Pep3_Vps18 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Pep3/Vps18/deep orange family/Region in Clathrin and VPS, putative

Species:

Leishmania major

UniProt:

E9AD68_LEIMA

TriTrypDb:

LmjF.27.0910 , LMJLV39_270014800 , LMJSD75_270014800

Length:

1091

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005768	endosome	7	2
GO:0030897	HOPS complex	3	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0098796	membrane protein complex	2	2
GO:0099023	vesicle tethering complex	2	2
GO:0110165	cellular anatomical entity	1	11
GO:0016020	membrane	2	9

Expansion

Sequence features

E9AD68

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AD68

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	10
GO:0006886	intracellular protein transport	4	10
GO:0006904	vesicle docking involved in exocytosis	3	2
GO:0006996	organelle organization	4	2
GO:0007032	endosome organization	6	2
GO:0007033	vacuole organization	5	2
GO:0008104	protein localization	4	10
GO:0009987	cellular process	1	10
GO:0015031	protein transport	4	10
GO:0016043	cellular component organization	3	2
GO:0016050	vesicle organization	5	2
GO:0022406	membrane docking	2	2
GO:0033036	macromolecule localization	2	10
GO:0045184	establishment of protein localization	3	10
GO:0046907	intracellular transport	3	10
GO:0048278	vesicle docking	4	2
GO:0048284	organelle fusion	5	2
GO:0051179	localization	1	10
GO:0051234	establishment of localization	2	10
GO:0051640	organelle localization	2	2
GO:0051641	cellular localization	2	10
GO:0051649	establishment of localization in cell	3	10
GO:0070727	cellular macromolecule localization	3	10
GO:0071702	organic substance transport	4	10
GO:0071705	nitrogen compound transport	4	10
GO:0071840	cellular component organization or biogenesis	2	2
GO:0140029	exocytic process	2	2
GO:0140056	organelle localization by membrane tethering	3	2
GO:0016192	vesicle-mediated transport	4	8

