ADF/Cofilin

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

ADF/Cofilin

Gene product:

ADF/Cofilin

Species:

Leishmania major

UniProt:

E9ADQ2_LEIMA

TriTrypDb:

LmjF.29.0510 , LMJLV39_290010400 , LMJSD75_290010400

Length:

139

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 1
Forrest at al. (procyclic)	no	yes: 1
Silverman et al.	yes	yes: 1
Pissara et al.	yes	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 4

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005856	cytoskeleton	5	11
GO:0015629	actin cytoskeleton	6	11
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0043226	organelle	2	11
GO:0043228	non-membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043232	intracellular non-membrane-bounded organelle	4	11
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

E9ADQ2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADQ2

PDB structure(s): 7q8s_B , 7q8s_G , 7q8s_H , 7q8s_I , 7q8s_J

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0007015	actin filament organization	5	11
GO:0008154	actin polymerization or depolymerization	6	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0022411	cellular component disassembly	4	11
GO:0030042	actin filament depolymerization	7	11
GO:0032984	protein-containing complex disassembly	5	11
GO:0043933	protein-containing complex organization	4	11
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051261	protein depolymerization	6	11
GO:0051301	cell division	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0071840	cellular component organization or biogenesis	2	11
GO:0097435	supramolecular fiber organization	4	11

Molecular functions
Term	Name	Level	Count
GO:0003779	actin binding	4	11
GO:0005488	binding	1	11
GO:0005515	protein binding	2	11
GO:0008092	cytoskeletal protein binding	3	11
GO:0044877	protein-containing complex binding	2	2
GO:0051015	actin filament binding	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	122	126	PF00656	0.617
CLV_C14_Caspase3-7	70	74	PF00656	0.503
CLV_NRD_NRD_1	130	132	PF00675	0.298
CLV_PCSK_KEX2_1	77	79	PF00082	0.324
CLV_PCSK_PC1ET2_1	77	79	PF00082	0.324
CLV_PCSK_SKI1_1	103	107	PF00082	0.285
CLV_PCSK_SKI1_1	80	84	PF00082	0.324
DEG_APCC_DBOX_1	102	110	PF00400	0.541
DEG_APCC_DBOX_1	77	85	PF00400	0.485
DOC_MAPK_gen_1	22	31	PF00069	0.488
DOC_MAPK_gen_1	77	84	PF00069	0.524
DOC_USP7_MATH_1	105	109	PF00917	0.629
LIG_14-3-3_CterR_2	134	139	PF00244	0.593
LIG_BIR_II_1	1	5	PF00653	0.538
LIG_FHA_2	5	11	PF00498	0.520
LIG_LIR_Gen_1	24	33	PF02991	0.501
LIG_LIR_Nem_3	110	116	PF02991	0.516
LIG_SH2_NCK_1	71	75	PF00017	0.524
LIG_SH2_SRC	113	116	PF00017	0.616
LIG_SH2_STAT5	113	116	PF00017	0.582
LIG_SH2_STAT5	26	29	PF00017	0.485
LIG_SH2_STAT5	50	53	PF00017	0.487
LIG_SH2_STAT5	64	67	PF00017	0.597
LIG_SUMO_SIM_anti_2	79	85	PF11976	0.485
LIG_TRAF2_1	7	10	PF00917	0.573
LIG_TRAF2_2	115	120	PF00917	0.516
LIG_TYR_ITIM	48	53	PF00017	0.487
LIG_UBA3_1	28	36	PF00899	0.482
MOD_CK1_1	107	113	PF00069	0.591
MOD_CK1_1	4	10	PF00069	0.447
MOD_CK2_1	105	111	PF00069	0.608
MOD_CK2_1	4	10	PF00069	0.602
MOD_CK2_1	73	79	PF00069	0.504
MOD_Cter_Amidation	34	37	PF01082	0.365
MOD_GlcNHglycan	120	124	PF01048	0.364
MOD_GlcNHglycan	3	6	PF01048	0.488
MOD_GlcNHglycan	57	60	PF01048	0.352
MOD_GlcNHglycan	99	102	PF01048	0.325
MOD_GSK3_1	4	11	PF00069	0.479
MOD_N-GLC_1	48	53	PF02516	0.413
MOD_NEK2_1	1	6	PF00069	0.532
MOD_NEK2_1	21	26	PF00069	0.392
MOD_NEK2_1	8	13	PF00069	0.448
MOD_NEK2_1	97	102	PF00069	0.494
MOD_Plk_1	8	14	PF00069	0.476
TRG_DiLeu_BaEn_1	79	84	PF01217	0.565
TRG_ENDOCYTIC_2	113	116	PF00928	0.616
TRG_ENDOCYTIC_2	26	29	PF00928	0.485
TRG_ENDOCYTIC_2	50	53	PF00928	0.541
TRG_ENDOCYTIC_2	64	67	PF00928	0.516
TRG_NLS_MonoExtN_4	20	26	PF00514	0.485

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I6H8	Leptomonas seymouri	74%	100%
A0A0S4JEQ6	Bodo saltans	42%	100%
A0A1X0NZK0	Trypanosomatidae	51%	100%
A0A3R7NX89	Trypanosoma rangeli	49%	100%
A0A3S7X2A7	Leishmania donovani	98%	100%
A4HH52	Leishmania braziliensis	76%	100%
A4I4A3	Leishmania infantum	97%	100%
C9ZKM6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
E9AM28	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
O49606	Arabidopsis thaliana	33%	99%
P0DJ26	Dictyostelium discoideum	28%	100%
P0DJ27	Dictyostelium discoideum	28%	100%
P10668	Sus scrofa	30%	84%
P18359	Gallus gallus	33%	84%
P18760	Mus musculus	30%	84%
P21566	Gallus gallus	31%	84%
P23528	Homo sapiens	30%	84%
P30174	Brassica napus	29%	100%
P30175	Lilium longiflorum	27%	100%
P37167	Acanthamoeba castellanii	27%	100%
P45591	Mus musculus	30%	84%
P45592	Rattus norvegicus	30%	84%
P45594	Drosophila melanogaster	37%	94%
P46251	Zea mays	27%	100%
P60981	Homo sapiens	33%	84%
P60982	Sus scrofa	33%	84%
P78929	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	100%
Q03048	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	32%	97%
Q07749	Caenorhabditis elegans	30%	91%
Q0DLA3	Oryza sativa subsp. japonica	31%	100%
Q10P87	Oryza sativa subsp. japonica	28%	97%
Q148F1	Bos taurus	30%	84%
Q2QLT8	Oryza sativa subsp. japonica	28%	96%
Q337A5	Oryza sativa subsp. japonica	28%	92%
Q39250	Arabidopsis thaliana	27%	100%
Q39251	Arabidopsis thaliana	29%	100%
Q41764	Zea mays	26%	100%
Q43694	Zea mays	28%	100%
Q4I963	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	29%	91%
Q4P6E9	Ustilago maydis (strain 521 / FGSC 9021)	29%	100%
Q4R5C0	Macaca fascicularis	30%	84%
Q570Y6	Arabidopsis thaliana	24%	99%
Q5E9D5	Bos taurus	33%	84%
Q5E9F7	Bos taurus	30%	84%
Q5G6V9	Sus scrofa	30%	84%
Q5U4Y2	Xenopus tropicalis	31%	83%
Q5XHH8	Xenopus laevis	31%	83%
Q67ZM4	Arabidopsis thaliana	29%	100%
Q6B7M7	Ovis aries	30%	84%
Q6BWX4	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	31%	97%
Q6C0Y0	Yarrowia lipolytica (strain CLIB 122 / E 150)	33%	91%
Q6CQ22	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	33%	97%
Q6EUH7	Oryza sativa subsp. japonica	29%	100%
Q6FV81	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	32%	97%
Q759P0	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	36%	97%
Q7M0E3	Rattus norvegicus	33%	84%
Q7XSN9	Oryza sativa subsp. japonica	28%	100%
Q84TB3	Oryza sativa subsp. japonica	28%	100%
Q84TB6	Oryza sativa subsp. japonica	25%	93%
Q8H2P8	Oryza sativa subsp. japonica	28%	100%
Q8LFH6	Arabidopsis thaliana	28%	100%
Q8SAG3	Vitis vinifera	26%	97%
Q96VU9	Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1)	32%	97%
Q9AY76	Oryza sativa subsp. japonica	31%	96%
Q9FVI1	Petunia hybrida	29%	97%
Q9FVI2	Petunia hybrida	29%	100%
Q9HF97	Zygosaccharomyces rouxii	34%	97%
Q9LQ81	Arabidopsis thaliana	24%	99%
Q9R0P5	Mus musculus	32%	84%
Q9Y281	Homo sapiens	30%	84%
Q9ZNT3	Arabidopsis thaliana	31%	97%
Q9ZSK2	Arabidopsis thaliana	29%	95%
Q9ZSK3	Arabidopsis thaliana	28%	100%
Q9ZSK4	Arabidopsis thaliana	30%	100%