Metallophos domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

kinetoplastid-specific phospho-protein phosphatase, putative

Species:

Leishmania major

UniProt:

E9ADP5_LEIMA

TriTrypDb:

LmjF.29.0440 , LMJLV39_290009700 , LMJSD75_290009700

Length:

380

Annotations

LeishMANIAdb annotations

A metalloenzyme with the catalytic domain facing outwards. Related to plant Shewanella-like protein phosphatases. Kinetoplastids have multiple copies of these genes but probably from a very ancient gene duplication.. The cluster might merge two separate, very distantly related groups.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 11, no: 11
NetGPI	no	yes: 0, no: 22

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	14
GO:0110165	cellular anatomical entity	1	14

Expansion

Sequence features

E9ADP5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADP5

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	23
GO:0016787	hydrolase activity	2	23

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	304	308	PF00656	0.224
CLV_NRD_NRD_1	117	119	PF00675	0.389
CLV_NRD_NRD_1	16	18	PF00675	0.457
CLV_NRD_NRD_1	232	234	PF00675	0.566
CLV_NRD_NRD_1	348	350	PF00675	0.707
CLV_NRD_NRD_1	4	6	PF00675	0.521
CLV_PCSK_KEX2_1	232	234	PF00082	0.505
CLV_PCSK_KEX2_1	348	350	PF00082	0.707
CLV_PCSK_PC1ET2_1	348	350	PF00082	0.523
CLV_PCSK_SKI1_1	158	162	PF00082	0.548
CLV_PCSK_SKI1_1	317	321	PF00082	0.434
CLV_PCSK_SKI1_1	345	349	PF00082	0.512
CLV_PCSK_SKI1_1	351	355	PF00082	0.649
CLV_PCSK_SKI1_1	371	375	PF00082	0.609
DEG_APCC_KENBOX_2	289	293	PF00400	0.189
DOC_ANK_TNKS_1	85	92	PF00023	0.300
DOC_CKS1_1	47	52	PF01111	0.373
DOC_CKS1_1	68	73	PF01111	0.356
DOC_CYCLIN_RxL_1	178	186	PF00134	0.204
DOC_CYCLIN_RxL_1	345	358	PF00134	0.252
DOC_MAPK_gen_1	118	126	PF00069	0.189
DOC_MAPK_gen_1	288	297	PF00069	0.348
DOC_MAPK_gen_1	345	354	PF00069	0.263
DOC_MAPK_gen_1	5	11	PF00069	0.557
DOC_MAPK_HePTP_8	287	299	PF00069	0.204
DOC_MAPK_MEF2A_6	119	128	PF00069	0.189
DOC_MAPK_MEF2A_6	290	299	PF00069	0.343
DOC_MAPK_MEF2A_6	317	325	PF00069	0.339
DOC_MAPK_RevD_3	336	351	PF00069	0.250
DOC_PP4_MxPP_1	1	4	PF00568	0.505
DOC_USP7_MATH_1	152	156	PF00917	0.315
DOC_USP7_MATH_1	214	218	PF00917	0.291
DOC_USP7_MATH_1	336	340	PF00917	0.317
DOC_USP7_MATH_1	63	67	PF00917	0.448
DOC_USP7_UBL2_3	346	350	PF12436	0.350
DOC_WW_Pin1_4	258	263	PF00397	0.251
DOC_WW_Pin1_4	46	51	PF00397	0.465
DOC_WW_Pin1_4	67	72	PF00397	0.378
LIG_14-3-3_CanoR_1	118	128	PF00244	0.214
LIG_14-3-3_CanoR_1	158	163	PF00244	0.223
LIG_14-3-3_CanoR_1	359	368	PF00244	0.367
LIG_14-3-3_CanoR_1	99	105	PF00244	0.222
LIG_Actin_WH2_2	90	107	PF00022	0.258
LIG_BIR_II_1	1	5	PF00653	0.646
LIG_BIR_III_1	1	5	PF00653	0.532
LIG_BIR_III_3	1	5	PF00653	0.532
LIG_BRCT_BRCA1_1	21	25	PF00533	0.514
LIG_Clathr_ClatBox_1	318	322	PF01394	0.257
LIG_eIF4E_1	243	249	PF01652	0.159
LIG_FHA_1	120	126	PF00498	0.277
LIG_FHA_1	318	324	PF00498	0.362
LIG_FHA_1	35	41	PF00498	0.310
LIG_FHA_1	47	53	PF00498	0.436
LIG_FHA_2	302	308	PF00498	0.381
LIG_LIR_Apic_2	176	180	PF02991	0.290
LIG_LIR_Gen_1	335	344	PF02991	0.332
LIG_LIR_Nem_3	186	192	PF02991	0.273
LIG_LIR_Nem_3	335	340	PF02991	0.348
LIG_PDZ_Class_1	375	380	PF00595	0.365
LIG_Pex14_2	34	38	PF04695	0.286
LIG_PTB_Apo_2	328	335	PF02174	0.255
LIG_PTB_Phospho_1	328	334	PF10480	0.259
LIG_SH2_PTP2	177	180	PF00017	0.241
LIG_SH2_PTP2	189	192	PF00017	0.241
LIG_SH2_SRC	177	180	PF00017	0.247
LIG_SH2_SRC	194	197	PF00017	0.329
LIG_SH2_STAP1	334	338	PF00017	0.345
LIG_SH2_STAT5	177	180	PF00017	0.264
LIG_SH2_STAT5	189	192	PF00017	0.269
LIG_SH2_STAT5	194	197	PF00017	0.265
LIG_SH2_STAT5	328	331	PF00017	0.365
LIG_SH2_STAT5	48	51	PF00017	0.502
LIG_SH3_3	133	139	PF00018	0.204
LIG_SH3_3	362	368	PF00018	0.289
LIG_SH3_3	65	71	PF00018	0.388
LIG_SUMO_SIM_anti_2	28	34	PF11976	0.460
LIG_SUMO_SIM_par_1	158	163	PF11976	0.204
LIG_SUMO_SIM_par_1	167	172	PF11976	0.175
LIG_SUMO_SIM_par_1	86	92	PF11976	0.281
LIG_TRFH_1	364	368	PF08558	0.249
LIG_UBA3_1	141	149	PF00899	0.243
LIG_WRC_WIRS_1	337	342	PF05994	0.227
LIG_WW_3	2	6	PF00397	0.499
MOD_CDK_SPxxK_3	70	77	PF00069	0.306
MOD_GlcNHglycan	145	148	PF01048	0.557
MOD_GlcNHglycan	154	157	PF01048	0.542
MOD_GlcNHglycan	162	165	PF01048	0.491
MOD_GlcNHglycan	171	174	PF01048	0.452
MOD_GlcNHglycan	185	188	PF01048	0.442
MOD_GlcNHglycan	21	24	PF01048	0.372
MOD_GlcNHglycan	280	283	PF01048	0.503
MOD_GSK3_1	34	41	PF00069	0.349
MOD_GSK3_1	63	70	PF00069	0.431
MOD_N-GLC_1	183	188	PF02516	0.520
MOD_NEK2_1	100	105	PF00069	0.288
MOD_NEK2_1	169	174	PF00069	0.336
MOD_NEK2_1	183	188	PF00069	0.268
MOD_NEK2_1	25	30	PF00069	0.353
MOD_NEK2_1	34	39	PF00069	0.333
MOD_NEK2_2	301	306	PF00069	0.209
MOD_PKA_2	100	106	PF00069	0.310
MOD_Plk_1	264	270	PF00069	0.349
MOD_Plk_4	264	270	PF00069	0.349
MOD_Plk_4	336	342	PF00069	0.210
MOD_Plk_4	34	40	PF00069	0.310
MOD_ProDKin_1	258	264	PF00069	0.251
MOD_ProDKin_1	46	52	PF00069	0.466
MOD_ProDKin_1	67	73	PF00069	0.364
TRG_DiLeu_BaEn_1	140	145	PF01217	0.189
TRG_DiLeu_BaEn_1	244	249	PF01217	0.243
TRG_DiLeu_BaLyEn_6	155	160	PF01217	0.369
TRG_ENDOCYTIC_2	189	192	PF00928	0.332
TRG_ENDOCYTIC_2	202	205	PF00928	0.369
TRG_ENDOCYTIC_2	334	337	PF00928	0.306
TRG_NLS_MonoCore_2	347	352	PF00514	0.297
TRG_NLS_MonoExtC_3	344	349	PF00514	0.283
TRG_NLS_MonoExtN_4	342	349	PF00514	0.284

