LeishMANIAdb
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ATP-dependent 6-phosphofructokinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
ATP-dependent 6-phosphofructokinase
Gene product:
ATP-dependent 6-phosphofructokinase, glycosomal
Species:
Leishmania major
UniProt:
E9AEB3_LEIMA
TriTrypDb:
LmjF.29.2510 , LMJLV39_290033200 , LMJSD75_290033600
Length:
486

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 0
Forrest at al. (procyclic) no yes: 0
Silverman et al. no yes: 0
Pissara et al. no yes: 0
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. yes yes: 8
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 16
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 0, no: 11
NetGPI no yes: 0, no: 11
Cellular components
Term Name Level Count
GO:0005737 cytoplasm 2 3
GO:0005777 peroxisome 6 10
GO:0020015 glycosome 7 10
GO:0042579 microbody 5 10
GO:0043226 organelle 2 10
GO:0043227 membrane-bounded organelle 3 10
GO:0043229 intracellular organelle 3 10
GO:0043231 intracellular membrane-bounded organelle 4 10
GO:0110165 cellular anatomical entity 1 11
GO:0016020 membrane 2 1

Expansion

Sequence features

E9AEB3
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEB3

Function

Biological processes
Term Name Level Count
GO:0006002 fructose 6-phosphate metabolic process 4 12
GO:0006793 phosphorus metabolic process 3 12
GO:0006796 phosphate-containing compound metabolic process 4 12
GO:0008152 metabolic process 1 12
GO:0009987 cellular process 1 12
GO:0019637 organophosphate metabolic process 3 12
GO:0044237 cellular metabolic process 2 12
GO:0071704 organic substance metabolic process 2 12
GO:1901135 carbohydrate derivative metabolic process 3 12
Molecular functions
Term Name Level Count
GO:0000166 nucleotide binding 3 11
GO:0003824 catalytic activity 1 12
GO:0003872 6-phosphofructokinase activity 6 12
GO:0005488 binding 1 12
GO:0005524 ATP binding 5 11
GO:0008443 phosphofructokinase activity 5 12
GO:0016301 kinase activity 4 12
GO:0016740 transferase activity 2 12
GO:0016772 transferase activity, transferring phosphorus-containing groups 3 12
GO:0016773 phosphotransferase activity, alcohol group as acceptor 4 12
GO:0017076 purine nucleotide binding 4 11
GO:0019200 carbohydrate kinase activity 5 12
GO:0030554 adenyl nucleotide binding 5 11
GO:0032553 ribonucleotide binding 3 11
GO:0032555 purine ribonucleotide binding 4 11
GO:0032559 adenyl ribonucleotide binding 5 11
GO:0035639 purine ribonucleoside triphosphate binding 4 11
GO:0036094 small molecule binding 2 11
GO:0043167 ion binding 2 12
GO:0043168 anion binding 3 11
GO:0043169 cation binding 3 12
GO:0046872 metal ion binding 4 12
GO:0097159 organic cyclic compound binding 2 11
GO:0097367 carbohydrate derivative binding 2 11
GO:1901265 nucleoside phosphate binding 3 11
GO:1901363 heterocyclic compound binding 2 11

