Pyruvate kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Pyruvate kinase

Gene product:

pyruvate kinase

Species:

Leishmania major

UniProt:

E9AEH9_LEIMA

TriTrypDb:

LmjF.35.0030 , LMJLV39_350005100 , LMJSD75_350005300

Length:

524

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 1
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 24

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 17
NetGPI	no	yes: 0, no: 17

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

E9AEH9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEH9

Function

Biological processes
Term	Name	Level	Count
GO:0005975	carbohydrate metabolic process	3	3
GO:0006082	organic acid metabolic process	3	3
GO:0006090	pyruvate metabolic process	7	3
GO:0006091	generation of precursor metabolites and energy	3	3
GO:0006096	glycolytic process	5	3
GO:0006139	nucleobase-containing compound metabolic process	3	3
GO:0006163	purine nucleotide metabolic process	5	3
GO:0006165	obsolete nucleoside diphosphate phosphorylation	6	3
GO:0006725	cellular aromatic compound metabolic process	3	3
GO:0006753	nucleoside phosphate metabolic process	4	3
GO:0006757	obsolete ATP generation from ADP	4	3
GO:0006793	phosphorus metabolic process	3	3
GO:0006796	phosphate-containing compound metabolic process	4	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0009056	catabolic process	2	3
GO:0009117	nucleotide metabolic process	5	3
GO:0009132	nucleoside diphosphate metabolic process	5	3
GO:0009135	purine nucleoside diphosphate metabolic process	6	3
GO:0009141	nucleoside triphosphate metabolic process	5	3
GO:0009144	purine nucleoside triphosphate metabolic process	6	3
GO:0009150	purine ribonucleotide metabolic process	6	3
GO:0009179	purine ribonucleoside diphosphate metabolic process	7	3
GO:0009185	ribonucleoside diphosphate metabolic process	6	3
GO:0009199	ribonucleoside triphosphate metabolic process	6	3
GO:0009205	purine ribonucleoside triphosphate metabolic process	7	3
GO:0009259	ribonucleotide metabolic process	5	3
GO:0009987	cellular process	1	3
GO:0016052	carbohydrate catabolic process	4	3
GO:0016310	phosphorylation	5	3
GO:0019637	organophosphate metabolic process	3	3
GO:0019693	ribose phosphate metabolic process	4	3
GO:0019752	carboxylic acid metabolic process	5	3
GO:0032787	monocarboxylic acid metabolic process	6	3
GO:0034641	cellular nitrogen compound metabolic process	3	3
GO:0043436	oxoacid metabolic process	4	3
GO:0044237	cellular metabolic process	2	3
GO:0044238	primary metabolic process	2	3
GO:0044281	small molecule metabolic process	2	3
GO:0046031	ADP metabolic process	7	3
GO:0046034	ATP metabolic process	7	3
GO:0046483	heterocycle metabolic process	3	3
GO:0046939	obsolete nucleotide phosphorylation	6	3
GO:0055086	nucleobase-containing small molecule metabolic process	3	3
GO:0071704	organic substance metabolic process	2	3
GO:0072521	purine-containing compound metabolic process	4	3
GO:1901135	carbohydrate derivative metabolic process	3	3
GO:1901360	organic cyclic compound metabolic process	3	3
GO:1901564	organonitrogen compound metabolic process	3	3
GO:1901575	organic substance catabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	18
GO:0000287	magnesium ion binding	5	18
GO:0003824	catalytic activity	1	18
GO:0004743	pyruvate kinase activity	5	18
GO:0005488	binding	1	18
GO:0005524	ATP binding	5	18
GO:0016301	kinase activity	4	18
GO:0016740	transferase activity	2	18
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	18
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	18
GO:0017076	purine nucleotide binding	4	18
GO:0030554	adenyl nucleotide binding	5	18
GO:0030955	potassium ion binding	6	18
GO:0031420	alkali metal ion binding	5	18
GO:0032553	ribonucleotide binding	3	18
GO:0032555	purine ribonucleotide binding	4	18
GO:0032559	adenyl ribonucleotide binding	5	18
GO:0035639	purine ribonucleoside triphosphate binding	4	18
GO:0036094	small molecule binding	2	18
GO:0043167	ion binding	2	18
GO:0043168	anion binding	3	18
GO:0043169	cation binding	3	18
GO:0043531	ADP binding	4	3
GO:0046872	metal ion binding	4	18
GO:0097159	organic cyclic compound binding	2	18
GO:0097367	carbohydrate derivative binding	2	18
GO:1901265	nucleoside phosphate binding	3	18
GO:1901363	heterocyclic compound binding	2	18

