EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AE54_LEIMA

TriTrypDb:

LmjF.29.1930 , LMJLV39_290027000 , LMJSD75_290027200

Length:

604

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	7
GO:0005813	centrosome	3	2
GO:0005815	microtubule organizing center	2	2
GO:0036064	ciliary basal body	3	2
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

E9AE54

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AE54

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	2
GO:0006996	organelle organization	4	2
GO:0007010	cytoskeleton organization	5	2
GO:0007017	microtubule-based process	2	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0019220	regulation of phosphate metabolic process	6	7
GO:0019222	regulation of metabolic process	3	7
GO:0030865	cortical cytoskeleton organization	6	2
GO:0031323	regulation of cellular metabolic process	4	7
GO:0035303	regulation of dephosphorylation	7	7
GO:0050789	regulation of biological process	2	7
GO:0050794	regulation of cellular process	3	7
GO:0051174	regulation of phosphorus metabolic process	5	7
GO:0065007	biological regulation	1	7
GO:0071840	cellular component organization or biogenesis	2	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	7
GO:0005509	calcium ion binding	5	6
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	335	339	PF00656	0.453
CLV_NRD_NRD_1	171	173	PF00675	0.676
CLV_NRD_NRD_1	377	379	PF00675	0.328
CLV_NRD_NRD_1	492	494	PF00675	0.356
CLV_NRD_NRD_1	60	62	PF00675	0.667
CLV_PCSK_KEX2_1	171	173	PF00082	0.675
CLV_PCSK_KEX2_1	241	243	PF00082	0.735
CLV_PCSK_KEX2_1	377	379	PF00082	0.328
CLV_PCSK_KEX2_1	60	62	PF00082	0.494
CLV_PCSK_PC1ET2_1	241	243	PF00082	0.602
CLV_PCSK_SKI1_1	172	176	PF00082	0.543
CLV_PCSK_SKI1_1	365	369	PF00082	0.477
CLV_PCSK_SKI1_1	377	381	PF00082	0.341
CLV_PCSK_SKI1_1	388	392	PF00082	0.387
CLV_PCSK_SKI1_1	431	435	PF00082	0.435
DOC_CKS1_1	525	530	PF01111	0.363
DOC_CYCLIN_RxL_1	374	384	PF00134	0.334
DOC_CYCLIN_yCln2_LP_2	150	153	PF00134	0.471
DOC_CYCLIN_yCln2_LP_2	194	200	PF00134	0.629
DOC_MAPK_gen_1	60	68	PF00069	0.435
DOC_MAPK_MEF2A_6	291	298	PF00069	0.399
DOC_MAPK_MEF2A_6	93	101	PF00069	0.377
DOC_PP1_RVXF_1	375	382	PF00149	0.331
DOC_PP1_RVXF_1	492	499	PF00149	0.335
DOC_PP2B_LxvP_1	150	153	PF13499	0.537
DOC_PP2B_LxvP_1	194	197	PF13499	0.690
DOC_PP2B_LxvP_1	547	550	PF13499	0.393
DOC_USP7_MATH_1	223	227	PF00917	0.623
DOC_USP7_MATH_1	253	257	PF00917	0.578
DOC_USP7_MATH_1	4	8	PF00917	0.587
DOC_USP7_MATH_1	42	46	PF00917	0.474
DOC_USP7_MATH_1	469	473	PF00917	0.401
DOC_USP7_MATH_1	506	510	PF00917	0.379
DOC_USP7_UBL2_3	102	106	PF12436	0.410
DOC_WW_Pin1_4	185	190	PF00397	0.704
DOC_WW_Pin1_4	2	7	PF00397	0.543
DOC_WW_Pin1_4	205	210	PF00397	0.726
DOC_WW_Pin1_4	465	470	PF00397	0.378
DOC_WW_Pin1_4	524	529	PF00397	0.368
DOC_WW_Pin1_4	576	581	PF00397	0.376
