BRO1 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

BRO1-like domain/ALIX V-shaped domain binding to HIV, putative

Species:

Leishmania major

UniProt:

E9ADE3_LEIMA

TriTrypDb:

LmjF.27.1640 , LMJLV39_270022800 , LMJSD75_270022900

Length:

914

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 9

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005768	endosome	7	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ADE3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ADE3

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0006886	intracellular protein transport	4	2
GO:0007034	vacuolar transport	4	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016192	vesicle-mediated transport	4	11
GO:0016197	endosomal transport	4	2
GO:0032509	endosome transport via multivesicular body sorting pathway	5	2
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	2
GO:0033036	macromolecule localization	2	2
GO:0033365	protein localization to organelle	5	2
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	2
GO:0045184	establishment of protein localization	3	2
GO:0045324	late endosome to vacuole transport	5	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0071985	multivesicular body sorting pathway	5	11
GO:0072594	establishment of protein localization to organelle	4	2
GO:0072665	protein localization to vacuole	6	2
GO:0072666	establishment of protein localization to vacuole	5	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	297	299	PF00675	0.473
CLV_NRD_NRD_1	413	415	PF00675	0.534
CLV_NRD_NRD_1	505	507	PF00675	0.345
CLV_NRD_NRD_1	778	780	PF00675	0.573
CLV_PCSK_KEX2_1	194	196	PF00082	0.419
CLV_PCSK_KEX2_1	505	507	PF00082	0.362
CLV_PCSK_KEX2_1	72	74	PF00082	0.454
CLV_PCSK_KEX2_1	777	779	PF00082	0.571
CLV_PCSK_PC1ET2_1	194	196	PF00082	0.476
CLV_PCSK_PC1ET2_1	72	74	PF00082	0.457
CLV_PCSK_SKI1_1	107	111	PF00082	0.473
CLV_PCSK_SKI1_1	13	17	PF00082	0.473
CLV_PCSK_SKI1_1	298	302	PF00082	0.462
CLV_PCSK_SKI1_1	735	739	PF00082	0.369
CLV_Separin_Metazoa	445	449	PF03568	0.594
DEG_APCC_DBOX_1	194	202	PF00400	0.462
DEG_SCF_FBW7_2	547	553	PF00400	0.527
DOC_CKS1_1	547	552	PF01111	0.527
DOC_CYCLIN_RxL_1	101	115	PF00134	0.425
DOC_MAPK_gen_1	274	283	PF00069	0.473
DOC_MAPK_gen_1	298	307	PF00069	0.462
DOC_MAPK_gen_1	332	342	PF00069	0.473
DOC_MAPK_gen_1	396	405	PF00069	0.366
DOC_MAPK_MEF2A_6	274	283	PF00069	0.429
DOC_MAPK_NFAT4_5	276	284	PF00069	0.425
DOC_PP1_RVXF_1	105	112	PF00149	0.425
