Tryptophan--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tryptophanyl-tRNA synthetase, putative

Species:

Leishmania major

UniProt:

E9ADK8_LEIMA

TriTrypDb:

LmjF.29.0060 , LMJLV39_290005600 , LMJSD75_290005600

Length:

480

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ADK8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADK8

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006436	tryptophanyl-tRNA aminoacylation	7	12
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004830	tryptophan-tRNA ligase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	139	141	PF00675	0.289
CLV_NRD_NRD_1	451	453	PF00675	0.360
CLV_NRD_NRD_1	478	480	PF00675	0.604
CLV_PCSK_KEX2_1	139	141	PF00082	0.289
CLV_PCSK_KEX2_1	417	419	PF00082	0.218
CLV_PCSK_PC1ET2_1	417	419	PF00082	0.218
CLV_PCSK_SKI1_1	108	112	PF00082	0.388
CLV_PCSK_SKI1_1	140	144	PF00082	0.282
CLV_PCSK_SKI1_1	206	210	PF00082	0.261
CLV_PCSK_SKI1_1	280	284	PF00082	0.210
CLV_PCSK_SKI1_1	369	373	PF00082	0.205
CLV_PCSK_SKI1_1	377	381	PF00082	0.230
CLV_PCSK_SKI1_1	407	411	PF00082	0.308
CLV_PCSK_SKI1_1	427	431	PF00082	0.116
CLV_PCSK_SKI1_1	434	438	PF00082	0.198
CLV_PCSK_SKI1_1	439	443	PF00082	0.213
CLV_PCSK_SKI1_1	453	457	PF00082	0.368
DEG_SCF_FBW7_1	28	35	PF00400	0.331
DOC_AGCK_PIF_2	241	246	PF00069	0.403
DOC_CKS1_1	29	34	PF01111	0.333
DOC_CKS1_1	94	99	PF01111	0.373
DOC_CYCLIN_RxL_1	19	31	PF00134	0.329
DOC_CYCLIN_yCln2_LP_2	57	63	PF00134	0.317
DOC_CYCLIN_yCln2_LP_2	73	79	PF00134	0.321
DOC_MAPK_gen_1	387	393	PF00069	0.449
DOC_PP1_RVXF_1	138	145	PF00149	0.298
DOC_PP1_RVXF_1	21	28	PF00149	0.329
DOC_PP1_RVXF_1	234	240	PF00149	0.418
DOC_PP2B_LxvP_1	18	21	PF13499	0.318
DOC_PP4_FxxP_1	193	196	PF00568	0.403
DOC_USP7_MATH_1	288	292	PF00917	0.413
DOC_USP7_UBL2_3	206	210	PF12436	0.500
DOC_USP7_UBL2_3	417	421	PF12436	0.418
DOC_WW_Pin1_4	28	33	PF00397	0.329
DOC_WW_Pin1_4	364	369	PF00397	0.506
DOC_WW_Pin1_4	72	77	PF00397	0.344
DOC_WW_Pin1_4	93	98	PF00397	0.378
LIG_14-3-3_CanoR_1	168	177	PF00244	0.403
LIG_14-3-3_CanoR_1	41	47	PF00244	0.289
LIG_14-3-3_CanoR_1	452	462	PF00244	0.374
LIG_14-3-3_CanoR_1	48	57	PF00244	0.312
LIG_Actin_WH2_2	210	228	PF00022	0.506
LIG_Actin_WH2_2	320	337	PF00022	0.403
LIG_BRCT_BRCA1_1	292	296	PF00533	0.403
LIG_BRCT_BRCA1_1	340	344	PF00533	0.403
LIG_EVH1_1	5	9	PF00568	0.380
LIG_FHA_1	15	21	PF00498	0.354
LIG_FHA_1	184	190	PF00498	0.421
LIG_FHA_1	220	226	PF00498	0.403
LIG_FHA_1	322	328	PF00498	0.410
LIG_FHA_1	428	434	PF00498	0.413
LIG_FHA_1	440	446	PF00498	0.470
LIG_FHA_2	200	206	PF00498	0.404
LIG_FHA_2	211	217	PF00498	0.403
LIG_FHA_2	395	401	PF00498	0.440
LIG_FHA_2	454	460	PF00498	0.366
LIG_LIR_Apic_2	191	196	PF02991	0.403
LIG_LIR_Gen_1	103	113	PF02991	0.353
LIG_LIR_Gen_1	243	251	PF02991	0.427
LIG_LIR_Gen_1	45	50	PF02991	0.294
LIG_LIR_Gen_1	461	471	PF02991	0.471
LIG_LIR_Gen_1	81	90	PF02991	0.387
LIG_LIR_Nem_3	103	109	PF02991	0.356
LIG_LIR_Nem_3	238	244	PF02991	0.403
LIG_LIR_Nem_3	293	299	PF02991	0.431
LIG_LIR_Nem_3	3	8	PF02991	0.393
