Putative proteasome regulatory non-ATPase subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

proteasome regulatory non-ATPase subunit, putative

Species:

Leishmania major

UniProt:

E9ADL4_LEIMA

TriTrypDb:

LmjF.29.0120 , LMJLV39_290006200 , LMJSD75_290006200

Length:

909

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 5

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	2
GO:0008540	proteasome regulatory particle, base subcomplex	2	2
GO:0032991	protein-containing complex	1	12
GO:0034515	proteasome storage granule	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2
GO:0000502	proteasome complex	3	10
GO:0140535	intracellular protein-containing complex	2	10
GO:1902494	catalytic complex	2	10
GO:1905368	peptidase complex	3	10
GO:1905369	endopeptidase complex	4	10

Expansion

Sequence features

E9ADL4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADL4

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009894	regulation of catabolic process	4	12
GO:0009987	cellular process	1	2
GO:0010498	proteasomal protein catabolic process	5	2
GO:0019222	regulation of metabolic process	3	12
GO:0019538	protein metabolic process	3	2
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0030163	protein catabolic process	4	2
GO:0042176	regulation of protein catabolic process	5	12
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0050789	regulation of biological process	2	12
GO:0051171	regulation of nitrogen compound metabolic process	4	12
GO:0051246	regulation of protein metabolic process	5	12
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0060255	regulation of macromolecule metabolic process	4	12
GO:0065007	biological regulation	1	12
GO:0071704	organic substance metabolic process	2	2
GO:0080090	regulation of primary metabolic process	4	12
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901565	organonitrogen compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0030234	enzyme regulator activity	2	12
GO:0098772	molecular function regulator activity	1	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	559	563	PF00656	0.453
CLV_MEL_PAP_1	844	850	PF00089	0.433
CLV_NRD_NRD_1	111	113	PF00675	0.397
CLV_NRD_NRD_1	476	478	PF00675	0.455
CLV_PCSK_KEX2_1	111	113	PF00082	0.399
CLV_PCSK_KEX2_1	220	222	PF00082	0.324
CLV_PCSK_KEX2_1	664	666	PF00082	0.436
CLV_PCSK_PC1ET2_1	220	222	PF00082	0.455
CLV_PCSK_PC1ET2_1	664	666	PF00082	0.436
CLV_PCSK_SKI1_1	131	135	PF00082	0.369
CLV_PCSK_SKI1_1	19	23	PF00082	0.642