DOC_PP1_RVXF_1	565	571	PF00149	0.504
DOC_PP1_SILK_1	449	454	PF00149	0.551
DOC_PP2B_LxvP_1	305	308	PF13499	0.312
DOC_USP7_MATH_1	336	340	PF00917	0.373
DOC_USP7_MATH_1	413	417	PF00917	0.520
DOC_USP7_MATH_1	446	450	PF00917	0.634
DOC_USP7_MATH_1	51	55	PF00917	0.473
DOC_USP7_MATH_1	75	79	PF00917	0.391
DOC_USP7_MATH_1	761	765	PF00917	0.607
DOC_USP7_MATH_1	815	819	PF00917	0.837
DOC_USP7_MATH_1	837	841	PF00917	0.704
DOC_USP7_MATH_1	900	904	PF00917	0.737
DOC_USP7_MATH_1	99	103	PF00917	0.473
DOC_USP7_UBL2_3	160	164	PF12436	0.347
DOC_USP7_UBL2_3	190	194	PF12436	0.473
DOC_USP7_UBL2_3	660	664	PF12436	0.619
DOC_WW_Pin1_4	457	462	PF00397	0.629
DOC_WW_Pin1_4	523	528	PF00397	0.594
DOC_WW_Pin1_4	546	551	PF00397	0.527
DOC_WW_Pin1_4	7	12	PF00397	0.563
DOC_WW_Pin1_4	71	76	PF00397	0.449
DOC_WW_Pin1_4	802	807	PF00397	0.614
LIG_14-3-3_CanoR_1	195	199	PF00244	0.422
LIG_14-3-3_CanoR_1	309	318	PF00244	0.462
LIG_14-3-3_CanoR_1	34	40	PF00244	0.540
LIG_14-3-3_CanoR_1	370	379	PF00244	0.455
LIG_14-3-3_CanoR_1	414	421	PF00244	0.541
LIG_14-3-3_CanoR_1	735	741	PF00244	0.566
LIG_Actin_WH2_2	478	493	PF00022	0.551
LIG_AP2alpha_2	378	380	PF02296	0.366
LIG_APCC_ABBA_1	548	553	PF00400	0.586
LIG_APCC_ABBA_1	578	583	PF00400	0.536
LIG_BIR_II_1	1	5	PF00653	0.627
LIG_BRCT_BRCA1_1	338	342	PF00533	0.373
LIG_BRCT_BRCA1_1	433	437	PF00533	0.661
LIG_Dynein_DLC8_1	413	419	PF01221	0.578
LIG_EH1_1	465	473	PF00400	0.551
LIG_eIF4E_1	802	808	PF01652	0.584
LIG_FHA_1	175	181	PF00498	0.512
LIG_FHA_1	310	316	PF00498	0.402
LIG_FHA_1	421	427	PF00498	0.515
LIG_FHA_1	43	49	PF00498	0.361
LIG_FHA_1	463	469	PF00498	0.532
LIG_FHA_1	63	69	PF00498	0.196
LIG_FHA_1	686	692	PF00498	0.594
LIG_FHA_2	119	125	PF00498	0.427
LIG_FHA_2	187	193	PF00498	0.473
LIG_FHA_2	326	332	PF00498	0.429
LIG_FHA_2	359	365	PF00498	0.406
LIG_FHA_2	372	378	PF00498	0.445
LIG_FHA_2	392	398	PF00498	0.460
LIG_FHA_2	44	50	PF00498	0.470
LIG_FHA_2	476	482	PF00498	0.523
LIG_FHA_2	555	561	PF00498	0.551
LIG_FHA_2	65	71	PF00498	0.397
LIG_FHA_2	72	78	PF00498	0.360
LIG_LIR_Apic_2	840	846	PF02991	0.709
LIG_LIR_Apic_2	887	893	PF02991	0.770
LIG_LIR_Apic_2	907	911	PF02991	0.751
LIG_LIR_Gen_1	12	21	PF02991	0.353
LIG_LIR_Gen_1	142	151	PF02991	0.347
LIG_LIR_Gen_1	339	350	PF02991	0.347
LIG_LIR_Gen_1	367	372	PF02991	0.467
LIG_LIR_Gen_1	526	536	PF02991	0.558
LIG_LIR_Gen_1	707	717	PF02991	0.504
LIG_LIR_Gen_1	831	839	PF02991	0.693
LIG_LIR_Gen_1	864	872	PF02991	0.678
LIG_LIR_Nem_3	12	17	PF02991	0.410
LIG_LIR_Nem_3	142	148	PF02991	0.347
LIG_LIR_Nem_3	161	166	PF02991	0.347
LIG_LIR_Nem_3	339	345	PF02991	0.347
LIG_LIR_Nem_3	367	371	PF02991	0.356
LIG_LIR_Nem_3	377	383	PF02991	0.379
LIG_LIR_Nem_3	513	519	PF02991	0.553
LIG_LIR_Nem_3	526	532	PF02991	0.509
LIG_LIR_Nem_3	707	712	PF02991	0.504
LIG_LIR_Nem_3	864	868	PF02991	0.681
LIG_MLH1_MIPbox_1	338	342	PF16413	0.366
LIG_MYND_1	859	863	PF01753	0.702
LIG_PDZ_Class_2	909	914	PF00595	0.708
LIG_Pex14_1	113	117	PF04695	0.347
LIG_Pex14_1	516	520	PF04695	0.455
LIG_SH2_CRK	529	533	PF00017	0.527
LIG_SH2_CRK	800	804	PF00017	0.760
LIG_SH2_CRK	823	827	PF00017	0.767
LIG_SH2_CRK	843	847	PF00017	0.610
LIG_SH2_CRK	865	869	PF00017	0.676
LIG_SH2_CRK	87	91	PF00017	0.336
LIG_SH2_CRK	890	894	PF00017	0.795
LIG_SH2_CRK	908	912	PF00017	0.748
LIG_SH2_GRB2like	58	61	PF00017	0.462
LIG_SH2_NCK_1	137	141	PF00017	0.347
LIG_SH2_NCK_1	173	177	PF00017	0.347
LIG_SH2_NCK_1	865	869	PF00017	0.756
LIG_SH2_PTP2	143	146	PF00017	0.457
LIG_SH2_SRC	143	146	PF00017	0.425
LIG_SH2_SRC	239	242	PF00017	0.366
LIG_SH2_SRC	507	510	PF00017	0.594
LIG_SH2_SRC	800	803	PF00017	0.733
LIG_SH2_SRC	823	826	PF00017	0.696
LIG_SH2_SRC	843	846	PF00017	0.656
LIG_SH2_SRC	890	893	PF00017	0.778
LIG_SH2_STAP1	239	243	PF00017	0.366
LIG_SH2_STAT5	143	146	PF00017	0.374
LIG_SH2_STAT5	214	217	PF00017	0.347
LIG_SH2_STAT5	341	344	PF00017	0.347
LIG_SH2_STAT5	35	38	PF00017	0.425
LIG_SH2_STAT5	507	510	PF00017	0.552
LIG_SH2_STAT5	529	532	PF00017	0.588
LIG_SH2_STAT5	740	743	PF00017	0.639
LIG_SH2_STAT5	87	90	PF00017	0.347
LIG_SH3_1	800	806	PF00018	0.678
LIG_SH3_3	346	352	PF00018	0.366
LIG_SH3_3	440	446	PF00018	0.425
LIG_SH3_3	800	806	PF00018	0.700
LIG_SH3_3	808	814	PF00018	0.658
LIG_SH3_3	856	862	PF00018	0.783
LIG_SH3_3	873	879	PF00018	0.586
LIG_TRAF2_1	557	560	PF00917	0.527
LIG_TRAF2_1	616	619	PF00917	0.537
LIG_TYR_ITIM	85	90	PF00017	0.473
LIG_UBA3_1	451	459	PF00899	0.594
LIG_WRC_WIRS_1	243	248	PF05994	0.462
LIG_WW_1	862	865	PF00397	0.658
LIG_WW_2	859	862	PF00397	0.701
MOD_CDK_SPxK_1	7	13	PF00069	0.549
MOD_CK1_1	125	131	PF00069	0.473
MOD_CK1_1	245	251	PF00069	0.418
MOD_CK1_1	30	36	PF00069	0.397
MOD_CK1_1	321	327	PF00069	0.396
MOD_CK1_1	358	364	PF00069	0.415
MOD_CK1_1	416	422	PF00069	0.550
MOD_CK1_1	42	48	PF00069	0.289
MOD_CK1_1	462	468	PF00069	0.590
MOD_CK1_1	523	529	PF00069	0.515
MOD_CK1_1	637	643	PF00069	0.599
MOD_CK1_1	71	77	PF00069	0.462
MOD_CK2_1	113	119	PF00069	0.459
MOD_CK2_1	125	131	PF00069	0.376
MOD_CK2_1	358	364	PF00069	0.388
MOD_CK2_1	371	377	PF00069	0.394
