Putative endo/exonuclease Mre11

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

endo/exonuclease Mre11, putative

Species:

Leishmania major

UniProt:

E9ADG7_LEIMA

TriTrypDb:

LmjF.27.1890 , LMJLV39_270026000 , LMJSD75_270026000

Length:

853

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0030870	Mre11 complex	3	3
GO:0032991	protein-containing complex	1	3
GO:0035861	site of double-strand break	3	2
GO:0090734	site of DNA damage	2	2
GO:0110165	cellular anatomical entity	1	6
GO:0140513	nuclear protein-containing complex	2	3
GO:0005634	nucleus	5	4
GO:0043226	organelle	2	4
GO:0043227	membrane-bounded organelle	3	4
GO:0043229	intracellular organelle	3	4
GO:0043231	intracellular membrane-bounded organelle	4	4

Expansion

Sequence features

E9ADG7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADG7

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	2
GO:0000077	DNA damage checkpoint signaling	5	2
GO:0000723	telomere maintenance	5	2
GO:0000724	double-strand break repair via homologous recombination	7	2
GO:0000725	recombinational repair	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006259	DNA metabolic process	4	7
GO:0006281	DNA repair	5	7
GO:0006302	double-strand break repair	6	7
GO:0006303	double-strand break repair via nonhomologous end joining	7	2
GO:0006310	DNA recombination	5	2
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0006950	response to stress	2	7
GO:0006974	DNA damage response	4	7
GO:0006996	organelle organization	4	2
GO:0007093	mitotic cell cycle checkpoint signaling	4	2
GO:0007095	mitotic G2 DNA damage checkpoint signaling	6	2
GO:0007165	signal transduction	2	2
GO:0007346	regulation of mitotic cell cycle	5	2
GO:0008152	metabolic process	1	7
GO:0009987	cellular process	1	7
GO:0010389	regulation of G2/M transition of mitotic cell cycle	7	2
GO:0010564	regulation of cell cycle process	5	2
GO:0010948	negative regulation of cell cycle process	6	2
GO:0010972	negative regulation of G2/M transition of mitotic cell cycle	8	2
GO:0016043	cellular component organization	3	2
GO:0022402	cell cycle process	2	2
GO:0022414	reproductive process	1	3
GO:0031570	DNA integrity checkpoint signaling	5	2
GO:0032200	telomere organization	6	2
GO:0033554	cellular response to stress	3	7
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0035556	intracellular signal transduction	3	2
GO:0042138	meiotic DNA double-strand break formation	4	2
GO:0042770	signal transduction in response to DNA damage	4	2
GO:0043170	macromolecule metabolic process	3	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0044260	obsolete cellular macromolecule metabolic process	3	7
GO:0044773	mitotic DNA damage checkpoint signaling	6	2
GO:0044774	mitotic DNA integrity checkpoint signaling	5	2
GO:0044818	mitotic G2/M transition checkpoint	5	2
GO:0045786	negative regulation of cell cycle	5	2
GO:0045930	negative regulation of mitotic cell cycle	6	2
GO:0046483	heterocycle metabolic process	3	7
GO:0048519	negative regulation of biological process	3	2
GO:0048523	negative regulation of cellular process	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050896	response to stimulus	1	7
GO:0051276	chromosome organization	5	2
GO:0051716	cellular response to stimulus	2	7
GO:0051726	regulation of cell cycle	4	2
GO:0061982	meiosis I cell cycle process	3	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	7
GO:0071840	cellular component organization or biogenesis	2	2
GO:0090304	nucleic acid metabolic process	4	7
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	2
GO:0097551	mitochondrial double-strand break repair	7	2
GO:0097552	mitochondrial double-strand break repair via homologous recombination	7	2
GO:1901360	organic cyclic compound metabolic process	3	7
GO:1901987	regulation of cell cycle phase transition	6	2
GO:1901988	negative regulation of cell cycle phase transition	7	2
GO:1901990	regulation of mitotic cell cycle phase transition	6	2
GO:1901991	negative regulation of mitotic cell cycle phase transition	7	2
GO:1902749	regulation of cell cycle G2/M phase transition	7	2
GO:1902750	negative regulation of cell cycle G2/M phase transition	8	2
GO:1903046	meiotic cell cycle process	2	2
GO:1903047	mitotic cell cycle process	3	2
GO:0007049	cell cycle	2	1
GO:0051321	meiotic cell cycle	2	1