CLV_PCSK_SKI1_1	231	235	PF00082	0.347
CLV_PCSK_SKI1_1	295	299	PF00082	0.393
CLV_PCSK_SKI1_1	482	486	PF00082	0.324
CLV_PCSK_SKI1_1	739	743	PF00082	0.506
CLV_PCSK_SKI1_1	753	757	PF00082	0.488
CLV_PCSK_SKI1_1	862	866	PF00082	0.309
DEG_APCC_KENBOX_2	28	32	PF00400	0.694
DEG_Nend_UBRbox_2	1	3	PF02207	0.694
DEG_SCF_FBW7_1	822	827	PF00400	0.488
DOC_CKS1_1	870	875	PF01111	0.369
DOC_CYCLIN_RxL_1	206	217	PF00134	0.374
DOC_CYCLIN_yCln2_LP_2	484	490	PF00134	0.343
DOC_MAPK_gen_1	111	119	PF00069	0.384
DOC_MAPK_gen_1	281	289	PF00069	0.402
DOC_MAPK_gen_1	293	300	PF00069	0.394
DOC_MAPK_gen_1	4	12	PF00069	0.659
DOC_MAPK_gen_1	41	50	PF00069	0.538
DOC_MAPK_HePTP_8	639	651	PF00069	0.434
DOC_MAPK_JIP1_4	281	287	PF00069	0.455
DOC_MAPK_MEF2A_6	4	12	PF00069	0.636
DOC_MAPK_MEF2A_6	41	50	PF00069	0.492
DOC_MAPK_MEF2A_6	440	449	PF00069	0.593
DOC_MAPK_MEF2A_6	642	651	PF00069	0.405
DOC_PP2B_LxvP_1	484	487	PF13499	0.324
DOC_USP7_MATH_1	100	104	PF00917	0.424
DOC_USP7_MATH_1	107	111	PF00917	0.378
DOC_USP7_MATH_1	236	240	PF00917	0.402
DOC_USP7_MATH_1	360	364	PF00917	0.426
DOC_USP7_MATH_1	53	57	PF00917	0.370
DOC_USP7_MATH_1	713	717	PF00917	0.603
DOC_USP7_MATH_1	732	736	PF00917	0.337
DOC_USP7_UBL2_3	15	19	PF12436	0.666
DOC_USP7_UBL2_3	478	482	PF12436	0.369
DOC_WW_Pin1_4	340	345	PF00397	0.597
DOC_WW_Pin1_4	376	381	PF00397	0.581
DOC_WW_Pin1_4	820	825	PF00397	0.413
DOC_WW_Pin1_4	869	874	PF00397	0.309
LIG_14-3-3_CanoR_1	322	328	PF00244	0.350
LIG_14-3-3_CanoR_1	390	400	PF00244	0.387
LIG_14-3-3_CanoR_1	573	579	PF00244	0.443
LIG_14-3-3_CanoR_1	73	80	PF00244	0.380
LIG_Actin_WH2_2	831	849	PF00022	0.509
LIG_Actin_WH2_2	86	104	PF00022	0.343
LIG_BIR_III_4	310	314	PF00653	0.455
LIG_BRCT_BRCA1_1	580	584	PF00533	0.534
LIG_Clathr_ClatBox_1	201	205	PF01394	0.324
LIG_CtBP_PxDLS_1	659	664	PF00389	0.455
LIG_EH_1	440	444	PF12763	0.514
LIG_FHA_1	172	178	PF00498	0.379
LIG_FHA_1	206	212	PF00498	0.330
LIG_FHA_1	393	399	PF00498	0.400
LIG_FHA_1	405	411	PF00498	0.416
LIG_FHA_1	506	512	PF00498	0.497
LIG_FHA_1	562	568	PF00498	0.455
LIG_FHA_1	573	579	PF00498	0.452
LIG_FHA_1	585	591	PF00498	0.415
LIG_FHA_1	634	640	PF00498	0.722
LIG_FHA_1	720	726	PF00498	0.529
LIG_FHA_1	758	764	PF00498	0.534
LIG_FHA_1	821	827	PF00498	0.449
LIG_FHA_1	852	858	PF00498	0.316
LIG_FHA_1	870	876	PF00498	0.309
LIG_FHA_1	877	883	PF00498	0.310
LIG_FHA_2	130	136	PF00498	0.363
LIG_FHA_2	139	145	PF00498	0.286
LIG_FHA_2	167	173	PF00498	0.400
LIG_FHA_2	194	200	PF00498	0.335
LIG_FHA_2	20	26	PF00498	0.690
