Tubulin-tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Tubulin-tyrosine ligase family, putative

Species:

Leishmania major

UniProt:

E9ADM8_LEIMA

TriTrypDb:

LmjF.29.0260 , LMJLV39_290007700 , LMJSD75_290007700

Length:

617

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 17
NetGPI	no	yes: 0, no: 17

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	2
GO:0042995	cell projection	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0110165	cellular anatomical entity	1	2
GO:0120025	plasma membrane bounded cell projection	3	2

Expansion

Sequence features

E9ADM8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADM8

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	2
GO:0006807	nitrogen compound metabolic process	2	18
GO:0006996	organelle organization	4	2
GO:0007010	cytoskeleton organization	5	2
GO:0007017	microtubule-based process	2	2
GO:0008152	metabolic process	1	18
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0018095	protein polyglutamylation	7	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018200	peptidyl-glutamic acid modification	6	2
GO:0019538	protein metabolic process	3	18
GO:0036211	protein modification process	4	18
GO:0043170	macromolecule metabolic process	3	18
GO:0043412	macromolecule modification	4	18
GO:0044238	primary metabolic process	2	18
GO:0071704	organic substance metabolic process	2	18
GO:0071840	cellular component organization or biogenesis	2	2
GO:1901564	organonitrogen compound metabolic process	3	18