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0030674	protein-macromolecule adaptor activity	2	2
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0060090	molecular adaptor activity	1	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	33	37	PF00656	0.605
CLV_C14_Caspase3-7	58	62	PF00656	0.521
CLV_C14_Caspase3-7	757	761	PF00656	0.576
CLV_C14_Caspase3-7	91	95	PF00656	0.475
CLV_NRD_NRD_1	1083	1085	PF00675	0.506
CLV_NRD_NRD_1	295	297	PF00675	0.332
CLV_NRD_NRD_1	403	405	PF00675	0.337
CLV_NRD_NRD_1	466	468	PF00675	0.383
CLV_NRD_NRD_1	505	507	PF00675	0.324
CLV_NRD_NRD_1	52	54	PF00675	0.528
CLV_NRD_NRD_1	530	532	PF00675	0.500
CLV_NRD_NRD_1	853	855	PF00675	0.723
CLV_NRD_NRD_1	936	938	PF00675	0.429
CLV_PCSK_KEX2_1	295	297	PF00082	0.332
CLV_PCSK_KEX2_1	403	405	PF00082	0.407
CLV_PCSK_KEX2_1	466	468	PF00082	0.419
CLV_PCSK_KEX2_1	507	509	PF00082	0.323
CLV_PCSK_KEX2_1	52	54	PF00082	0.528
CLV_PCSK_KEX2_1	530	532	PF00082	0.500
CLV_PCSK_KEX2_1	853	855	PF00082	0.723
CLV_PCSK_KEX2_1	963	965	PF00082	0.419
CLV_PCSK_PC1ET2_1	507	509	PF00082	0.341
CLV_PCSK_PC1ET2_1	963	965	PF00082	0.419
CLV_PCSK_SKI1_1	1005	1009	PF00082	0.341
CLV_PCSK_SKI1_1	205	209	PF00082	0.550
CLV_PCSK_SKI1_1	290	294	PF00082	0.474
CLV_PCSK_SKI1_1	332	336	PF00082	0.579
CLV_PCSK_SKI1_1	490	494	PF00082	0.357
CLV_PCSK_SKI1_1	515	519	PF00082	0.456
CLV_PCSK_SKI1_1	570	574	PF00082	0.389
CLV_PCSK_SKI1_1	626	630	PF00082	0.486
CLV_PCSK_SKI1_1	66	70	PF00082	0.509
CLV_PCSK_SKI1_1	725	729	PF00082	0.459
CLV_PCSK_SKI1_1	874	878	PF00082	0.563
DEG_APCC_DBOX_1	472	480	PF00400	0.454
DEG_APCC_DBOX_1	65	73	PF00400	0.565
DEG_APCC_KENBOX_2	639	643	PF00400	0.330
DEG_COP1_1	337	348	PF00400	0.610
DEG_SPOP_SBC_1	248	252	PF00917	0.533
DOC_ANK_TNKS_1	416	423	PF00023	0.220
DOC_CYCLIN_RxL_1	623	633	PF00134	0.528
DOC_CYCLIN_RxL_1	729	742	PF00134	0.555
DOC_CYCLIN_RxL_1	999	1012	PF00134	0.347
DOC_CYCLIN_yCln2_LP_2	16	22	PF00134	0.591
DOC_MAPK_DCC_7	732	741	PF00069	0.562
DOC_MAPK_FxFP_2	1015	1018	PF00069	0.392
DOC_MAPK_gen_1	201	209	PF00069	0.316
DOC_MAPK_gen_1	269	277	PF00069	0.545
DOC_MAPK_gen_1	378	388	PF00069	0.336
DOC_MAPK_gen_1	52	59	PF00069	0.508
DOC_MAPK_gen_1	729	739	PF00069	0.562
DOC_MAPK_gen_1	937	944	PF00069	0.454
DOC_MAPK_MEF2A_6	381	390	PF00069	0.217
DOC_MAPK_MEF2A_6	732	741	PF00069	0.562
DOC_PP1_RVXF_1	221	228	PF00149	0.464
DOC_PP1_RVXF_1	330	336	PF00149	0.577
DOC_PP1_RVXF_1	438	444	PF00149	0.307
DOC_PP1_RVXF_1	513	519	PF00149	0.480
DOC_PP2B_LxvP_1	847	850	PF13499	0.602
DOC_PP4_FxxP_1	1015	1018	PF00568	0.392
DOC_PP4_FxxP_1	429	432	PF00568	0.454
DOC_PP4_FxxP_1	80	83	PF00568	0.378
DOC_USP7_MATH_1	123	127	PF00917	0.462
DOC_USP7_MATH_1	139	143	PF00917	0.560
DOC_USP7_MATH_1	221	225	PF00917	0.428
DOC_USP7_MATH_1	248	252	PF00917	0.531
DOC_USP7_MATH_1	262	266	PF00917	0.534
DOC_USP7_MATH_1	412	416	PF00917	0.440
DOC_USP7_MATH_1	499	503	PF00917	0.407
