Calcium uniporter protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calcium uniporter protein, mitochondrial

Species:

Leishmania major

UniProt:

E9AD55_LEIMA

TriTrypDb:

LmjF.27.0780 , LMJLV39_270013500 , LMJSD75_270013500

Length:

319

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0016020	membrane	2	12
GO:0031090	organelle membrane	3	12
GO:0031966	mitochondrial membrane	4	12
GO:0032991	protein-containing complex	1	2
GO:0034702	monoatomic ion channel complex	4	2
GO:0034703	cation channel complex	5	2
GO:0034704	calcium channel complex	6	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0098796	membrane protein complex	2	2
GO:0098798	mitochondrial protein-containing complex	2	2
GO:0098800	inner mitochondrial membrane protein complex	3	2
GO:0110165	cellular anatomical entity	1	12
GO:1902495	transmembrane transporter complex	3	2
GO:1990246	uniplex complex	4	2
GO:1990351	transporter complex	2	2
GO:0005743	mitochondrial inner membrane	5	10
GO:0019866	organelle inner membrane	4	10

Expansion

Sequence features

E9AD55

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AD55

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006811	monoatomic ion transport	4	2
GO:0006812	monoatomic cation transport	5	2
GO:0006816	calcium ion transport	7	2
GO:0006851	mitochondrial calcium ion transmembrane transport	4	2
GO:0006873	intracellular monoatomic ion homeostasis	4	12
GO:0006874	intracellular calcium ion homeostasis	7	12
GO:0006875	obsolete intracellular metal ion homeostasis	6	12
GO:0009987	cellular process	1	12
GO:0019725	cellular homeostasis	2	12
GO:0030001	metal ion transport	6	2
GO:0030003	intracellular monoatomic cation homeostasis	5	12
GO:0034220	monoatomic ion transmembrane transport	3	2
GO:0036444	calcium import into the mitochondrion	5	2
GO:0042592	homeostatic process	3	12
GO:0048878	chemical homeostasis	4	12
GO:0050801	monoatomic ion homeostasis	5	12
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051560	mitochondrial calcium ion homeostasis	8	12
GO:0055065	obsolete metal ion homeostasis	7	12
GO:0055074	calcium ion homeostasis	8	12
GO:0055080	monoatomic cation homeostasis	6	12
GO:0055082	intracellular chemical homeostasis	3	12
GO:0055085	transmembrane transport	2	2
GO:0065007	biological regulation	1	12
GO:0065008	regulation of biological quality	2	12
GO:0070588	calcium ion transmembrane transport	6	2
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	12
GO:0072507	obsolete divalent inorganic cation homeostasis	7	12
GO:0098655	monoatomic cation transmembrane transport	4	2
GO:0098660	inorganic ion transmembrane transport	4	2
GO:0098662	inorganic cation transmembrane transport	5	2
GO:0098771	inorganic ion homeostasis	6	12
GO:1990542	mitochondrial transmembrane transport	3	2