LIG_14-3-3_CanoR_1	123	129	PF00244	0.422
LIG_14-3-3_CanoR_1	18	23	PF00244	0.593
LIG_14-3-3_CanoR_1	195	201	PF00244	0.698
LIG_14-3-3_CanoR_1	202	211	PF00244	0.480
LIG_14-3-3_CanoR_1	229	234	PF00244	0.655
LIG_14-3-3_CanoR_1	340	346	PF00244	0.398
LIG_14-3-3_CanoR_1	392	397	PF00244	0.387
LIG_14-3-3_CanoR_1	60	68	PF00244	0.469
LIG_14-3-3_CanoR_1	86	95	PF00244	0.415
LIG_Actin_RPEL_3	571	590	PF02755	0.349
LIG_BIR_II_1	1	5	PF00653	0.656
LIG_BRCT_BRCA1_1	64	68	PF00533	0.515
LIG_CtBP_PxDLS_1	197	201	PF00389	0.605
LIG_deltaCOP1_diTrp_1	39	46	PF00928	0.553
LIG_eIF4E_1	429	435	PF01652	0.438
LIG_FHA_1	14	20	PF00498	0.547
LIG_FHA_1	206	212	PF00498	0.663
LIG_FHA_1	423	429	PF00498	0.353
LIG_FHA_1	438	444	PF00498	0.389
LIG_FHA_1	479	485	PF00498	0.508
LIG_FHA_1	48	54	PF00498	0.494
LIG_FHA_1	521	527	PF00498	0.378
LIG_FHA_1	82	88	PF00498	0.474
LIG_FHA_2	154	160	PF00498	0.578
LIG_FHA_2	34	40	PF00498	0.512
LIG_FHA_2	410	416	PF00498	0.471
LIG_FHA_2	455	461	PF00498	0.460
LIG_FHA_2	484	490	PF00498	0.434
LIG_FHA_2	570	576	PF00498	0.368
LIG_LIR_Gen_1	129	138	PF02991	0.404
LIG_LIR_Gen_1	232	240	PF02991	0.506
LIG_LIR_Gen_1	403	411	PF02991	0.372
LIG_LIR_Gen_1	558	566	PF02991	0.379
LIG_LIR_Nem_3	129	134	PF02991	0.411
LIG_LIR_Nem_3	232	236	PF02991	0.516
LIG_LIR_Nem_3	403	408	PF02991	0.364
LIG_LIR_Nem_3	489	495	PF02991	0.350
LIG_LIR_Nem_3	558	563	PF02991	0.359
LIG_LIR_Nem_3	594	598	PF02991	0.349
LIG_LIR_Nem_3	98	104	PF02991	0.411
LIG_NRBOX	113	119	PF00104	0.400
LIG_NRP_CendR_1	601	604	PF00754	0.471
LIG_PALB2_WD40_1	415	423	PF16756	0.436
LIG_PCNA_yPIPBox_3	270	282	PF02747	0.576
LIG_PTB_Apo_2	277	284	PF02174	0.555
LIG_PTB_Apo_2	529	536	PF02174	0.356
LIG_PTB_Phospho_1	277	283	PF10480	0.556
LIG_REV1ctd_RIR_1	532	540	PF16727	0.353
LIG_SH2_GRB2like	598	601	PF00017	0.357
LIG_SH2_STAP1	131	135	PF00017	0.393
LIG_SH2_STAT3	146	149	PF00017	0.495
LIG_SH2_STAT3	243	246	PF00017	0.544
LIG_SH2_STAT5	283	286	PF00017	0.498
LIG_SH2_STAT5	349	352	PF00017	0.369
LIG_SH2_STAT5	371	374	PF00017	0.317
LIG_SH2_STAT5	478	481	PF00017	0.359
LIG_SH2_STAT5	512	515	PF00017	0.409
LIG_SH3_3	190	196	PF00018	0.622
LIG_SH3_3	284	290	PF00018	0.422
LIG_SH3_3	522	528	PF00018	0.371
LIG_SH3_3	574	580	PF00018	0.376
LIG_SUMO_SIM_par_1	10	16	PF11976	0.541
LIG_SUMO_SIM_par_1	497	503	PF11976	0.326
LIG_TRAF2_1	23	26	PF00917	0.507
LIG_TRAF2_1	37	40	PF00917	0.451
LIG_WRC_WIRS_1	19	24	PF05994	0.531
LIG_WRC_WIRS_1	314	319	PF05994	0.366
LIG_WW_3	288	292	PF00397	0.390
MOD_CDC14_SPxK_1	192	195	PF00782	0.557
MOD_CDK_SPxK_1	189	195	PF00069	0.560
MOD_CK1_1	187	193	PF00069	0.722
MOD_CK1_1	2	8	PF00069	0.605
MOD_CK1_1	205	211	PF00069	0.669
MOD_CK1_1	249	255	PF00069	0.633
MOD_CK1_1	551	557	PF00069	0.352
MOD_CK1_1	594	600	PF00069	0.355
MOD_CK1_1	62	68	PF00069	0.479
MOD_CK2_1	158	164	PF00069	0.639