LIG_LIR_Nem_3	318	323	PF02991	0.430
LIG_LIR_Nem_3	341	347	PF02991	0.403
LIG_LIR_Nem_3	45	49	PF02991	0.299
LIG_LIR_Nem_3	461	466	PF02991	0.473
LIG_MLH1_MIPbox_1	340	344	PF16413	0.403
LIG_NRBOX	11	17	PF00104	0.338
LIG_Pex14_1	405	409	PF04695	0.403
LIG_Pex14_2	292	296	PF04695	0.403
LIG_REV1ctd_RIR_1	141	151	PF16727	0.267
LIG_REV1ctd_RIR_1	205	214	PF16727	0.445
LIG_RPA_C_Fungi	36	48	PF08784	0.299
LIG_SH2_CRK	106	110	PF00017	0.338
LIG_SH2_CRK	251	255	PF00017	0.237
LIG_SH2_STAP1	106	110	PF00017	0.328
LIG_SH2_STAT3	246	249	PF00017	0.285
LIG_SH2_STAT5	11	14	PF00017	0.335
LIG_SH2_STAT5	163	166	PF00017	0.249
LIG_SH2_STAT5	246	249	PF00017	0.331
LIG_SH2_STAT5	261	264	PF00017	0.331
LIG_SH2_STAT5	320	323	PF00017	0.259
LIG_SH2_STAT5	333	336	PF00017	0.237
LIG_SH2_STAT5	46	49	PF00017	0.311
LIG_SH3_3	1	7	PF00018	0.507
LIG_SH3_3	26	32	PF00018	0.336
LIG_SH3_3	396	402	PF00018	0.237
LIG_SH3_3	465	471	PF00018	0.345
LIG_SH3_3	91	97	PF00018	0.399
LIG_TRAF2_1	213	216	PF00917	0.352
LIG_TRAF2_1	447	450	PF00917	0.405
LIG_TRAF2_1	472	475	PF00917	0.385
LIG_TRAF2_1	89	92	PF00917	0.522
LIG_TYR_ITIM	104	109	PF00017	0.348
LIG_UBA3_1	180	187	PF00899	0.252
LIG_UBA3_1	299	304	PF00899	0.300
LIG_WW_2	6	9	PF00397	0.348
MOD_CDK_SPK_2	364	369	PF00069	0.382
MOD_CDK_SPxK_1	28	34	PF00069	0.332
MOD_CK1_1	171	177	PF00069	0.247
MOD_CK1_1	355	361	PF00069	0.252
MOD_CK1_1	443	449	PF00069	0.317
MOD_CK1_1	72	78	PF00069	0.323
MOD_CK2_1	199	205	PF00069	0.243
MOD_CK2_1	210	216	PF00069	0.237
MOD_Cter_Amidation	130	133	PF01082	0.267
MOD_GlcNHglycan	12	15	PF01048	0.397
MOD_GlcNHglycan	292	295	PF01048	0.298
MOD_GlcNHglycan	347	350	PF01048	0.270
MOD_GlcNHglycan	380	383	PF01048	0.237
MOD_GSK3_1	10	17	PF00069	0.338
MOD_GSK3_1	28	35	PF00069	0.331
MOD_GSK3_1	439	446	PF00069	0.385
MOD_GSK3_1	68	75	PF00069	0.309
MOD_NEK2_1	183	188	PF00069	0.270
MOD_NEK2_1	189	194	PF00069	0.275
MOD_NEK2_1	347	352	PF00069	0.273
MOD_NEK2_1	354	359	PF00069	0.284
MOD_NEK2_1	69	74	PF00069	0.309
MOD_NEK2_2	14	19	PF00069	0.336
MOD_PIKK_1	261	267	PF00454	0.237
MOD_PIKK_1	48	54	PF00454	0.329
MOD_PKA_1	453	459	PF00069	0.440
MOD_PKA_2	127	133	PF00069	0.472
MOD_PKA_2	321	327	PF00069	0.244
MOD_Plk_1	210	216	PF00069	0.292
MOD_Plk_4	235	241	PF00069	0.237
MOD_Plk_4	42	48	PF00069	0.309
MOD_Plk_4	440	446	PF00069	0.236
MOD_ProDKin_1	28	34	PF00069	0.332
MOD_ProDKin_1	364	370	PF00069	0.382
MOD_ProDKin_1	72	78	PF00069	0.342
MOD_ProDKin_1	93	99	PF00069	0.379
MOD_SUMO_for_1	272	275	PF00179	0.250
MOD_SUMO_for_1	472	475	PF00179	0.385
MOD_SUMO_rev_2	203	208	PF00179	0.237
MOD_SUMO_rev_2	411	419	PF00179	0.375
TRG_DiLeu_BaEn_4	414	420	PF01217	0.382
TRG_DiLeu_BaLyEn_6	45	50	PF01217	0.294
TRG_ENDOCYTIC_2	106	109	PF00928	0.395
TRG_ENDOCYTIC_2	251	254	PF00928	0.237
TRG_ENDOCYTIC_2	320	323	PF00928	0.237
TRG_ENDOCYTIC_2	46	49	PF00928	0.298
TRG_ER_diArg_1	138	140	PF00400	0.278
TRG_NLS_Bipartite_1	434	456	PF00514	0.444
TRG_NLS_MonoExtC_3	451	457	PF00514	0.438
TRG_Pf-PMV_PEXEL_1	48	52	PF00026	0.306

