Putative serine/threonine-protein kinase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Serine/threonine-protein kinase NEK7, putative

Species:

Leishmania major

UniProt:

E9AEB9_LEIMA

TriTrypDb:

LmjF.29.2570 , LMJLV39_290033800 , LMJSD75_290034200

Length:

501

Annotations

LeishMANIAdb annotations

A large and apprently artificial collection of diverse kinetoplastid protein kinases. A subfamily has 2TM regions, but the majority is cytoplasmic.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AEB9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEB9

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	20
GO:0006793	phosphorus metabolic process	3	20
GO:0006796	phosphate-containing compound metabolic process	4	20
GO:0006807	nitrogen compound metabolic process	2	20
GO:0008152	metabolic process	1	20
GO:0009987	cellular process	1	20
GO:0016310	phosphorylation	5	20
GO:0019538	protein metabolic process	3	20
GO:0036211	protein modification process	4	20
GO:0043170	macromolecule metabolic process	3	20
GO:0043412	macromolecule modification	4	20
GO:0044237	cellular metabolic process	2	20
GO:0044238	primary metabolic process	2	20
GO:0071704	organic substance metabolic process	2	20
GO:1901564	organonitrogen compound metabolic process	3	20

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	20
GO:0003824	catalytic activity	1	20
GO:0004672	protein kinase activity	3	20
GO:0004674	protein serine/threonine kinase activity	4	19
GO:0005488	binding	1	20
GO:0005524	ATP binding	5	20
GO:0016301	kinase activity	4	20
GO:0016740	transferase activity	2	20
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	20
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	20
GO:0017076	purine nucleotide binding	4	20
GO:0030554	adenyl nucleotide binding	5	20
GO:0032553	ribonucleotide binding	3	20
GO:0032555	purine ribonucleotide binding	4	20
GO:0032559	adenyl ribonucleotide binding	5	20
GO:0035639	purine ribonucleoside triphosphate binding	4	20
GO:0036094	small molecule binding	2	20
GO:0043167	ion binding	2	20
GO:0043168	anion binding	3	20
GO:0097159	organic cyclic compound binding	2	20
GO:0097367	carbohydrate derivative binding	2	20
GO:0140096	catalytic activity, acting on a protein	2	20
GO:1901265	nucleoside phosphate binding	3	20
GO:1901363	heterocyclic compound binding	2	20

