LeishMANIAdb
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Vesicle-fusing ATPase

Quick info Annotations Function or PPIs Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Vesicle-fusing ATPase
Gene product:
vacuolar protein sorting-associated protein 4
Species:
Leishmania major
UniProt:
E9AEB2_LEIMA
TriTrypDb:
LmjF.29.2500 , LMJLV39_290033100 , LMJSD75_290033500
Length:
445

Annotations

LeishMANIAdb annotations

A large and likely artifical grouping of protease domain carrying proteins related to proteasomal proteases. Only a tiny subgroup (the AFG3-related mitochondrail proteins) seem to have a TM segment.. Localization: Cytoplasmic (by homology) / Mitochondrial (by homology)

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 4
Forrest at al. (procyclic) no yes: 4
Silverman et al. no yes: 0
Pissara et al. no yes: 12
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. yes yes: 5
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 0
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 0, no: 11
NetGPI no yes: 0, no: 11
Cellular components
Term Name Level Count
GO:0000815 ESCRT III complex 3 2
GO:0005737 cytoplasm 2 2
GO:0032838 plasma membrane bounded cell projection cytoplasm 4 2
GO:0032991 protein-containing complex 1 2
GO:0036452 ESCRT complex 2 2
GO:0097014 ciliary plasm 5 2
GO:0098796 membrane protein complex 2 2
GO:0099568 cytoplasmic region 3 2
GO:0110165 cellular anatomical entity 1 12
GO:0016020 membrane 2 10

Expansion

Sequence features

E9AEB2
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AEB2

Function

Biological processes
Term Name Level Count
GO:0006810 transport 3 2
GO:0006996 organelle organization 4 2
GO:0007033 vacuole organization 5 2
GO:0009894 regulation of catabolic process 4 2
GO:0009987 cellular process 1 2
GO:0010506 regulation of autophagy 6 2
GO:0016043 cellular component organization 3 2
GO:0016192 vesicle-mediated transport 4 2
GO:0016197 endosomal transport 4 2
GO:0019222 regulation of metabolic process 3 2
GO:0031323 regulation of cellular metabolic process 4 2
GO:0031329 regulation of cellular catabolic process 5 2
GO:0046907 intracellular transport 3 2
GO:0050789 regulation of biological process 2 2
GO:0050794 regulation of cellular process 3 2
GO:0051179 localization 1 2
GO:0051234 establishment of localization 2 2
GO:0051641 cellular localization 2 2
GO:0051649 establishment of localization in cell 3 2
GO:0065007 biological regulation 1 2
GO:0071840 cellular component organization or biogenesis 2 2
Molecular functions
Term Name Level Count
GO:0000166 nucleotide binding 3 12
GO:0003824 catalytic activity 1 12
GO:0005488 binding 1 12
GO:0005524 ATP binding 5 12
GO:0016462 pyrophosphatase activity 5 12
GO:0016787 hydrolase activity 2 12
GO:0016817 hydrolase activity, acting on acid anhydrides 3 12
GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides 4 12
GO:0016887 ATP hydrolysis activity 7 12
GO:0017076 purine nucleotide binding 4 12
GO:0017111 ribonucleoside triphosphate phosphatase activity 6 12
GO:0030554 adenyl nucleotide binding 5 12
GO:0032553 ribonucleotide binding 3 12
GO:0032555 purine ribonucleotide binding 4 12
GO:0032559 adenyl ribonucleotide binding 5 12
GO:0035639 purine ribonucleoside triphosphate binding 4 12
GO:0036094 small molecule binding 2 12
GO:0043167 ion binding 2 12
GO:0043168 anion binding 3 12
GO:0097159 organic cyclic compound binding 2 12
GO:0097367 carbohydrate derivative binding 2 12
GO:1901265 nucleoside phosphate binding 3 12
GO:1901363 heterocyclic compound binding 2 12