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	12
GO:0005216	monoatomic ion channel activity	4	12
GO:0005261	monoatomic cation channel activity	5	12
GO:0005262	calcium channel activity	6	12
GO:0008324	monoatomic cation transmembrane transporter activity	4	12
GO:0015075	monoatomic ion transmembrane transporter activity	3	12
GO:0015085	calcium ion transmembrane transporter activity	6	12
GO:0015267	channel activity	4	12
GO:0015291	secondary active transmembrane transporter activity	4	12
GO:0015292	uniporter activity	5	12
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	12
GO:0022803	passive transmembrane transporter activity	3	12
GO:0022804	active transmembrane transporter activity	3	12
GO:0022857	transmembrane transporter activity	2	12
GO:0022890	inorganic cation transmembrane transporter activity	4	12
GO:0046873	metal ion transmembrane transporter activity	5	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	158	162	PF00656	0.479
CLV_C14_Caspase3-7	77	81	PF00656	0.652
CLV_NRD_NRD_1	10	12	PF00675	0.488
CLV_NRD_NRD_1	184	186	PF00675	0.275
CLV_NRD_NRD_1	211	213	PF00675	0.204
CLV_NRD_NRD_1	279	281	PF00675	0.249
CLV_NRD_NRD_1	30	32	PF00675	0.425
CLV_PCSK_FUR_1	209	213	PF00082	0.311
CLV_PCSK_KEX2_1	10	12	PF00082	0.488
CLV_PCSK_KEX2_1	184	186	PF00082	0.250
CLV_PCSK_KEX2_1	211	213	PF00082	0.202
CLV_PCSK_KEX2_1	29	31	PF00082	0.426
CLV_PCSK_SKI1_1	139	143	PF00082	0.311
CLV_PCSK_SKI1_1	306	310	PF00082	0.328
CLV_PCSK_SKI1_1	70	74	PF00082	0.332
DEG_APCC_DBOX_1	28	36	PF00400	0.587
DEG_APCC_DBOX_1	305	313	PF00400	0.503
DEG_Nend_UBRbox_3	1	3	PF02207	0.605
DEG_SPOP_SBC_1	19	23	PF00917	0.611
DOC_CYCLIN_yClb1_LxF_4	252	258	PF00134	0.285
DOC_PP1_RVXF_1	137	144	PF00149	0.401
DOC_USP7_MATH_1	154	158	PF00917	0.487
DOC_USP7_MATH_1	173	177	PF00917	0.530
DOC_USP7_MATH_1	45	49	PF00917	0.647
DOC_USP7_MATH_1	50	54	PF00917	0.638
DOC_USP7_UBL2_3	314	318	PF12436	0.586
DOC_WW_Pin1_4	61	66	PF00397	0.624
LIG_14-3-3_CanoR_1	155	164	PF00244	0.367
LIG_14-3-3_CanoR_1	209	215	PF00244	0.516
LIG_14-3-3_CanoR_1	231	235	PF00244	0.257
LIG_14-3-3_CanoR_1	29	39	PF00244	0.649
LIG_Actin_WH2_2	123	141	PF00022	0.463
LIG_APCC_ABBAyCdc20_2	139	145	PF00400	0.511
LIG_FHA_1	20	26	PF00498	0.704
LIG_FHA_1	62	68	PF00498	0.638
LIG_FHA_2	188	194	PF00498	0.559
LIG_FHA_2	231	237	PF00498	0.242
LIG_FHA_2	269	275	PF00498	0.429
LIG_FHA_2	75	81	PF00498	0.604
LIG_GBD_Chelix_1	251	259	PF00786	0.241
LIG_HCF-1_HBM_1	267	270	PF13415	0.475
LIG_LIR_Gen_1	100	108	PF02991	0.589
LIG_LIR_Gen_1	236	247	PF02991	0.242
LIG_LIR_Gen_1	59	68	PF02991	0.533
LIG_LIR_Nem_3	100	105	PF02991	0.550
LIG_LIR_Nem_3	233	237	PF02991	0.235
LIG_LIR_Nem_3	254	260	PF02991	0.238
LIG_LIR_Nem_3	267	273	PF02991	0.415