Molecular functions
Term	Name	Level	Count
GO:0000014	single-stranded DNA endodeoxyribonuclease activity	6	2
GO:0003824	catalytic activity	1	7
GO:0004518	nuclease activity	4	7
GO:0004519	endonuclease activity	5	7
GO:0004520	DNA endonuclease activity	5	3
GO:0004527	exonuclease activity	5	7
GO:0004536	DNA nuclease activity	4	3
GO:0005488	binding	1	7
GO:0016787	hydrolase activity	2	7
GO:0016788	hydrolase activity, acting on ester bonds	3	7
GO:0030145	manganese ion binding	6	7
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7
GO:0046914	transition metal ion binding	5	7
GO:0140097	catalytic activity, acting on DNA	3	3
GO:0140640	catalytic activity, acting on a nucleic acid	2	3
GO:0004529	DNA exonuclease activity	5	1
GO:0008296	3'-5'-DNA exonuclease activity	7	1
GO:0008408	3'-5' exonuclease activity	6	1
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	1
GO:0016895	DNA exonuclease activity, producing 5'-phosphomonoesters	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	311	315	PF00656	0.475
CLV_C14_Caspase3-7	800	804	PF00656	0.581
CLV_NRD_NRD_1	161	163	PF00675	0.312
CLV_NRD_NRD_1	24	26	PF00675	0.352
CLV_NRD_NRD_1	303	305	PF00675	0.275
CLV_NRD_NRD_1	405	407	PF00675	0.295
CLV_NRD_NRD_1	496	498	PF00675	0.469
CLV_NRD_NRD_1	573	575	PF00675	0.604
CLV_NRD_NRD_1	641	643	PF00675	0.637
CLV_NRD_NRD_1	73	75	PF00675	0.312
CLV_NRD_NRD_1	743	745	PF00675	0.603
CLV_NRD_NRD_1	771	773	PF00675	0.843
CLV_PCSK_FUR_1	639	643	PF00082	0.636
CLV_PCSK_KEX2_1	24	26	PF00082	0.352
CLV_PCSK_KEX2_1	303	305	PF00082	0.275
CLV_PCSK_KEX2_1	405	407	PF00082	0.295
CLV_PCSK_KEX2_1	496	498	PF00082	0.469
CLV_PCSK_KEX2_1	520	522	PF00082	0.477
CLV_PCSK_KEX2_1	572	574	PF00082	0.616
CLV_PCSK_KEX2_1	641	643	PF00082	0.637
CLV_PCSK_KEX2_1	690	692	PF00082	0.618
CLV_PCSK_KEX2_1	743	745	PF00082	0.563
CLV_PCSK_KEX2_1	771	773	PF00082	0.843
CLV_PCSK_PC1ET2_1	520	522	PF00082	0.477
CLV_PCSK_PC1ET2_1	690	692	PF00082	0.618
CLV_PCSK_PC7_1	299	305	PF00082	0.275
CLV_PCSK_SKI1_1	397	401	PF00082	0.275
CLV_PCSK_SKI1_1	7	11	PF00082	0.312
CLV_PCSK_SKI1_1	75	79	PF00082	0.342
CLV_PCSK_SKI1_1	825	829	PF00082	0.658
CLV_Separin_Metazoa	35	39	PF03568	0.312
CLV_Separin_Metazoa	394	398	PF03568	0.475