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_C14_Caspase3-7 185 189 PF00656 0.308
CLV_C14_Caspase3-7 234 238 PF00656 0.274
CLV_C14_Caspase3-7 246 250 PF00656 0.274
CLV_NRD_NRD_1 159 161 PF00675 0.419
CLV_NRD_NRD_1 252 254 PF00675 0.274
CLV_NRD_NRD_1 290 292 PF00675 0.274
CLV_NRD_NRD_1 294 296 PF00675 0.274
CLV_NRD_NRD_1 331 333 PF00675 0.345
CLV_NRD_NRD_1 99 101 PF00675 0.492
CLV_PCSK_KEX2_1 159 161 PF00082 0.563
CLV_PCSK_KEX2_1 252 254 PF00082 0.274
CLV_PCSK_KEX2_1 289 291 PF00082 0.274
CLV_PCSK_KEX2_1 294 296 PF00082 0.274
CLV_PCSK_KEX2_1 331 333 PF00082 0.418
CLV_PCSK_KEX2_1 363 365 PF00082 0.397
CLV_PCSK_KEX2_1 52 54 PF00082 0.333
CLV_PCSK_PC1ET2_1 159 161 PF00082 0.563
CLV_PCSK_PC1ET2_1 363 365 PF00082 0.397
CLV_PCSK_PC1ET2_1 52 54 PF00082 0.333
CLV_PCSK_PC7_1 290 296 PF00082 0.274
CLV_PCSK_SKI1_1 100 104 PF00082 0.572
CLV_PCSK_SKI1_1 139 143 PF00082 0.347
CLV_PCSK_SKI1_1 15 19 PF00082 0.314
CLV_PCSK_SKI1_1 164 168 PF00082 0.274
CLV_PCSK_SKI1_1 212 216 PF00082 0.316
CLV_PCSK_SKI1_1 252 256 PF00082 0.274
CLV_PCSK_SKI1_1 346 350 PF00082 0.385
CLV_PCSK_SKI1_1 375 379 PF00082 0.527
CLV_PCSK_SKI1_1 433 437 PF00082 0.438
CLV_PCSK_SKI1_1 71 75 PF00082 0.601
DOC_CDC14_PxL_1 297 305 PF14671 0.329
DOC_CYCLIN_RxL_1 209 216 PF00134 0.274
DOC_CYCLIN_yCln2_LP_2 404 410 PF00134 0.289
DOC_MAPK_gen_1 159 168 PF00069 0.474
DOC_MAPK_gen_1 289 300 PF00069 0.274
DOC_MAPK_HePTP_8 288 300 PF00069 0.274
DOC_MAPK_MEF2A_6 143 150 PF00069 0.355
DOC_MAPK_MEF2A_6 223 232 PF00069 0.286
DOC_MAPK_MEF2A_6 291 300 PF00069 0.274
DOC_PP1_RVXF_1 431 438 PF00149 0.345
DOC_PP2B_LxvP_1 404 407 PF13499 0.289
DOC_USP7_MATH_1 96 100 PF00917 0.564
DOC_USP7_UBL2_3 139 143 PF12436 0.376
DOC_USP7_UBL2_3 223 227 PF12436 0.308
DOC_USP7_UBL2_3 44 48 PF12436 0.289
DOC_WW_Pin1_4 314 319 PF00397 0.415
DOC_WW_Pin1_4 382 387 PF00397 0.274
LIG_14-3-3_CanoR_1 111 117 PF00244 0.602
LIG_14-3-3_CanoR_1 252 262 PF00244 0.274
LIG_Actin_WH2_2 128 145 PF00022 0.369
LIG_APCC_ABBAyCdc20_2 124 130 PF00400 0.396
LIG_BIR_II_1 1 5 PF00653 0.459
LIG_BIR_III_2 390 394 PF00653 0.418
LIG_BRCT_BRCA1_1 1 5 PF00533 0.459
LIG_BRCT_BRCA1_1 228 232 PF00533 0.368
LIG_FHA_1 254 260 PF00498 0.277
LIG_FHA_1 33 39 PF00498 0.395
LIG_FHA_1 353 359 PF00498 0.476
LIG_FHA_1 372 378 PF00498 0.352
LIG_FHA_2 16 22 PF00498 0.314
LIG_FHA_2 183 189 PF00498 0.274
LIG_FHA_2 318 324 PF00498 0.427
LIG_FHA_2 398 404 PF00498 0.298
LIG_FHA_2 409 415 PF00498 0.343
LIG_FHA_2 49 55 PF00498 0.274
LIG_LIR_Gen_1 188 199 PF02991 0.274
LIG_LIR_Nem_3 188 194 PF02991 0.274
LIG_LIR_Nem_3 26 32 PF02991 0.274
