn_6	458	463	PF01217	0.484
TRG_ENDOCYTIC_2	107	110	PF00928	0.265
TRG_ENDOCYTIC_2	207	210	PF00928	0.444
TRG_ENDOCYTIC_2	337	340	PF00928	0.391
TRG_ENDOCYTIC_2	473	476	PF00928	0.423
TRG_ENDOCYTIC_2	55	58	PF00928	0.348
TRG_ENDOCYTIC_2	98	101	PF00928	0.384
TRG_ER_diArg_1	179	181	PF00400	0.303
TRG_ER_diArg_1	445	448	PF00400	0.351
TRG_ER_diArg_1	93	96	PF00400	0.457
TRG_NES_CRM1_1	56	68	PF08389	0.436
TRG_NLS_MonoExtN_4	93	98	PF00514	0.451
TRG_Pf-PMV_PEXEL_1	461	465	PF00026	0.455

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCX9	Leptomonas seymouri	75%	99%
A0A1X0NKG9	Trypanosomatidae	44%	90%
A0A3Q8ILV4	Leishmania donovani	98%	100%
A0A422NI77	Trypanosoma rangeli	48%	96%
A4HMA8	Leishmania braziliensis	84%	98%
A4IAX4	Leishmania infantum	97%	100%
D0A281	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	96%
E9B5V6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
V5BSS4	Trypanosoma cruzi	49%	96%