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	39	43	PF00656	0.329
CLV_C14_Caspase3-7	411	415	PF00656	0.603
CLV_C14_Caspase3-7	97	101	PF00656	0.359
CLV_NRD_NRD_1	61	63	PF00675	0.325
CLV_PCSK_KEX2_1	44	46	PF00082	0.318
CLV_PCSK_KEX2_1	61	63	PF00082	0.328
CLV_PCSK_PC1ET2_1	44	46	PF00082	0.318
CLV_PCSK_SKI1_1	109	113	PF00082	0.344
CLV_PCSK_SKI1_1	147	151	PF00082	0.385
CLV_PCSK_SKI1_1	21	25	PF00082	0.386
CLV_PCSK_SKI1_1	226	230	PF00082	0.355
CLV_PCSK_SKI1_1	233	237	PF00082	0.317
CLV_PCSK_SKI1_1	351	355	PF00082	0.554
CLV_PCSK_SKI1_1	366	370	PF00082	0.665
CLV_PCSK_SKI1_1	432	436	PF00082	0.476
DOC_CYCLIN_yCln2_LP_2	219	225	PF00134	0.388
DOC_CYCLIN_yCln2_LP_2	479	485	PF00134	0.373
DOC_MAPK_gen_1	147	156	PF00069	0.399
DOC_USP7_MATH_1	10	14	PF00917	0.543
DOC_USP7_MATH_1	416	420	PF00917	0.644
DOC_USP7_MATH_1	5	9	PF00917	0.608
DOC_USP7_MATH_1	50	54	PF00917	0.374
DOC_USP7_MATH_2	248	254	PF00917	0.474
DOC_USP7_UBL2_3	109	113	PF12436	0.308
DOC_USP7_UBL2_3	159	163	PF12436	0.408
DOC_USP7_UBL2_3	229	233	PF12436	0.347
DOC_USP7_UBL2_3	24	28	PF12436	0.401
DOC_USP7_UBL2_3	362	366	PF12436	0.783
DOC_USP7_UBL2_3	40	44	PF12436	0.287
DOC_WW_Pin1_4	183	188	PF00397	0.385
DOC_WW_Pin1_4	272	277	PF00397	0.391
DOC_WW_Pin1_4	312	317	PF00397	0.715
DOC_WW_Pin1_4	54	59	PF00397	0.442
LIG_BIR_II_1	1	5	PF00653	0.639
LIG_BRCT_BRCA1_1	12	16	PF00533	0.414
LIG_CtBP_PxDLS_1	248	252	PF00389	0.408
LIG_deltaCOP1_diTrp_1	205	213	PF00928	0.372
LIG_EH1_1	166	174	PF00400	0.297
LIG_FHA_1	149	155	PF00498	0.385
LIG_FHA_1	214	220	PF00498	0.462
LIG_FHA_1	244	250	PF00498	0.408
LIG_FHA_1	394	400	PF00498	0.461
LIG_FHA_1	475	481	PF00498	0.379
LIG_FHA_2	225	231	PF00498	0.408
LIG_FHA_2	367	373	PF00498	0.654
LIG_FHA_2	466	472	PF00498	0.613
LIG_FHA_2	55	61	PF00498	0.346
LIG_Integrin_isoDGR_2	430	432	PF01839	0.535
LIG_LIR_Apic_2	186	192	PF02991	0.385
LIG_LIR_Gen_1	446	456	PF02991	0.350
LIG_LIR_Gen_1	90	99	PF02991	0.347
LIG_LIR_Nem_3	446	452	PF02991	0.360
LIG_MAD2	234	242	PF02301	0.349
LIG_MAD2	69	77	PF02301	0.329
LIG_NRBOX	227	233	PF00104	0.368
LIG_NRBOX	475	481	PF00104	0.509
LIG_SH2_CRK	20	24	PF00017	0.329
LIG_SH2_CRK	240	244	PF00017	0.347
LIG_SH2_NCK_1	240	244	PF00017	0.329
LIG_SH2_SRC	240	243	PF00017	0.329
LIG_SH2_SRC	98	101	PF00017	0.385
LIG_SH2_STAT3	385	388	PF00017	0.505
LIG_SH2_STAT5	138	141	PF00017	0.371