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	3
GO:0005515	protein binding	2	2
GO:0008092	cytoskeletal protein binding	3	2
GO:0015631	tubulin binding	4	2
GO:0016874	ligase activity	2	9
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	4
GO:0016881	acid-amino acid ligase activity	4	4
GO:0070739	protein-glutamic acid ligase activity	3	2
GO:0070740	tubulin-glutamic acid ligase activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	4
GO:0004835	tubulin-tyrosine ligase activity	3	2
GO:0000166	nucleotide binding	3	1
GO:0005524	ATP binding	5	1
GO:0017076	purine nucleotide binding	4	1
GO:0030554	adenyl nucleotide binding	5	1
GO:0032553	ribonucleotide binding	3	1
GO:0032555	purine ribonucleotide binding	4	1
GO:0032559	adenyl ribonucleotide binding	5	1
GO:0035639	purine ribonucleoside triphosphate binding	4	1
GO:0036094	small molecule binding	2	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1
GO:0097159	organic cyclic compound binding	2	1
GO:0097367	carbohydrate derivative binding	2	1
GO:1901265	nucleoside phosphate binding	3	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	143	145	PF00675	0.259
CLV_NRD_NRD_1	150	152	PF00675	0.259
CLV_NRD_NRD_1	210	212	PF00675	0.313
CLV_NRD_NRD_1	256	258	PF00675	0.332
CLV_NRD_NRD_1	277	279	PF00675	0.271
CLV_NRD_NRD_1	298	300	PF00675	0.608
CLV_NRD_NRD_1	435	437	PF00675	0.241
CLV_NRD_NRD_1	454	456	PF00675	0.283
CLV_NRD_NRD_1	571	573	PF00675	0.421
CLV_NRD_NRD_1	614	616	PF00675	0.525
CLV_PCSK_FUR_1	165	169	PF00082	0.284
CLV_PCSK_FUR_1	433	437	PF00082	0.219
CLV_PCSK_KEX2_1	150	152	PF00082	0.274
CLV_PCSK_KEX2_1	167	169	PF00082	0.307
CLV_PCSK_KEX2_1	210	212	PF00082	0.313
CLV_PCSK_KEX2_1	256	258	PF00082	0.337
CLV_PCSK_KEX2_1	260	262	PF00082	0.321
CLV_PCSK_KEX2_1	298	300	PF00082	0.607
CLV_PCSK_KEX2_1	435	437	PF00082	0.245
CLV_PCSK_KEX2_1	454	456	PF00082	0.316
CLV_PCSK_KEX2_1	571	573	PF00082	0.421
CLV_PCSK_KEX2_1	614	616	PF00082	0.515
CLV_PCSK_PC1ET2_1	167	169	PF00082	0.334
CLV_PCSK_PC1ET2_1	260	262	PF00082	0.331
CLV_PCSK_PC7_1	256	262	PF00082	0.332
CLV_PCSK_PC7_1	294	300	PF00082	0.477
CLV_PCSK_SKI1_1	102	106	PF00082	0.576
CLV_PCSK_SKI1_1	144	148	PF00082	0.239
CLV_PCSK_SKI1_1	224	228	PF00082	0.285
CLV_PCSK_SKI1_1	271	275	PF00082	0.332
CLV_PCSK_SKI1_1	365	369	PF00082	0.262
CLV_PCSK_SKI1_1	571	575	PF00082	0.431
CLV_Separin_Metazoa	327	331	PF03568	0.391
CLV_Separin_Metazoa	451	455	PF03568	0.449
DEG_APCC_DBOX_1	12	20	PF00400	0.491
DEG_APCC_DBOX_1	340	348	PF00400	0.507
DEG_Nend_Nbox_1	1	3	PF02207	0.705
DOC_CKS1_1	103	108	PF01111	0.416
DOC_CYCLIN_RxL_1	184	192	PF00134	0.419
DOC_CYCLIN_yCln2_LP_2	191	197	PF00134	0.449
DOC_MAPK_DCC_7	484	493	PF00069	0.532
DOC_MAPK_gen_1	11	19	PF00069	0.487
DOC_MAPK_gen_1	256	267	PF00069	0.508
DOC_MAPK_gen_1	278	286	PF00069	0.469
DOC_MAPK_MEF2A_6	260	269	PF00069	0.486
DOC_MAPK_MEF2A_6	278	286	PF00069	0.343
DOC_MAPK_MEF2A_6	330	339	PF00069	0.299
DOC_MAPK_NFAT4_5	279	287	PF00069	0.532
DOC_PP1_RVXF_1	143	150	PF00149	0.449
DOC_PP1_RVXF_1	222	229	PF00149	0.449
DOC_PP1_RVXF_1	71	78	PF00149	0.487
DOC_PP2B_LxvP_1	191	194	PF13499	0.449
DOC_PP2B_LxvP_1	378	381	PF13499	0.449
DOC_PP2B_LxvP_1	425	428	PF13499	0.419
DOC_USP7_MATH_1	217	221	PF00917	0.531
DOC_USP7_MATH_1	304	308	PF00917	0.678
DOC_USP7_MATH_1	398	402	PF00917	0.515
DOC_USP7_MATH_1	41	45	PF00917	0.735
DOC_USP7_MATH_1	417	421	PF00917	0.540
DOC_USP7_MATH_1	510	514	PF00917	0.522
DOC_WW_Pin1_4	1	6	PF00397	0.443
DOC_WW_Pin1_4	102	107	PF00397	0.565
DOC_WW_Pin1_4	243	248	PF00397	0.447
DOC_WW_Pin1_4	300	305	PF00397	0.732
DOC_WW_Pin1_4	394	399	PF00397	0.540
DOC_WW_Pin1_4	495	500	PF00397	0.431
DOC_WW_Pin1_4	533	538	PF00397	0.530
DOC_WW_Pin1_4	96	101	PF00397	0.443
LIG_14-3-3_CanoR_1	242	247	PF00244	0.511
LIG_14-3-3_CanoR_1	298	304	PF00244	0.588
LIG_14-3-3_CanoR_1	433	439	PF00244	0.539
LIG_Actin_WH2_2	504	519	PF00022	0.505
LIG_APCC_ABBA_1	23	28	PF00400	0.482
LIG_APCC_ABBA_1	265	270	PF00400	0.532
LIG_APCC_ABBA_1	337	342	PF00400	0.459
LIG_Clathr_ClatBox_1	476	480	PF01394	0.476
LIG_eIF4E_1	268	274	PF01652	0.513
LIG_EVH1_2	88	92	PF00568	0.371
LIG_FHA_1	176	182	PF00498	0.555
LIG_FHA_1	373	379	PF00498	0.537
LIG_FHA_1	441	447	PF00498	0.522
LIG_FHA_1	517	523	PF00498	0.510
LIG_FHA_2	232	238	PF00498	0.532
LIG_FHA_2	243	249	PF00498	0.532
LIG_FHA_2	250	256	PF00498	0.480
LIG_FHA_2	503	509	PF00498	0.462
LIG_FHA_2	76	82	PF00498	0.388
LIG_LIR_Apic_2	350	355	PF02991	0.400
LIG_LIR_Gen_1	186	195	PF02991	0.519
