LeishMANIAdb
  • Home
  • Browse
  • Manual
  • FAQ
  • Download

Putative serine/threonine-protein kinase

Quick info Annotations Function or PPIs Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Putative serine/threonine-protein kinase
Gene product:
Serine/threonine-protein kinase NEK6, putative
Species:
Leishmania major
UniProt:
E9AEC9_LEIMA
TriTrypDb:
LmjF.29.2670 , LMJLV39_290034900 * , LMJSD75_290035200
Length:
521

Annotations

LeishMANIAdb annotations

A large and apprently artificial collection of diverse kinetoplastid protein kinases. None of them appear to be TM.

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 6
Forrest at al. (procyclic) no yes: 6
Silverman et al. no yes: 0
Pissara et al. no yes: 15
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 5
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 20
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 1, no: 55
NetGPI no yes: 0, no: 56
Cellular components
Term Name Level Count
GO:0016020 membrane 2 1
GO:0110165 cellular anatomical entity 1 6
GO:0005737 cytoplasm 2 5
GO:0031974 membrane-enclosed lumen 2 2
GO:0031981 nuclear lumen 5 2
GO:0032838 plasma membrane bounded cell projection cytoplasm 4 2
GO:0043233 organelle lumen 3 2
GO:0070013 intracellular organelle lumen 4 2
GO:0097014 ciliary plasm 5 2
GO:0099568 cytoplasmic region 3 2
GO:0005930 axoneme 2 1
GO:0005952 cAMP-dependent protein kinase complex 3 1
GO:0032991 protein-containing complex 1 1
GO:0061695 transferase complex, transferring phosphorus-containing groups 4 1
GO:0140535 intracellular protein-containing complex 2 1
GO:1902494 catalytic complex 2 1
GO:1902554 serine/threonine protein kinase complex 6 1
GO:1902911 protein kinase complex 5 1
GO:1990234 transferase complex 3 1
GO:0005634 nucleus 5 1
GO:0043226 organelle 2 1
GO:0043227 membrane-bounded organelle 3 1
GO:0043229 intracellular organelle 3 1
GO:0043231 intracellular membrane-bounded organelle 4 1

Expansion

Sequence features

E9AEC9
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEC9

Function

Biological processes
Term Name Level Count
GO:0006468 protein phosphorylation 5 57
GO:0006793 phosphorus metabolic process 3 57
GO:0006796 phosphate-containing compound metabolic process 4 57
GO:0006807 nitrogen compound metabolic process 2 57
GO:0008152 metabolic process 1 57
GO:0009987 cellular process 1 57
GO:0016310 phosphorylation 5 57
GO:0019538 protein metabolic process 3 57
GO:0036211 protein modification process 4 57
GO:0043170 macromolecule metabolic process 3 57
GO:0043412 macromolecule modification 4 57
GO:0044237 cellular metabolic process 2 57
GO:0044238 primary metabolic process 2 57
GO:0071704 organic substance metabolic process 2 57
GO:1901564 organonitrogen compound metabolic process 3 57
GO:0000281 mitotic cytokinesis 4 1
GO:0000910 cytokinesis 3 1
GO:0018105 peptidyl-serine phosphorylation 6 3
GO:0018193 peptidyl-amino acid modification 5 3
GO:0018209 peptidyl-serine modification 6 3
GO:0022402 cell cycle process 2 1
GO:0061640 cytoskeleton-dependent cytokinesis 4 1
GO:1903047 mitotic cell cycle process 3 1
GO:0007165 signal transduction 2 4
GO:0035556 intracellular signal transduction 3 3
GO:0050789 regulation of biological process 2 4
GO:0050794 regulation of cellular process 3 4
GO:0065007 biological regulation 1 4
GO:0009266 response to temperature stimulus 3 1
GO:0009628 response to abiotic stimulus 2 1
GO:0050896 response to stimulus 1 1
Molecular functions
Term Name Level Count
GO:0000166 nucleotide binding 3 57
GO:0003824 catalytic activity 1 57
GO:0004672 protein kinase activity 3 57
GO:0004674 protein serine/threonine kinase activity 4 46
GO:0005488 binding 1 57
GO:0005524 ATP binding 5 57
GO:0016301 kinase activity 4 57
GO:0016740 transferase activity 2 57
GO:0016772 transferase activity, transferring phosphorus-containing groups 3 57
GO:0016773 phosphotransferase activity, alcohol group as acceptor 4 57
GO:0017076 purine nucleotide binding 4 57
GO:0030554 adenyl nucleotide binding 5 57
GO:0032553 ribonucleotide binding 3 57
GO:0032555 purine ribonucleotide binding 4 57
GO:0032559 adenyl ribonucleotide binding 5 57
GO:0035639 purine ribonucleoside triphosphate binding 4 57
GO:0036094 small molecule binding 2 57
GO:0043167 ion binding 2 57
GO:0043168 anion binding 3 57
GO:0097159 organic cyclic compound binding 2 57
GO:0097367 carbohydrate derivative binding 2 57
GO:0140096 catalytic activity, acting on a protein 2 57
GO:1901265 nucleoside phosphate binding 3 57
GO:1901363 heterocyclic compound binding 2 57
GO:0043169 cation binding 3 5
GO:0046872 metal ion binding 4 5
GO:0004707 MAP kinase activity 5 9
GO:0004690 cyclic nucleotide-dependent protein kinase activity 5 1
GO:0004691 cAMP-dependent protein kinase activity 6 1
GO:0004140 dephospho-CoA kinase activity 5 1