DOC_USP7_MATH_1	868	872	PF00917	0.741
DOC_USP7_MATH_1	910	914	PF00917	0.586
DOC_WW_Pin1_4	1009	1014	PF00397	0.354
DOC_WW_Pin1_4	211	216	PF00397	0.467
DOC_WW_Pin1_4	415	420	PF00397	0.416
DOC_WW_Pin1_4	446	451	PF00397	0.401
LIG_14-3-3_CanoR_1	271	276	PF00244	0.569
LIG_14-3-3_CanoR_1	295	299	PF00244	0.447
LIG_14-3-3_CanoR_1	336	341	PF00244	0.701
LIG_AP2alpha_2	444	446	PF02296	0.322
LIG_APCC_ABBA_1	20	25	PF00400	0.544
LIG_APCC_ABBA_1	836	841	PF00400	0.307
LIG_BIR_II_1	1	5	PF00653	0.688
LIG_BIR_III_3	1	5	PF00653	0.697
LIG_BRCT_BRCA1_1	184	188	PF00533	0.519
LIG_BRCT_BRCA1_1	216	220	PF00533	0.621
LIG_BRCT_BRCA1_1	223	227	PF00533	0.197
LIG_EH1_1	763	771	PF00400	0.494
LIG_FHA_1	108	114	PF00498	0.554
LIG_FHA_1	265	271	PF00498	0.501
LIG_FHA_1	272	278	PF00498	0.516
LIG_FHA_1	322	328	PF00498	0.543
LIG_FHA_1	335	341	PF00498	0.646
LIG_FHA_1	569	575	PF00498	0.454
LIG_FHA_1	669	675	PF00498	0.444
LIG_FHA_1	706	712	PF00498	0.451
LIG_FHA_1	718	724	PF00498	0.289
LIG_FHA_1	825	831	PF00498	0.465
LIG_FHA_1	84	90	PF00498	0.495
LIG_FHA_1	842	848	PF00498	0.285
LIG_FHA_1	930	936	PF00498	0.427
LIG_FHA_2	89	95	PF00498	0.450
LIG_FHA_2	967	973	PF00498	0.410
LIG_FHA_2	974	980	PF00498	0.383
LIG_Integrin_isoDGR_2	288	290	PF01839	0.541
LIG_LIR_Apic_2	1012	1018	PF02991	0.415
LIG_LIR_Apic_2	415	419	PF02991	0.429
LIG_LIR_Apic_2	427	432	PF02991	0.264
LIG_LIR_Gen_1	174	181	PF02991	0.483
LIG_LIR_Gen_1	393	402	PF02991	0.307
LIG_LIR_Gen_1	474	485	PF02991	0.405
LIG_LIR_Gen_1	552	561	PF02991	0.386
LIG_LIR_Gen_1	575	586	PF02991	0.501
LIG_LIR_Gen_1	596	607	PF02991	0.484
LIG_LIR_Gen_1	621	632	PF02991	0.527
LIG_LIR_Gen_1	706	712	PF02991	0.484
LIG_LIR_Gen_1	86	92	PF02991	0.439
LIG_LIR_Nem_3	1011	1017	PF02991	0.306
LIG_LIR_Nem_3	174	178	PF02991	0.500
LIG_LIR_Nem_3	393	398	PF02991	0.307
LIG_LIR_Nem_3	474	480	PF02991	0.374
LIG_LIR_Nem_3	552	557	PF02991	0.375
LIG_LIR_Nem_3	575	581	PF02991	0.374
LIG_LIR_Nem_3	596	602	PF02991	0.480
LIG_LIR_Nem_3	621	627	PF02991	0.537
LIG_LIR_Nem_3	706	710	PF02991	0.369
LIG_LIR_Nem_3	747	751	PF02991	0.468
LIG_LIR_Nem_3	86	90	PF02991	0.480
LIG_LIR_Nem_3	946	951	PF02991	0.433
LIG_LIR_Nem_3	954	959	PF02991	0.359
LIG_LIR_Nem_3	989	994	PF02991	0.394
LIG_PCNA_yPIPBox_3	44	56	PF02747	0.438
LIG_PCNA_yPIPBox_3	893	903	PF02747	0.529
LIG_Rb_pABgroove_1	312	320	PF01858	0.409
LIG_SH2_CRK	558	562	PF00017	0.370
LIG_SH2_CRK	788	792	PF00017	0.220
LIG_SH2_NCK_1	298	302	PF00017	0.471
LIG_SH2_PTP2	87	90	PF00017	0.433
LIG_SH2_SRC	751	754	PF00017	0.469
LIG_SH2_SRC	848	851	PF00017	0.525
LIG_SH2_STAP1	645	649	PF00017	0.464
LIG_SH2_STAP1	751	755	PF00017	0.554
LIG_SH2_STAP1	788	792	PF00017	0.220
LIG_SH2_STAP1	815	819	PF00017	0.337
LIG_SH2_STAT3	959	962	PF00017	0.454
LIG_SH2_STAT5	1014	1017	PF00017	0.355
LIG_SH2_STAT5	177	180	PF00017	0.443
LIG_SH2_STAT5	305	308	PF00017	0.490
LIG_SH2_STAT5	469	472	PF00017	0.221