LIG_LIR_Gen_1	196	203	PF02991	0.476
LIG_LIR_Gen_1	472	482	PF02991	0.534
LIG_LIR_Gen_1	62	72	PF02991	0.374
LIG_LIR_Nem_3	186	191	PF02991	0.519
LIG_LIR_Nem_3	196	200	PF02991	0.476
LIG_LIR_Nem_3	472	477	PF02991	0.532
LIG_LIR_Nem_3	518	523	PF02991	0.443
LIG_LIR_Nem_3	62	68	PF02991	0.383
LIG_LIR_Nem_3	76	80	PF02991	0.433
LIG_NRBOX	283	289	PF00104	0.449
LIG_REV1ctd_RIR_1	225	231	PF16727	0.480
LIG_SH2_CRK	130	134	PF00017	0.530
LIG_SH2_CRK	22	26	PF00017	0.661
LIG_SH2_CRK	352	356	PF00017	0.437
LIG_SH2_CRK	65	69	PF00017	0.320
LIG_SH2_NCK_1	352	356	PF00017	0.437
LIG_SH2_PTP2	285	288	PF00017	0.488
LIG_SH2_SRC	379	382	PF00017	0.449
LIG_SH2_STAP1	540	544	PF00017	0.406
LIG_SH2_STAT5	2	5	PF00017	0.574
LIG_SH2_STAT5	268	271	PF00017	0.455
LIG_SH2_STAT5	285	288	PF00017	0.451
LIG_SH2_STAT5	338	341	PF00017	0.488
LIG_SH2_STAT5	379	382	PF00017	0.506
LIG_SH3_3	100	106	PF00018	0.488
LIG_SH3_3	266	272	PF00018	0.428
LIG_SH3_3	384	390	PF00018	0.360
LIG_SUMO_SIM_anti_2	443	448	PF11976	0.441
LIG_SUMO_SIM_par_1	442	448	PF11976	0.432
LIG_SUMO_SIM_par_1	608	613	PF11976	0.386
LIG_TYR_ITSM	61	68	PF00017	0.373
LIG_WRC_WIRS_1	418	423	PF05994	0.522
MOD_CDC14_SPxK_1	536	539	PF00782	0.301
MOD_CDC14_SPxK_1	99	102	PF00782	0.491
MOD_CDK_SPK_2	394	399	PF00069	0.360
MOD_CDK_SPxK_1	533	539	PF00069	0.293
MOD_CDK_SPxK_1	96	102	PF00069	0.452
MOD_CK1_1	160	166	PF00069	0.497
MOD_CK1_1	196	202	PF00069	0.435
MOD_CK1_1	297	303	PF00069	0.698
MOD_CK1_1	311	317	PF00069	0.531
MOD_CK1_1	323	329	PF00069	0.500
MOD_CK1_1	96	102	PF00069	0.663
MOD_CK2_1	180	186	PF00069	0.559
MOD_CK2_1	231	237	PF00069	0.559
MOD_CK2_1	242	248	PF00069	0.502
MOD_CK2_1	249	255	PF00069	0.413
MOD_CK2_1	417	423	PF00069	0.535
MOD_CK2_1	434	440	PF00069	0.493
MOD_CK2_1	445	451	PF00069	0.413
MOD_CK2_1	469	475	PF00069	0.449
MOD_CK2_1	79	85	PF00069	0.590
MOD_Cter_Amidation	276	279	PF01082	0.299
MOD_DYRK1A_RPxSP_1	102	106	PF00069	0.451
MOD_GlcNHglycan	159	162	PF01048	0.284
MOD_GlcNHglycan	315	318	PF01048	0.535
MOD_GlcNHglycan	43	46	PF01048	0.732
MOD_GlcNHglycan	447	450	PF01048	0.228
MOD_GlcNHglycan	51	55	PF01048	0.522
MOD_GlcNHglycan	512	515	PF01048	0.300
MOD_GlcNHglycan	577	580	PF01048	0.581
MOD_GSK3_1	115	122	PF00069	0.413
MOD_GSK3_1	153	160	PF00069	0.489
MOD_GSK3_1	189	196	PF00069	0.456
MOD_GSK3_1	294	301	PF00069	0.646
MOD_GSK3_1	304	311	PF00069	0.614
MOD_GSK3_1	316	323	PF00069	0.638
MOD_GSK3_1	361	368	PF00069	0.486
MOD_GSK3_1	394	401	PF00069	0.516
MOD_GSK3_1	407	414	PF00069	0.506
MOD_GSK3_1	417	424	PF00069	0.521
MOD_GSK3_1	75	82	PF00069	0.453
MOD_N-GLC_1	323	328	PF02516	0.626
MOD_N-GLC_1	372	377	PF02516	0.274
MOD_N-GLC_1	495	500	PF02516	0.237
MOD_NEK2_1	153	158	PF00069	0.492
MOD_NEK2_1	189	194	PF00069	0.522
MOD_NEK2_1	421	426	PF00069	0.539
MOD_NEK2_1	502	507	PF00069	0.449
MOD_NEK2_1	516	521	PF00069	0.451
MOD_NEK2_1	522	527	PF00069	0.324
MOD_PKA_1	298	304	PF00069	0.588
MOD_PKA_2	241	247	PF00069	0.513
MOD_PKA_2	297	303	PF00069	0.579
MOD_PKA_2	398	404	PF00069	0.360
MOD_PKA_2	434	440	PF00069	0.537
MOD_PKA_2	516	522	PF00069	0.449
MOD_PKA_2	60	66	PF00069	0.507
MOD_Plk_1	115	121	PF00069	0.366
MOD_Plk_1	181	187	PF00069	0.419
MOD_Plk_1	308	314	PF00069	0.487
MOD_Plk_1	75	81	PF00069	0.515
MOD_Plk_2-3	81	87	PF00069	0.565
MOD_Plk_4	115	121	PF00069	0.408
MOD_Plk_4	374	380	PF00069	0.534
MOD_Plk_4	417	423	PF00069	0.532
MOD_Plk_4	442	448	PF00069	0.467
MOD_Plk_4	497	503	PF00069	0.437
MOD_ProDKin_1	1	7	PF00069	0.438
MOD_ProDKin_1	243	249	PF00069	0.447
MOD_ProDKin_1	300	306	PF00069	0.733
MOD_ProDKin_1	394	400	PF00069	0.540
MOD_ProDKin_1	495	501	PF00069	0.431
MOD_ProDKin_1	533	539	PF00069	0.532
MOD_ProDKin_1	96	102	PF00069	0.452
MOD_SUMO_rev_2	541	550	PF00179	0.436
TRG_DiLeu_BaLyEn_6	269	274	PF01217	0.513
TRG_DiLeu_BaLyEn_6	518	523	PF01217	0.462
TRG_DiLeu_BaLyEn_6	569	574	PF01217	0.481
TRG_ENDOCYTIC_2	130	133	PF00928	0.513
TRG_ENDOCYTIC_2	136	139	PF00928	0.513
TRG_ENDOCYTIC_2	22	25	PF00928	0.649
TRG_ENDOCYTIC_2	285	288	PF00928	0.459
TRG_ENDOCYTIC_2	65	68	PF00928	0.340
TRG_ER_diArg_1	120	123	PF00400	0.431
TRG_ER_diArg_1	149	151	PF00400	0.492
TRG_ER_diArg_1	209	211	PF00400	0.513
TRG_ER_diArg_1	432	435	PF00400	0.464
TRG_ER_diArg_1	453	455	PF00400	0.527
TRG_ER_diArg_1	570	572	PF00400	0.418
TRG_ER_diArg_1	614	616	PF00400	0.559
TRG_NES_CRM1_1	281	295	PF08389	0.472
TRG_NES_CRM1_1	440	456	PF08389	0.449
TRG_Pf-PMV_PEXEL_1	150	154	PF00026	0.311
TRG_Pf-PMV_PEXEL_1	211	215	PF00026	0.257
TRG_Pf-PMV_PEXEL_1	436	440	PF00026	0.332