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_C14_Caspase3-7 386 390 PF00656 0.497
CLV_C14_Caspase3-7 65 69 PF00656 0.651
CLV_NRD_NRD_1 212 214 PF00675 0.286
CLV_NRD_NRD_1 309 311 PF00675 0.286
CLV_NRD_NRD_1 432 434 PF00675 0.371
CLV_NRD_NRD_1 46 48 PF00675 0.463
CLV_NRD_NRD_1 465 467 PF00675 0.508
CLV_NRD_NRD_1 85 87 PF00675 0.479
CLV_PCSK_FUR_1 126 130 PF00082 0.346
CLV_PCSK_KEX2_1 128 130 PF00082 0.286
CLV_PCSK_KEX2_1 211 213 PF00082 0.286
CLV_PCSK_KEX2_1 309 311 PF00082 0.286
CLV_PCSK_KEX2_1 432 434 PF00082 0.386
CLV_PCSK_KEX2_1 465 467 PF00082 0.486
CLV_PCSK_PC1ET2_1 128 130 PF00082 0.286
CLV_PCSK_PC1ET2_1 211 213 PF00082 0.297
CLV_PCSK_PC7_1 305 311 PF00082 0.372
CLV_PCSK_SKI1_1 115 119 PF00082 0.284
CLV_PCSK_SKI1_1 201 205 PF00082 0.288
CLV_PCSK_SKI1_1 426 430 PF00082 0.382
CLV_PCSK_SKI1_1 43 47 PF00082 0.700
CLV_PCSK_SKI1_1 438 442 PF00082 0.362
CLV_PCSK_SKI1_1 446 450 PF00082 0.429
DEG_APCC_DBOX_1 464 472 PF00400 0.492
DEG_Nend_UBRbox_2 1 3 PF02207 0.740
DOC_CYCLIN_RxL_1 84 95 PF00134 0.528
DOC_MAPK_gen_1 126 133 PF00069 0.546
DOC_MAPK_gen_1 185 192 PF00069 0.572
DOC_MAPK_gen_1 211 219 PF00069 0.486
DOC_MAPK_gen_1 342 349 PF00069 0.497
DOC_MAPK_gen_1 443 452 PF00069 0.428
DOC_MAPK_gen_1 465 473 PF00069 0.510
DOC_MAPK_HePTP_8 413 425 PF00069 0.370
DOC_MAPK_MEF2A_6 185 192 PF00069 0.486
DOC_MAPK_MEF2A_6 342 349 PF00069 0.486
DOC_MAPK_MEF2A_6 416 425 PF00069 0.375
DOC_MAPK_MEF2A_6 443 452 PF00069 0.428
DOC_PP1_RVXF_1 127 134 PF00149 0.486
DOC_PP1_RVXF_1 85 92 PF00149 0.417
DOC_PP4_FxxP_1 220 223 PF00568 0.497
DOC_USP7_MATH_1 427 431 PF00917 0.439
DOC_USP7_UBL2_3 142 146 PF12436 0.565
DOC_WW_Pin1_4 172 177 PF00397 0.424
LIG_14-3-3_CanoR_1 115 120 PF00244 0.486
LIG_14-3-3_CanoR_1 432 438 PF00244 0.477
LIG_APCC_ABBA_1 204 209 PF00400 0.572
LIG_BIR_III_4 337 341 PF00653 0.489
LIG_DLG_GKlike_1 433 441 PF00625 0.505
LIG_eIF4E_1 417 423 PF01652 0.438
LIG_FHA_1 118 124 PF00498 0.570
LIG_FHA_1 396 402 PF00498 0.505
LIG_FHA_1 407 413 PF00498 0.314
LIG_FHA_1 447 453 PF00498 0.443
LIG_FHA_1 7 13 PF00498 0.515
LIG_FHA_2 145 151 PF00498 0.486
LIG_FHA_2 203 209 PF00498 0.534
LIG_FHA_2 21 27 PF00498 0.454
LIG_FHA_2 239 245 PF00498 0.486
LIG_LIR_Apic_2 14 18 PF02991 0.616
LIG_LIR_Apic_2 370 376 PF02991 0.486
LIG_LIR_Nem_3 414 420 PF02991 0.461