LIG_FHA_2	242	248	PF00498	0.419
LIG_FHA_2	322	328	PF00498	0.359
LIG_FHA_2	333	339	PF00498	0.271
LIG_FHA_2	474	480	PF00498	0.353
LIG_FHA_2	557	563	PF00498	0.448
LIG_FHA_2	892	898	PF00498	0.403
LIG_LIR_Apic_2	748	754	PF02991	0.511
LIG_LIR_Gen_1	118	124	PF02991	0.309
LIG_LIR_Gen_1	151	160	PF02991	0.309
LIG_LIR_Gen_1	327	336	PF02991	0.330
LIG_LIR_Gen_1	595	601	PF02991	0.586
LIG_LIR_Gen_1	767	777	PF02991	0.520
LIG_LIR_Nem_3	151	155	PF02991	0.309
LIG_LIR_Nem_3	217	222	PF02991	0.405
LIG_LIR_Nem_3	244	249	PF02991	0.417
LIG_LIR_Nem_3	595	600	PF02991	0.575
LIG_LIR_Nem_3	814	820	PF02991	0.561
LIG_LIR_Nem_3	886	892	PF02991	0.369
LIG_LYPXL_S_1	93	97	PF13949	0.374
LIG_LYPXL_yS_3	94	97	PF13949	0.374
LIG_NRBOX	112	118	PF00104	0.455
LIG_NRBOX	67	73	PF00104	0.309
LIG_PCNA_yPIPBox_3	314	322	PF02747	0.309
LIG_PCNA_yPIPBox_3	351	362	PF02747	0.583
LIG_Pex14_1	148	152	PF04695	0.309
LIG_PTB_Apo_2	364	371	PF02174	0.393
LIG_PTB_Phospho_1	364	370	PF10480	0.483
LIG_REV1ctd_RIR_1	381	391	PF16727	0.610
LIG_SH2_CRK	219	223	PF00017	0.431
LIG_SH2_CRK	693	697	PF00017	0.396
LIG_SH2_CRK	751	755	PF00017	0.531
LIG_SH2_GRB2like	176	179	PF00017	0.265
LIG_SH2_NCK_1	504	508	PF00017	0.489
LIG_SH2_PTP2	889	892	PF00017	0.374
LIG_SH2_SRC	176	179	PF00017	0.265
LIG_SH2_SRC	471	474	PF00017	0.324
LIG_SH2_SRC	889	892	PF00017	0.330
LIG_SH2_STAP1	574	578	PF00017	0.405
LIG_SH2_STAT5	152	155	PF00017	0.309
LIG_SH2_STAT5	176	179	PF00017	0.265
LIG_SH2_STAT5	225	228	PF00017	0.321
LIG_SH2_STAT5	232	235	PF00017	0.309
LIG_SH2_STAT5	416	419	PF00017	0.427
LIG_SH2_STAT5	423	426	PF00017	0.389
LIG_SH2_STAT5	541	544	PF00017	0.419
LIG_SH2_STAT5	574	577	PF00017	0.422
LIG_SH2_STAT5	889	892	PF00017	0.374
LIG_SH3_3	436	442	PF00018	0.430
LIG_SH3_3	698	704	PF00018	0.491
LIG_SH3_3	7	13	PF00018	0.688
LIG_SH3_3	80	86	PF00018	0.324
LIG_SH3_3	845	851	PF00018	0.441
LIG_SH3_3	852	858	PF00018	0.324
LIG_SUMO_SIM_anti_2	196	203	PF11976	0.312
LIG_SUMO_SIM_anti_2	44	51	PF11976	0.344
LIG_SUMO_SIM_par_1	589	595	PF11976	0.564
LIG_SUMO_SIM_par_1	650	657	PF11976	0.408
LIG_SUMO_SIM_par_1	7	14	PF11976	0.723
LIG_SUMO_SIM_par_1	872	877	PF11976	0.328
LIG_TRAF2_1	324	327	PF00917	0.429
LIG_TRAF2_1	623	626	PF00917	0.805
LIG_TRAF2_1	631	634	PF00917	0.796
LIG_TYR_ITIM	539	544	PF00017	0.413
LIG_TYR_ITIM	691	696	PF00017	0.391
LIG_TYR_ITIM	887	892	PF00017	0.330
LIG_WRC_WIRS_1	116	121	PF05994	0.309
MOD_CDK_SPK_2	824	829	PF00069	0.435
MOD_CDK_SPxxK_3	340	347	PF00069	0.554
MOD_CK1_1	118	124	PF00069	0.449