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	209	213	PF00656	0.495
CLV_NRD_NRD_1	178	180	PF00675	0.366
CLV_PCSK_SKI1_1	179	183	PF00082	0.299
CLV_PCSK_SKI1_1	59	63	PF00082	0.358
DOC_MAPK_gen_1	348	355	PF00069	0.513
DOC_MAPK_gen_1	409	419	PF00069	0.477
DOC_MAPK_gen_1	89	99	PF00069	0.556
DOC_MAPK_MEF2A_6	218	225	PF00069	0.474
DOC_MAPK_MEF2A_6	348	355	PF00069	0.526
DOC_MAPK_MEF2A_6	369	377	PF00069	0.526
DOC_MAPK_MEF2A_6	412	419	PF00069	0.459
DOC_PP2B_LxvP_1	481	484	PF13499	0.447
DOC_USP7_MATH_1	143	147	PF00917	0.444
DOC_USP7_MATH_1	267	271	PF00917	0.474
DOC_USP7_MATH_1	335	339	PF00917	0.478
DOC_USP7_MATH_1	340	344	PF00917	0.482
DOC_WW_Pin1_4	460	465	PF00397	0.448
LIG_14-3-3_CanoR_1	380	389	PF00244	0.482
LIG_14-3-3_CanoR_1	5	15	PF00244	0.468
LIG_BIR_II_1	1	5	PF00653	0.449
LIG_BRCT_BRCA1_1	394	398	PF00533	0.580
LIG_FHA_1	250	256	PF00498	0.479
LIG_FHA_1	376	382	PF00498	0.497
LIG_FHA_1	388	394	PF00498	0.460
LIG_FHA_1	447	453	PF00498	0.434
LIG_FHA_1	60	66	PF00498	0.512
LIG_FHA_2	124	130	PF00498	0.561
LIG_FHA_2	263	269	PF00498	0.474
LIG_Integrin_RGD_1	218	220	PF01839	0.274
LIG_KLC1_Yacidic_2	78	83	PF13176	0.474
LIG_LIR_Gen_1	162	172	PF02991	0.474
LIG_LIR_Gen_1	315	322	PF02991	0.537
LIG_LIR_Gen_1	83	91	PF02991	0.548
LIG_LIR_Gen_1	95	104	PF02991	0.576
LIG_LIR_Nem_3	162	168	PF02991	0.474
LIG_LIR_Nem_3	315	319	PF02991	0.536
LIG_LIR_Nem_3	78	84	PF02991	0.495
LIG_LIR_Nem_3	95	99	PF02991	0.474
LIG_LRP6_Inhibitor_1	317	323	PF00058	0.374
LIG_MLH1_MIPbox_1	394	398	PF16413	0.576
LIG_PCNA_yPIPBox_3	304	317	PF02747	0.576
LIG_PTB_Apo_2	271	278	PF02174	0.509
LIG_SH2_CRK	84	88	PF00017	0.558
LIG_SH2_GRB2like	84	87	PF00017	0.558
LIG_SH2_PTP2	96	99	PF00017	0.576
LIG_SH2_SRC	96	99	PF00017	0.576
LIG_SH2_STAP1	345	349	PF00017	0.509
LIG_SH2_STAP1	475	479	PF00017	0.448
LIG_SH2_STAT5	316	319	PF00017	0.493
LIG_SH2_STAT5	64	67	PF00017	0.546
LIG_SH2_STAT5	81	84	PF00017	0.408
LIG_SH2_STAT5	96	99	PF00017	0.474
LIG_SH3_1	367	373	PF00018	0.474
LIG_SH3_3	130	136	PF00018	0.495
LIG_SH3_3	137	143	PF00018	0.458
LIG_SH3_3	220	226	PF00018	0.483
LIG_SH3_3	367	373	PF00018	0.474
LIG_SH3_3	87	93	PF00018	0.565
LIG_SUMO_SIM_anti_2	100	107	PF11976	0.576
