PlsC domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

acyltransferase, putative

Species:

Leishmania major

UniProt:

E9AE89_LEIMA

TriTrypDb:

LmjF.29.2270 , LMJLV39_290030800 , LMJSD75_290031000

Length:

318

Annotations

LeishMANIAdb annotations

This conserved lipid acyltransferase has many re-entrant segments but only one true TM helix. Most closely related to bacterial acyltransferases.. Heavily expanded in kinetoplastids for unknown reasons. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 45

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 34
NetGPI	no	yes: 0, no: 34

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	28
GO:0110165	cellular anatomical entity	1	28

Expansion

Sequence features

E9AE89

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AE89

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	6
GO:0006644	phospholipid metabolic process	4	6
GO:0006650	glycerophospholipid metabolic process	5	6
GO:0006654	phosphatidic acid biosynthetic process	6	6
GO:0006793	phosphorus metabolic process	3	6
GO:0006796	phosphate-containing compound metabolic process	4	6
GO:0008152	metabolic process	1	6
GO:0008610	lipid biosynthetic process	4	6
GO:0008654	phospholipid biosynthetic process	5	6
GO:0009058	biosynthetic process	2	6
GO:0009987	cellular process	1	6
GO:0019637	organophosphate metabolic process	3	6
GO:0044237	cellular metabolic process	2	6
GO:0044238	primary metabolic process	2	6
GO:0044249	cellular biosynthetic process	3	6
GO:0044255	cellular lipid metabolic process	3	6
GO:0045017	glycerolipid biosynthetic process	4	6
GO:0046473	phosphatidic acid metabolic process	6	6
GO:0046474	glycerophospholipid biosynthetic process	5	6
GO:0046486	glycerolipid metabolic process	4	6
GO:0071704	organic substance metabolic process	2	6
GO:0090407	organophosphate biosynthetic process	4	6
GO:1901576	organic substance biosynthetic process	3	6

