MSP domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

MSP (Major sperm protein) domain containing protein, putative

Species:

Leishmania major

UniProt:

E9ADJ2_LEIMA

TriTrypDb:

LmjF.27.2120 , LMJLV39_270028700 , LMJSD75_270028600

Length:

337

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	2
GO:0005886	plasma membrane	3	2
GO:0016020	membrane	2	5
GO:0020016	ciliary pocket	2	2
GO:0031090	organelle membrane	3	2
GO:0110165	cellular anatomical entity	1	5

Expansion

Sequence features

E9ADJ2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADJ2

Function

Biological processes
Term	Name	Level	Count
GO:0006605	protein targeting	5	6
GO:0006612	protein targeting to membrane	5	6
GO:0006613	cotranslational protein targeting to membrane	6	6
GO:0006614	SRP-dependent cotranslational protein targeting to membrane	7	6
GO:0006810	transport	3	6
GO:0006886	intracellular protein transport	4	6
GO:0008104	protein localization	4	6
GO:0009987	cellular process	1	6
GO:0015031	protein transport	4	6
GO:0016043	cellular component organization	3	2
GO:0022406	membrane docking	2	2
GO:0033036	macromolecule localization	2	6
GO:0033365	protein localization to organelle	5	6
GO:0045047	protein targeting to ER	6	6
GO:0045184	establishment of protein localization	3	6
GO:0046907	intracellular transport	3	6
GO:0051179	localization	1	6
GO:0051234	establishment of localization	2	6
GO:0051640	organelle localization	2	2
GO:0051641	cellular localization	2	6
GO:0051643	endoplasmic reticulum localization	3	2
GO:0051649	establishment of localization in cell	3	6
GO:0051668	localization within membrane	3	6
GO:0061024	membrane organization	4	2
GO:0061817	endoplasmic reticulum-plasma membrane tethering	4	2
GO:0070727	cellular macromolecule localization	3	6
GO:0070972	protein localization to endoplasmic reticulum	6	6
GO:0071702	organic substance transport	4	6
GO:0071705	nitrogen compound transport	4	6
GO:0071840	cellular component organization or biogenesis	2	2
GO:0072594	establishment of protein localization to organelle	4	6
GO:0072599	establishment of protein localization to endoplasmic reticulum	5	6
GO:0072657	protein localization to membrane	4	6
GO:0090150	establishment of protein localization to membrane	4	6
GO:0090158	endoplasmic reticulum membrane organization	5	2
GO:0140056	organelle localization by membrane tethering	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	6
GO:0005488	binding	1	6
GO:0005525	GTP binding	5	6
GO:0017076	purine nucleotide binding	4	6
GO:0019001	guanyl nucleotide binding	5	6
GO:0032553	ribonucleotide binding	3	6
GO:0032555	purine ribonucleotide binding	4	6
GO:0032561	guanyl ribonucleotide binding	5	6
GO:0035639	purine ribonucleoside triphosphate binding	4	6
GO:0036094	small molecule binding	2	6
GO:0043167	ion binding	2	6
GO:0043168	anion binding	3	6
GO:0097159	organic cyclic compound binding	2	6
GO:0097367	carbohydrate derivative binding	2	6
GO:1901265	nucleoside phosphate binding	3	6
GO:1901363	heterocyclic compound binding	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_SKI1_1	165	169	PF00082	0.399
CLV_PCSK_SKI1_1	190	194	PF00082	0.363
CLV_PCSK_SKI1_1	33	37	PF00082	0.341
CLV_PCSK_SKI1_1	87	91	PF00082	0.381
DEG_APCC_DBOX_1	160	168	PF00400	0.529
DEG_APCC_KENBOX_2	152	156	PF00400	0.509
DEG_SPOP_SBC_1	205	209	PF00917	0.765
DOC_CYCLIN_yClb5_NLxxxL_5	324	333	PF00134	0.447
DOC_CYCLIN_yCln2_LP_2	15	21	PF00134	0.507
DOC_MAPK_HePTP_8	56	68	PF00069	0.553
DOC_MAPK_MEF2A_6	103	110	PF00069	0.499
DOC_MAPK_MEF2A_6	309	317	PF00069	0.360