CLV_Separin_Metazoa	580	584	PF03568	0.606
DEG_APCC_KENBOX_2	544	548	PF00400	0.516
DOC_CYCLIN_RxL_1	4	13	PF00134	0.312
DOC_CYCLIN_yCln2_LP_2	395	401	PF00134	0.529
DOC_CYCLIN_yCln2_LP_2	426	432	PF00134	0.512
DOC_MAPK_FxFP_2	192	195	PF00069	0.291
DOC_MAPK_gen_1	162	170	PF00069	0.312
DOC_MAPK_gen_1	183	192	PF00069	0.339
DOC_MAPK_gen_1	639	648	PF00069	0.545
DOC_MAPK_MEF2A_6	355	363	PF00069	0.475
DOC_PP1_RVXF_1	186	193	PF00149	0.291
DOC_PP1_SILK_1	504	509	PF00149	0.597
DOC_PP2B_LxvP_1	246	249	PF13499	0.397
DOC_PP2B_LxvP_1	426	429	PF13499	0.527
DOC_PP4_FxxP_1	105	108	PF00568	0.312
DOC_PP4_FxxP_1	192	195	PF00568	0.291
DOC_USP7_MATH_1	502	506	PF00917	0.518
DOC_USP7_MATH_1	563	567	PF00917	0.759
DOC_USP7_MATH_1	673	677	PF00917	0.713
DOC_USP7_MATH_1	829	833	PF00917	0.607
DOC_WW_Pin1_4	288	293	PF00397	0.503
DOC_WW_Pin1_4	561	566	PF00397	0.732
DOC_WW_Pin1_4	608	613	PF00397	0.634
LIG_14-3-3_CanoR_1	162	168	PF00244	0.312
LIG_14-3-3_CanoR_1	284	289	PF00244	0.409
LIG_14-3-3_CanoR_1	303	307	PF00244	0.475
LIG_14-3-3_CanoR_1	38	46	PF00244	0.312
LIG_14-3-3_CanoR_1	68	73	PF00244	0.312
LIG_14-3-3_CanoR_1	691	700	PF00244	0.664
LIG_Actin_WH2_2	147	164	PF00022	0.388
LIG_Actin_WH2_2	382	399	PF00022	0.475
LIG_BIR_II_1	1	5	PF00653	0.577
LIG_BIR_III_2	803	807	PF00653	0.580
LIG_BRCT_BRCA1_1	643	647	PF00533	0.546
LIG_BRCT_BRCA1_2	643	649	PF00533	0.550
LIG_EH1_1	43	51	PF00400	0.312
LIG_FHA_1	128	134	PF00498	0.312
LIG_FHA_1	3	9	PF00498	0.291
LIG_FHA_1	303	309	PF00498	0.542
LIG_FHA_1	434	440	PF00498	0.486
LIG_FHA_1	67	73	PF00498	0.326
LIG_FHA_1	722	728	PF00498	0.828
LIG_FHA_1	95	101	PF00498	0.402
LIG_FHA_2	147	153	PF00498	0.312
LIG_FHA_2	29	35	PF00498	0.312
LIG_FHA_2	40	46	PF00498	0.312
LIG_FHA_2	798	804	PF00498	0.583
LIG_Integrin_RGD_1	25	27	PF01839	0.312
LIG_LIR_Apic_2	370	376	PF02991	0.475
LIG_LIR_Gen_1	165	173	PF02991	0.316
LIG_LIR_Gen_1	253	260	PF02991	0.401
LIG_LIR_Gen_1	27	37	PF02991	0.312
LIG_LIR_Gen_1	364	373	PF02991	0.475
LIG_LIR_Gen_1	577	587	PF02991	0.604
LIG_LIR_Nem_3	138	144	PF02991	0.338
LIG_LIR_Nem_3	165	170	PF02991	0.316
LIG_LIR_Nem_3	253	258	PF02991	0.412
LIG_LIR_Nem_3	27	33	PF02991	0.312
LIG_LIR_Nem_3	3	9	PF02991	0.348
LIG_LIR_Nem_3	364	368	PF02991	0.475
LIG_LIR_Nem_3	493	498	PF02991	0.456