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_C14_Caspase3-7 503 507 PF00656 0.386
CLV_NRD_NRD_1 215 217 PF00675 0.224
CLV_NRD_NRD_1 260 262 PF00675 0.304
CLV_NRD_NRD_1 443 445 PF00675 0.473
CLV_PCSK_KEX2_1 215 217 PF00082 0.217
CLV_PCSK_KEX2_1 260 262 PF00082 0.362
CLV_PCSK_KEX2_1 442 444 PF00082 0.448
CLV_PCSK_PC7_1 256 262 PF00082 0.321
CLV_PCSK_SKI1_1 142 146 PF00082 0.325
CLV_PCSK_SKI1_1 251 255 PF00082 0.323
CLV_PCSK_SKI1_1 385 389 PF00082 0.542
CLV_PCSK_SKI1_1 516 520 PF00082 0.599
DEG_SPOP_SBC_1 397 401 PF00917 0.504
DOC_CKS1_1 179 184 PF01111 0.238
DOC_CYCLIN_RxL_1 431 439 PF00134 0.448
DOC_CYCLIN_yCln2_LP_2 214 220 PF00134 0.239
DOC_CYCLIN_yCln2_LP_2 272 278 PF00134 0.487
DOC_MAPK_gen_1 142 151 PF00069 0.330
DOC_MAPK_gen_1 58 65 PF00069 0.263
DOC_PP2B_LxvP_1 272 275 PF13499 0.404
DOC_PP4_FxxP_1 295 298 PF00568 0.515
DOC_PP4_FxxP_1 81 84 PF00568 0.310
DOC_USP7_MATH_1 363 367 PF00917 0.691
DOC_USP7_MATH_1 383 387 PF00917 0.667
DOC_USP7_MATH_1 423 427 PF00917 0.439
DOC_USP7_MATH_1 49 53 PF00917 0.321
DOC_USP7_MATH_1 85 89 PF00917 0.193
DOC_USP7_UBL2_3 154 158 PF12436 0.251
DOC_USP7_UBL2_3 385 389 PF12436 0.544
DOC_WW_Pin1_4 178 183 PF00397 0.300
DOC_WW_Pin1_4 358 363 PF00397 0.630
DOC_WW_Pin1_4 401 406 PF00397 0.557
LIG_14-3-3_CanoR_1 442 448 PF00244 0.431
LIG_14-3-3_CanoR_1 492 500 PF00244 0.572
LIG_APCC_ABBA_1 268 273 PF00400 0.427
LIG_APCC_ABBA_1 335 340 PF00400 0.474
LIG_APCC_ABBAyCdc20_2 444 450 PF00400 0.463
LIG_BRCT_BRCA1_1 111 115 PF00533 0.178
LIG_BRCT_BRCA1_1 146 150 PF00533 0.197
LIG_deltaCOP1_diTrp_1 200 208 PF00928 0.223
LIG_EH1_1 161 169 PF00400 0.321
LIG_FHA_1 115 121 PF00498 0.335
LIG_FHA_1 144 150 PF00498 0.246
LIG_FHA_1 24 30 PF00498 0.250
LIG_FHA_1 310 316 PF00498 0.453
LIG_FHA_1 38 44 PF00498 0.267
LIG_FHA_1 407 413 PF00498 0.667
LIG_FHA_2 261 267 PF00498 0.248
LIG_FHA_2 430 436 PF00498 0.518
LIG_FHA_2 98 104 PF00498 0.199
LIG_LIR_Apic_2 181 187 PF02991 0.328
LIG_LIR_Apic_2 303 309 PF02991 0.516
LIG_LIR_Apic_2 79 84 PF02991 0.310