LIG_SH2_STAT5	673	676	PF00017	0.372
LIG_SH2_STAT5	848	851	PF00017	0.436
LIG_SH2_STAT5	87	90	PF00017	0.469
LIG_SH2_STAT5	955	958	PF00017	0.380
LIG_SH2_STAT5	993	996	PF00017	0.307
LIG_SH3_1	428	434	PF00018	0.454
LIG_SH3_3	1075	1081	PF00018	0.546
LIG_SH3_3	16	22	PF00018	0.591
LIG_SH3_3	24	30	PF00018	0.531
LIG_SH3_3	396	402	PF00018	0.442
LIG_SH3_3	428	434	PF00018	0.454
LIG_SH3_3	70	76	PF00018	0.477
LIG_SUMO_SIM_par_1	1074	1079	PF11976	0.432
LIG_SUMO_SIM_par_1	235	241	PF11976	0.460
LIG_SUMO_SIM_par_1	715	721	PF11976	0.488
LIG_SUMO_SIM_par_1	765	771	PF11976	0.462
LIG_TRAF2_1	976	979	PF00917	0.418
LIG_UBA3_1	598	606	PF00899	0.481
LIG_UBA3_1	723	729	PF00899	0.536
LIG_WRC_WIRS_1	1039	1044	PF05994	0.500
LIG_WRC_WIRS_1	745	750	PF05994	0.523
LIG_WW_2	431	434	PF00397	0.454
MOD_CK1_1	160	166	PF00069	0.618
MOD_CK1_1	211	217	PF00069	0.509
MOD_CK1_1	338	344	PF00069	0.629
MOD_CK1_1	415	421	PF00069	0.389
MOD_CK2_1	1028	1034	PF00069	0.437
MOD_CK2_1	280	286	PF00069	0.590
MOD_CK2_1	336	342	PF00069	0.686
MOD_CK2_1	344	350	PF00069	0.705
MOD_CK2_1	627	633	PF00069	0.411
MOD_CK2_1	751	757	PF00069	0.491
MOD_CK2_1	8	14	PF00069	0.672
MOD_CK2_1	90	96	PF00069	0.431
MOD_CK2_1	966	972	PF00069	0.409
MOD_CK2_1	973	979	PF00069	0.389
MOD_GlcNHglycan	159	162	PF01048	0.685
MOD_GlcNHglycan	184	187	PF01048	0.491
MOD_GlcNHglycan	264	267	PF01048	0.528
MOD_GlcNHglycan	280	283	PF01048	0.435
MOD_GlcNHglycan	308	311	PF01048	0.464
MOD_GlcNHglycan	383	386	PF01048	0.368
MOD_GlcNHglycan	53	56	PF01048	0.503
MOD_GlcNHglycan	612	615	PF01048	0.550
MOD_GlcNHglycan	616	619	PF01048	0.516
MOD_GlcNHglycan	659	662	PF01048	0.510
MOD_GlcNHglycan	696	699	PF01048	0.536
MOD_GlcNHglycan	780	783	PF01048	0.429
MOD_GlcNHglycan	855	858	PF01048	0.633
MOD_GlcNHglycan	925	928	PF01048	0.353
MOD_GSK3_1	1003	1010	PF00069	0.426
MOD_GSK3_1	1028	1035	PF00069	0.535
MOD_GSK3_1	157	164	PF00069	0.631
MOD_GSK3_1	25	32	PF00069	0.530
MOD_GSK3_1	334	341	PF00069	0.599
MOD_GSK3_1	446	453	PF00069	0.376
MOD_GSK3_1	560	567	PF00069	0.447
MOD_GSK3_1	580	587	PF00069	0.522
MOD_GSK3_1	610	617	PF00069	0.495
MOD_GSK3_1	95	102	PF00069	0.461
MOD_LATS_1	851	857	PF00433	0.507
MOD_N-GLC_1	8	13	PF02516	0.463
MOD_N-GLC_2	525	527	PF02516	0.434
MOD_NEK2_1	1007	1012	PF00069	0.344
MOD_NEK2_1	107	112	PF00069	0.559
MOD_NEK2_1	208	213	PF00069	0.513
MOD_NEK2_1	227	232	PF00069	0.504
MOD_NEK2_1	237	242	PF00069	0.359
MOD_NEK2_1	334	339	PF00069	0.553
MOD_NEK2_1	471	476	PF00069	0.408
MOD_NEK2_1	51	56	PF00069	0.448
MOD_NEK2_1	560	565	PF00069	0.400
MOD_NEK2_1	580	585	PF00069	0.535
MOD_NEK2_1	627	632	PF00069	0.404
MOD_NEK2_1	668	673	PF00069	0.501
MOD_NEK2_1	68	73	PF00069	0.525
MOD_NEK2_1	804	809	PF00069	0.316
MOD_NEK2_1	810	815	PF00069	0.308
MOD_NEK2_2	883	888	PF00069	0.534
MOD_PIKK_1	108	114	PF00454	0.464
MOD_PIKK_1	123	129	PF00454	0.641
MOD_PIKK_1	161	167	PF00454	0.640