LIG_SUMO_SIM_anti_2	188	193	PF11976	0.495
LIG_SUMO_SIM_par_1	351	356	PF11976	0.466
LIG_TRAF2_1	331	334	PF00917	0.483
LIG_TYR_ITIM	82	87	PF00017	0.483
LIG_UBA3_1	189	194	PF00899	0.495
LIG_UBA3_1	434	441	PF00899	0.476
LIG_WRC_WIRS_1	165	170	PF05994	0.474
MOD_CK1_1	286	292	PF00069	0.474
MOD_CK1_1	9	15	PF00069	0.482
MOD_CK2_1	203	209	PF00069	0.535
MOD_CK2_1	262	268	PF00069	0.474
MOD_CK2_1	328	334	PF00069	0.451
MOD_CK2_1	340	346	PF00069	0.438
MOD_CK2_1	462	468	PF00069	0.445
MOD_GlcNHglycan	145	148	PF01048	0.356
MOD_GlcNHglycan	170	173	PF01048	0.376
MOD_GlcNHglycan	285	288	PF01048	0.274
MOD_GlcNHglycan	310	313	PF01048	0.274
MOD_GlcNHglycan	330	333	PF01048	0.444
MOD_GlcNHglycan	338	341	PF01048	0.255
MOD_GlcNHglycan	360	363	PF01048	0.307
MOD_GlcNHglycan	426	430	PF01048	0.227
MOD_GSK3_1	113	120	PF00069	0.576
MOD_GSK3_1	164	171	PF00069	0.483
MOD_GSK3_1	336	343	PF00069	0.479
MOD_GSK3_1	376	383	PF00069	0.479
MOD_GSK3_1	458	465	PF00069	0.443
MOD_GSK3_1	509	516	PF00069	0.478
MOD_GSK3_1	59	66	PF00069	0.526
MOD_N-GLC_1	387	392	PF02516	0.309
MOD_N-GLC_1	6	11	PF02516	0.282
MOD_N-GLC_1	85	90	PF02516	0.293
MOD_NEK2_1	117	122	PF00069	0.579
MOD_NEK2_1	254	259	PF00069	0.474
MOD_NEK2_1	318	323	PF00069	0.399
MOD_NEK2_1	355	360	PF00069	0.479
MOD_NEK2_1	387	392	PF00069	0.479
MOD_NEK2_1	419	424	PF00069	0.538
MOD_NEK2_1	446	451	PF00069	0.331
MOD_NEK2_1	466	471	PF00069	0.446
MOD_NEK2_1	473	478	PF00069	0.448
MOD_NEK2_1	6	11	PF00069	0.500
MOD_NEK2_2	340	345	PF00069	0.509
MOD_NEK2_2	392	397	PF00069	0.576
MOD_PIKK_1	249	255	PF00454	0.474
MOD_PIKK_1	387	393	PF00454	0.538
MOD_PIKK_1	513	519	PF00454	0.470
MOD_PKA_2	262	268	PF00069	0.487
MOD_PKA_2	446	452	PF00069	0.350
MOD_Plk_1	113	119	PF00069	0.474
MOD_Plk_4	164	170	PF00069	0.474
MOD_Plk_4	203	209	PF00069	0.526
MOD_Plk_4	340	346	PF00069	0.509
MOD_Plk_4	392	398	PF00069	0.504
MOD_Plk_4	446	452	PF00069	0.397
MOD_Plk_4	466	472	PF00069	0.493
MOD_Plk_4	92	98	PF00069	0.484
MOD_ProDKin_1	460	466	PF00069	0.450
MOD_SUMO_rev_2	343	349	PF00179	0.529
TRG_DiLeu_BaEn_3	209	215	PF01217	0.576
TRG_ENDOCYTIC_2	316	319	PF00928	0.482
TRG_ENDOCYTIC_2	84	87	PF00928	0.487
TRG_ENDOCYTIC_2	96	99	PF00928	0.474