LIG_LIR_Gen_1 14 21 PF02991 0.367
LIG_LIR_Gen_1 221 231 PF02991 0.353
LIG_LIR_Gen_1 336 345 PF02991 0.416
LIG_LIR_Gen_1 94 101 PF02991 0.347
LIG_LIR_Nem_3 14 18 PF02991 0.380
LIG_LIR_Nem_3 221 227 PF02991 0.355
LIG_LIR_Nem_3 27 33 PF02991 0.448
LIG_LIR_Nem_3 336 341 PF02991 0.420
LIG_LIR_Nem_3 513 518 PF02991 0.454
LIG_LIR_Nem_3 94 98 PF02991 0.355
LIG_NRBOX 423 429 PF00104 0.437
LIG_Pex14_2 77 81 PF04695 0.197
LIG_SH2_CRK 30 34 PF00017 0.306
LIG_SH2_STAP1 302 306 PF00017 0.516
LIG_SH2_STAT5 184 187 PF00017 0.295
LIG_SH2_STAT5 302 305 PF00017 0.533
LIG_SH2_STAT5 41 44 PF00017 0.210
LIG_SH2_STAT5 95 98 PF00017 0.418
LIG_SH3_3 273 279 PF00018 0.447
LIG_SH3_3 310 316 PF00018 0.647
LIG_SH3_3 375 381 PF00018 0.634
LIG_SH3_3 66 72 PF00018 0.204
LIG_SUMO_SIM_anti_2 62 67 PF11976 0.223
LIG_SUMO_SIM_anti_2 88 94 PF11976 0.286
LIG_SUMO_SIM_par_1 456 464 PF11976 0.470
LIG_TRAF2_1 263 266 PF00917 0.225
LIG_TRAF2_1 52 55 PF00917 0.250
LIG_TRAF2_1 59 62 PF00917 0.287
LIG_WRC_WIRS_1 428 433 PF05994 0.419
MOD_CDK_SPxxK_3 401 408 PF00069 0.486
MOD_CK1_1 23 29 PF00069 0.265
MOD_CK1_1 277 283 PF00069 0.409
MOD_CK1_1 376 382 PF00069 0.553
MOD_CK1_1 404 410 PF00069 0.647
MOD_CK1_1 453 459 PF00069 0.729
MOD_CK1_1 471 477 PF00069 0.452
MOD_CK2_1 230 236 PF00069 0.237
MOD_CK2_1 241 247 PF00069 0.197
MOD_CK2_1 260 266 PF00069 0.470
MOD_CK2_1 293 299 PF00069 0.476
MOD_CK2_1 429 435 PF00069 0.689
MOD_CK2_1 466 472 PF00069 0.567
MOD_CK2_1 49 55 PF00069 0.274
MOD_GlcNHglycan 22 25 PF01048 0.267
MOD_GlcNHglycan 295 298 PF01048 0.521
MOD_GlcNHglycan 318 321 PF01048 0.528
MOD_GlcNHglycan 332 335 PF01048 0.485
MOD_GlcNHglycan 365 368 PF01048 0.639
MOD_GlcNHglycan 375 378 PF01048 0.653
MOD_GlcNHglycan 384 388 PF01048 0.702
MOD_GSK3_1 129 136 PF00069 0.210
MOD_GSK3_1 174 181 PF00069 0.319
MOD_GSK3_1 196 203 PF00069 0.236
MOD_GSK3_1 20 27 PF00069 0.384
MOD_GSK3_1 256 263 PF00069 0.308
MOD_GSK3_1 280 287 PF00069 0.452
MOD_GSK3_1 33 40 PF00069 0.260
MOD_GSK3_1 372 379 PF00069 0.619