MOD_CK2_1	33	39	PF00069	0.477
MOD_CK2_1	339	345	PF00069	0.396
MOD_CK2_1	483	489	PF00069	0.440
MOD_CK2_1	569	575	PF00069	0.378
MOD_GlcNHglycan	118	121	PF01048	0.489
MOD_GlcNHglycan	180	184	PF01048	0.770
MOD_GlcNHglycan	204	207	PF01048	0.765
MOD_GlcNHglycan	225	228	PF01048	0.576
MOD_GlcNHglycan	250	254	PF01048	0.690
MOD_GlcNHglycan	398	401	PF01048	0.385
MOD_GSK3_1	185	192	PF00069	0.781
MOD_GSK3_1	225	232	PF00069	0.588
MOD_GSK3_1	249	256	PF00069	0.606
MOD_GSK3_1	392	399	PF00069	0.361
MOD_GSK3_1	409	416	PF00069	0.401
MOD_GSK3_1	465	472	PF00069	0.433
MOD_GSK3_1	520	527	PF00069	0.376
MOD_GSK3_1	565	572	PF00069	0.424
MOD_N-GLC_1	185	190	PF02516	0.638
MOD_NEK2_1	204	209	PF00069	0.634
MOD_NEK2_1	212	217	PF00069	0.564
MOD_NEK2_1	258	263	PF00069	0.544
MOD_NEK2_1	296	301	PF00069	0.406
MOD_NEK2_1	32	37	PF00069	0.494
MOD_NEK2_1	332	337	PF00069	0.406
MOD_NEK2_1	396	401	PF00069	0.356
MOD_NEK2_1	434	439	PF00069	0.457
MOD_NEK2_1	520	525	PF00069	0.406
MOD_NEK2_1	535	540	PF00069	0.399
MOD_NEK2_1	87	92	PF00069	0.443
MOD_NEK2_2	469	474	PF00069	0.398
MOD_PIKK_1	13	19	PF00454	0.536
MOD_PIKK_1	40	46	PF00454	0.530
MOD_PIKK_1	413	419	PF00454	0.411
MOD_PIKK_1	422	428	PF00454	0.339
MOD_PIKK_1	59	65	PF00454	0.377
MOD_PKA_2	122	128	PF00069	0.426
MOD_PKA_2	339	345	PF00069	0.396
MOD_PKA_2	437	443	PF00069	0.383
MOD_PKA_2	535	541	PF00069	0.354
MOD_PKA_2	551	557	PF00069	0.372
MOD_PKA_2	59	65	PF00069	0.481
MOD_Plk_1	121	127	PF00069	0.475
MOD_Plk_1	82	88	PF00069	0.469
MOD_Plk_4	153	159	PF00069	0.575
MOD_Plk_4	254	260	PF00069	0.545
MOD_Plk_4	319	325	PF00069	0.352
MOD_Plk_4	392	398	PF00069	0.363
MOD_Plk_4	454	460	PF00069	0.525
MOD_Plk_4	469	475	PF00069	0.290
MOD_Plk_4	480	486	PF00069	0.375
MOD_Plk_4	551	557	PF00069	0.372
MOD_Plk_4	565	571	PF00069	0.406
MOD_Plk_4	591	597	PF00069	0.338
MOD_Plk_4	64	70	PF00069	0.508
MOD_ProDKin_1	185	191	PF00069	0.704
MOD_ProDKin_1	2	8	PF00069	0.539
MOD_ProDKin_1	205	211	PF00069	0.615
MOD_ProDKin_1	465	471	PF00069	0.370
MOD_ProDKin_1	524	530	PF00069	0.364
MOD_ProDKin_1	576	582	PF00069	0.368
MOD_SUMO_for_1	324	327	PF00179	0.343
TRG_DiLeu_BaEn_1	480	485	PF01217	0.418
TRG_DiLeu_BaEn_1	558	563	PF01217	0.359
TRG_DiLeu_BaEn_1	83	88	PF01217	0.468
TRG_DiLeu_LyEn_5	83	88	PF01217	0.468
TRG_ENDOCYTIC_2	131	134	PF00928	0.398
TRG_ENDOCYTIC_2	314	317	PF00928	0.374
TRG_ENDOCYTIC_2	429	432	PF00928	0.362
TRG_ENDOCYTIC_2	492	495	PF00928	0.347
TRG_ER_diArg_1	377	379	PF00400	0.328
TRG_ER_diArg_1	74	77	PF00400	0.634
TRG_Pf-PMV_PEXEL_1	377	382	PF00026	0.337
TRG_Pf-PMV_PEXEL_1	55	59	PF00026	0.551

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PC66	Leptomonas seymouri	68%	96%
A0A3Q8IFG2	Leishmania donovani	95%	100%
A4HHJ3	Leishmania braziliensis	87%	99%
A4I4Q1	Leishmania infantum	95%	100%
E9ALN1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%