Uncharacterized protein

Quick info Annotations Localization Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AD82_LEIMA

TriTrypDb:

LmjF.27.1045 , LMJLV39_270016400 , LMJSD75_270016600

Length:

146

Annotations

LeishMANIAdb annotations

This small membrane protein has a very long, basic N-terminus that might indicate a putative mitochondrial transit signal.. The protein is conserved in all KInetoplastids, but not seen outside this group.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	9
GO:0110165	cellular anatomical entity	1	9

Expansion

Sequence features

E9AD82

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AD82

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	130	132	PF00675	0.547
CLV_NRD_NRD_1	134	136	PF00675	0.554
CLV_NRD_NRD_1	18	20	PF00675	0.446
CLV_NRD_NRD_1	21	23	PF00675	0.460
CLV_NRD_NRD_1	3	5	PF00675	0.447
CLV_PCSK_FUR_1	131	135	PF00082	0.590
CLV_PCSK_FUR_1	18	22	PF00082	0.424
CLV_PCSK_KEX2_1	130	132	PF00082	0.549
CLV_PCSK_KEX2_1	133	135	PF00082	0.557
CLV_PCSK_KEX2_1	18	20	PF00082	0.445
CLV_PCSK_KEX2_1	21	23	PF00082	0.461
CLV_PCSK_KEX2_1	3	5	PF00082	0.447
CLV_PCSK_PC1ET2_1	20	22	PF00082	0.441
CLV_PCSK_PC7_1	130	136	PF00082	0.581
CLV_Separin_Metazoa	10	14	PF03568	0.656
DEG_Nend_Nbox_1	1	3	PF02207	0.634
DOC_MAPK_gen_1	111	120	PF00069	0.375
DOC_MAPK_gen_1	3	11	PF00069	0.648
DOC_MAPK_MEF2A_6	114	122	PF00069	0.407
DOC_MAPK_MEF2A_6	3	11	PF00069	0.644
DOC_MAPK_RevD_3	7	20	PF00069	0.590
DOC_PP2B_LxvP_1	57	60	PF13499	0.668
LIG_14-3-3_CanoR_1	68	73	PF00244	0.594
LIG_FHA_2	94	100	PF00498	0.447
LIG_LIR_Gen_1	105	113	PF02991	0.445
LIG_LIR_Nem_3	105	110	PF02991	0.414
LIG_LIR_Nem_3	119	125	PF02991	0.291
LIG_LIR_Nem_3	81	86	PF02991	0.294
LIG_REV1ctd_RIR_1	90	98	PF16727	0.550
LIG_SH2_CRK	107	111	PF00017	0.347
LIG_SH2_STAT3	50	53	PF00017	0.621
LIG_SH2_STAT5	107	110	PF00017	0.395
LIG_SH2_STAT5	50	53	PF00017	0.527
LIG_SH2_STAT5	86	89	PF00017	0.284
LIG_SH2_STAT5	95	98	PF00017	0.350
LIG_SH3_3	35	41	PF00018	0.766
LIG_SH3_3	8	14	PF00018	0.694
LIG_TYR_ITSM	103	110	PF00017	0.450
MOD_CK2_1	93	99	PF00069	0.371
MOD_Cter_Amidation	128	131	PF01082	0.595
MOD_GlcNHglycan	45	48	PF01048	0.399
MOD_PIKK_1	102	108	PF00454	0.454
MOD_PKA_2	67	73	PF00069	0.615
MOD_Plk_4	105	111	PF00069	0.364
MOD_Plk_4	79	85	PF00069	0.339
TRG_DiLeu_BaLyEn_6	135	140	PF01217	0.408
TRG_ENDOCYTIC_2	107	110	PF00928	0.265
TRG_ENDOCYTIC_2	89	92	PF00928	0.351
TRG_ER_diArg_1	130	133	PF00400	0.365
TRG_ER_diArg_1	18	21	PF00400	0.620
TRG_ER_diArg_1	2	4	PF00400	0.648
TRG_NLS_Bipartite_1	3	24	PF00514	0.662
TRG_NLS_MonoCore_2	18	23	PF00514	0.648
TRG_NLS_MonoExtC_3	19	24	PF00514	0.726
TRG_NLS_MonoExtN_4	18	24	PF00514	0.658
TRG_Pf-PMV_PEXEL_1	138	143	PF00026	0.610

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZT6	Leptomonas seymouri	73%	100%
A0A0S4JFD9	Bodo saltans	46%	100%
A0A1X0P4W3	Trypanosomatidae	53%	100%
A0A3S7X0M9	Leishmania donovani	96%	100%
A0A422MWH1	Trypanosoma rangeli	53%	100%
A4HFM8	Leishmania braziliensis	85%	100%
A4I2Q6	Leishmania infantum	96%	100%
D0A5V7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	100%
E9AZ06	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%