LIG_SH2_STAT5	189	192	PF00017	0.385
LIG_SH2_STAT5	277	280	PF00017	0.488
LIG_SH2_STAT5	385	388	PF00017	0.505
LIG_SH2_STAT5	46	49	PF00017	0.329
LIG_SH3_2	325	330	PF14604	0.749
LIG_SH3_3	113	119	PF00018	0.337
LIG_SH3_3	322	328	PF00018	0.719
LIG_SH3_3	71	77	PF00018	0.329
LIG_TRAF2_1	358	361	PF00917	0.688
LIG_TYR_ITIM	212	217	PF00017	0.372
LIG_UBA3_1	341	349	PF00899	0.611
LIG_WRC_WIRS_1	282	287	PF05994	0.487
MOD_CDC14_SPxK_1	315	318	PF00782	0.592
MOD_CDK_SPK_2	54	59	PF00069	0.442
MOD_CDK_SPxK_1	312	318	PF00069	0.720
MOD_CDK_SPxxK_3	54	61	PF00069	0.442
MOD_CK1_1	465	471	PF00069	0.510
MOD_CK2_1	27	33	PF00069	0.374
MOD_CK2_1	281	287	PF00069	0.585
MOD_CK2_1	295	301	PF00069	0.486
MOD_CK2_1	303	309	PF00069	0.574
MOD_CK2_1	331	337	PF00069	0.628
MOD_CK2_1	366	372	PF00069	0.741
MOD_CK2_1	465	471	PF00069	0.513
MOD_CK2_1	54	60	PF00069	0.329
MOD_Cter_Amidation	42	45	PF01082	0.329
MOD_GlcNHglycan	360	365	PF01048	0.652
MOD_GlcNHglycan	418	421	PF01048	0.754
MOD_GlcNHglycan	460	463	PF01048	0.469
MOD_GlcNHglycan	464	467	PF01048	0.493
MOD_GSK3_1	179	186	PF00069	0.385
MOD_GSK3_1	250	257	PF00069	0.381
MOD_GSK3_1	277	284	PF00069	0.434
MOD_GSK3_1	360	367	PF00069	0.719
MOD_GSK3_1	430	437	PF00069	0.556
MOD_GSK3_1	458	465	PF00069	0.505
MOD_GSK3_1	50	57	PF00069	0.399
MOD_N-GLC_1	201	206	PF02516	0.314
MOD_NEK2_1	155	160	PF00069	0.396
MOD_NEK2_1	167	172	PF00069	0.324
MOD_NEK2_1	173	178	PF00069	0.285
MOD_NEK2_1	194	199	PF00069	0.390
MOD_NEK2_1	213	218	PF00069	0.460
MOD_NEK2_1	254	259	PF00069	0.380
MOD_NEK2_1	36	41	PF00069	0.337
MOD_NEK2_1	458	463	PF00069	0.499
MOD_NEK2_2	102	107	PF00069	0.282
MOD_PKA_1	303	309	PF00069	0.519
MOD_PKA_2	265	271	PF00069	0.388
MOD_Plk_1	102	108	PF00069	0.277
MOD_Plk_1	87	93	PF00069	0.435
MOD_Plk_4	168	174	PF00069	0.324
MOD_Plk_4	254	260	PF00069	0.408
MOD_Plk_4	87	93	PF00069	0.403
MOD_ProDKin_1	183	189	PF00069	0.385
MOD_ProDKin_1	272	278	PF00069	0.391
MOD_ProDKin_1	312	318	PF00069	0.720
MOD_ProDKin_1	54	60	PF00069	0.442
MOD_SUMO_for_1	317	320	PF00179	0.568
MOD_SUMO_rev_2	296	305	PF00179	0.556
TRG_DiLeu_BaEn_1	475	480	PF01217	0.443
TRG_DiLeu_BaEn_1	87	92	PF01217	0.329
TRG_DiLeu_BaEn_4	6	12	PF01217	0.443
TRG_ENDOCYTIC_2	20	23	PF00928	0.322
TRG_ENDOCYTIC_2	214	217	PF00928	0.385
TRG_ENDOCYTIC_2	240	243	PF00928	0.374
TRG_Pf-PMV_PEXEL_1	303	307	PF00026	0.720