LIG_PDZ_Class_1 481 486 PF00595 0.505
LIG_Pex14_2 325 329 PF04695 0.486
LIG_SH2_CRK 15 19 PF00017 0.617
LIG_SH2_CRK 37 41 PF00017 0.517
LIG_SH2_GRB2like 37 40 PF00017 0.466
LIG_SH2_PTP2 373 376 PF00017 0.486
LIG_SH2_PTP2 420 423 PF00017 0.372
LIG_SH2_SRC 207 210 PF00017 0.572
LIG_SH2_SRC 254 257 PF00017 0.497
LIG_SH2_SRC 373 376 PF00017 0.486
LIG_SH2_STAP1 254 258 PF00017 0.497
LIG_SH2_STAT5 373 376 PF00017 0.486
LIG_SH2_STAT5 420 423 PF00017 0.372
LIG_SH3_3 170 176 PF00018 0.484
LIG_SH3_3 291 297 PF00018 0.486
LIG_SUMO_SIM_par_1 317 323 PF11976 0.488
LIG_SUMO_SIM_par_1 397 403 PF11976 0.526
LIG_TRFH_1 420 424 PF08558 0.374
LIG_UBA3_1 421 426 PF00899 0.380
LIG_UBA3_1 478 485 PF00899 0.500
MOD_CDK_SPK_2 172 177 PF00069 0.251
MOD_CK1_1 20 26 PF00069 0.510
MOD_CK1_1 233 239 PF00069 0.338
MOD_CK1_1 94 100 PF00069 0.510
MOD_CK2_1 144 150 PF00069 0.338
MOD_CK2_1 172 178 PF00069 0.388
MOD_CK2_1 202 208 PF00069 0.405
MOD_CK2_1 238 244 PF00069 0.373
MOD_GlcNHglycan 273 276 PF01048 0.349
MOD_GlcNHglycan 284 287 PF01048 0.338
MOD_GlcNHglycan 337 341 PF01048 0.337
MOD_GSK3_1 113 120 PF00069 0.316
MOD_GSK3_1 138 145 PF00069 0.367
MOD_GSK3_1 162 169 PF00069 0.370
MOD_GSK3_1 278 285 PF00069 0.423
MOD_GSK3_1 391 398 PF00069 0.458
MOD_GSK3_1 446 453 PF00069 0.535
MOD_N-GLC_2 289 291 PF02516 0.354
MOD_NEK2_1 162 167 PF00069 0.382
MOD_NEK2_1 179 184 PF00069 0.257
MOD_NEK2_1 230 235 PF00069 0.338
MOD_NEK2_1 311 316 PF00069 0.338
MOD_NEK2_1 391 396 PF00069 0.341
MOD_NEK2_2 202 207 PF00069 0.446
MOD_NEK2_2 367 372 PF00069 0.338
MOD_NEK2_2 411 416 PF00069 0.419
MOD_NEK2_2 427 432 PF00069 0.412
MOD_PIKK_1 196 202 PF00454 0.338
MOD_PKA_2 271 277 PF00069 0.338
MOD_Plk_1 25 31 PF00069 0.568
MOD_Plk_1 446 452 PF00069 0.435
MOD_Plk_1 94 100 PF00069 0.446
MOD_Plk_4 162 168 PF00069 0.338
MOD_Plk_4 179 185 PF00069 0.338
MOD_Plk_4 202 208 PF00069 0.446
MOD_Plk_4 299 305 PF00069 0.399
MOD_Plk_4 374 380 PF00069 0.354
MOD_ProDKin_1 172 178 PF00069 0.251
MOD_SUMO_for_1 351 354 PF00179 0.458
TRG_DiLeu_BaLyEn_6 302 307 PF01217 0.458
TRG_ENDOCYTIC_2 137 140 PF00928 0.338
TRG_ENDOCYTIC_2 420 423 PF00928 0.358
TRG_ER_diArg_1 309 311 PF00400 0.338
TRG_ER_diArg_1 431 433 PF00400 0.396
TRG_ER_diArg_1 464 466 PF00400 0.505
TRG_NES_CRM1_1 462 476 PF08389 0.573
TRG_Pf-PMV_PEXEL_1 309 313 PF00026 0.407

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N1PBL6 Leptomonas seymouri 90% 100%
A0A0S4IQ47 Bodo saltans 60% 98%
A0A1X0P916 Trypanosomatidae 72% 100%
A0A3R7N9H0 Trypanosoma rangeli 72% 100%
A0A3S7X2Y4 Leishmania donovani 99% 100%
A2RE65 Streptococcus pyogenes serotype M5 (strain Manfredo) 25% 100%
A2RKA2 Lactococcus lactis subsp. cremoris (strain MG1363) 26% 100%
A3CM69 Streptococcus sanguinis (strain SK36) 25% 100%
A3N1C8 Actinobacillus pleuropneumoniae serotype 5b (strain L20) 29% 100%
A4HHQ0 Leishmania braziliensis 95% 100%
A4I4W5 Leishmania infantum 99% 100%
A8AXW1 Streptococcus gordonii (strain Challis / ATCC 35105 / BCRC 15272 / CH1 / DL1 / V288) 25% 100%
A9WCU2 Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) 35% 100%
B0BQ64 Actinobacillus pleuropneumoniae serotype 3 (strain JL03) 29% 100%
B1IB66 Streptococcus pneumoniae (strain Hungary19A-6) 25% 100%
B2IP53 Streptococcus pneumoniae (strain CGSP14) 25% 100%
B2V7W7 Sulfurihydrogenibium sp. (strain YO3AOP1) 29% 100%
B3GY11 Actinobacillus pleuropneumoniae serotype 7 (strain AP76) 29% 100%
B4U2M2 Streptococcus equi subsp. zooepidemicus (strain MGCS10565) 25% 100%
B5E3Z8 Streptococcus pneumoniae serotype 19F (strain G54) 25% 100%
B5XLV6 Streptococcus pyogenes serotype M49 (strain NZ131) 25% 100%
B8D1K5 Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) 31% 100%
B8GAA4 Chloroflexus aggregans (strain MD-66 / DSM 9485) 34% 100%
B8ZNU0 Streptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1) 25% 100%
B9LHK4 Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) 35% 100%
C0M8V6 Streptococcus equi subsp. equi (strain 4047) 25% 100%
C0MGJ9 Streptococcus equi subsp. zooepidemicus (strain H70) 25% 100%
C0QQX4 Persephonella marina (strain DSM 14350 / EX-H1) 30% 100%
C1C6M8 Streptococcus pneumoniae (strain 70585) 25% 100%
C1CDP4 Streptococcus pneumoniae (strain JJA) 25% 100%
C1CJY4 Streptococcus pneumoniae (strain P1031) 25% 100%
C1CRZ7 Streptococcus pneumoniae (strain Taiwan19F-14) 25% 100%
C9ZKZ6 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 71% 100%
D7GCU5 Propionibacterium freudenreichii subsp. shermanii (strain ATCC 9614 / DSM 4902 / CIP 103027 / NCIMB 8099 / CIRM-BIA1) 27% 100%
E0RTD9 Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1) 26% 100%
E9ALH3 Leishmania mexicana (strain MHOM/GT/2001/U1103) 97% 100%
G4RK16 Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1) 29% 100%
G4STG9 Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z) 25% 100%
O08333 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) 33% 100%
O15648 Trypanosoma brucei brucei 71% 100%
O33106 Mycobacterium leprae (strain TN) 33% 100%
O51669 Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31) 42% 100%
O83146 Treponema pallidum (strain Nichols) 41% 100%
P0DD02 Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315) 25% 100%
P0DD03 Streptococcus pyogenes serotype M3 (strain SSI-1) 25% 100%
P0DOB5 Lactococcus lactis subsp. lactis (strain IL1403) 26% 100%
P0DOB6 Lactococcus lactis subsp. lactis 26% 100%
P0DOB7 Lactococcus lactis subsp. lactis 26% 100%
P20275 Spiroplasma citri 29% 100%
P29495 Propionibacterium freudenreichii subsp. shermanii (strain ATCC 9614 / DSM 4902 / CIP 103027 / NCIMB 8099 / CIRM-BIA1) 24% 100%
P65691 Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) 34% 100%
P65696 Streptococcus agalactiae serotype III (strain NEM316) 24% 100%
P65697 Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R) 24% 100%
P72830 Synechocystis sp. (strain PCC 6803 / Kazusa) 29% 100%
P9WID6 Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) 34% 100%
P9WID7 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) 34% 100%
Q02YK5 Lactococcus lactis subsp. cremoris (strain SK11) 26% 100%
Q03KB7 Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) 26% 100%
Q04L24 Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) 25% 100%
Q11RE2 Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465) 26% 100%
Q1J694 Streptococcus pyogenes serotype M4 (strain MGAS10750) 25% 100%
Q1JBF7 Streptococcus pyogenes serotype M12 (strain MGAS2096) 25% 100%
Q1JGH6 Streptococcus pyogenes serotype M2 (strain MGAS10270) 25% 100%
Q1JLD9 Streptococcus pyogenes serotype M12 (strain MGAS9429) 25% 100%
Q27651 Entamoeba histolytica 44% 100%
Q27705 Naegleria fowleri 30% 100%
Q3K1E6 Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700) 24% 100%
Q3KSV5 Methylomonas methanica 26% 100%
Q48T86 Streptococcus pyogenes serotype M28 (strain MGAS6180) 25% 100%
Q4E657 Trypanosoma cruzi (strain CL Brener) 72% 100%
Q59126 Amycolatopsis methanolica 34% 100%
Q5XBV2 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) 25% 100%
Q8A624 Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) 28% 100%
Q8A8R5 Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) 29% 100%
Q8DJB1 Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) 30% 100%
Q8DQ85 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) 25% 100%
Q8NR14 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) 31% 100%
Q8P0S6 Streptococcus pyogenes serotype M18 (strain MGAS8232) 25% 100%
Q8RQN5 Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) 30% 100%
Q8VU09 Amycolatopsis methanolica 41% 100%
Q8VYN6 Arabidopsis thaliana 44% 91%
Q8WPP2 Trypanoplasma borreli 57% 99%
Q8XL57 Clostridium perfringens (strain 13 / Type A) 29% 100%
Q8YKG3 Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) 27% 100%
Q94AA4 Arabidopsis thaliana 43% 99%
Q97RC6 Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) 25% 100%
Q98PW8 Mycoplasmopsis pulmonis (strain UAB CTIP) 24% 100%
Q99ZD0 Streptococcus pyogenes serotype M1 25% 100%
Q9AGC0 Amycolatopsis mediterranei (strain S699) 33% 100%
Q9BIC6 Leishmania donovani 99% 100%
Q9C5J7 Arabidopsis thaliana 43% 100%
Q9FC99 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) 31% 100%
Q9FIK0 Arabidopsis thaliana 38% 100%
Q9FKG3 Arabidopsis thaliana 43% 92%
Q9KH71 Dictyoglomus thermophilum 31% 100%
Q9L1L8 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) 33% 100%
Q9L9E3 Streptococcus thermophilus 26% 100%
Q9M076 Arabidopsis thaliana 43% 100%
Q9M0F9 Arabidopsis thaliana 42% 100%
Q9NGP6 Mastigamoeba balamuthi 24% 100%
Q9RWN1 Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) 28% 100%
Q9WYC5 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) 29% 100%
V5DTU9 Trypanosoma cruzi 72% 87%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2025 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS