VIT family protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

VIT family protein

Gene product:

VIT family, putative

Species:

Leishmania major

UniProt:

E9AE74_LEIMA

TriTrypDb:

LmjF.29.2120 , LMJLV39_290029200 , LMJSD75_290029300

Length:

297

Annotations

LeishMANIAdb annotations

Part of a conserved family of eukaryotic iron transporters.. Localization: Endosomal (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0005741	mitochondrial outer membrane	5	2
GO:0016020	membrane	2	13
GO:0019867	outer membrane	3	2
GO:0031090	organelle membrane	3	2
GO:0031966	mitochondrial membrane	4	2
GO:0031968	organelle outer membrane	4	2
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0098588	bounding membrane of organelle	4	2
GO:0110165	cellular anatomical entity	1	13
GO:0005737	cytoplasm	2	1
GO:0020022	acidocalcisome	5	1

Expansion

Sequence features

E9AE74

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AE74

Function

Biological processes
Term	Name	Level	Count
GO:0006873	intracellular monoatomic ion homeostasis	4	13
GO:0006875	obsolete intracellular metal ion homeostasis	6	13
GO:0006880	intracellular sequestering of iron ion	4	3
GO:0009987	cellular process	1	13
GO:0019725	cellular homeostasis	2	13
GO:0030003	intracellular monoatomic cation homeostasis	5	13
GO:0030026	intracellular manganese ion homeostasis	8	13
GO:0042592	homeostatic process	3	13
GO:0046916	obsolete intracellular transition metal ion homeostasis	7	13
GO:0048878	chemical homeostasis	4	13
GO:0050801	monoatomic ion homeostasis	5	13
GO:0051179	localization	1	3
GO:0051235	maintenance of location	2	3
GO:0051238	sequestering of metal ion	3	3
GO:0051651	maintenance of location in cell	2	3
GO:0055065	obsolete metal ion homeostasis	7	13
GO:0055071	manganese ion homeostasis	9	13
GO:0055076	obsolete transition metal ion homeostasis	8	13
GO:0055080	monoatomic cation homeostasis	6	13
GO:0055082	intracellular chemical homeostasis	3	13
GO:0065007	biological regulation	1	13
GO:0065008	regulation of biological quality	2	13
GO:0097577	sequestering of iron ion	3	3
GO:0098771	inorganic ion homeostasis	6	13

