Putative serine/threonine protein kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Wee1-like protein kinase, putative

Species:

Leishmania major

UniProt:

E9AED4_LEIMA

TriTrypDb:

LmjF.29.2720 , LMJLV39_290035500 , LMJSD75_290035800

Length:

323

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AED4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AED4

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	9
GO:0006793	phosphorus metabolic process	3	9
GO:0006796	phosphate-containing compound metabolic process	4	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009987	cellular process	1	9
GO:0016310	phosphorylation	5	9
GO:0019538	protein metabolic process	3	9
GO:0036211	protein modification process	4	9
GO:0043170	macromolecule metabolic process	3	9
GO:0043412	macromolecule modification	4	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0071704	organic substance metabolic process	2	9
GO:1901564	organonitrogen compound metabolic process	3	9

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004672	protein kinase activity	3	11
GO:0004674	protein serine/threonine kinase activity	4	5
GO:0004713	protein tyrosine kinase activity	4	2
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016301	kinase activity	4	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	308	312	PF00656	0.568
CLV_NRD_NRD_1	204	206	PF00675	0.387
CLV_NRD_NRD_1	271	273	PF00675	0.420
CLV_PCSK_KEX2_1	204	206	PF00082	0.357
CLV_PCSK_KEX2_1	271	273	PF00082	0.420
CLV_PCSK_KEX2_1	68	70	PF00082	0.355
CLV_PCSK_PC1ET2_1	68	70	PF00082	0.355
CLV_PCSK_SKI1_1	106	110	PF00082	0.368
DOC_ANK_TNKS_1	285	292	PF00023	0.407
DOC_PP2B_LxvP_1	122	125	PF13499	0.459
DOC_USP7_MATH_1	18	22	PF00917	0.691
DOC_USP7_MATH_1	86	90	PF00917	0.426
DOC_WW_Pin1_4	205	210	PF00397	0.371
DOC_WW_Pin1_4	251	256	PF00397	0.377
DOC_WW_Pin1_4	317	322	PF00397	0.653
LIG_14-3-3_CanoR_1	17	27	PF00244	0.646
LIG_BIR_II_1	1	5	PF00653	0.734
LIG_BRCT_BRCA1_1	253	257	PF00533	0.426
LIG_deltaCOP1_diTrp_1	100	109	PF00928	0.357
LIG_eIF4E_1	206	212	PF01652	0.426
LIG_FHA_1	130	136	PF00498	0.459
LIG_FHA_1	160	166	PF00498	0.350
LIG_FHA_1	256	262	PF00498	0.450
LIG_FHA_2	235	241	PF00498	0.427
LIG_FHA_2	280	286	PF00498	0.519
LIG_Integrin_isoDGR_2	184	186	PF01839	0.226
LIG_LIR_Apic_2	203	209	PF02991	0.314
LIG_LIR_Gen_1	254	265	PF02991	0.426
LIG_LIR_Gen_1	274	284	PF02991	0.129
LIG_LIR_Nem_3	192	197	PF02991	0.426
LIG_LIR_Nem_3	254	260	PF02991	0.359
LIG_LIR_Nem_3	274	279	PF02991	0.129
LIG_LIR_Nem_3	44	50	PF02991	0.318
LIG_SH2_NCK_1	253	257	PF00017	0.403
LIG_SH2_STAT5	98	101	PF00017	0.355
LIG_SH3_3	215	221	PF00018	0.390
LIG_Sin3_3	257	264	PF02671	0.459
LIG_SUMO_SIM_par_1	25	30	PF11976	0.614
MOD_CDK_SPK_2	317	322	PF00069	0.614
MOD_CK1_1	129	135	PF00069	0.436
MOD_CK1_1	2	8	PF00069	0.671
MOD_CK1_1	243	249	PF00069	0.385
MOD_CK1_1	25	31	PF00069	0.584
MOD_CK1_1	43	49	PF00069	0.252
MOD_CK2_1	234	240	PF00069	0.383
MOD_CK2_1	279	285	PF00069	0.488
MOD_GlcNHglycan	245	248	PF01048	0.351
MOD_GlcNHglycan	273	276	PF01048	0.426
MOD_GlcNHglycan	4	7	PF01048	0.731
MOD_GSK3_1	18	25	PF00069	0.576
MOD_GSK3_1	236	243	PF00069	0.353
MOD_GSK3_1	251	258	PF00069	0.360
MOD_GSK3_1	30	37	PF00069	0.303
MOD_GSK3_1	316	323	PF00069	0.699
MOD_N-GLC_1	243	248	PF02516	0.386
MOD_NEK2_1	135	140	PF00069	0.345
MOD_NEK2_1	19	24	PF00069	0.640
MOD_NEK2_1	260	265	PF00069	0.426
MOD_PIKK_1	19	25	PF00454	0.683
MOD_PKA_1	271	277	PF00069	0.420
MOD_PKA_2	185	191	PF00069	0.226
MOD_PKA_2	271	277	PF00069	0.387
MOD_PKA_2	30	36	PF00069	0.567
MOD_Plk_4	126	132	PF00069	0.424
MOD_Plk_4	166	172	PF00069	0.426
MOD_Plk_4	260	266	PF00069	0.435
MOD_ProDKin_1	205	211	PF00069	0.371
MOD_ProDKin_1	251	257	PF00069	0.377
MOD_ProDKin_1	317	323	PF00069	0.658
MOD_SUMO_for_1	155	158	PF00179	0.357
TRG_DiLeu_BaLyEn_6	283	288	PF01217	0.562
TRG_DiLeu_BaLyEn_6	289	294	PF01217	0.456
TRG_ER_diArg_1	204	206	PF00400	0.387
TRG_ER_diArg_1	271	273	PF00400	0.420
TRG_Pf-PMV_PEXEL_1	163	168	PF00026	0.419
TRG_Pf-PMV_PEXEL_1	286	290	PF00026	0.587
TRG_Pf-PMV_PEXEL_1	292	297	PF00026	0.552