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3V2	Leptomonas seymouri	29%	100%
A0A0N1P9R1	Leptomonas seymouri	26%	100%
A0A0S4JMN0	Bodo saltans	32%	94%
A0A0S4KJG1	Bodo saltans	27%	100%
A0A1X0NU01	Trypanosomatidae	27%	100%
A0A1X0NZX7	Trypanosomatidae	35%	85%
A0A1X0P2G6	Trypanosomatidae	30%	100%
A0A3Q8IBB4	Leishmania donovani	28%	100%
A0A3Q8IIK0	Leishmania donovani	94%	100%
A0A3R7NTC0	Trypanosoma rangeli	27%	100%
A0A3S5IRW3	Trypanosoma rangeli	34%	92%
A0A3S7X3U9	Leishmania donovani	28%	100%
A4HCJ2	Leishmania braziliensis	30%	100%
A4HH45	Leishmania braziliensis	74%	100%
A4HIR7	Leishmania braziliensis	29%	90%
A4I008	Leishmania infantum	28%	100%
A4I498	Leishmania infantum	94%	100%
A4I612	Leishmania infantum	28%	100%
C9ZQ86	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	100%
C9ZRD7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	86%
E9AM35	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
E9AVY1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9B1A3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
O74480	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
Q4Q6W1	Leishmania major	31%	100%
Q4QBJ8	Leishmania major	29%	100%
Q944L7	Arabidopsis thaliana	27%	97%
V5ARZ9	Trypanosoma cruzi	26%	100%
V5BPY7	Trypanosoma cruzi	33%	87%
V5BX32	Trypanosoma cruzi	30%	100%