LIG_LIR_Nem_3	289	295	PF02991	0.421
LIG_LIR_Nem_3	59	63	PF02991	0.543
LIG_LYPXL_SIV_4	294	302	PF13949	0.471
LIG_Pex14_2	235	239	PF04695	0.232
LIG_SH2_SRC	295	298	PF00017	0.454
LIG_SH2_STAP1	273	277	PF00017	0.381
LIG_SH2_STAT3	198	201	PF00017	0.360
LIG_SH2_STAT5	234	237	PF00017	0.247
LIG_SH2_STAT5	261	264	PF00017	0.395
LIG_SH2_STAT5	270	273	PF00017	0.412
LIG_SH3_3	108	114	PF00018	0.579
LIG_SH3_3	150	156	PF00018	0.472
LIG_SUMO_SIM_anti_2	64	70	PF11976	0.552
LIG_SUMO_SIM_par_1	125	131	PF11976	0.440
LIG_SUMO_SIM_par_1	170	176	PF11976	0.360
LIG_TRFH_1	198	202	PF08558	0.360
LIG_TYR_ITIM	232	237	PF00017	0.301
LIG_UBA3_1	308	314	PF00899	0.587
LIG_WRC_WIRS_1	174	179	PF05994	0.498
MOD_CK1_1	18	24	PF00069	0.709
MOD_CK1_1	230	236	PF00069	0.251
MOD_CK1_1	53	59	PF00069	0.577
MOD_CK2_1	187	193	PF00069	0.510
MOD_GlcNHglycan	11	14	PF01048	0.425
MOD_GlcNHglycan	158	161	PF01048	0.352
MOD_GlcNHglycan	17	20	PF01048	0.375
MOD_GlcNHglycan	315	318	PF01048	0.334
MOD_GlcNHglycan	4	7	PF01048	0.456
MOD_GlcNHglycan	47	50	PF01048	0.446
MOD_GlcNHglycan	52	55	PF01048	0.444
MOD_GSK3_1	15	22	PF00069	0.673
MOD_GSK3_1	156	163	PF00069	0.467
MOD_GSK3_1	243	250	PF00069	0.340
MOD_GSK3_1	45	52	PF00069	0.616
MOD_NEK2_1	164	169	PF00069	0.360
MOD_NEK2_1	308	313	PF00069	0.570
MOD_NEK2_1	4	9	PF00069	0.711
MOD_PIKK_1	74	80	PF00454	0.658
MOD_PKA_1	30	36	PF00069	0.738
MOD_PKA_2	154	160	PF00069	0.423
MOD_PKA_2	210	216	PF00069	0.475
MOD_PKA_2	230	236	PF00069	0.121
MOD_PKA_2	30	36	PF00069	0.664
MOD_PKA_2	9	15	PF00069	0.733
MOD_PKB_1	29	37	PF00069	0.586
MOD_Plk_1	266	272	PF00069	0.433
MOD_Plk_4	173	179	PF00069	0.414
MOD_Plk_4	230	236	PF00069	0.257
MOD_Plk_4	243	249	PF00069	0.319
MOD_Plk_4	251	257	PF00069	0.327
MOD_Plk_4	308	314	PF00069	0.451
MOD_Plk_4	4	10	PF00069	0.638
MOD_ProDKin_1	61	67	PF00069	0.625
TRG_DiLeu_BaEn_4	304	310	PF01217	0.491
TRG_DiLeu_BaLyEn_6	62	67	PF01217	0.607
TRG_ENDOCYTIC_2	234	237	PF00928	0.284
TRG_ENDOCYTIC_2	261	264	PF00928	0.412
TRG_ENDOCYTIC_2	295	298	PF00928	0.418
TRG_ER_diArg_1	184	186	PF00400	0.475
TRG_ER_diArg_1	210	212	PF00400	0.396
TRG_ER_diArg_1	28	31	PF00400	0.632
TRG_ER_diArg_1	9	11	PF00400	0.741
TRG_NLS_MonoExtN_4	279	284	PF00514	0.475

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8M7	Leptomonas seymouri	58%	96%
A0A0S4JRI1	Bodo saltans	37%	100%
A0A1X0P3Z9	Trypanosomatidae	38%	100%
A0A3Q8IHI0	Leishmania donovani	90%	100%
A0A422NMN9	Trypanosoma rangeli	43%	100%
A4HFK2	Leishmania braziliensis	72%	100%
D0A5S5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
E9AHF0	Leishmania infantum	90%	100%
E9AYY0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
Q810S1	Mus musculus	25%	92%
Q9NWR8	Homo sapiens	24%	95%
V5DA35	Trypanosoma cruzi	45%	100%