Cytochrome b5 heme-binding domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cytochrome b5-like Heme/Steroid binding domain containing protein, putative

Species:

Leishmania major

UniProt:

E9ADU2_LEIMA

TriTrypDb:

LmjF.29.0868 , LMJLV39_290014700 , LMJSD75_290014700

Length:

168

Annotations

LeishMANIAdb annotations

This cluster contains a high variety of cytochrome b5 homologs. Although all membrane-anchored, some use the N-terminal [type-III] while others the C-terminal TM helix [type-IV]. The heme domain is assumed to be cytoplasmic.. Localization: ER (by homology) / Mitochondrial outer membrane (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0016020	membrane	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ADU2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADU2

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	2
GO:0020037	heme binding	4	2
GO:0046906	tetrapyrrole binding	3	2
GO:0097159	organic cyclic compound binding	2	2
GO:1901363	heterocyclic compound binding	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	156	158	PF00675	0.657
CLV_NRD_NRD_1	29	31	PF00675	0.321
CLV_NRD_NRD_1	59	61	PF00675	0.368
CLV_NRD_NRD_1	79	81	PF00675	0.504
CLV_PCSK_KEX2_1	13	15	PF00082	0.561
CLV_PCSK_KEX2_1	156	158	PF00082	0.657
CLV_PCSK_KEX2_1	29	31	PF00082	0.229
CLV_PCSK_KEX2_1	59	61	PF00082	0.378
CLV_PCSK_KEX2_1	79	81	PF00082	0.498
CLV_PCSK_PC1ET2_1	13	15	PF00082	0.537
DEG_APCC_DBOX_1	28	36	PF00400	0.390
DEG_Nend_Nbox_1	1	3	PF02207	0.687
DOC_CYCLIN_yClb1_LxF_4	2	8	PF00134	0.521
DOC_PP2B_LxvP_1	98	101	PF13499	0.565
DOC_WW_Pin1_4	117	122	PF00397	0.641
DOC_WW_Pin1_4	147	152	PF00397	0.668
DOC_WW_Pin1_4	158	163	PF00397	0.659
DOC_WW_Pin1_4	88	93	PF00397	0.472
LIG_14-3-3_CanoR_1	156	164	PF00244	0.659
LIG_14-3-3_CanoR_1	29	33	PF00244	0.404
LIG_FHA_1	108	114	PF00498	0.658
LIG_FHA_1	136	142	PF00498	0.676
LIG_FHA_1	42	48	PF00498	0.412
LIG_FHA_1	89	95	PF00498	0.475
LIG_FHA_2	103	109	PF00498	0.503
LIG_NRBOX	1	7	PF00104	0.608
LIG_NRP_CendR_1	166	168	PF00754	0.631
LIG_Pex14_2	7	11	PF04695	0.559
LIG_SH2_STAT5	71	74	PF00017	0.499
LIG_TRAF2_1	105	108	PF00917	0.628
LIG_TRAF2_1	132	135	PF00917	0.720
LIG_TRAF2_1	19	22	PF00917	0.356
LIG_UBA3_1	5	13	PF00899	0.589
LIG_WW_3	99	103	PF00397	0.605
MOD_CK1_1	36	42	PF00069	0.431
MOD_CK2_1	102	108	PF00069	0.532
MOD_CK2_1	129	135	PF00069	0.774
MOD_CK2_1	16	22	PF00069	0.351
MOD_GlcNHglycan	131	134	PF01048	0.792
MOD_GlcNHglycan	35	38	PF01048	0.361
MOD_GlcNHglycan	8	11	PF01048	0.543
MOD_GSK3_1	129	136	PF00069	0.807
MOD_GSK3_1	140	147	PF00069	0.602
MOD_NEK2_1	25	30	PF00069	0.222
MOD_NEK2_1	6	11	PF00069	0.560
MOD_NEK2_2	69	74	PF00069	0.496
MOD_PKA_2	155	161	PF00069	0.671
MOD_PKA_2	28	34	PF00069	0.386
MOD_Plk_1	69	75	PF00069	0.496
MOD_Plk_4	36	42	PF00069	0.404
MOD_ProDKin_1	117	123	PF00069	0.642
MOD_ProDKin_1	147	153	PF00069	0.670
MOD_ProDKin_1	158	164	PF00069	0.654
MOD_ProDKin_1	88	94	PF00069	0.469
TRG_ER_diArg_1	155	157	PF00400	0.691
TRG_ER_diArg_1	58	60	PF00400	0.390
TRG_NLS_MonoExtC_3	11	16	PF00514	0.552

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMH0	Leptomonas seymouri	64%	98%
A0A3S7X2B2	Leishmania donovani	96%	100%
A0A3S7X2E8	Leishmania donovani	43%	97%
A4HH96	Leishmania braziliensis	84%	100%
A4I4E2	Leishmania infantum	96%	100%
E9ALY8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%