MOD_CK1_1	419	425	PF00069	0.545
MOD_CK1_1	746	752	PF00069	0.557
MOD_CK1_1	76	82	PF00069	0.319
MOD_CK2_1	129	135	PF00069	0.455
MOD_CK2_1	166	172	PF00069	0.414
MOD_CK2_1	19	25	PF00069	0.687
MOD_CK2_1	234	240	PF00069	0.401
MOD_CK2_1	277	283	PF00069	0.436
MOD_CK2_1	321	327	PF00069	0.442
MOD_CK2_1	332	338	PF00069	0.439
MOD_CK2_1	340	346	PF00069	0.660
MOD_CK2_1	473	479	PF00069	0.353
MOD_CK2_1	510	516	PF00069	0.438
MOD_CK2_1	891	897	PF00069	0.369
MOD_GlcNHglycan	345	350	PF01048	0.686
MOD_GlcNHglycan	362	365	PF01048	0.317
MOD_GlcNHglycan	431	434	PF01048	0.512
MOD_GlcNHglycan	512	515	PF01048	0.457
MOD_GlcNHglycan	683	686	PF01048	0.466
MOD_GlcNHglycan	696	699	PF01048	0.399
MOD_GlcNHglycan	76	79	PF01048	0.380
MOD_GlcNHglycan	876	879	PF01048	0.354
MOD_GSK3_1	101	108	PF00069	0.343
MOD_GSK3_1	120	127	PF00069	0.438
MOD_GSK3_1	166	173	PF00069	0.426
MOD_GSK3_1	227	234	PF00069	0.324
MOD_GSK3_1	273	280	PF00069	0.368
MOD_GSK3_1	713	720	PF00069	0.480
MOD_GSK3_1	72	79	PF00069	0.431
MOD_GSK3_1	739	746	PF00069	0.582
MOD_GSK3_1	796	803	PF00069	0.562
MOD_GSK3_1	820	827	PF00069	0.417
MOD_GSK3_1	862	869	PF00069	0.309
MOD_N-GLC_1	621	626	PF02516	0.838
MOD_N-GLC_1	783	788	PF02516	0.463
MOD_N-GLC_1	789	794	PF02516	0.382
MOD_NEK2_1	101	106	PF00069	0.330
MOD_NEK2_1	124	129	PF00069	0.282
MOD_NEK2_1	138	143	PF00069	0.501
MOD_NEK2_1	315	320	PF00069	0.350
MOD_NEK2_1	48	53	PF00069	0.296
MOD_NEK2_1	561	566	PF00069	0.441
MOD_NEK2_1	584	589	PF00069	0.523
MOD_NEK2_1	660	665	PF00069	0.525
MOD_NEK2_1	672	677	PF00069	0.480
MOD_NEK2_1	71	76	PF00069	0.336
MOD_NEK2_1	777	782	PF00069	0.538
MOD_NEK2_1	800	805	PF00069	0.415
MOD_NEK2_1	807	812	PF00069	0.454
MOD_NEK2_2	241	246	PF00069	0.485
MOD_NEK2_2	505	510	PF00069	0.510
MOD_PIKK_1	101	107	PF00454	0.374
MOD_PIKK_1	315	321	PF00454	0.324
MOD_PIKK_1	866	872	PF00454	0.403
MOD_PKA_2	166	172	PF00069	0.299
MOD_PKA_2	234	240	PF00069	0.455
MOD_PKA_2	321	327	PF00069	0.375
MOD_PKA_2	456	462	PF00069	0.504
MOD_PKA_2	572	578	PF00069	0.445
MOD_PKA_2	72	78	PF00069	0.436
MOD_Plk_1	19	25	PF00069	0.797
MOD_Plk_1	193	199	PF00069	0.469
MOD_Plk_1	419	425	PF00069	0.509
MOD_Plk_1	789	795	PF00069	0.414
MOD_Plk_4	115	121	PF00069	0.309
MOD_Plk_4	419	425	PF00069	0.509
MOD_Plk_4	505	511	PF00069	0.427
MOD_Plk_4	518	524	PF00069	0.378
MOD_Plk_4	556	562	PF00069	0.492
MOD_Plk_4	592	598	PF00069	0.562
MOD_Plk_4	667	673	PF00069	0.572
MOD_Plk_4	746	752	PF00069	0.436
MOD_Plk_4	796	802	PF00069	0.452
MOD_Plk_4	807	813	PF00069	0.394