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2D3	Leptomonas seymouri	31%	96%
A0A0N1HY86	Leptomonas seymouri	79%	96%
A0A0N1I9A0	Leptomonas seymouri	32%	77%
A0A0S4IKH2	Bodo saltans	67%	100%
A0A0S4JIJ6	Bodo saltans	30%	89%
A0A0S4JNA1	Bodo saltans	36%	100%
A0A1X0NLY7	Trypanosomatidae	27%	74%
A0A1X0NUB2	Trypanosomatidae	31%	86%
A0A1X0P863	Trypanosomatidae	32%	100%
A0A1X0P9C4	Trypanosomatidae	73%	100%
A0A3Q8IAQ1	Leishmania donovani	37%	100%
A0A3Q8IB30	Leishmania donovani	31%	100%
A0A3Q8IB74	Leishmania donovani	27%	100%
A0A3Q8IC87	Leishmania donovani	28%	100%
A0A3Q8IHH8	Leishmania donovani	96%	100%
A0A3Q8IIG1	Leishmania donovani	29%	100%
A0A3Q8INQ4	Leishmania donovani	27%	100%
A0A3R7RIN5	Trypanosoma rangeli	72%	100%
A0A3S5H5G0	Leishmania donovani	25%	100%
A0A3S7WTN9	Leishmania donovani	27%	100%
A0A3S7WWE7	Leishmania donovani	26%	100%
A0A3S7WYP4	Leishmania donovani	29%	100%
A0A3S7X2W3	Leishmania donovani	27%	100%
A0A3S7X5M4	Leishmania donovani	32%	76%
A0A3S7X7Y2	Leishmania donovani	27%	100%
A0A3S7X9S2	Leishmania donovani	31%	100%
A0A422N3U8	Trypanosoma rangeli	27%	69%
A0A422NH41	Trypanosoma rangeli	33%	87%
A0A422NT89	Trypanosoma rangeli	30%	76%
A4H459	Leishmania braziliensis	27%	100%
A4H8C4	Leishmania braziliensis	27%	100%
A4H9L8	Leishmania braziliensis	31%	100%
A4HAS1	Leishmania braziliensis	28%	100%
A4HBL4	Leishmania braziliensis	24%	100%
A4HCE6	Leishmania braziliensis	37%	100%
A4HDU3	Leishmania braziliensis	30%	100%
A4HED7	Leishmania braziliensis	28%	100%
A4HH03	Leishmania braziliensis	30%	100%
A4HHN1	Leishmania braziliensis	27%	100%
A4HHQ5	Leishmania braziliensis	86%	99%
A4HJW2	Leishmania braziliensis	41%	100%
A4HKG9	Leishmania braziliensis	33%	76%
A4HN71	Leishmania braziliensis	30%	100%
A4HNI1	Leishmania braziliensis	34%	100%
A4HSE2	Leishmania infantum	25%	100%
A4HUC8	Leishmania infantum	25%	100%
A4HWP5	Leishmania infantum	27%	100%
A4HWS9	Leishmania infantum	25%	100%
A4HXY2	Leishmania infantum	31%	100%
A4HZA2	Leishmania infantum	26%	100%
A4HZV1	Leishmania infantum	28%	100%
A4HZW8	Leishmania infantum	37%	100%
A4I140	Leishmania infantum	29%	100%
A4I1T4	Leishmania infantum	27%	100%
A4I435	Leishmania infantum	29%	100%
A4I4U6	Leishmania infantum	27%	100%
A4I4X0	Leishmania infantum	97%	100%
A4I7Z6	Leishmania infantum	32%	76%
A4I9Y5	Leishmania infantum	27%	100%
C9ZKE1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	100%
C9ZWK2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
D0AA64	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	87%
E9AET0	Leishmania major	23%	100%
E9AFM1	Leishmania major	31%	100%
E9AKB7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9ALG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
E9ALJ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9AQF3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9ARP5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9ARW9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9ASL0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9AUY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
E9AVR5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9AVS7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9AX78	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9AXW8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9B0C2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9B2B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	100%
E9B2V8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	76%
E9B4Z4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B5Y5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
F4JBP3	Arabidopsis thaliana	29%	70%
O13839	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	69%
P22209	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
P51955	Homo sapiens	36%	100%
P62205	Xenopus tropicalis	27%	84%
P70032	Xenopus laevis	27%	84%
P87248	Colletotrichum lindemuthianum	29%	74%
Q27687	Leishmania major	31%	100%
Q2V452	Arabidopsis thaliana	28%	100%
Q4Q2Z2	Leishmania major	27%	100%
Q4Q598	Leishmania major	33%	76%
Q4Q7W2	Leishmania major	29%	100%
Q4Q9K2	Leishmania major	27%	100%
Q4QA98	Leishmania major	28%	100%
Q4QBQ2	Leishmania major	37%	100%
Q4QBR6	Leishmania major	28%	100%
Q4QCK0	Leishmania major	24%	100%
Q4QF23	Leishmania major	26%	100%
Q4QHJ8	Leishmania major	25%	100%
Q4QJJ0	Leishmania major	25%	100%
Q63531	Rattus norvegicus	27%	68%
Q6P2M8	Homo sapiens	29%	100%
Q6PFQ0	Danio rerio	25%	68%
Q6X4A2	Oryza sativa subsp. japonica	26%	100%
Q75L42	Oryza sativa subsp. japonica	26%	100%
Q75LR7	Oryza sativa subsp. japonica	30%	100%
Q7TNJ7	Rattus norvegicus	29%	100%
Q8RXT4	Arabidopsis thaliana	34%	90%
Q8WNU8	Macaca fascicularis	32%	79%
Q91VB2	Mus musculus	29%	100%
Q9N3Z3	Caenorhabditis elegans	27%	100%
Q9WUT3	Mus musculus	27%	68%
Q9Y077	Leishmania major	26%	100%
V5BCH2	Trypanosoma cruzi	27%	100%
V5BHW8	Trypanosoma cruzi	30%	79%
V5BQE9	Trypanosoma cruzi	28%	68%
V5C224	Trypanosoma cruzi	72%	100%