Plastid-encoded RNA polymerase subunit alpha

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerase II subunit 3, putative

Species:

Leishmania major

UniProt:

E9ADL6_LEIMA

TriTrypDb:

LmjF.29.0140 , LMJLV39_290006400 , LMJSD75_290006400

Length:

337

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0005665	RNA polymerase II, core complex	4	2
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	2
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0140513	nuclear protein-containing complex	2	2
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12

Expansion

Sequence features

E9ADL6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADL6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006351	DNA-templated transcription	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009059	macromolecule biosynthetic process	4	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0032774	RNA biosynthetic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:0097659	nucleic acid-templated transcription	6	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	6
GO:0003677	DNA binding	4	6
GO:0003824	catalytic activity	1	12
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	12
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0034062	5'-3' RNA polymerase activity	5	12
GO:0046983	protein dimerization activity	3	12
GO:0097159	organic cyclic compound binding	2	6
GO:0097747	RNA polymerase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	24	28	PF00656	0.523
CLV_NRD_NRD_1	156	158	PF00675	0.251
CLV_NRD_NRD_1	283	285	PF00675	0.264
CLV_NRD_NRD_1	323	325	PF00675	0.718
CLV_NRD_NRD_1	35	37	PF00675	0.251
CLV_PCSK_KEX2_1	35	37	PF00082	0.251
CLV_PCSK_KEX2_1	79	81	PF00082	0.271
CLV_PCSK_PC1ET2_1	79	81	PF00082	0.328
CLV_PCSK_SKI1_1	114	118	PF00082	0.356
CLV_PCSK_SKI1_1	205	209	PF00082	0.263
CLV_PCSK_SKI1_1	36	40	PF00082	0.253
DEG_APCC_DBOX_1	283	291	PF00400	0.463
DEG_APCC_DBOX_1	35	43	PF00400	0.451
DEG_APCC_DBOX_1	66	74	PF00400	0.451
DEG_Nend_UBRbox_1	1	4	PF02207	0.424
DOC_CDC14_PxL_1	39	47	PF14671	0.451
DOC_CYCLIN_RxL_1	76	86	PF00134	0.537
DOC_CYCLIN_yCln2_LP_2	241	247	PF00134	0.476
DOC_MAPK_MEF2A_6	146	154	PF00069	0.463
DOC_MAPK_MEF2A_6	176	183	PF00069	0.499
DOC_MAPK_MEF2A_6	240	248	PF00069	0.492
DOC_MAPK_RevD_3	270	286	PF00069	0.463
DOC_PP1_RVXF_1	77	84	PF00149	0.537
DOC_USP7_UBL2_3	158	162	PF12436	0.451
LIG_14-3-3_CanoR_1	289	295	PF00244	0.617
LIG_14-3-3_CanoR_1	53	59	PF00244	0.529
LIG_Actin_WH2_2	19	37	PF00022	0.319
LIG_APCC_ABBA_1	71	76	PF00400	0.529
LIG_FHA_1	18	24	PF00498	0.451
LIG_FHA_1	212	218	PF00498	0.480
LIG_FHA_1	259	265	PF00498	0.451
LIG_FHA_1	298	304	PF00498	0.611
LIG_FHA_1	311	317	PF00498	0.570
LIG_FHA_1	42	48	PF00498	0.473
LIG_FHA_1	54	60	PF00498	0.414
LIG_FHA_1	98	104	PF00498	0.482
LIG_FHA_2	230	236	PF00498	0.537
LIG_LIR_Gen_1	235	246	PF02991	0.476
LIG_LIR_Nem_3	235	241	PF02991	0.476
LIG_LIR_Nem_3	48	52	PF02991	0.556
LIG_Pex14_2	258	262	PF04695	0.537
LIG_SH2_CRK	277	281	PF00017	0.537
LIG_SH2_NCK_1	277	281	PF00017	0.476
LIG_SH2_PTP2	49	52	PF00017	0.556
LIG_SH2_STAP1	277	281	PF00017	0.523
LIG_SH2_STAT5	257	260	PF00017	0.536
LIG_SH2_STAT5	49	52	PF00017	0.556
LIG_SH3_3	102	108	PF00018	0.466
LIG_SH3_3	312	318	PF00018	0.565
LIG_SUMO_SIM_anti_2	278	284	PF11976	0.463
LIG_SUMO_SIM_par_1	43	48	PF11976	0.537
LIG_TRAF2_1	133	136	PF00917	0.463
LIG_TRAF2_1	232	235	PF00917	0.537
LIG_TYR_ITIM	275	280	PF00017	0.476
LIG_TYR_ITIM	47	52	PF00017	0.556
MOD_CK2_1	130	136	PF00069	0.451
MOD_CK2_1	229	235	PF00069	0.522
MOD_GlcNHglycan	132	135	PF01048	0.335
MOD_GlcNHglycan	91	94	PF01048	0.253
MOD_GSK3_1	140	147	PF00069	0.461
MOD_GSK3_1	17	24	PF00069	0.333
MOD_GSK3_1	260	267	PF00069	0.446
MOD_GSK3_1	304	311	PF00069	0.700
MOD_N-GLC_1	54	59	PF02516	0.251
MOD_NEK2_1	144	149	PF00069	0.537
MOD_NEK2_1	258	263	PF00069	0.512
MOD_NEK2_1	332	337	PF00069	0.714
MOD_PIKK_1	144	150	PF00454	0.463
MOD_PIKK_1	230	236	PF00454	0.537
MOD_PKA_2	288	294	PF00069	0.601
MOD_Plk_1	258	264	PF00069	0.463
MOD_Plk_1	26	32	PF00069	0.451
MOD_Plk_1	54	60	PF00069	0.451
MOD_Plk_4	54	60	PF00069	0.454
MOD_SUMO_for_1	9	12	PF00179	0.555
TRG_DiLeu_BaEn_2	242	248	PF01217	0.476
TRG_ENDOCYTIC_2	257	260	PF00928	0.475
TRG_ENDOCYTIC_2	277	280	PF00928	0.476
TRG_ENDOCYTIC_2	49	52	PF00928	0.556
TRG_ER_diArg_1	34	36	PF00400	0.451
TRG_NES_CRM1_1	62	76	PF08389	0.529
TRG_NLS_MonoExtN_4	249	255	PF00514	0.556
TRG_Pf-PMV_PEXEL_1	306	310	PF00026	0.641