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	13
GO:0005381	iron ion transmembrane transporter activity	7	3
GO:0005384	manganese ion transmembrane transporter activity	7	13
GO:0008324	monoatomic cation transmembrane transporter activity	4	13
GO:0015075	monoatomic ion transmembrane transporter activity	3	13
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	13
GO:0022857	transmembrane transporter activity	2	13
GO:0022890	inorganic cation transmembrane transporter activity	4	13
GO:0046873	metal ion transmembrane transporter activity	5	13
GO:0046915	transition metal ion transmembrane transporter activity	6	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	116	118	PF00675	0.329
CLV_NRD_NRD_1	37	39	PF00675	0.374
CLV_PCSK_KEX2_1	116	118	PF00082	0.285
CLV_PCSK_SKI1_1	158	162	PF00082	0.261
DEG_Nend_Nbox_1	1	3	PF02207	0.568
DEG_SPOP_SBC_1	177	181	PF00917	0.539
DEG_SPOP_SBC_1	99	103	PF00917	0.415
DOC_CDC14_PxL_1	266	274	PF14671	0.299
DOC_CKS1_1	199	204	PF01111	0.415
DOC_MAPK_gen_1	77	87	PF00069	0.385
DOC_PP2B_LxvP_1	175	178	PF13499	0.446
DOC_USP7_MATH_1	178	182	PF00917	0.512
DOC_USP7_MATH_1	19	23	PF00917	0.541
DOC_USP7_MATH_1	97	101	PF00917	0.443
DOC_WW_Pin1_4	198	203	PF00397	0.405
LIG_14-3-3_CanoR_1	156	161	PF00244	0.461
LIG_BRCT_BRCA1_1	102	106	PF00533	0.369
LIG_Clathr_ClatBox_1	164	168	PF01394	0.463
LIG_FHA_1	199	205	PF00498	0.415
LIG_FHA_1	249	255	PF00498	0.285
LIG_FHA_1	30	36	PF00498	0.595
LIG_FHA_2	141	147	PF00498	0.539
LIG_FHA_2	177	183	PF00498	0.539
LIG_LIR_Gen_1	159	167	PF02991	0.485
LIG_LIR_Gen_1	169	178	PF02991	0.454
LIG_LIR_Nem_3	159	164	PF02991	0.485
LIG_LIR_Nem_3	169	175	PF02991	0.454
LIG_LIR_Nem_3	260	266	PF02991	0.316
LIG_MLH1_MIPbox_1	102	106	PF16413	0.210
LIG_PCNA_PIPBox_1	119	128	PF02747	0.461
LIG_PDZ_Wminus1_1	295	297	PF00595	0.538
LIG_SH2_NCK_1	266	270	PF00017	0.360
LIG_SH2_SRC	266	269	PF00017	0.401
LIG_SH2_STAP1	111	115	PF00017	0.446
LIG_SH2_STAT3	137	140	PF00017	0.446
LIG_SH2_STAT5	105	108	PF00017	0.511
LIG_SH2_STAT5	256	259	PF00017	0.366
LIG_SH2_STAT5	291	294	PF00017	0.381
LIG_SH2_STAT5	98	101	PF00017	0.360
LIG_SH3_3	184	190	PF00018	0.519
LIG_SH3_3	196	202	PF00018	0.218
LIG_SH3_3	64	70	PF00018	0.306
LIG_TRAF2_1	143	146	PF00917	0.461
LIG_TYR_ITIM	170	175	PF00017	0.446
LIG_UBA3_1	133	138	PF00899	0.596
LIG_WRC_WIRS_1	30	35	PF05994	0.672
MOD_CK1_1	100	106	PF00069	0.400
MOD_CK1_1	219	225	PF00069	0.291
MOD_CK1_1	233	239	PF00069	0.404
MOD_CK2_1	140	146	PF00069	0.461
MOD_CK2_1	170	176	PF00069	0.446
MOD_GlcNHglycan	126	129	PF01048	0.339
MOD_GlcNHglycan	221	224	PF01048	0.362
MOD_GlcNHglycan	232	235	PF01048	0.366
MOD_GlcNHglycan	50	53	PF01048	0.258
MOD_GSK3_1	132	139	PF00069	0.513
MOD_GSK3_1	178	185	PF00069	0.504
MOD_GSK3_1	230	237	PF00069	0.383
MOD_GSK3_1	248	255	PF00069	0.274
MOD_NEK2_1	148	153	PF00069	0.459
MOD_NEK2_1	170	175	PF00069	0.435
MOD_NEK2_1	272	277	PF00069	0.422
MOD_NEK2_1	287	292	PF00069	0.381
MOD_NEK2_1	48	53	PF00069	0.303
MOD_NEK2_2	282	287	PF00069	0.306
MOD_PIKK_1	136	142	PF00454	0.521
MOD_Plk_1	19	25	PF00069	0.622
MOD_Plk_4	100	106	PF00069	0.210
MOD_Plk_4	132	138	PF00069	0.564
MOD_Plk_4	156	162	PF00069	0.461
MOD_Plk_4	252	258	PF00069	0.377
MOD_Plk_4	282	288	PF00069	0.412
MOD_ProDKin_1	198	204	PF00069	0.405
MOD_SUMO_for_1	28	31	PF00179	0.669
TRG_DiLeu_BaEn_1	144	149	PF01217	0.477
TRG_DiLeu_BaEn_3	144	150	PF01217	0.502
TRG_DiLeu_BaLyEn_6	199	204	PF01217	0.417
TRG_ENDOCYTIC_2	172	175	PF00928	0.442
TRG_ER_diArg_1	115	117	PF00400	0.555
TRG_ER_diArg_1	210	213	PF00400	0.389
TRG_ER_diArg_1	43	46	PF00400	0.511
TRG_Pf-PMV_PEXEL_1	108	112	PF00026	0.273

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HYN3	Leptomonas seymouri	64%	100%
A0A0S4J089	Bodo saltans	30%	100%
A0A1X0P039	Trypanosomatidae	40%	100%
A0A3Q8IFA4	Leishmania donovani	94%	100%
A0A3R7L265	Trypanosoma rangeli	41%	100%
A4HFD9	Leishmania braziliensis	29%	100%
A4HHL2	Leishmania braziliensis	80%	100%
C9ZLG6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9ACZ8	Leishmania major	29%	100%
E9ALL1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
V5BGJ1	Trypanosoma cruzi	43%	100%