EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Repeat of unknown function (DUF1126)/EF-hand domain pair, putative

Species:

Leishmania major

UniProt:

E9AD64_LEIMA

TriTrypDb:

LmjF.27.0870 , LMJLV39_270014400 , LMJSD75_270014400

Length:

756

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005819	spindle	5	2
GO:0005930	axoneme	2	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0072686	mitotic spindle	6	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AD64

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AD64

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	2
GO:0000281	mitotic cytokinesis	4	2
GO:0000910	cytokinesis	3	2
GO:0001539	cilium or flagellum-dependent cell motility	3	2
GO:0006996	organelle organization	4	2
GO:0007010	cytoskeleton organization	5	2
GO:0007017	microtubule-based process	2	2
GO:0007051	spindle organization	3	2
GO:0007052	mitotic spindle organization	4	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0022402	cell cycle process	2	2
GO:0048870	cell motility	2	2
GO:0060285	cilium-dependent cell motility	4	2
GO:0061640	cytoskeleton-dependent cytokinesis	4	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:1902850	microtubule cytoskeleton organization involved in mitosis	4	2
GO:1903047	mitotic cell cycle process	3	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	7
GO:0005509	calcium ion binding	5	7
GO:0005515	protein binding	2	2
GO:0008092	cytoskeletal protein binding	3	2
GO:0015631	tubulin binding	4	2
GO:0043014	alpha-tubulin binding	5	2
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	723	729	PF00089	0.499
CLV_NRD_NRD_1	115	117	PF00675	0.255
CLV_NRD_NRD_1	251	253	PF00675	0.334
CLV_NRD_NRD_1	29	31	PF00675	0.534
CLV_NRD_NRD_1	34	36	PF00675	0.537
CLV_NRD_NRD_1	396	398	PF00675	0.574
CLV_NRD_NRD_1	416	418	PF00675	0.477
CLV_NRD_NRD_1	441	443	PF00675	0.255
CLV_NRD_NRD_1	478	480	PF00675	0.307
CLV_NRD_NRD_1	532	534	PF00675	0.412
CLV_NRD_NRD_1	646	648	PF00675	0.651
CLV_NRD_NRD_1	669	671	PF00675	0.404
CLV_NRD_NRD_1	699	701	PF00675	0.458
CLV_PCSK_KEX2_1	148	150	PF00082	0.255
CLV_PCSK_KEX2_1	29	31	PF00082	0.534
CLV_PCSK_KEX2_1	34	36	PF00082	0.537
CLV_PCSK_KEX2_1	396	398	PF00082	0.574
CLV_PCSK_KEX2_1	416	418	PF00082	0.512
CLV_PCSK_KEX2_1	441	443	PF00082	0.255
CLV_PCSK_KEX2_1	478	480	PF00082	0.255
CLV_PCSK_KEX2_1	669	671	PF00082	0.380
CLV_PCSK_KEX2_1	699	701	PF00082	0.547
CLV_PCSK_PC1ET2_1	148	150	PF00082	0.255
CLV_PCSK_PC7_1	30	36	PF00082	0.655
CLV_PCSK_PC7_1	474	480	PF00082	0.255
CLV_PCSK_SKI1_1	117	121	PF00082	0.238
CLV_PCSK_SKI1_1	188	192	PF00082	0.436