MOD_PIKK_1	269	275	PF00454	0.571
MOD_PIKK_1	31	37	PF00454	0.599
MOD_PIKK_1	519	525	PF00454	0.530
MOD_PIKK_1	966	972	PF00454	0.372
MOD_PIKK_1	994	1000	PF00454	0.412
MOD_PK_1	1032	1038	PF00069	0.443
MOD_PK_1	336	342	PF00069	0.618
MOD_PKA_1	853	859	PF00069	0.704
MOD_PKA_2	294	300	PF00069	0.521
MOD_PKA_2	335	341	PF00069	0.634
MOD_PKA_2	51	57	PF00069	0.460
MOD_PKA_2	564	570	PF00069	0.491
MOD_PKA_2	853	859	PF00069	0.701
MOD_Plk_1	1085	1091	PF00069	0.515
MOD_Plk_1	173	179	PF00069	0.542
MOD_Plk_1	341	347	PF00069	0.662
MOD_Plk_1	356	362	PF00069	0.547
MOD_Plk_1	705	711	PF00069	0.447
MOD_Plk_1	751	757	PF00069	0.548
MOD_Plk_1	8	14	PF00069	0.673
MOD_Plk_1	863	869	PF00069	0.674
MOD_Plk_1	883	889	PF00069	0.535
MOD_Plk_1	95	101	PF00069	0.500
MOD_Plk_4	1038	1044	PF00069	0.493
MOD_Plk_4	301	307	PF00069	0.455
MOD_Plk_4	594	600	PF00069	0.479
MOD_Plk_4	706	712	PF00069	0.461
MOD_Plk_4	744	750	PF00069	0.486
MOD_Plk_4	805	811	PF00069	0.387
MOD_Plk_4	83	89	PF00069	0.475
MOD_ProDKin_1	1009	1015	PF00069	0.354
MOD_ProDKin_1	211	217	PF00069	0.476
MOD_ProDKin_1	415	421	PF00069	0.416
MOD_ProDKin_1	446	452	PF00069	0.401
MOD_SUMO_rev_2	866	876	PF00179	0.642
TRG_DiLeu_BaEn_1	174	179	PF01217	0.442
TRG_DiLeu_BaEn_1	329	334	PF01217	0.570
TRG_DiLeu_BaEn_1	64	69	PF01217	0.590
TRG_DiLeu_BaEn_1	940	945	PF01217	0.376
TRG_DiLeu_BaLyEn_6	647	652	PF01217	0.398
TRG_DiLeu_BaLyEn_6	787	792	PF01217	0.376
TRG_DiLeu_LyEn_5	329	334	PF01217	0.570
TRG_ENDOCYTIC_2	558	561	PF00928	0.363
TRG_ENDOCYTIC_2	645	648	PF00928	0.389
TRG_ENDOCYTIC_2	788	791	PF00928	0.302
TRG_ENDOCYTIC_2	833	836	PF00928	0.307
TRG_ENDOCYTIC_2	87	90	PF00928	0.483
TRG_ER_diArg_1	294	296	PF00400	0.320
TRG_ER_diArg_1	402	404	PF00400	0.337
TRG_ER_diArg_1	440	443	PF00400	0.324
TRG_ER_diArg_1	466	468	PF00400	0.427
TRG_ER_diArg_1	505	508	PF00400	0.307
TRG_ER_diArg_1	51	53	PF00400	0.503
TRG_ER_diArg_1	529	531	PF00400	0.396
TRG_ER_diArg_1	670	673	PF00400	0.443
TRG_ER_FFAT_1	950	961	PF00635	0.437
TRG_NES_CRM1_1	594	608	PF08389	0.427
TRG_NES_CRM1_1	934	947	PF08389	0.368
TRG_NLS_MonoExtC_3	505	511	PF00514	0.427
TRG_Pf-PMV_PEXEL_1	1005	1009	PF00026	0.374
TRG_Pf-PMV_PEXEL_1	650	654	PF00026	0.432
TRG_Pf-PMV_PEXEL_1	725	730	PF00026	0.515
TRG_Pf-PMV_PEXEL_1	735	740	PF00026	0.559
TRG_Pf-PMV_PEXEL_1	790	794	PF00026	0.428
TRG_Pf-PMV_PEXEL_1	937	941	PF00026	0.463

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PAX5	Leptomonas seymouri	63%	99%
A0A0S4JVP2	Bodo saltans	30%	100%
A0A1X0P439	Trypanosomatidae	35%	98%
A0A3R7NS00	Trypanosoma rangeli	36%	100%
A0A3S7X0J6	Leishmania donovani	95%	100%
A4HFL4	Leishmania braziliensis	81%	100%
A4I2P5	Leishmania infantum	96%	100%
D0A5Q6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	99%
E9AYZ3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
F4IDS7	Arabidopsis thaliana	24%	100%
P59015	Danio rerio	22%	100%
V5BE49	Trypanosoma cruzi	39%	100%