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3G3	Leptomonas seymouri	85%	100%
A0A0N1HXL2	Leptomonas seymouri	41%	100%
A0A0S4IN16	Bodo saltans	76%	100%
A0A0S4JC70	Bodo saltans	43%	100%
A0A1X0NGJ6	Trypanosomatidae	40%	100%
A0A1X0NZB4	Trypanosomatidae	78%	100%
A0A3R7MPN7	Trypanosoma rangeli	79%	100%
A0A3S7WXL2	Leishmania donovani	45%	100%
A0A3S7X238	Leishmania donovani	97%	100%
A0A422NW64	Trypanosoma rangeli	41%	100%
A2BLD4	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	30%	100%
A3MX72	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	38%	100%
A4HCP4	Leishmania braziliensis	39%	98%
A4HH14	Leishmania braziliensis	88%	100%
A4I070	Leishmania infantum	45%	100%
A4I443	Leishmania infantum	97%	100%
A4WL99	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	36%	100%
B6YUH1	Thermococcus onnurineus (strain NA1)	46%	100%
C6A032	Thermococcus sibiricus (strain DSM 12597 / MM 739)	46%	100%
C9ZKR9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	79%	100%
E9AM73	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
E9AW33	Leishmania mexicana (strain MHOM/GT/2001/U1103)	45%	100%
O26352	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	29%	100%
O59584	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	46%	100%
O96771	Encephalitozoon cuniculi (strain GB-M1)	43%	100%
P17248	Bos taurus	56%	100%
P23381	Homo sapiens	56%	100%
P23612	Oryctolagus cuniculus	56%	100%
P32921	Mus musculus	57%	100%
Q09692	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	55%	100%
Q12109	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	51%	100%
Q4JBG7	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	47%	100%
Q4QBE4	Leishmania major	44%	100%
Q55DZ8	Dictyostelium discoideum	53%	100%
Q58810	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	30%	100%
Q5JEP3	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	46%	100%
Q5R4J1	Pongo abelii	56%	100%
Q6P7B0	Rattus norvegicus	57%	100%
Q8TYF7	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	30%	100%
Q8U453	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	47%	100%
Q8ZTU5	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	36%	100%
Q976M1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	47%	100%
Q97ZX0	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	47%	100%
Q9HN66	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	35%	100%
Q9SR15	Arabidopsis thaliana	55%	100%
Q9UY11	Pyrococcus abyssi (strain GE5 / Orsay)	45%	100%
Q9Y924	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	31%	100%
V5B4S5	Trypanosoma cruzi	80%	100%