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBX8	Leptomonas seymouri	88%	100%
A0A0S4JHN6	Bodo saltans	65%	100%
A0A1X0P7Q1	Trypanosomatidae	78%	100%
A0A3Q8IES7	Leishmania donovani	98%	100%
A0A3S7X8M0	Leishmania donovani	95%	100%
A0A422NPT0	Trypanosoma rangeli	75%	100%
A4HM36	Leishmania braziliensis	93%	100%
A4HM39	Leishmania braziliensis	93%	100%
A4HM41	Leishmania braziliensis	92%	100%
A4IAQ1	Leishmania infantum	98%	100%
A4IAQ4	Leishmania infantum	96%	100%
B0B7Q0	Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B)	40%	100%
B8BJ39	Oryza sativa subsp. indica	35%	99%
B8BM17	Oryza sativa subsp. indica	35%	99%
C9ZZZ0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	74%	100%
C9ZZZ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	75%	100%
E9AEI0	Leishmania major	99%	100%
E9B5P1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
O05118	Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)	36%	100%
O08309	Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)	43%	100%
O44006	Eimeria tenella	46%	99%
O51323	Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)	38%	100%
O62619	Drosophila melanogaster	52%	98%
O65595	Arabidopsis thaliana	49%	100%
O94122	Agaricus bisporus	51%	98%
P00548	Gallus gallus	50%	99%
P00549	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	50%	100%
P0AD61	Escherichia coli (strain K12)	46%	100%
P0AD62	Escherichia coli O157:H7	46%	100%
P0CE21	Chlamydia trachomatis (strain D/UW-3/Cx)	40%	100%
P11974	Oryctolagus cuniculus	50%	99%
P11979	Felis catus	50%	99%
P11980	Rattus norvegicus	51%	99%
P12928	Rattus norvegicus	47%	91%
P14618	Homo sapiens	51%	99%
P21599	Escherichia coli (strain K12)	36%	100%
P22200	Solanum tuberosum	47%	100%
P22360	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	50%	100%
P30613	Homo sapiens	48%	91%
P30614	Yarrowia lipolytica (strain CLIB 122 / E 150)	51%	100%
P30615	Trypanosoma brucei brucei	75%	100%
P30616	Trypanosoma brucei brucei	74%	100%
P31865	Hypocrea jecorina	51%	97%
P32044	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	33%	96%
P34038	Lactobacillus delbrueckii subsp. bulgaricus	40%	89%
P43924	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	36%	100%
P46614	Candida albicans (strain SC5314 / ATCC MYA-2876)	53%	100%
P51181	Bacillus licheniformis	47%	90%
P51182	Sporosarcina psychrophila	45%	89%
P52480	Mus musculus	51%	99%
P52489	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	49%	100%
P53657	Mus musculus	46%	91%
P55964	Ricinus communis	36%	100%
P57404	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	34%	100%
P70789	Agrobacterium vitis	39%	100%
P73534	Synechocystis sp. (strain PCC 6803 / Kazusa)	41%	89%
P77983	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	44%	100%
P80885	Bacillus subtilis (strain 168)	47%	90%
P94939	Mycobacterium intracellulare	37%	100%
P9WKE4	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	38%	100%
P9WKE5	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	38%	100%
Q02499	Geobacillus stearothermophilus	45%	89%
Q07637	Lactococcus lactis subsp. lactis (strain IL1403)	40%	100%
Q10208	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	55%	100%