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7G7	Leptomonas seymouri	22%	75%
A0A0N1I1Q6	Leptomonas seymouri	27%	86%
A0A0N1IAP5	Leptomonas seymouri	25%	83%
A0A0N1IH77	Leptomonas seymouri	54%	100%
A0A0S4J7C9	Bodo saltans	26%	93%
A0A0S4JH38	Bodo saltans	32%	100%
A0A0S4KQK8	Bodo saltans	28%	94%
A0A1X0P0Y5	Trypanosomatidae	36%	100%
A0A1X0P6A0	Trypanosomatidae	26%	93%
A0A3Q8IDL7	Leishmania donovani	23%	100%
A0A3Q8III2	Leishmania donovani	27%	85%
A0A3R7MY56	Trypanosoma rangeli	22%	100%
A0A3S7X261	Leishmania donovani	93%	100%
A0A422N064	Trypanosoma rangeli	41%	100%
A0A422P136	Trypanosoma rangeli	25%	94%
A4HH59	Leishmania braziliensis	76%	97%
A4HYN9	Leishmania infantum	23%	100%
A4I479	Leishmania infantum	92%	100%
A4IC28	Leishmania infantum	27%	85%
A4Q9E8	Mus musculus	25%	75%
A4Q9F6	Mus musculus	26%	77%
A6NNM8	Homo sapiens	25%	76%
A8CVX7	Danio rerio	27%	79%
B6DTF7	Bodo saltans	23%	100%
C9ZI96	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	90%
C9ZKP9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
C9ZPR8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	100%
C9ZYH1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	94%
D0A044	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	22%	100%
E9AFW7	Leishmania major	26%	85%
E9AID0	Leishmania braziliensis	24%	100%
E9AM52	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9B720	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	85%
Q8N841	Homo sapiens	26%	73%
Q9BWV7	Homo sapiens	23%	100%
V5ARK1	Trypanosoma cruzi	26%	100%
V5B4U2	Trypanosoma cruzi	41%	100%