CLV_PCSK_SKI1_1	245	249	PF00082	0.346
CLV_PCSK_SKI1_1	252	256	PF00082	0.314
CLV_PCSK_SKI1_1	271	275	PF00082	0.152
CLV_PCSK_SKI1_1	308	312	PF00082	0.238
CLV_PCSK_SKI1_1	354	358	PF00082	0.248
CLV_PCSK_SKI1_1	442	446	PF00082	0.249
CLV_PCSK_SKI1_1	495	499	PF00082	0.303
CLV_PCSK_SKI1_1	55	59	PF00082	0.669
CLV_PCSK_SKI1_1	670	674	PF00082	0.424
CLV_PCSK_SKI1_1	717	721	PF00082	0.476
CLV_Separin_Metazoa	26	30	PF03568	0.553
DEG_APCC_KENBOX_2	329	333	PF00400	0.509
DEG_SIAH_1	198	206	PF03145	0.598
DOC_ANK_TNKS_1	604	611	PF00023	0.487
DOC_CDC14_PxL_1	15	23	PF14671	0.664
DOC_CYCLIN_RxL_1	185	193	PF00134	0.637
DOC_CYCLIN_RxL_1	568	579	PF00134	0.380
DOC_CYCLIN_RxL_1	664	676	PF00134	0.410
DOC_CYCLIN_RxL_1	695	708	PF00134	0.520
DOC_MAPK_FxFP_2	141	144	PF00069	0.452
DOC_MAPK_gen_1	114	122	PF00069	0.457
DOC_MAPK_gen_1	242	250	PF00069	0.452
DOC_MAPK_gen_1	34	42	PF00069	0.671
DOC_MAPK_gen_1	439	449	PF00069	0.454
DOC_MAPK_MEF2A_6	114	122	PF00069	0.507
DOC_MAPK_MEF2A_6	441	449	PF00069	0.525
DOC_PP4_FxxP_1	13	16	PF00568	0.535
DOC_PP4_FxxP_1	141	144	PF00568	0.452
DOC_PP4_FxxP_1	389	392	PF00568	0.595
DOC_USP7_MATH_1	450	454	PF00917	0.497
DOC_USP7_MATH_1	612	616	PF00917	0.531
DOC_USP7_MATH_1	631	635	PF00917	0.670
DOC_USP7_MATH_1	653	657	PF00917	0.469
DOC_USP7_MATH_1	684	688	PF00917	0.541
DOC_USP7_MATH_1	725	729	PF00917	0.531
DOC_USP7_MATH_1	748	752	PF00917	0.542
DOC_USP7_UBL2_3	435	439	PF12436	0.544
DOC_USP7_UBL2_3	530	534	PF12436	0.544
DOC_WW_Pin1_4	155	160	PF00397	0.451
DOC_WW_Pin1_4	227	232	PF00397	0.544
DOC_WW_Pin1_4	234	239	PF00397	0.502
DOC_WW_Pin1_4	382	387	PF00397	0.584
LIG_14-3-3_CanoR_1	419	425	PF00244	0.380
LIG_14-3-3_CanoR_1	726	730	PF00244	0.432
LIG_AP2alpha_2	184	186	PF02296	0.558
LIG_AP2alpha_2	298	300	PF02296	0.455
LIG_BIR_II_1	1	5	PF00653	0.565
LIG_BIR_III_2	182	186	PF00653	0.558
LIG_BRCT_BRCA1_1	169	173	PF00533	0.475
LIG_BRCT_BRCA1_1	283	287	PF00533	0.563
LIG_BRCT_BRCA1_1	315	319	PF00533	0.528
LIG_BRCT_BRCA1_1	325	329	PF00533	0.468
LIG_BRCT_BRCA1_1	499	503	PF00533	0.534
LIG_BRCT_BRCA1_1	690	694	PF00533	0.486
LIG_eIF4E_1	683	689	PF01652	0.536
LIG_FHA_1	110	116	PF00498	0.504
LIG_FHA_1	126	132	PF00498	0.453
LIG_FHA_1	175	181	PF00498	0.455
LIG_FHA_1	185	191	PF00498	0.523
LIG_FHA_1	262	268	PF00498	0.463
LIG_FHA_1	541	547	PF00498	0.432
LIG_FHA_1	89	95	PF00498	0.356
LIG_FHA_2	258	264	PF00498	0.534
LIG_FHA_2	326	332	PF00498	0.462
LIG_FHA_2	337	343	PF00498	0.441
LIG_FHA_2	393	399	PF00498	0.603
LIG_FHA_2	401	407	PF00498	0.509
LIG_FHA_2	576	582	PF00498	0.419
LIG_LIR_Apic_2	12	16	PF02991	0.541
LIG_LIR_Apic_2	139	144	PF02991	0.452