MOD_CK2_1	391	397	PF00069	0.460
MOD_CK2_1	475	481	PF00069	0.531
MOD_CK2_1	534	540	PF00069	0.625
MOD_CK2_1	554	560	PF00069	0.396
MOD_CK2_1	633	639	PF00069	0.504
MOD_CK2_1	64	70	PF00069	0.389
MOD_CK2_1	71	77	PF00069	0.339
MOD_GlcNHglycan	1	4	PF01048	0.742
MOD_GlcNHglycan	101	104	PF01048	0.473
MOD_GlcNHglycan	324	327	PF01048	0.499
MOD_GlcNHglycan	433	436	PF01048	0.536
MOD_GlcNHglycan	461	464	PF01048	0.422
MOD_GlcNHglycan	492	496	PF01048	0.442
MOD_GlcNHglycan	532	535	PF01048	0.361
MOD_GlcNHglycan	590	593	PF01048	0.394
MOD_GlcNHglycan	643	646	PF01048	0.295
MOD_GlcNHglycan	691	694	PF01048	0.427
MOD_GlcNHglycan	839	842	PF01048	0.698
MOD_GlcNHglycan	902	905	PF01048	0.773
MOD_GSK3_1	113	120	PF00069	0.312
MOD_GSK3_1	123	130	PF00069	0.399
MOD_GSK3_1	146	153	PF00069	0.506
MOD_GSK3_1	241	248	PF00069	0.350
MOD_GSK3_1	309	316	PF00069	0.391
MOD_GSK3_1	318	325	PF00069	0.347
MOD_GSK3_1	35	42	PF00069	0.361
MOD_GSK3_1	360	367	PF00069	0.343
MOD_GSK3_1	416	423	PF00069	0.537
MOD_GSK3_1	462	469	PF00069	0.565
MOD_GSK3_1	471	478	PF00069	0.520
MOD_GSK3_1	530	537	PF00069	0.622
MOD_GSK3_1	58	65	PF00069	0.391
MOD_GSK3_1	633	640	PF00069	0.535
MOD_GSK3_1	648	655	PF00069	0.464
MOD_GSK3_1	681	688	PF00069	0.594
MOD_GSK3_1	71	78	PF00069	0.426
MOD_GSK3_1	736	743	PF00069	0.519
MOD_GSK3_1	866	873	PF00069	0.788
MOD_GSK3_1	900	907	PF00069	0.730
MOD_GSK3_1	99	106	PF00069	0.442
MOD_LATS_1	412	418	PF00433	0.565
MOD_LATS_1	623	629	PF00433	0.551
MOD_N-GLC_1	336	341	PF02516	0.347
MOD_N-GLC_1	42	47	PF02516	0.366
MOD_N-GLC_1	457	462	PF02516	0.394
MOD_N-GLC_1	7	12	PF02516	0.469
MOD_N-GLC_1	75	80	PF02516	0.442
MOD_N-GLC_2	269	271	PF02516	0.362
MOD_N-GLC_2	404	406	PF02516	0.574
MOD_N-GLC_2	477	479	PF02516	0.385
MOD_N-GLC_2	704	706	PF02516	0.318
MOD_NEK2_1	117	122	PF00069	0.373
MOD_NEK2_1	139	144	PF00069	0.347
MOD_NEK2_1	175	180	PF00069	0.473
MOD_NEK2_1	246	251	PF00069	0.393
MOD_NEK2_1	371	376	PF00069	0.439
MOD_NEK2_1	447	452	PF00069	0.594
MOD_NEK2_1	633	638	PF00069	0.517
MOD_NEK2_1	68	73	PF00069	0.447
MOD_NEK2_2	217	222	PF00069	0.462
MOD_NEK2_2	336	341	PF00069	0.425
MOD_PIKK_1	146	152	PF00454	0.473
MOD_PIKK_1	309	315	PF00454	0.462
MOD_PIKK_1	414	420	PF00454	0.597
MOD_PIKK_1	638	644	PF00454	0.526
MOD_PIKK_1	761	767	PF00454	0.647
MOD_PIKK_1	768	774	PF00454	0.591
MOD_PKA_1	194	200	PF00069	0.442
MOD_PKA_1	414	420	PF00069	0.597
MOD_PKA_2	194	200	PF00069	0.498
MOD_PKA_2	308	314	PF00069	0.429
MOD_PKA_2	413	419	PF00069	0.547
MOD_PKA_2	447	453	PF00069	0.593