Q12669	Aspergillus niger	51%	100%
Q27686	Leishmania mexicana	95%	100%
Q27788	Trypanoplasma borreli	59%	100%
Q29536	Canis lupus familiaris	48%	91%
Q2FG40	Staphylococcus aureus (strain USA300)	45%	90%
Q2FXM9	Staphylococcus aureus (strain NCTC 8325 / PS 47)	45%	90%
Q2QXR8	Oryza sativa subsp. japonica	35%	99%
Q2RAK2	Oryza sativa subsp. japonica	35%	99%
Q2YTE3	Staphylococcus aureus (strain bovine RF122 / ET3-1)	45%	90%
Q40545	Nicotiana tabacum	32%	88%
Q40546	Nicotiana tabacum	36%	93%
Q42806	Glycine max	47%	100%
Q42954	Nicotiana tabacum	44%	100%
Q43117	Ricinus communis	32%	90%
Q44473	Agrobacterium vitis	38%	100%
Q46078	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	39%	100%
Q46289	Clostridium perfringens (strain 13 / Type A)	43%	100%
Q49YC7	Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)	45%	89%
Q4L739	Staphylococcus haemolyticus (strain JCSC1435)	44%	89%
Q54RF5	Dictyostelium discoideum	51%	100%
Q55863	Synechocystis sp. (strain PCC 6803 / Kazusa)	39%	100%
Q56301	Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)	37%	100%
Q57572	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	34%	100%
Q5HF76	Staphylococcus aureus (strain COL)	45%	90%
Q5HNK7	Staphylococcus epidermidis (strain ATCC 35984 / RP62A)	45%	90%
Q5NVN0	Pongo abelii	50%	99%
Q6BS75	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	49%	100%
Q6FIS9	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	52%	100%
Q6FV12	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	51%	100%
Q6G8M9	Staphylococcus aureus (strain MSSA476)	45%	90%
Q6GG09	Staphylococcus aureus (strain MRSA252)	45%	90%
Q759A9	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	50%	100%
Q7A0N4	Staphylococcus aureus (strain MW2)	45%	90%
Q7A559	Staphylococcus aureus (strain N315)	45%	90%
Q7RVA8	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	50%	99%
Q875M9	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	51%	100%
Q875S4	Lachancea kluyveri (strain ATCC 58438 / CBS 3082 / BCRC 21498 / NBRC 1685 / JCM 7257 / NCYC 543 / NRRL Y-12651)	51%	100%
Q875Z9	Naumovozyma castellii (strain ATCC 76901 / BCRC 22586 / CBS 4309 / NBRC 1992 / NRRL Y-12630)	51%	100%
Q89AI8	Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)	33%	100%
Q8CS69	Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)	45%	90%
Q8FP04	Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)	40%	100%
Q8K9M3	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	34%	100%
Q8SQP0	Encephalitozoon cuniculi (strain GB-M1)	35%	100%
Q8Z6K2	Salmonella typhi	44%	100%
Q8ZNW0	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	37%	100%
Q8ZYE0	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	26%	100%
Q92122	Xenopus laevis	50%	99%
Q93Z53	Arabidopsis thaliana	36%	92%
Q99TG5	Staphylococcus aureus (strain Mu50 / ATCC 700699)	45%	90%
Q9FLW9	Arabidopsis thaliana	37%	91%
Q9LIK0	Arabidopsis thaliana	33%	88%
Q9PK61	Chlamydia muridarum (strain MoPn / Nigg)	41%	100%
Q9WY51	Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)	34%	100%
Q9YEU2	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	34%	100%
Q9Z984	Chlamydia pneumoniae	38%	100%
V5C1M1	Trypanosoma cruzi	75%	100%