LIG_LIR_Gen_1	206	217	PF02991	0.503
LIG_LIR_Gen_1	270	280	PF02991	0.522
LIG_LIR_Gen_1	500	510	PF02991	0.489
LIG_LIR_Gen_1	564	573	PF02991	0.284
LIG_LIR_Gen_1	598	607	PF02991	0.463
LIG_LIR_Gen_1	661	666	PF02991	0.441
LIG_LIR_Gen_1	676	684	PF02991	0.376
LIG_LIR_Gen_1	728	736	PF02991	0.490
LIG_LIR_Nem_3	177	181	PF02991	0.511
LIG_LIR_Nem_3	270	276	PF02991	0.474
LIG_LIR_Nem_3	298	303	PF02991	0.444
LIG_LIR_Nem_3	360	364	PF02991	0.463
LIG_LIR_Nem_3	455	460	PF02991	0.485
LIG_LIR_Nem_3	500	506	PF02991	0.489
LIG_LIR_Nem_3	564	569	PF02991	0.269
LIG_LIR_Nem_3	594	599	PF02991	0.308
LIG_LIR_Nem_3	661	665	PF02991	0.433
LIG_LIR_Nem_3	676	680	PF02991	0.289
LIG_LIR_Nem_3	728	732	PF02991	0.496
LIG_Pex14_1	620	624	PF04695	0.540
LIG_Pex14_2	673	677	PF04695	0.447
LIG_SH2_NCK_1	599	603	PF00017	0.419
LIG_SH2_SRC	599	602	PF00017	0.419
LIG_SH2_SRC	624	627	PF00017	0.601
LIG_SH2_STAP1	599	603	PF00017	0.308
LIG_SH2_STAP1	729	733	PF00017	0.483
LIG_SH2_STAP1	742	746	PF00017	0.532
LIG_SH2_STAT3	352	355	PF00017	0.558
LIG_SH2_STAT5	100	103	PF00017	0.468
LIG_SH2_STAT5	123	126	PF00017	0.507
LIG_SH2_STAT5	209	212	PF00017	0.530
LIG_SH2_STAT5	278	281	PF00017	0.460
LIG_SH2_STAT5	338	341	PF00017	0.455
LIG_SH2_STAT5	364	367	PF00017	0.556
LIG_SH2_STAT5	448	451	PF00017	0.535
LIG_SH2_STAT5	484	487	PF00017	0.470
LIG_SH2_STAT5	491	494	PF00017	0.466
LIG_SH2_STAT5	588	591	PF00017	0.308
LIG_SH2_STAT5	596	599	PF00017	0.308
LIG_SH3_3	128	134	PF00018	0.534
LIG_SH3_3	13	19	PF00018	0.533
LIG_SH3_3	149	155	PF00018	0.507
LIG_SH3_3	385	391	PF00018	0.492
LIG_SUMO_SIM_anti_2	581	587	PF11976	0.327
LIG_TRAF2_1	194	197	PF00917	0.455
LIG_TRAF2_1	578	581	PF00917	0.308
LIG_TRAF2_1	598	601	PF00917	0.122
LIG_TRAF2_1	708	711	PF00917	0.508
LIG_TRAF2_1	728	731	PF00917	0.295
LIG_TYR_ITIM	597	602	PF00017	0.406
LIG_UBA3_1	582	591	PF00899	0.319
MOD_CK1_1	281	287	PF00069	0.503
MOD_CK1_1	323	329	PF00069	0.525
MOD_CK1_1	400	406	PF00069	0.587
MOD_CK1_1	541	547	PF00069	0.414
MOD_CK1_1	595	601	PF00069	0.308
MOD_CK1_1	705	711	PF00069	0.491
MOD_CK2_1	105	111	PF00069	0.457
MOD_CK2_1	127	133	PF00069	0.450
MOD_CK2_1	19	25	PF00069	0.530
MOD_CK2_1	325	331	PF00069	0.485
MOD_CK2_1	392	398	PF00069	0.590
MOD_CK2_1	400	406	PF00069	0.490
MOD_CK2_1	452	458	PF00069	0.485
MOD_CK2_1	575	581	PF00069	0.308
MOD_CK2_1	595	601	PF00069	0.122
MOD_CK2_1	705	711	PF00069	0.435
MOD_CK2_1	725	731	PF00069	0.344
MOD_GlcNHglycan	137	141	PF01048	0.250
MOD_GlcNHglycan	294	297	PF01048	0.245
MOD_GlcNHglycan	313	316	PF01048	0.122
MOD_GlcNHglycan	463	466	PF01048	0.255
MOD_GlcNHglycan	557	560	PF01048	0.363