MOD_Plk_1	217	223	PF00069	0.397
MOD_Plk_1	27	33	PF00069	0.404
MOD_Plk_1	336	342	PF00069	0.347
MOD_Plk_1	43	49	PF00069	0.260
MOD_Plk_1	730	736	PF00069	0.594
MOD_Plk_1	75	81	PF00069	0.384
MOD_Plk_4	139	145	PF00069	0.412
MOD_Plk_4	175	181	PF00069	0.473
MOD_Plk_4	246	252	PF00069	0.419
MOD_Plk_4	313	319	PF00069	0.386
MOD_Plk_4	336	342	PF00069	0.431
MOD_Plk_4	364	370	PF00069	0.408
MOD_Plk_4	447	453	PF00069	0.582
MOD_Plk_4	58	64	PF00069	0.363
MOD_Plk_4	704	710	PF00069	0.518
MOD_Plk_4	715	721	PF00069	0.518
MOD_Plk_4	75	81	PF00069	0.288
MOD_ProDKin_1	457	463	PF00069	0.629
MOD_ProDKin_1	523	529	PF00069	0.594
MOD_ProDKin_1	546	552	PF00069	0.527
MOD_ProDKin_1	7	13	PF00069	0.560
MOD_ProDKin_1	71	77	PF00069	0.449
MOD_ProDKin_1	802	808	PF00069	0.617
MOD_SUMO_for_1	215	218	PF00179	0.347
MOD_SUMO_for_1	283	286	PF00179	0.329
MOD_SUMO_for_1	673	676	PF00179	0.551
MOD_SUMO_rev_2	155	162	PF00179	0.401
MOD_SUMO_rev_2	325	333	PF00179	0.462
MOD_SUMO_rev_2	456	461	PF00179	0.586
MOD_SUMO_rev_2	481	490	PF00179	0.600
MOD_SUMO_rev_2	603	609	PF00179	0.570
MOD_SUMO_rev_2	612	622	PF00179	0.517
TRG_DiLeu_BaEn_2	363	369	PF01217	0.460
TRG_DiLeu_BaLyEn_6	803	808	PF01217	0.585
TRG_ENDOCYTIC_2	143	146	PF00928	0.425
TRG_ENDOCYTIC_2	163	166	PF00928	0.196
TRG_ENDOCYTIC_2	172	175	PF00928	0.307
TRG_ENDOCYTIC_2	239	242	PF00928	0.366
TRG_ENDOCYTIC_2	529	532	PF00928	0.509
TRG_ENDOCYTIC_2	709	712	PF00928	0.504
TRG_ENDOCYTIC_2	832	835	PF00928	0.811
TRG_ENDOCYTIC_2	865	868	PF00928	0.660
TRG_ENDOCYTIC_2	87	90	PF00928	0.347
TRG_ER_diArg_1	440	443	PF00400	0.604
TRG_ER_diArg_1	505	507	PF00400	0.568
TRG_ER_diArg_1	777	779	PF00400	0.640
TRG_Pf-PMV_PEXEL_1	556	560	PF00026	0.338
TRG_Pf-PMV_PEXEL_1	602	606	PF00026	0.372
TRG_Pf-PMV_PEXEL_1	744	749	PF00026	0.397

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2T8	Leptomonas seymouri	75%	100%
A0A1X0P4J9	Trypanosomatidae	49%	100%
A0A3S7X0S6	Leishmania donovani	96%	100%
A0A422N9P5	Trypanosoma rangeli	47%	100%
A4HFU0	Leishmania braziliensis	86%	89%
A4I2V6	Leishmania infantum	96%	100%
D0A635	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
E9AZ67	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
P79020	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	22%	100%
Q4X0Z5	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	20%	94%
Q8T7K0	Dictyostelium discoideum	21%	100%
Q8WUM4	Homo sapiens	24%	100%
Q9QZA2	Rattus norvegicus	24%	100%
Q9WU78	Mus musculus	25%	100%
V5BQH3	Trypanosoma cruzi	50%	100%