LIG_LIR_Nem_3	71	76	PF02991	0.350
LIG_LYPXL_yS_3	356	359	PF13949	0.475
LIG_NRBOX	482	488	PF00104	0.454
LIG_PALB2_WD40_1	199	207	PF16756	0.312
LIG_PCNA_yPIPBox_3	216	229	PF02747	0.291
LIG_Pex14_1	849	853	PF04695	0.511
LIG_Pex14_2	604	608	PF04695	0.717
LIG_SH2_GRB2like	141	144	PF00017	0.312
LIG_SH2_NCK_1	527	531	PF00017	0.557
LIG_SH2_STAP1	527	531	PF00017	0.505
LIG_SH2_STAT5	141	144	PF00017	0.312
LIG_SH2_STAT5	293	296	PF00017	0.475
LIG_SH2_STAT5	76	79	PF00017	0.312
LIG_SH3_3	109	115	PF00018	0.505
LIG_SH3_3	245	251	PF00018	0.405
LIG_SH3_3	254	260	PF00018	0.386
LIG_SH3_3	386	392	PF00018	0.475
LIG_SH3_3	395	401	PF00018	0.475
LIG_SH3_3	426	432	PF00018	0.466
LIG_SH3_3	581	587	PF00018	0.748
LIG_SH3_3	630	636	PF00018	0.634
LIG_SH3_3	682	688	PF00018	0.780
LIG_SH3_3	816	822	PF00018	0.698
LIG_SUMO_SIM_anti_2	130	138	PF11976	0.312
LIG_TRAF2_1	269	272	PF00917	0.425
LIG_TRAF2_1	32	35	PF00917	0.312
LIG_TRFH_1	288	292	PF08558	0.475
LIG_TYR_ITIM	354	359	PF00017	0.475
LIG_UBA3_1	483	492	PF00899	0.451
LIG_WRC_WIRS_1	30	35	PF05994	0.312
LIG_WRC_WIRS_1	321	326	PF05994	0.475
MOD_CDK_SPxxK_3	608	615	PF00069	0.532
MOD_CK1_1	2	8	PF00069	0.474
MOD_CK1_1	29	35	PF00069	0.312
MOD_CK1_1	327	333	PF00069	0.478
MOD_CK1_1	490	496	PF00069	0.471
MOD_CK1_1	847	853	PF00069	0.579
MOD_CK1_1	86	92	PF00069	0.312
MOD_CK2_1	146	152	PF00069	0.291
MOD_CK2_1	266	272	PF00069	0.419
MOD_CK2_1	28	34	PF00069	0.312
MOD_CK2_1	331	337	PF00069	0.529
MOD_CK2_1	456	462	PF00069	0.493
MOD_CK2_1	595	601	PF00069	0.679
MOD_GlcNHglycan	268	271	PF01048	0.506
MOD_GlcNHglycan	369	372	PF01048	0.275
MOD_GlcNHglycan	423	426	PF01048	0.288
MOD_GlcNHglycan	462	466	PF01048	0.295
MOD_GlcNHglycan	595	598	PF01048	0.740
MOD_GlcNHglycan	643	646	PF01048	0.653
MOD_GlcNHglycan	693	696	PF01048	0.647
MOD_GlcNHglycan	768	771	PF01048	0.669
MOD_GlcNHglycan	777	780	PF01048	0.594
MOD_GlcNHglycan	786	789	PF01048	0.518
MOD_GlcNHglycan	797	800	PF01048	0.606
MOD_GlcNHglycan	816	819	PF01048	0.541
MOD_GlcNHglycan	831	834	PF01048	0.776
MOD_GSK3_1	259	266	PF00069	0.405
MOD_GSK3_1	284	291	PF00069	0.399
MOD_GSK3_1	320	327	PF00069	0.511
MOD_GSK3_1	363	370	PF00069	0.475
MOD_GSK3_1	449	456	PF00069	0.447
MOD_GSK3_1	486	493	PF00069	0.452
MOD_GSK3_1	589	596	PF00069	0.664
MOD_GSK3_1	825	832	PF00069	0.656
MOD_GSK3_1	86	93	PF00069	0.371
MOD_N-GLC_1	449	454	PF02516	0.247
MOD_N-GLC_1	502	507	PF02516	0.600
MOD_N-GLC_2	453	455	PF02516	0.416
MOD_NEK2_1	1	6	PF00069	0.414
MOD_NEK2_1	135	140	PF00069	0.352
MOD_NEK2_1	324	329	PF00069	0.478
MOD_NEK2_1	486	491	PF00069	0.439
MOD_NEK2_1	591	596	PF00069	0.689
MOD_NEK2_1	66	71	PF00069	0.243
MOD_OFUCOSY	65	70	PF10250	0.243
MOD_PIKK_1	487	493	PF00454	0.445
MOD_PKA_1	162	168	PF00069	0.388
MOD_PKA_1	641	647	PF00069	0.545
MOD_PKA_2	283	289	PF00069	0.418
MOD_PKA_2	302	308	PF00069	0.592
MOD_PKA_2	641	647	PF00069	0.604
MOD_PKA_2	765	771	PF00069	0.636
MOD_PKB_1	639	647	PF00069	0.543
MOD_Plk_1	2	8	PF00069	0.398
MOD_Plk_1	26	32	PF00069	0.321
MOD_Plk_1	502	508	PF00069	0.599
MOD_Plk_1	720	726	PF00069	0.654
MOD_Plk_2-3	314	320	PF00069	0.475
MOD_Plk_2-3	331	337	PF00069	0.475
MOD_Plk_2-3	658	664	PF00069	0.677
MOD_Plk_4	163	169	PF00069	0.317
MOD_Plk_4	260	266	PF00069	0.371
MOD_Plk_4	320	326	PF00069	0.475
MOD_Plk_4	453	459	PF00069	0.475
MOD_Plk_4	490	496	PF00069	0.456
MOD_Plk_4	502	508	PF00069	0.562
MOD_Plk_4	673	679	PF00069	0.606
MOD_Plk_4	68	74	PF00069	0.312
MOD_Plk_4	806	812	PF00069	0.641
MOD_ProDKin_1	288	294	PF00069	0.503
MOD_ProDKin_1	561	567	PF00069	0.732
MOD_ProDKin_1	608	614	PF00069	0.629
MOD_SUMO_for_1	401	404	PF00179	0.529
MOD_SUMO_rev_2	54	62	PF00179	0.312
TRG_DiLeu_BaEn_1	242	247	PF01217	0.373
TRG_DiLeu_BaLyEn_6	354	359	PF01217	0.529
TRG_ENDOCYTIC_2	278	281	PF00928	0.355
TRG_ENDOCYTIC_2	356	359	PF00928	0.475
TRG_ENDOCYTIC_2	385	388	PF00928	0.475
TRG_ER_diArg_1	23	25	PF00400	0.312
TRG_ER_diArg_1	302	304	PF00400	0.475
TRG_ER_diArg_1	405	407	PF00400	0.549
TRG_ER_diArg_1	495	497	PF00400	0.494
TRG_ER_diArg_1	572	574	PF00400	0.627
TRG_ER_diArg_1	639	642	PF00400	0.717
TRG_ER_diArg_1	743	746	PF00400	0.565
TRG_NES_CRM1_1	226	242	PF08389	0.312
TRG_NES_CRM1_1	433	445	PF08389	0.475
TRG_NLS_MonoExtN_4	687	694	PF00514	0.692

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB75	Leptomonas seymouri	63%	98%
A0A3Q8IDJ4	Leishmania donovani	94%	99%
A4HFW3	Leishmania braziliensis	83%	99%
A4I2Z5	Leishmania infantum	94%	99%
E9AZ91	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	99%