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2X9	Leptomonas seymouri	25%	99%
A0A0N0P3N5	Leptomonas seymouri	24%	75%
A0A0N0P953	Leptomonas seymouri	26%	67%
A0A0N1HT76	Leptomonas seymouri	24%	72%
A0A0N1I0G1	Leptomonas seymouri	29%	100%
A0A0N1I3G0	Leptomonas seymouri	29%	80%
A0A0N1I4Y7	Leptomonas seymouri	26%	96%
A0A0N1I6T8	Leptomonas seymouri	27%	100%
A0A0N1IHY4	Leptomonas seymouri	24%	79%
A0A0N1IIU9	Leptomonas seymouri	30%	83%
A0A0N1IM47	Leptomonas seymouri	27%	88%
A0A0N1PC01	Leptomonas seymouri	67%	98%
A0A0N1PC31	Leptomonas seymouri	27%	83%
A0A0N1PD05	Leptomonas seymouri	25%	72%
A0A0N1PDW7	Leptomonas seymouri	28%	82%
A0A0S4IHW4	Bodo saltans	23%	72%
A0A0S4ISS4	Bodo saltans	26%	100%
A0A0S4IVQ6	Bodo saltans	26%	69%
A0A0S4IX86	Bodo saltans	24%	75%
A0A0S4J3Y5	Bodo saltans	26%	75%
A0A0S4J4B3	Bodo saltans	25%	72%
A0A0S4JCG2	Bodo saltans	26%	72%
A0A0S4JF22	Bodo saltans	24%	90%
A0A0S4JHA7	Bodo saltans	29%	70%
A0A0S4JU73	Bodo saltans	27%	68%
A0A0S4JUJ2	Bodo saltans	27%	100%
A0A0S4JVD7	Bodo saltans	26%	74%
A0A0S4KEA4	Bodo saltans	35%	80%
A0A0S4KMV7	Bodo saltans	28%	100%
A0A0S4KN74	Bodo saltans	27%	99%
A0A1X0NIA0	Trypanosomatidae	25%	75%
A0A1X0NLC8	Trypanosomatidae	29%	68%
A0A1X0NQ63	Trypanosomatidae	25%	100%
A0A1X0NRZ9	Trypanosomatidae	25%	88%
A0A1X0NTR6	Trypanosomatidae	26%	92%
A0A1X0NY93	Trypanosomatidae	26%	82%
A0A1X0P1A2	Trypanosomatidae	23%	70%
A0A1X0P3X8	Trypanosomatidae	25%	99%
A0A1X0P527	Trypanosomatidae	26%	73%
A0A1X0P6E7	Trypanosomatidae	26%	82%
A0A1X0P6V1	Trypanosomatidae	24%	100%
A0A1X0P735	Trypanosomatidae	26%	100%
A0A1X0P919	Trypanosomatidae	26%	87%
A0A1X0P9V2	Trypanosomatidae	47%	89%
A0A3Q8IB74	Leishmania donovani	24%	79%
A0A3Q8IBT5	Leishmania donovani	26%	100%
A0A3Q8IC87	Leishmania donovani	26%	87%
A0A3Q8IFW0	Leishmania donovani	24%	75%
A0A3Q8INQ4	Leishmania donovani	26%	83%
A0A3Q8IPR6	Leishmania donovani	25%	99%
A0A3R7KCZ4	Trypanosoma rangeli	25%	80%
A0A3R7KRP1	Trypanosoma rangeli	28%	68%
A0A3R7M5C7	Trypanosoma rangeli	26%	100%
A0A3R7MHW5	Trypanosoma rangeli	26%	81%
A0A3R7N4D8	Trypanosoma rangeli	25%	80%
A0A3R7NKI8	Trypanosoma rangeli	24%	100%
A0A3S5H606	Leishmania donovani	27%	100%
A0A3S5H789	Leishmania donovani	25%	84%
A0A3S5IRC3	Trypanosoma rangeli	25%	86%
A0A3S7WP41	Leishmania donovani	27%	100%
A0A3S7WYP4	Leishmania donovani	25%	92%
A0A3S7X2W9	Leishmania donovani	94%	100%
A0A3S7X6T8	Leishmania donovani	22%	72%
A0A3S7X7Y2	Leishmania donovani	25%	84%
A0A422NEU6	Trypanosoma rangeli	44%	95%
A0A422P0J2	Trypanosoma rangeli	26%	96%
A0A422P0N6	Trypanosoma rangeli	28%	100%
A1CL96	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	28%	76%
A1D624	Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)	28%	76%
A4H4A1	Leishmania braziliensis	27%	100%
A4H539	Leishmania braziliensis	27%	100%
A4H9X5	Leishmania braziliensis	25%	79%
A4HAS1	Leishmania braziliensis	25%	84%
A4HCD7	Leishmania braziliensis	26%	76%
A4HDU3	Leishmania braziliensis	24%	92%
A4HED7	Leishmania braziliensis	26%	83%
A4HFI2	Leishmania braziliensis	24%	98%
A4HHS9	Leishmania braziliensis	81%	100%
A4HJW2	Leishmania braziliensis	24%	75%
A4HLR0	Leishmania braziliensis	24%	72%
A4HSH7	Leishmania infantum	27%	100%