MOD_Plk_4	891	897	PF00069	0.417
MOD_ProDKin_1	340	346	PF00069	0.592
MOD_ProDKin_1	376	382	PF00069	0.577
MOD_ProDKin_1	820	826	PF00069	0.411
MOD_ProDKin_1	869	875	PF00069	0.309
MOD_SUMO_for_1	42	45	PF00179	0.636
MOD_SUMO_for_1	629	632	PF00179	0.721
MOD_SUMO_rev_2	273	280	PF00179	0.330
MOD_SUMO_rev_2	30	36	PF00179	0.727
MOD_SUMO_rev_2	343	348	PF00179	0.683
MOD_SUMO_rev_2	479	484	PF00179	0.381
MOD_SUMO_rev_2	491	497	PF00179	0.531
MOD_SUMO_rev_2	528	534	PF00179	0.541
MOD_SUMO_rev_2	632	637	PF00179	0.767
MOD_SUMO_rev_2	880	890	PF00179	0.471
TRG_DiLeu_BaEn_1	303	308	PF01217	0.343
TRG_DiLeu_BaEn_1	44	49	PF01217	0.578
TRG_DiLeu_BaEn_1	493	498	PF01217	0.591
TRG_DiLeu_BaEn_2	150	156	PF01217	0.309
TRG_DiLeu_BaEn_4	303	309	PF01217	0.324
TRG_DiLeu_BaLyEn_6	484	489	PF01217	0.455
TRG_ENDOCYTIC_2	152	155	PF00928	0.309
TRG_ENDOCYTIC_2	219	222	PF00928	0.433
TRG_ENDOCYTIC_2	249	252	PF00928	0.309
TRG_ENDOCYTIC_2	541	544	PF00928	0.393
TRG_ENDOCYTIC_2	574	577	PF00928	0.382
TRG_ENDOCYTIC_2	693	696	PF00928	0.391
TRG_ENDOCYTIC_2	889	892	PF00928	0.343
TRG_ENDOCYTIC_2	94	97	PF00928	0.324
TRG_ER_diArg_1	111	113	PF00400	0.403
TRG_NES_CRM1_1	115	125	PF08389	0.403
TRG_NES_CRM1_1	203	215	PF08389	0.374
TRG_Pf-PMV_PEXEL_1	111	115	PF00026	0.188
TRG_Pf-PMV_PEXEL_1	131	135	PF00026	0.188
TRG_Pf-PMV_PEXEL_1	268	273	PF00026	0.352
TRG_Pf-PMV_PEXEL_1	334	338	PF00026	0.443
TRG_Pf-PMV_PEXEL_1	91	96	PF00026	0.381

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCY7	Leptomonas seymouri	85%	100%
A0A0S4KJP7	Bodo saltans	50%	98%
A0A1X0NZE4	Trypanosomatidae	61%	100%
A0A3S7X246	Leishmania donovani	98%	100%
A0A422NUV0	Trypanosoma rangeli	60%	100%
A4HH20	Leishmania braziliensis	92%	100%
A4I449	Leishmania infantum	98%	100%
C9ZKR3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
E9AM67	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
P38764	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	32%	92%
P56701	Bos taurus	36%	100%
P87048	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	100%
Q13200	Homo sapiens	36%	100%
Q4FZT9	Rattus norvegicus	36%	100%
Q54BC6	Dictyostelium discoideum	35%	100%
Q5R9I6	Pongo abelii	36%	100%
Q6FPV6	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	33%	92%
Q6XJG8	Arabidopsis thaliana	36%	100%
Q7S8R8	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	34%	100%
Q8SS65	Encephalitozoon cuniculi (strain GB-M1)	23%	100%
Q8VDM4	Mus musculus	36%	100%
Q9SIV2	Arabidopsis thaliana	36%	100%
V5DLC9	Trypanosoma cruzi	60%	100%