MOD_GSK3_1 383 390 PF00069 0.537
MOD_GSK3_1 393 400 PF00069 0.639
MOD_GSK3_1 423 430 PF00069 0.452
MOD_N-GLC_1 33 38 PF02516 0.320
MOD_N-GLC_1 372 377 PF02516 0.577
MOD_N-GLC_1 49 54 PF02516 0.273
MOD_NEK2_1 144 149 PF00069 0.321
MOD_NEK2_1 150 155 PF00069 0.316
MOD_NEK2_1 300 305 PF00069 0.584
MOD_NEK2_1 341 346 PF00069 0.496
MOD_NEK2_1 372 377 PF00069 0.505
MOD_NEK2_1 406 411 PF00069 0.498
MOD_NEK2_1 427 432 PF00069 0.523
MOD_NEK2_1 77 82 PF00069 0.269
MOD_PIKK_1 280 286 PF00454 0.424
MOD_PIKK_1 361 367 PF00454 0.497
MOD_PKA_1 260 266 PF00069 0.265
MOD_PKA_1 388 394 PF00069 0.599
MOD_PKA_1 44 50 PF00069 0.298
MOD_PKA_1 442 448 PF00069 0.510
MOD_PKA_2 260 266 PF00069 0.285
MOD_PKA_2 322 328 PF00069 0.508
MOD_PKA_2 442 448 PF00069 0.510
MOD_PKA_2 491 497 PF00069 0.461
MOD_PKA_2 77 83 PF00069 0.201
MOD_PKB_1 107 115 PF00069 0.173
MOD_Plk_1 242 248 PF00069 0.183
MOD_Plk_1 37 43 PF00069 0.212
MOD_Plk_1 450 456 PF00069 0.449
MOD_Plk_1 85 91 PF00069 0.232
MOD_Plk_2-3 97 103 PF00069 0.265
MOD_Plk_4 163 169 PF00069 0.197
MOD_Plk_4 174 180 PF00069 0.288
MOD_Plk_4 423 429 PF00069 0.449
MOD_Plk_4 443 449 PF00069 0.434
MOD_Plk_4 478 484 PF00069 0.414
MOD_Plk_4 77 83 PF00069 0.204
MOD_ProDKin_1 178 184 PF00069 0.300
MOD_ProDKin_1 358 364 PF00069 0.627
MOD_ProDKin_1 401 407 PF00069 0.556
MOD_SUMO_for_1 173 176 PF00179 0.265
MOD_SUMO_for_1 381 384 PF00179 0.538
MOD_SUMO_for_1 59 62 PF00179 0.206
MOD_SUMO_rev_2 446 454 PF00179 0.444
MOD_SUMO_rev_2 513 518 PF00179 0.428
TRG_DiLeu_BaLyEn_6 287 292 PF01217 0.468
TRG_ENDOCYTIC_2 15 18 PF00928 0.280
TRG_ENDOCYTIC_2 30 33 PF00928 0.391
TRG_ENDOCYTIC_2 515 518 PF00928 0.469
TRG_ENDOCYTIC_2 95 98 PF00928 0.389
TRG_ER_diArg_1 214 216 PF00400 0.221
TRG_ER_diArg_1 260 262 PF00400 0.321
TRG_ER_diArg_1 288 291 PF00400 0.513
TRG_ER_diArg_1 442 444 PF00400 0.480
TRG_NES_CRM1_1 221 234 PF08389 0.197
TRG_Pf-PMV_PEXEL_1 138 143 PF00026 0.346
TRG_Pf-PMV_PEXEL_1 290 294 PF00026 0.487
TRG_Pf-PMV_PEXEL_1 434 439 PF00026 0.467
TRG_PTS2 1 21 PF00400 0.366