A4HTB4	Leishmania infantum	27%	100%
A4HYX6	Leishmania infantum	25%	84%
A4HZV1	Leishmania infantum	26%	87%
A4I140	Leishmania infantum	25%	92%
A4I1T4	Leishmania infantum	26%	83%
A4I4Y0	Leishmania infantum	94%	100%
A4I7C4	Leishmania infantum	24%	75%
A4I960	Leishmania infantum	22%	72%
A4I9Y5	Leishmania infantum	25%	84%
A4K2M3	Papio anubis	26%	66%
A4K2P5	Colobus guereza	26%	66%
A4K2Q5	Otolemur garnettii	26%	66%
A4K2S1	Lemur catta	26%	66%
A4K2T0	Macaca mulatta	26%	66%
A4K2W5	Aotus nancymaae	26%	66%
A4K2Y1	Chlorocebus aethiops	26%	66%
A9RWC9	Physcomitrium patens	25%	92%
A9S5R3	Physcomitrium patens	25%	93%
B0LT89	Rattus norvegicus	25%	75%
B0Y8W7	Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163)	26%	92%
C9ZKZ8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	23%	68%
C9ZL78	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	77%
C9ZN29	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	23%	67%
C9ZSI7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	100%
C9ZUK8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
C9ZWI1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	75%
D0A2R4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	85%
D0A3L9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	92%
D0A5Q8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	99%
D0A8X3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	88%
D0N4E2	Phytophthora infestans (strain T30-4)	26%	81%
E9AD33	Leishmania major	25%	99%
E9AEB1	Leishmania major	24%	72%
E9AFZ2	Leishmania major	26%	68%
E9AGS0	Leishmania infantum	24%	79%
E9AHC6	Leishmania infantum	26%	99%
E9AKG1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9ALA7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9ALF2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9ARW9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	79%
E9ASL0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	66%
E9AVR5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	87%
E9AX78	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	92%
E9AXW8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	83%
E9AYV7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	99%
E9B2B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	75%
E9B436	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	72%
E9B4Z4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	84%
E9B745	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	68%
O00506	Homo sapiens	27%	76%
O14047	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
O14305	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	69%
O14408	Metarhizium anisopliae	27%	85%
O14733	Homo sapiens	27%	77%
O14757	Homo sapiens	26%	68%
O23304	Arabidopsis thaliana	26%	66%
O35280	Mus musculus	27%	68%
O35942	Mus musculus	27%	73%
O43293	Homo sapiens	25%	71%
O54784	Mus musculus	25%	72%
O59790	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	91%
O60145	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	81%
O61122	Dictyostelium discoideum	27%	68%
O61267	Drosophila melanogaster	26%	68%
O88764	Rattus norvegicus	25%	72%
O94768	Homo sapiens	26%	87%
P11275	Rattus norvegicus	25%	68%
P11798	Mus musculus	25%	68%