DOC_MAPK_MEF2A_6	59	68	PF00069	0.537
DOC_PP4_FxxP_1	17	20	PF00568	0.494
DOC_USP7_MATH_1	140	144	PF00917	0.576
DOC_USP7_MATH_1	205	209	PF00917	0.792
DOC_USP7_MATH_1	263	267	PF00917	0.761
DOC_WW_Pin1_4	153	158	PF00397	0.614
DOC_WW_Pin1_4	229	234	PF00397	0.726
DOC_WW_Pin1_4	242	247	PF00397	0.618
DOC_WW_Pin1_4	268	273	PF00397	0.798
LIG_14-3-3_CanoR_1	203	212	PF00244	0.698
LIG_14-3-3_CanoR_1	236	246	PF00244	0.759
LIG_14-3-3_CanoR_1	59	63	PF00244	0.603
LIG_APCC_ABBAyCdc20_2	103	109	PF00400	0.543
LIG_BRCT_BRCA1_1	13	17	PF00533	0.507
LIG_EVH1_2	251	255	PF00568	0.626
LIG_EVH1_2	64	68	PF00568	0.579
LIG_FHA_1	3	9	PF00498	0.622
LIG_FHA_1	329	335	PF00498	0.423
LIG_FHA_2	286	292	PF00498	0.650
LIG_LIR_Apic_2	14	20	PF02991	0.534
LIG_LIR_Gen_1	109	118	PF02991	0.534
LIG_LIR_Gen_1	135	142	PF02991	0.470
LIG_LIR_Nem_3	109	113	PF02991	0.523
LIG_LIR_Nem_3	13	19	PF02991	0.505
LIG_LIR_Nem_3	135	139	PF02991	0.492
LIG_LIR_Nem_3	307	311	PF02991	0.516
LIG_LIR_Nem_3	49	53	PF02991	0.573
LIG_MLH1_MIPbox_1	13	17	PF16413	0.507
LIG_NRBOX	319	325	PF00104	0.358
LIG_PCNA_yPIPBox_3	119	133	PF02747	0.591
LIG_PCNA_yPIPBox_3	23	33	PF02747	0.549
LIG_PCNA_yPIPBox_3	326	336	PF02747	0.416
LIG_Pex14_2	17	21	PF04695	0.577
LIG_SH2_CRK	308	312	PF00017	0.573
LIG_SH3_3	110	116	PF00018	0.609
LIG_SH3_3	215	221	PF00018	0.668
LIG_SH3_3	59	65	PF00018	0.596
LIG_TYR_ITIM	306	311	PF00017	0.599
LIG_UBA3_1	329	336	PF00899	0.424
LIG_WRC_WIRS_1	47	52	PF05994	0.564
LIG_WRC_WIRS_1	78	83	PF05994	0.573
MOD_CDK_SPK_2	234	239	PF00069	0.659
MOD_CDK_SPxxK_3	229	236	PF00069	0.802
MOD_CK1_1	11	17	PF00069	0.500
MOD_CK1_1	117	123	PF00069	0.620
MOD_CK1_1	129	135	PF00069	0.659
MOD_CK1_1	156	162	PF00069	0.561
MOD_CK1_1	228	234	PF00069	0.707
MOD_CK1_1	237	243	PF00069	0.631
MOD_CK1_1	266	272	PF00069	0.706
MOD_CK1_1	275	281	PF00069	0.703
MOD_CK2_1	293	299	PF00069	0.667
MOD_GlcNHglycan	265	268	PF01048	0.533
MOD_GlcNHglycan	274	277	PF01048	0.493
MOD_GlcNHglycan	283	286	PF01048	0.401
MOD_GSK3_1	225	232	PF00069	0.725
MOD_GSK3_1	268	275	PF00069	0.808
MOD_GSK3_1	281	288	PF00069	0.602
MOD_GSK3_1	324	331	PF00069	0.516
MOD_GSK3_1	73	80	PF00069	0.578
MOD_N-GLC_1	2	7	PF02516	0.323
MOD_N-GLC_1	22	27	PF02516	0.358
MOD_NEK2_1	206	211	PF00069	0.668
MOD_NEK2_1	315	320	PF00069	0.348
MOD_NEK2_1	324	329	PF00069	0.553
MOD_NEK2_2	290	295	PF00069	0.659
MOD_PIKK_1	207	213	PF00454	0.768
MOD_PIKK_1	225	231	PF00454	0.724
MOD_PIKK_1	275	281	PF00454	0.722
MOD_PIKK_1	8	14	PF00454	0.505
MOD_PKA_2	223	229	PF00069	0.727
MOD_PKA_2	58	64	PF00069	0.559
MOD_Plk_1	140	146	PF00069	0.575
MOD_Plk_1	2	8	PF00069	0.512
MOD_Plk_1	22	28	PF00069	0.512
MOD_Plk_1	290	296	PF00069	0.655
MOD_Plk_4	11	17	PF00069	0.551
MOD_Plk_4	174	180	PF00069	0.557
MOD_Plk_4	315	321	PF00069	0.302
MOD_Plk_4	328	334	PF00069	0.370
MOD_Plk_4	73	79	PF00069	0.550
MOD_ProDKin_1	153	159	PF00069	0.605
MOD_ProDKin_1	229	235	PF00069	0.724
MOD_ProDKin_1	242	248	PF00069	0.616
MOD_ProDKin_1	268	274	PF00069	0.801
MOD_SUMO_for_1	182	185	PF00179	0.620
MOD_SUMO_rev_2	129	135	PF00179	0.594
TRG_ENDOCYTIC_2	308	311	PF00928	0.573
TRG_ER_diArg_1	200	203	PF00400	0.779
TRG_Pf-PMV_PEXEL_1	95	100	PF00026	0.438

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PF53	Leptomonas seymouri	67%	100%
A0A3Q8IEI8	Leishmania donovani	95%	100%
A0A422N827	Trypanosoma rangeli	29%	96%
A4HFY9	Leishmania braziliensis	74%	100%
A4I2Y2	Leishmania infantum	94%	100%
C9ZJH3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9AZB8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
V5AYR4	Trypanosoma cruzi	30%	96%