MOD_GSK3_1	105	112	PF00069	0.531
MOD_GSK3_1	125	132	PF00069	0.475
MOD_GSK3_1	203	210	PF00069	0.511
MOD_GSK3_1	222	229	PF00069	0.605
MOD_GSK3_1	257	264	PF00069	0.514
MOD_GSK3_1	319	326	PF00069	0.500
MOD_GSK3_1	332	339	PF00069	0.468
MOD_GSK3_1	505	512	PF00069	0.444
MOD_GSK3_1	55	62	PF00069	0.667
MOD_GSK3_1	684	691	PF00069	0.461
MOD_GSK3_1	738	745	PF00069	0.532
MOD_GSK3_1	748	755	PF00069	0.571
MOD_N-GLC_1	555	560	PF02516	0.400
MOD_N-GLC_1	592	597	PF02516	0.316
MOD_N-GLC_1	736	741	PF02516	0.411
MOD_NEK2_1	267	272	PF00069	0.501
MOD_NEK2_1	319	324	PF00069	0.527
MOD_NEK2_1	373	378	PF00069	0.492
MOD_NEK2_1	420	425	PF00069	0.517
MOD_NEK2_1	538	543	PF00069	0.402
MOD_NEK2_1	702	707	PF00069	0.485
MOD_NEK2_1	736	741	PF00069	0.429
MOD_NEK2_2	208	213	PF00069	0.549
MOD_NEK2_2	325	330	PF00069	0.485
MOD_NEK2_2	65	70	PF00069	0.737
MOD_PIKK_1	3	9	PF00454	0.553
MOD_PKA_2	461	467	PF00069	0.468
MOD_PKA_2	646	652	PF00069	0.622
MOD_PKA_2	725	731	PF00069	0.433
MOD_Plk_1	257	263	PF00069	0.534
MOD_Plk_1	319	325	PF00069	0.534
MOD_Plk_1	400	406	PF00069	0.587
MOD_Plk_1	450	456	PF00069	0.446
MOD_Plk_1	509	515	PF00069	0.530
MOD_Plk_1	53	59	PF00069	0.662
MOD_Plk_1	592	598	PF00069	0.297
MOD_Plk_1	702	708	PF00069	0.509
MOD_Plk_2-3	703	709	PF00069	0.486
MOD_Plk_4	105	111	PF00069	0.478
MOD_Plk_4	169	175	PF00069	0.534
MOD_Plk_4	231	237	PF00069	0.620
MOD_Plk_4	263	269	PF00069	0.515
MOD_Plk_4	272	278	PF00069	0.409
MOD_Plk_4	332	338	PF00069	0.468
MOD_Plk_4	420	426	PF00069	0.534
MOD_Plk_4	452	458	PF00069	0.537
MOD_Plk_4	565	571	PF00069	0.352
MOD_Plk_4	581	587	PF00069	0.209
MOD_Plk_4	592	598	PF00069	0.308
MOD_Plk_4	631	637	PF00069	0.650
MOD_Plk_4	684	690	PF00069	0.428
MOD_ProDKin_1	155	161	PF00069	0.451
MOD_ProDKin_1	227	233	PF00069	0.539
MOD_ProDKin_1	234	240	PF00069	0.497
MOD_ProDKin_1	382	388	PF00069	0.592
MOD_SUMO_rev_2	249	255	PF00179	0.534
MOD_SUMO_rev_2	432	440	PF00179	0.558
MOD_SUMO_rev_2	634	642	PF00179	0.580
MOD_SUMO_rev_2	643	649	PF00179	0.505
MOD_SUMO_rev_2	687	696	PF00179	0.437
TRG_DiLeu_BaEn_1	731	736	PF01217	0.482
TRG_DiLeu_BaEn_4	710	716	PF01217	0.487
TRG_DiLeu_LyEn_5	731	736	PF01217	0.514
TRG_ENDOCYTIC_2	209	212	PF00928	0.499
TRG_ENDOCYTIC_2	277	280	PF00928	0.460
TRG_ENDOCYTIC_2	599	602	PF00928	0.308
TRG_ENDOCYTIC_2	729	732	PF00928	0.453
TRG_ER_diArg_1	212	215	PF00400	0.537
TRG_ER_diArg_1	28	30	PF00400	0.537
TRG_ER_diArg_1	306	309	PF00400	0.460
TRG_ER_diArg_1	415	417	PF00400	0.533
TRG_ER_diArg_1	440	442	PF00400	0.455
TRG_ER_diArg_1	477	479	PF00400	0.508
TRG_Pf-PMV_PEXEL_1	188	193	PF00026	0.538
TRG_Pf-PMV_PEXEL_1	397	401	PF00026	0.658