P22517	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	72%
P24923	Medicago sativa	28%	100%
P27466	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	72%
P35567	Xenopus laevis	27%	100%
P51955	Homo sapiens	26%	73%
P9WI74	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	26%	68%
P9WI75	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	26%	68%
Q00771	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	26%	78%
Q07192	Xenopus laevis	25%	72%
Q07250	Malus domestica	26%	78%
Q09170	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	70%
Q0CQK1	Aspergillus terreus (strain NIH 2624 / FGSC A1156)	27%	74%
Q13043	Homo sapiens	26%	66%
Q20085	Caenorhabditis elegans	24%	69%
Q21307	Caenorhabditis elegans	27%	93%
Q24324	Drosophila melanogaster	26%	82%
Q2HJF7	Bos taurus	25%	66%
Q2UC58	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	28%	78%
Q38772	Antirrhinum majus	26%	100%
Q3SWY6	Bos taurus	27%	76%
Q4KSH7	Rattus norvegicus	27%	77%
Q4Q2Z2	Leishmania major	25%	84%
Q4Q3Y9	Leishmania major	22%	72%
Q4Q5T9	Leishmania major	23%	75%
Q4Q9K2	Leishmania major	26%	83%
Q4QA98	Leishmania major	25%	92%
Q4QBR6	Leishmania major	26%	87%
Q4QDK3	Leishmania major	24%	79%
Q4QIJ7	Leishmania major	27%	100%
Q4QJE7	Leishmania major	28%	100%
Q4V3C8	Arabidopsis thaliana	29%	66%
Q4V7Q6	Xenopus laevis	24%	69%
Q4WYR6	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	28%	76%
Q54E34	Dictyostelium discoideum	30%	92%
Q54JG7	Dictyostelium discoideum	25%	67%
Q5E9L6	Bos taurus	26%	66%
Q5HZ38	Arabidopsis thaliana	25%	79%
Q5QN75	Oryza sativa subsp. japonica	23%	91%
Q5RCC4	Pongo abelii	25%	68%
Q5ZJK4	Gallus gallus	26%	66%
Q5ZKI0	Gallus gallus	25%	67%
Q64261	Mus musculus	28%	99%
Q6C3J2	Yarrowia lipolytica (strain CLIB 122 / E 150)	25%	87%
Q6CWQ4	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	24%	89%
Q6P3Q4	Xenopus tropicalis	26%	67%
Q6PA14	Xenopus laevis	26%	67%
Q6Z8C8	Oryza sativa subsp. japonica	25%	70%
Q7TZN1	Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)	26%	68%
Q86AD7	Dictyostelium discoideum	21%	82%
Q8AYC9	Gallus gallus	26%	68%
Q8BG48	Mus musculus	26%	87%
Q8GVC7	Phytophthora infestans	26%	81%
Q8RWC9	Arabidopsis thaliana	27%	73%
Q90321	Cyprinus carpio	27%	81%
Q91XS8	Rattus norvegicus	26%	87%
Q91ZN7	Rattus norvegicus	26%	68%
Q92207	Candida albicans (strain SC5314 / ATCC MYA-2876)	24%	86%
Q93VD3	Arabidopsis thaliana	27%	67%
Q99JT2	Mus musculus	25%	78%
Q99KH8	Mus musculus	24%	75%
Q9DG02	Xenopus laevis	25%	68%
Q9GRT1	Leishmania mexicana	24%	92%
Q9JI11	Mus musculus	26%	66%
Q9LT35	Arabidopsis thaliana	29%	78%
Q9P289	Homo sapiens	25%	78%
Q9UEE5	Homo sapiens	26%	78%
Q9UQM7	Homo sapiens	25%	68%
Q9Y6E0	Homo sapiens	25%	73%
Q9Z2W1	Mus musculus	26%	76%
V5B871	Trypanosoma cruzi	25%	75%
V5B8E0	Trypanosoma cruzi	27%	92%
V5B9R6	Trypanosoma cruzi	26%	92%
V5BC61	Trypanosoma cruzi	27%	100%
V5BCH2	Trypanosoma cruzi	26%	82%
V5BG98	Trypanosoma cruzi	27%	100%
V5BNV8	Trypanosoma cruzi	25%	100%
V5BP88	Trypanosoma cruzi	24%	70%
V5BQF4	Trypanosoma cruzi	24%	87%
V5BSM0	Trypanosoma cruzi	47%	77%
V5BWC2	Trypanosoma cruzi	28%	100%
V5DMJ4	Trypanosoma cruzi	26%	95%
V5DTI8	Trypanosoma cruzi	26%	82%