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8L4	Leptomonas seymouri	26%	76%
A0A0N1I3K4	Leptomonas seymouri	88%	100%
A0A0S4IYI3	Bodo saltans	30%	93%
A0A0S4JFX1	Bodo saltans	49%	100%
A0A1X0NZD0	Trypanosomatidae	68%	100%
A0A1X0P6H8	Trypanosomatidae	28%	84%
A0A3R7MJ28	Trypanosoma rangeli	30%	86%
A0A3S5H758	Leishmania donovani	27%	76%
A0A3S7X218	Leishmania donovani	99%	100%
A0A422NUV2	Trypanosoma rangeli	69%	100%
A3MXZ5	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	28%	100%
A4HA24	Leishmania braziliensis	26%	76%
A4HH22	Leishmania braziliensis	93%	100%
A4HY87	Leishmania infantum	27%	76%
A4I451	Leishmania infantum	99%	100%
A4WNA4	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	28%	100%
A5UN55	Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)	28%	100%
B0R4Y2	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	25%	100%
B1YC30	Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta)	29%	100%
B8YB56	Saccharolobus shibatae	28%	100%
C3MJP7	Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)	28%	100%
C3MZ05	Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)	28%	100%
C3N054	Sulfolobus islandicus (strain M.16.27)	28%	100%
C3N8R8	Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)	28%	100%
C3NMQ0	Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)	28%	100%
C4KJ93	Sulfolobus islandicus (strain M.16.4 / Kamchatka #3)	28%	100%
C9ZKR1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
E9AM65	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
E9AS18	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	78%
O15160	Homo sapiens	29%	97%
O26144	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	30%	100%
O28002	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	34%	100%
O59303	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	30%	100%
O94616	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	97%
P07703	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
P0CG28	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	29%	100%
P16370	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
P16925	Tetrahymena thermophila	34%	100%
P19387	Homo sapiens	30%	100%
P37382	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
P39471	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	28%	100%
P52432	Mus musculus	29%	97%
P95989	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	27%	100%
P97760	Mus musculus	30%	100%
Q00813	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	25%	100%
Q2NFZ6	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	30%	100%
Q32L22	Bos taurus	30%	97%
Q39211	Arabidopsis thaliana	38%	100%
Q39212	Arabidopsis thaliana	37%	100%
Q3IQT6	Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)	26%	100%
Q3T0Q3	Bos taurus	30%	100%
Q4QDF5	Leishmania major	27%	76%
Q54DH7	Dictyostelium discoideum	37%	100%
Q54SN4	Dictyostelium discoideum	31%	98%
Q5JJF4	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	29%	100%
Q6KZP5	Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)	25%	100%
Q8PV16	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	28%	100%
Q8TVB8	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	29%	100%
Q8ZYQ3	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	28%	100%
Q96YW0	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	28%	100%
Q9HQJ4	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	25%	100%
Q9V198	Pyrococcus abyssi (strain GE5 / Orsay)	30%	100%
V5BBX7	Trypanosoma cruzi	30%	86%
V5BUI6	Trypanosoma cruzi	68%	100%