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5H4	Leptomonas seymouri	23%	90%
A0A0N1HZ98	Leptomonas seymouri	25%	98%
A0A0N1I3M2	Leptomonas seymouri	76%	99%
A0A0N1PBW1	Leptomonas seymouri	28%	100%
A0A0S4IJW8	Bodo saltans	26%	92%
A0A0S4IQM0	Bodo saltans	25%	100%
A0A0S4J8J7	Bodo saltans	27%	82%
A0A1X0NEV8	Trypanosomatidae	25%	95%
A0A1X0NLI2	Trypanosomatidae	27%	100%
A0A1X0NMQ3	Trypanosomatidae	25%	97%
A0A1X0P419	Trypanosomatidae	62%	99%
A0A3Q1N1R0	Bos taurus	30%	100%
A0A3Q8IDH4	Leishmania donovani	25%	98%
A0A3Q8IPU3	Leishmania donovani	97%	100%
A0A3R7KM50	Trypanosoma rangeli	27%	95%
A0A3R7L048	Trypanosoma rangeli	59%	100%
A0A3S5H688	Leishmania donovani	22%	87%
A0A3S7XC76	Leishmania donovani	27%	100%
A0A422N0Z6	Trypanosoma rangeli	24%	97%
A0A422NK00	Trypanosoma rangeli	29%	100%
A4HE78	Leishmania braziliensis	26%	98%
A4HFK9	Leishmania braziliensis	88%	100%
A4HQF8	Leishmania braziliensis	26%	100%
A4I1J2	Leishmania infantum	25%	98%
A4IC94	Leishmania infantum	27%	100%
C9ZJX4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	94%
C9ZPE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	97%
D0A3K7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	100%
D0A5T5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	100%
E1BKH1	Bos taurus	29%	100%
E9AHF6	Leishmania infantum	98%	100%
E9AI34	Leishmania braziliensis	23%	88%
E9AMI2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	88%
E9AU74	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9AXM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	98%
E9AYY9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
Q32TF8	Danio rerio	31%	100%
Q32TG3	Gallus gallus	28%	100%
Q4Q0C0	Leishmania major	27%	100%
Q4Q9U5	Leishmania major	25%	98%
Q4QI57	Leishmania major	23%	87%
Q5JST6	Homo sapiens	28%	100%
Q5JVL4	Homo sapiens	30%	100%
Q9D485	Mus musculus	29%	100%
Q9D9T8	Mus musculus	30%	100%
V5ASV2	Trypanosoma cruzi	26%	100%
V5D3X9	Trypanosoma cruzi	24%	97%
V5DA42	Trypanosoma cruzi	59%	100%