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	35
GO:0003841	1-acylglycerol-3-phosphate O-acyltransferase activity	7	10
GO:0008374	O-acyltransferase activity	5	10
GO:0016411	acylglycerol O-acyltransferase activity	6	10
GO:0016740	transferase activity	2	35
GO:0016746	acyltransferase activity	3	35
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	10
GO:0042171	lysophosphatidic acid acyltransferase activity	6	10
GO:0071617	lysophospholipid acyltransferase activity	5	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	265	269	PF00656	0.267
CLV_NRD_NRD_1	313	315	PF00675	0.746
CLV_PCSK_FUR_1	311	315	PF00082	0.605
CLV_PCSK_KEX2_1	292	294	PF00082	0.596
CLV_PCSK_KEX2_1	313	315	PF00082	0.784
CLV_PCSK_PC1ET2_1	292	294	PF00082	0.599
CLV_PCSK_SKI1_1	135	139	PF00082	0.501
CLV_PCSK_SKI1_1	218	222	PF00082	0.493
DEG_Nend_Nbox_1	1	3	PF02207	0.235
DOC_CYCLIN_RxL_1	189	199	PF00134	0.220
DOC_MAPK_HePTP_8	28	40	PF00069	0.453
DOC_MAPK_MEF2A_6	139	147	PF00069	0.302
DOC_MAPK_MEF2A_6	31	40	PF00069	0.445
DOC_PP1_RVXF_1	190	197	PF00149	0.333
DOC_PP1_RVXF_1	29	35	PF00149	0.476
DOC_PP1_RVXF_1	48	55	PF00149	0.209
DOC_PP1_RVXF_1	73	80	PF00149	0.315
DOC_PP2B_LxvP_1	143	146	PF13499	0.329
DOC_PP4_FxxP_1	116	119	PF00568	0.298
DOC_USP7_MATH_1	108	112	PF00917	0.302
DOC_WW_Pin1_4	202	207	PF00397	0.267
LIG_14-3-3_CanoR_1	23	28	PF00244	0.532
LIG_14-3-3_CanoR_1	31	37	PF00244	0.475
LIG_14-3-3_CanoR_1	50	55	PF00244	0.260
LIG_Actin_WH2_2	123	141	PF00022	0.365
LIG_AP2alpha_1	116	120	PF02296	0.247
LIG_AP2alpha_2	118	120	PF02296	0.327
LIG_BRCT_BRCA1_1	166	170	PF00533	0.310
LIG_BRCT_BRCA1_1	192	196	PF00533	0.335
LIG_CSL_BTD_1	238	241	PF09270	0.361
LIG_DCNL_PONY_1	1	4	PF03556	0.323
LIG_FHA_1	203	209	PF00498	0.310
LIG_FHA_1	56	62	PF00498	0.334
LIG_GBD_Chelix_1	272	280	PF00786	0.536
LIG_Integrin_isoDGR_2	133	135	PF01839	0.389
LIG_LIR_Apic_2	115	119	PF02991	0.175
LIG_LIR_Gen_1	252	262	PF02991	0.359
LIG_LIR_Gen_1	98	109	PF02991	0.309
LIG_LIR_Nem_3	115	120	PF02991	0.288
LIG_LIR_Nem_3	216	220	PF02991	0.273
LIG_LIR_Nem_3	252	257	PF02991	0.341
LIG_LIR_Nem_3	53	57	PF02991	0.410
LIG_LIR_Nem_3	7	12	PF02991	0.465
LIG_NRBOX	1	7	PF00104	0.273
LIG_NRBOX	24	30	PF00104	0.626
LIG_Pex14_2	116	120	PF04695	0.254
LIG_Pex14_2	158	162	PF04695	0.289
LIG_Pex14_2	79	83	PF04695	0.344
LIG_SH2_PTP2	254	257	PF00017	0.373
LIG_SH2_SRC	254	257	PF00017	0.336
LIG_SH2_STAP1	128	132	PF00017	0.302
LIG_SH2_STAP1	57	61	PF00017	0.366
LIG_SH2_STAT5	161	164	PF00017	0.257
LIG_SH2_STAT5	229	232	PF00017	0.291
LIG_SH2_STAT5	254	257	PF00017	0.315
LIG_SH2_STAT5	262	265	PF00017	0.354
LIG_SH2_STAT5	57	60	PF00017	0.370
LIG_SH2_STAT5	82	85	PF00017	0.336
LIG_SH3_1	244	250	PF00018	0.335
LIG_SH3_3	235	241	PF00018	0.294
LIG_SH3_3	244	250	PF00018	0.310
LIG_SH3_3	5	11	PF00018	0.481
LIG_SH3_3	90	96	PF00018	0.391
LIG_SUMO_SIM_anti_2	26	31	PF11976	0.523
LIG_SUMO_SIM_par_1	1	7	PF11976	0.194
LIG_TYR_ITIM	126	131	PF00017	0.316
LIG_WRC_WIRS_1	214	219	PF05994	0.281
LIG_WRC_WIRS_1	51	56	PF05994	0.318
MOD_CK1_1	216	222	PF00069	0.301
MOD_Cter_Amidation	290	293	PF01082	0.597
MOD_Cter_Amidation	311	314	PF01082	0.793
MOD_GlcNHglycan	164	169	PF01048	0.571
MOD_GlcNHglycan	83	86	PF01048	0.571
MOD_GSK3_1	108	115	PF00069	0.283
MOD_GSK3_1	160	167	PF00069	0.270
MOD_GSK3_1	95	102	PF00069	0.354
MOD_LATS_1	21	27	PF00433	0.431
MOD_N-GLC_1	202	207	PF02516	0.467
MOD_NEK2_1	121	126	PF00069	0.265
MOD_NEK2_1	138	143	PF00069	0.306
MOD_NEK2_1	4	9	PF00069	0.387
MOD_NEK2_1	55	60	PF00069	0.407
MOD_NEK2_2	108	113	PF00069	0.282
MOD_PK_1	23	29	PF00069	0.432
MOD_Plk_4	112	118	PF00069	0.270
MOD_Plk_4	121	127	PF00069	0.276
MOD_Plk_4	138	144	PF00069	0.253
MOD_Plk_4	216	222	PF00069	0.314
MOD_Plk_4	23	29	PF00069	0.567
MOD_Plk_4	4	10	PF00069	0.477
MOD_Plk_4	99	105	PF00069	0.340
MOD_ProDKin_1	202	208	PF00069	0.267
TRG_ENDOCYTIC_2	101	104	PF00928	0.330
TRG_ENDOCYTIC_2	128	131	PF00928	0.338
TRG_ENDOCYTIC_2	13	16	PF00928	0.363
TRG_ENDOCYTIC_2	228	231	PF00928	0.273
TRG_ENDOCYTIC_2	254	257	PF00928	0.330
TRG_ER_diArg_1	311	314	PF00400	0.574
TRG_Pf-PMV_PEXEL_1	282	286	PF00026	0.587
TRG_Pf-PMV_PEXEL_1	314	318	PF00026	0.767

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P455	Leptomonas seymouri	37%	93%
A0A0N0P6B0	Leptomonas seymouri	24%	100%
A0A0N1HTP4	Leptomonas seymouri	31%	100%
A0A0N1I6V1	Leptomonas seymouri	58%	100%
A0A0N1IKX5	Leptomonas seymouri	52%	100%
A0A0S4IQ05	Bodo saltans	38%	94%
A0A0S4IR22	Bodo saltans	34%	100%
A0A0S4JRB2	Bodo saltans	33%	100%
A0A0S4JRP7	Bodo saltans	21%	100%
A0A1X0P9F0	Trypanosomatidae	40%	100%
A0A3Q8IFY7	Leishmania donovani	24%	100%
A0A3R7NKA0	Trypanosoma rangeli	42%	100%
A0A3S5H5E4	Leishmania donovani	31%	74%
A0A3S5H7L8	Leishmania donovani	60%	98%
A0A3S7WNW6	Leishmania donovani	32%	89%
A4H418	Leishmania braziliensis	35%	100%
A4H419	Leishmania braziliensis	32%	94%
A4HHM7	Leishmania braziliensis	50%	100%
A4HKI5	Leishmania braziliensis	23%	100%
A4HSA1	Leishmania infantum	31%	100%
A4HSA2	Leishmania infantum	32%	89%
A4I4U1	Leishmania infantum	92%	98%
A4I4U2	Leishmania infantum	59%	100%
A4I816	Leishmania infantum	24%	100%
C9ZLI8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AE90	Leishmania major	61%	100%
E9AK86	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9ALJ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	53%	100%
E9B2X4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
Q4Q582	Leishmania major	23%	100%
Q9NF90	Leishmania major	33%	89%
Q9NF91	Leishmania major	30%	83%
V5C201	Trypanosoma cruzi	40%	100%