LIG_LIR_Nem_3 323 329 PF02991 0.289
LIG_LIR_Nem_3 360 365 PF02991 0.421
LIG_Pex14_2 152 156 PF04695 0.396
LIG_Pex14_2 437 441 PF04695 0.289
LIG_SH2_CRK 169 173 PF00017 0.274
LIG_SH2_GRB2like 45 48 PF00017 0.308
LIG_SH2_NCK_1 25 29 PF00017 0.308
LIG_SH2_PTP2 297 300 PF00017 0.274
LIG_SH2_STAP1 25 29 PF00017 0.308
LIG_SH2_STAT5 178 181 PF00017 0.308
LIG_SH2_STAT5 297 300 PF00017 0.274
LIG_SH3_1 380 386 PF00018 0.274
LIG_SH3_2 370 375 PF14604 0.464
LIG_SH3_3 143 149 PF00018 0.362
LIG_SH3_3 362 368 PF00018 0.393
LIG_SH3_3 380 386 PF00018 0.274
LIG_SUMO_SIM_anti_2 268 276 PF11976 0.274
LIG_UBA3_1 147 151 PF00899 0.516
LIG_UBA3_1 404 412 PF00899 0.418
MOD_CK1_1 115 121 PF00069 0.482
MOD_CK1_1 193 199 PF00069 0.274
MOD_CK1_1 238 244 PF00069 0.274
MOD_CK1_1 283 289 PF00069 0.368
MOD_CK1_1 422 428 PF00069 0.364
MOD_CK1_1 58 64 PF00069 0.452
MOD_CK1_1 88 94 PF00069 0.649
MOD_CK2_1 15 21 PF00069 0.345
MOD_CK2_1 210 216 PF00069 0.400
MOD_CK2_1 241 247 PF00069 0.274
MOD_CK2_1 336 342 PF00069 0.478
MOD_CK2_1 392 398 PF00069 0.311
MOD_CK2_1 48 54 PF00069 0.333
MOD_Cter_Amidation 157 160 PF01082 0.559
MOD_Cter_Amidation 329 332 PF01082 0.345
MOD_GlcNHglycan 117 120 PF01048 0.586
MOD_GlcNHglycan 240 243 PF01048 0.368
MOD_GlcNHglycan 285 288 PF01048 0.274
MOD_GlcNHglycan 336 339 PF01048 0.290
MOD_GlcNHglycan 394 397 PF01048 0.363
MOD_GlcNHglycan 87 90 PF01048 0.653
MOD_GlcNHglycan 96 99 PF01048 0.595
MOD_GSK3_1 111 118 PF00069 0.691
MOD_GSK3_1 190 197 PF00069 0.418
MOD_GSK3_1 330 337 PF00069 0.274
MOD_GSK3_1 418 425 PF00069 0.280
MOD_GSK3_1 88 95 PF00069 0.637
MOD_NEK2_1 112 117 PF00069 0.496
MOD_NEK2_1 142 147 PF00069 0.497
MOD_NEK2_1 192 197 PF00069 0.274
MOD_NEK2_1 210 215 PF00069 0.274
MOD_NEK2_1 275 280 PF00069 0.274
MOD_NEK2_1 419 424 PF00069 0.395
MOD_PKA_2 112 118 PF00069 0.602
MOD_PKA_2 330 336 PF00069 0.399
MOD_PKA_2 419 425 PF00069 0.292
MOD_Plk_4 142 148 PF00069 0.501
MOD_Plk_4 174 180 PF00069 0.274
MOD_Plk_4 187 193 PF00069 0.274
MOD_Plk_4 226 232 PF00069 0.384
MOD_Plk_4 235 241 PF00069 0.368
MOD_Plk_4 317 323 PF00069 0.509
MOD_ProDKin_1 314 320 PF00069 0.417
MOD_ProDKin_1 382 388 PF00069 0.274
MOD_SUMO_for_1 254 257 PF00179 0.274
MOD_SUMO_for_1 43 46 PF00179 0.274
TRG_DiLeu_BaEn_1 235 240 PF01217 0.418
TRG_DiLeu_BaEn_1 401 406 PF01217 0.308
TRG_DiLeu_BaLyEn_6 377 382 PF01217 0.424
TRG_ENDOCYTIC_2 297 300 PF00928 0.274
TRG_ER_diArg_1 252 254 PF00400 0.275
TRG_ER_diArg_1 288 291 PF00400 0.274
TRG_ER_diArg_1 294 296 PF00400 0.274
TRG_NES_CRM1_1 302 316 PF08389 0.408
TRG_Pf-PMV_PEXEL_1 202 206 PF00026 0.274
TRG_Pf-PMV_PEXEL_1 212 216 PF00026 0.274

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N0P897 Leptomonas seymouri 43% 77%
A0A0N1HRH8 Leptomonas seymouri 44% 82%
A0A0N1P9H6 Leptomonas seymouri 87% 100%
A0A0S4IM94 Bodo saltans 44% 96%
A0A0S4IPV5 Bodo saltans 73% 100%
A0A0S4IUM1 Bodo saltans 41% 75%
A0A0S4JAU3 Bodo saltans 33% 67%
A0A1X0NYV7 Trypanosomatidae 43% 79%
A0A1X0P8T7 Trypanosomatidae 33% 100%
A0A1X0P983 Trypanosomatidae 75% 100%
A0A1X0P9Z1 Trypanosomatidae 42% 94%
A0A3Q8IHU1 Leishmania donovani 34% 100%
A0A3R7KPY1 Trypanosoma rangeli 73% 98%
A0A3S7X163 Leishmania donovani 42% 84%
A0A3S7X2R4 Leishmania donovani 99% 100%
A0A422MWE3 Trypanosoma rangeli 32% 69%
A0A422NDS9 Trypanosoma rangeli 32% 100%
A0A422NNS9 Trypanosoma rangeli 43% 100%
A0A422P1K0 Trypanosoma rangeli 43% 80%
A2VDN5 Bos taurus 39% 72%
A4HG81 Leishmania braziliensis 40% 85%
A4HHP9 Leishmania braziliensis 94% 100%
A4I3C0 Leishmania infantum 42% 74%
A4I4W4 Leishmania infantum 99% 100%
A4IB20 Leishmania infantum 34% 100%
A4IHT0 Xenopus tropicalis 38% 68%
A8QFF6 Brugia malayi 40% 98%
A8XV40 Caenorhabditis briggsae 38% 82%
A9RA82 Papio anubis 44% 91%
B0K5A3 Thermoanaerobacter sp. (strain X514) 33% 73%
B2RYN7 Rattus norvegicus 42% 77%
B3EX35 Sorex araneus 44% 91%
B4USW8 Otolemur garnettii 44% 91%
B5X3X5 Salmo salar 46% 92%
B7NZ88 Oryctolagus cuniculus 44% 91%
C9ZKZ7 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 73% 100%
C9ZLZ3 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 41% 97%
C9ZRM0 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 31% 100%
D0A833 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 43% 80%
D0FH76 Bombyx mori 54% 100%
E9AEU9 Leishmania major 34% 100%
E9ALH4 Leishmania mexicana (strain MHOM/GT/2001/U1103) 98% 100%
E9AZK1 Leishmania mexicana (strain MHOM/GT/2001/U1103) 42% 82%
E9B605 Leishmania mexicana (strain MHOM/GT/2001/U1103) 34% 100%
F2Z6D2 Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) 37% 100%
O16299 Caenorhabditis elegans 43% 75%
O43078 Schizosaccharomyces pombe (strain 972 / ATCC 24843) 40% 67%
O61577 Strongylocentrotus purpuratus 42% 86%
O75351 Homo sapiens 54% 100%
O75449 Homo sapiens 45% 91%
P34123 Dictyostelium discoideum 33% 100%
P46467 Mus musculus 54% 100%
P52917 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 55% 100%
P85200 Helianthus annuus 31% 100%
Q05AS3 Xenopus tropicalis 39% 74%
Q09803 Schizosaccharomyces pombe (strain 972 / ATCC 24843) 53% 100%
Q0IIR9 Xenopus tropicalis 44% 90%
Q0VD48 Bos taurus 54% 100%
Q10ZF7 Trichodesmium erythraeum (strain IMS101) 33% 67%
Q1AV13 Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) 35% 68%
Q1HGK7 Gallus gallus 42% 90%
Q4Q8N0 Leishmania major 42% 81%
Q4R407 Macaca fascicularis 45% 91%
Q54KQ7 Dictyostelium discoideum 39% 68%
Q54PT2 Dictyostelium discoideum 52% 100%
Q5AG40 Candida albicans (strain SC5314 / ATCC MYA-2876) 53% 100%
Q5R658 Pongo abelii 54% 100%
Q5RII9 Danio rerio 46% 92%
Q5U3S1 Danio rerio 43% 91%
Q5XIK7 Rattus norvegicus 44% 91%
Q5ZK92 Gallus gallus 41% 73%
Q6AZT2 Xenopus laevis 39% 74%
Q6DDU8 Xenopus laevis 35% 68%
Q6E0V2 Rattus norvegicus 44% 91%
Q6NW58 Danio rerio 40% 78%
Q6PIW4 Homo sapiens 36% 66%
Q719N1 Sus scrofa 40% 72%
Q793F9 Rattus norvegicus 53% 100%
Q8K0T4 Mus musculus 43% 91%
Q8SQK0 Encephalitozoon cuniculi (strain GB-M1) 32% 100%
Q8VEJ9 Mus musculus 53% 100%
Q9BW62 Homo sapiens 44% 91%
Q9PUL2 Xenopus laevis 44% 92%
Q9QYY8 Mus musculus 42% 72%
Q9SEX2 Arabidopsis thaliana 41% 85%
Q9UBP0 Homo sapiens 40% 72%
Q9UN37 Homo sapiens 53% 100%
Q9VQN8 Drosophila melanogaster 38% 85%
Q9WV86 Mus musculus 44% 91%
Q9ZNT0 Arabidopsis thaliana 50% 100%
V5B0U7 Trypanosoma cruzi 43% 80%
V5B5D2 Trypanosoma cruzi 38% 90%
V5BC41 Trypanosoma cruzi 75% 100%
V5BD45 Trypanosoma cruzi 31% 67%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2024 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS