RNA polymerase Rpb4 family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase Rpb4, putative

Species:

Leishmania major

UniProt:

E9ADP1_LEIMA

TriTrypDb:

LmjF.29.0400 , LMJLV39_290009200 , LMJSD75_290009200

Length:

166

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	4
GO:0000932	P-body	5	2
GO:0005654	nucleoplasm	2	2
GO:0005665	RNA polymerase II, core complex	4	2
GO:0030880	RNA polymerase complex	3	4
GO:0032991	protein-containing complex	1	4
GO:0035770	ribonucleoprotein granule	3	2
GO:0036464	cytoplasmic ribonucleoprotein granule	4	2
GO:0043226	organelle	2	11
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	11
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	2
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	4
GO:0099080	supramolecular complex	2	2
GO:0110165	cellular anatomical entity	1	11
GO:0140513	nuclear protein-containing complex	2	2
GO:0140535	intracellular protein-containing complex	2	4
GO:1902494	catalytic complex	2	4
GO:1990234	transferase complex	3	4
GO:0005634	nucleus	5	9
GO:0043227	membrane-bounded organelle	3	9
GO:0043231	intracellular membrane-bounded organelle	4	9

Expansion

Sequence features

E9ADP1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADP1

Function

Biological processes
Term	Name	Level	Count
GO:0000288	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	6	2
GO:0000956	nuclear-transcribed mRNA catabolic process	7	2
GO:0006139	nucleobase-containing compound metabolic process	3	3
GO:0006352	DNA-templated transcription initiation	6	3
GO:0006367	transcription initiation at RNA polymerase II promoter	7	2
GO:0006401	RNA catabolic process	5	2
GO:0006402	mRNA catabolic process	6	2
GO:0006405	RNA export from nucleus	5	2
GO:0006406	mRNA export from nucleus	6	2
GO:0006417	regulation of translation	6	2
GO:0006446	regulation of translational initiation	7	2
GO:0006725	cellular aromatic compound metabolic process	3	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0006810	transport	3	2
GO:0006913	nucleocytoplasmic transport	5	2
GO:0006950	response to stress	2	2
GO:0008152	metabolic process	1	3
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009058	biosynthetic process	2	3
GO:0009059	macromolecule biosynthetic process	4	3
GO:0009266	response to temperature stimulus	3	2
GO:0009408	response to heat	3	2
GO:0009628	response to abiotic stimulus	2	2
GO:0009889	regulation of biosynthetic process	4	2
GO:0009890	negative regulation of biosynthetic process	5	2
GO:0009891	positive regulation of biosynthetic process	5	2
GO:0009892	negative regulation of metabolic process	4	2
GO:0009893	positive regulation of metabolic process	4	2
GO:0009894	regulation of catabolic process	4	2
GO:0009896	positive regulation of catabolic process	5	2
GO:0009987	cellular process	1	3
GO:0010033	response to organic substance	3	2
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0010557	positive regulation of macromolecule biosynthetic process	6	2
GO:0010558	negative regulation of macromolecule biosynthetic process	6	2
GO:0010604	positive regulation of macromolecule metabolic process	5	2
GO:0010605	negative regulation of macromolecule metabolic process	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0010628	positive regulation of gene expression	6	2
GO:0010629	negative regulation of gene expression	6	2
GO:0014070	response to organic cyclic compound	4	2
GO:0015931	nucleobase-containing compound transport	5	2
GO:0016043	cellular component organization	3	2
GO:0016070	RNA metabolic process	5	3
GO:0016071	mRNA metabolic process	6	2
GO:0017148	negative regulation of translation	7	2
GO:0018130	heterocycle biosynthetic process	4	3
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0019438	aromatic compound biosynthetic process	4	3
GO:0019439	aromatic compound catabolic process	4	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031324	negative regulation of cellular metabolic process	5	2
GO:0031325	positive regulation of cellular metabolic process	5	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0031327	negative regulation of cellular biosynthetic process	6	2
GO:0031328	positive regulation of cellular biosynthetic process	6	2
GO:0031329	regulation of cellular catabolic process	5	2
GO:0031331	positive regulation of cellular catabolic process	6	2
GO:0031990	mRNA export from nucleus in response to heat stress	4	2
GO:0032774	RNA biosynthetic process	5	3
GO:0033554	cellular response to stress	3	2
GO:0034248	regulation of amide metabolic process	5	2
GO:0034249	negative regulation of amide metabolic process	6	2
GO:0034250	positive regulation of amide metabolic process	6	2
GO:0034402	recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex	7	2
GO:0034605	cellular response to heat	4	2
GO:0034641	cellular nitrogen compound metabolic process	3	3
GO:0034654	nucleobase-containing compound biosynthetic process	4	3
GO:0034655	nucleobase-containing compound catabolic process	4	2
GO:0042221	response to chemical	2	2
GO:0043170	macromolecule metabolic process	3	3
GO:0043487	regulation of RNA stability	3	2
GO:0043488	regulation of mRNA stability	4	2
GO:0043933	protein-containing complex organization	4	2
GO:0044237	cellular metabolic process	2	3
GO:0044238	primary metabolic process	2	3
GO:0044248	cellular catabolic process	3	2
GO:0044249	cellular biosynthetic process	3	3
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0044270	cellular nitrogen compound catabolic process	4	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	3
GO:0045727	positive regulation of translation	7	2
GO:0045935	positive regulation of nucleobase-containing compound metabolic process	6	2
GO:0045948	positive regulation of translational initiation	8	2
GO:0046483	heterocycle metabolic process	3	3
GO:0046700	heterocycle catabolic process	4	2
GO:0046907	intracellular transport	3	2
GO:0048518	positive regulation of biological process	3	2
GO:0048519	negative regulation of biological process	3	2
GO:0048522	positive regulation of cellular process	4	2
GO:0048523	negative regulation of cellular process	4	2
GO:0050657	nucleic acid transport	6	2
GO:0050658	RNA transport	4	2
GO:0050779	RNA destabilization	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050896	response to stimulus	1	2
GO:0051028	mRNA transport	5	2
GO:0051168	nuclear export	6	2
GO:0051169	nuclear transport	4	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051172	negative regulation of nitrogen compound metabolic process	5	2
GO:0051173	positive regulation of nitrogen compound metabolic process	5	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051236	establishment of RNA localization	3	2
GO:0051246	regulation of protein metabolic process	5	2
GO:0051247	positive regulation of protein metabolic process	6	2
GO:0051248	negative regulation of protein metabolic process	6	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0051254	positive regulation of RNA metabolic process	6	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0051716	cellular response to stimulus	2	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0061013	regulation of mRNA catabolic process	6	2
GO:0061014	positive regulation of mRNA catabolic process	7	2
GO:0061157	mRNA destabilization	5	2
GO:0065003	protein-containing complex assembly	5	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0071702	organic substance transport	4	2
GO:0071704	organic substance metabolic process	2	3
GO:0071705	nitrogen compound transport	4	2
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:0090304	nucleic acid metabolic process	4	3
GO:1901360	organic cyclic compound metabolic process	3	3
GO:1901361	organic cyclic compound catabolic process	4	2
GO:1901362	organic cyclic compound biosynthetic process	4	3
GO:1901575	organic substance catabolic process	3	2
GO:1901576	organic substance biosynthetic process	3	3
GO:1901698	response to nitrogen compound	3	2
GO:1903311	regulation of mRNA metabolic process	6	2
GO:1903313	positive regulation of mRNA metabolic process	7	2
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	2
GO:2000113	obsolete negative regulation of cellular macromolecule biosynthetic process	7	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0005488	binding	1	11
GO:0005515	protein binding	2	2
GO:0031369	translation initiation factor binding	3	2
GO:0036094	small molecule binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0003824	catalytic activity	1	1
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	1
GO:0016740	transferase activity	2	1
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	1
GO:0016779	nucleotidyltransferase activity	4	1
GO:0034062	5'-3' RNA polymerase activity	5	1
GO:0097747	RNA polymerase activity	4	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	75	81	PF00089	0.377
CLV_PCSK_KEX2_1	32	34	PF00082	0.485
CLV_PCSK_KEX2_1	73	75	PF00082	0.486
CLV_PCSK_PC1ET2_1	32	34	PF00082	0.577
CLV_PCSK_PC1ET2_1	73	75	PF00082	0.548
CLV_PCSK_SKI1_1	43	47	PF00082	0.567
DEG_Nend_UBRbox_2	1	3	PF02207	0.603
DOC_CYCLIN_yCln2_LP_2	20	26	PF00134	0.429
DOC_MAPK_gen_1	122	131	PF00069	0.463
DOC_MAPK_gen_1	73	79	PF00069	0.441
DOC_MAPK_RevD_3	20	33	PF00069	0.450
DOC_PP2B_LxvP_1	20	23	PF13499	0.431
DOC_USP7_MATH_1	113	117	PF00917	0.668
LIG_14-3-3_CanoR_1	135	143	PF00244	0.496
LIG_Actin_WH2_2	63	80	PF00022	0.503
LIG_CtBP_PxDLS_1	63	67	PF00389	0.505
LIG_FHA_1	37	43	PF00498	0.639
LIG_FHA_2	136	142	PF00498	0.442
LIG_LIR_Gen_1	96	104	PF02991	0.601
LIG_LIR_Nem_3	96	102	PF02991	0.644
LIG_SH2_CRK	99	103	PF00017	0.691
LIG_SH2_SRC	5	8	PF00017	0.494
LIG_SH2_STAT5	5	8	PF00017	0.494
LIG_SH3_3	98	104	PF00018	0.620
LIG_SUMO_SIM_anti_2	141	148	PF11976	0.443
LIG_SUMO_SIM_par_1	127	133	PF11976	0.427
MOD_CK1_1	115	121	PF00069	0.635
MOD_CK2_1	1	7	PF00069	0.645
MOD_CK2_1	115	121	PF00069	0.637
MOD_CK2_1	12	18	PF00069	0.619
MOD_CK2_1	135	141	PF00069	0.431
MOD_CK2_1	46	52	PF00069	0.521
MOD_Cter_Amidation	71	74	PF01082	0.487
MOD_GlcNHglycan	109	112	PF01048	0.689
MOD_GSK3_1	113	120	PF00069	0.630
MOD_GSK3_1	130	137	PF00069	0.535
MOD_GSK3_1	62	69	PF00069	0.550
MOD_NEK2_1	1	6	PF00069	0.614
MOD_NEK2_1	46	51	PF00069	0.515
MOD_NEK2_1	66	71	PF00069	0.465
MOD_NEK2_2	130	135	PF00069	0.496
MOD_PKA_2	134	140	PF00069	0.528
MOD_Plk_1	1	7	PF00069	0.612
MOD_Plk_4	1	7	PF00069	0.612
MOD_Plk_4	145	151	PF00069	0.497
MOD_Plk_4	62	68	PF00069	0.549
TRG_DiLeu_BaEn_1	141	146	PF01217	0.422
TRG_ENDOCYTIC_2	99	102	PF00928	0.657
TRG_ER_diArg_1	74	77	PF00400	0.456
TRG_Pf-PMV_PEXEL_1	27	31	PF00026	0.560

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1ILS1	Leptomonas seymouri	73%	100%
A0A0S4KR23	Bodo saltans	37%	100%
A0A1X0NZH4	Trypanosomatidae	39%	99%
A0A3S5H7K6	Leishmania donovani	94%	100%
A0A422NUI9	Trypanosoma rangeli	38%	98%
A4HH41	Leishmania braziliensis	89%	100%
A4I494	Leishmania infantum	95%	100%
C9ZKN9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
E9AM39	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%