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N1PEV2 Leptomonas seymouri 65% 96%
A0A0S4JIJ6 Bodo saltans 26% 92%
A0A1X0P963 Trypanosomatidae 46% 100%
A0A3Q8IAQ1 Leishmania donovani 32% 100%
A0A3Q8IC87 Leishmania donovani 29% 100%
A0A3Q8IIG1 Leishmania donovani 33% 100%
A0A3Q8INQ4 Leishmania donovani 26% 100%
A0A3S5H5G0 Leishmania donovani 26% 100%
A0A3S5H789 Leishmania donovani 26% 100%
A0A3S5H7M0 Leishmania donovani 95% 100%
A0A3S7WTN9 Leishmania donovani 31% 100%
A0A3S7WWE7 Leishmania donovani 24% 100%
A0A3S7WYP4 Leishmania donovani 27% 100%
A0A3S7X2W3 Leishmania donovani 28% 100%
A0A3S7X2Z6 Leishmania donovani 27% 100%
A0A3S7X9R4 Leishmania donovani 30% 100%
A4H459 Leishmania braziliensis 26% 100%
A4H8C4 Leishmania braziliensis 32% 100%
A4HBL4 Leishmania braziliensis 23% 100%
A4HCE6 Leishmania braziliensis 33% 100%
A4HED7 Leishmania braziliensis 26% 100%
A4HH03 Leishmania braziliensis 32% 100%
A4HHN1 Leishmania braziliensis 28% 100%
A4HHR5 Leishmania braziliensis 76% 100%
A4HHY4 Leishmania braziliensis 27% 100%
A4HN71 Leishmania braziliensis 31% 100%
A4HSE2 Leishmania infantum 26% 100%
A4HWP5 Leishmania infantum 31% 100%
A4HYX6 Leishmania infantum 26% 100%
A4HZA2 Leishmania infantum 24% 100%
A4HZV1 Leishmania infantum 29% 100%
A4HZW8 Leishmania infantum 32% 100%
A4I140 Leishmania infantum 27% 100%
A4I1T4 Leishmania infantum 26% 100%
A4I435 Leishmania infantum 33% 100%
A4I4U6 Leishmania infantum 28% 100%
A4I4X5 Leishmania infantum 95% 100%
A4I564 Leishmania infantum 27% 100%
A4IBT4 Leishmania infantum 29% 100%
A4IBT9 Leishmania infantum 30% 100%
C9ZKX6 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 44% 100%
E9AKB7 Leishmania mexicana (strain MHOM/GT/2001/U1103) 26% 100%
E9ALF7 Leishmania mexicana (strain MHOM/GT/2001/U1103) 94% 100%
E9ALJ2 Leishmania mexicana (strain MHOM/GT/2001/U1103) 28% 100%
E9AQF3 Leishmania mexicana (strain MHOM/GT/2001/U1103) 31% 100%
E9AUY2 Leishmania mexicana (strain MHOM/GT/2001/U1103) 23% 100%
E9AVR5 Leishmania mexicana (strain MHOM/GT/2001/U1103) 29% 100%
E9AVS7 Leishmania mexicana (strain MHOM/GT/2001/U1103) 32% 100%
E9AX78 Leishmania mexicana (strain MHOM/GT/2001/U1103) 27% 100%
E9AXW8 Leishmania mexicana (strain MHOM/GT/2001/U1103) 26% 100%
E9B0C2 Leishmania mexicana (strain MHOM/GT/2001/U1103) 34% 100%
E9B0G1 Leishmania mexicana (strain MHOM/GT/2001/U1103) 27% 100%
E9B6S4 Leishmania mexicana (strain MHOM/GT/2001/U1103) 30% 100%
E9B6S9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 30% 100%
O80396 Arabidopsis thaliana 26% 100%
Q4Q7S2 Leishmania major 27% 100%
Q4Q7W2 Leishmania major 33% 100%
Q4Q9K2 Leishmania major 26% 100%
Q4QA98 Leishmania major 28% 100%
Q4QBQ2 Leishmania major 32% 100%
Q4QBR6 Leishmania major 29% 100%
Q4QCK0 Leishmania major 24% 100%
Q4QF23 Leishmania major 31% 100%
Q4QJJ0 Leishmania major 25% 100%
Q9GNR4 Leishmania major 29% 100%
Q9Y077 Leishmania major 28% 100%
V5C234 Trypanosoma cruzi 47% 96%

Download

Download
LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2024 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS