Uncharacterized protein

Quick info Localization Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Nucleolar protein 60, putative

Species:

Leishmania major

UniProt:

E9AD25_LEIMA

TriTrypDb:

LmjF.27.0480 , LMJLV39_270009800 , LMJSD75_270009800

Length:

595

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AD25

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AD25

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	33	37	PF00656	0.526
CLV_C14_Caspase3-7	355	359	PF00656	0.666
CLV_C14_Caspase3-7	40	44	PF00656	0.379
CLV_NRD_NRD_1	172	174	PF00675	0.578
CLV_NRD_NRD_1	196	198	PF00675	0.641
CLV_NRD_NRD_1	273	275	PF00675	0.458
CLV_NRD_NRD_1	487	489	PF00675	0.634
CLV_NRD_NRD_1	519	521	PF00675	0.574
CLV_PCSK_FUR_1	271	275	PF00082	0.509
CLV_PCSK_KEX2_1	172	174	PF00082	0.578
CLV_PCSK_KEX2_1	196	198	PF00082	0.621
CLV_PCSK_KEX2_1	273	275	PF00082	0.473
CLV_PCSK_KEX2_1	487	489	PF00082	0.660
CLV_PCSK_KEX2_1	519	521	PF00082	0.576
CLV_PCSK_SKI1_1	104	108	PF00082	0.484
CLV_PCSK_SKI1_1	120	124	PF00082	0.544
CLV_PCSK_SKI1_1	470	474	PF00082	0.584
CLV_Separin_Metazoa	536	540	PF03568	0.710
DOC_CKS1_1	401	406	PF01111	0.572
DOC_CYCLIN_yCln2_LP_2	108	114	PF00134	0.499
DOC_CYCLIN_yCln2_LP_2	443	449	PF00134	0.642
DOC_CYCLIN_yCln2_LP_2	54	60	PF00134	0.531
DOC_MAPK_gen_1	22	30	PF00069	0.481
DOC_MAPK_gen_1	539	549	PF00069	0.660
DOC_MAPK_gen_1	568	574	PF00069	0.628
DOC_MAPK_MEF2A_6	22	30	PF00069	0.472
DOC_PP2B_LxvP_1	108	111	PF13499	0.470
DOC_PP2B_LxvP_1	112	115	PF13499	0.486
DOC_PP2B_LxvP_1	443	446	PF13499	0.644
DOC_PP2B_LxvP_1	54	57	PF13499	0.498
DOC_PP4_FxxP_1	401	404	PF00568	0.536
DOC_PP4_FxxP_1	476	479	PF00568	0.553
DOC_USP7_MATH_1	218	222	PF00917	0.594
DOC_USP7_MATH_1	4	8	PF00917	0.498
DOC_USP7_MATH_1	417	421	PF00917	0.508
DOC_USP7_MATH_1	452	456	PF00917	0.431
DOC_USP7_MATH_1	472	476	PF00917	0.506
DOC_USP7_MATH_1	586	590	PF00917	0.600
DOC_WW_Pin1_4	133	138	PF00397	0.642
DOC_WW_Pin1_4	154	159	PF00397	0.536
DOC_WW_Pin1_4	213	218	PF00397	0.656
DOC_WW_Pin1_4	373	378	PF00397	0.686
DOC_WW_Pin1_4	400	405	PF00397	0.566
LIG_14-3-3_CanoR_1	22	27	PF00244	0.507
LIG_14-3-3_CanoR_1	297	301	PF00244	0.543
LIG_14-3-3_CanoR_1	470	479	PF00244	0.565
LIG_14-3-3_CanoR_1	539	549	PF00244	0.604
LIG_14-3-3_CanoR_1	89	95	PF00244	0.549
LIG_Actin_WH2_2	552	570	PF00022	0.481
LIG_AP2alpha_1	63	67	PF02296	0.586
LIG_AP2alpha_2	300	302	PF02296	0.551
LIG_BIR_II_1	1	5	PF00653	0.464
LIG_BIR_III_4	125	129	PF00653	0.357
LIG_BRCT_BRCA1_1	220	224	PF00533	0.446
LIG_BRCT_BRCA1_1	454	458	PF00533	0.548
LIG_FHA_1	151	157	PF00498	0.482
LIG_FHA_1	23	29	PF00498	0.428
LIG_FHA_1	242	248	PF00498	0.608
LIG_FHA_1	267	273	PF00498	0.559
LIG_FHA_1	322	328	PF00498	0.530
LIG_FHA_1	438	444	PF00498	0.563
LIG_FHA_1	549	555	PF00498	0.699
LIG_FHA_1	69	75	PF00498	0.594
LIG_FHA_2	326	332	PF00498	0.475
LIG_GBD_Chelix_1	26	34	PF00786	0.289
LIG_LIR_Apic_2	398	404	PF02991	0.567
LIG_LIR_Apic_2	473	479	PF02991	0.467
LIG_LIR_Gen_1	305	314	PF02991	0.390
LIG_LIR_Gen_1	36	42	PF02991	0.412
LIG_LIR_Nem_3	163	169	PF02991	0.493
LIG_LIR_Nem_3	305	310	PF02991	0.390
LIG_LIR_Nem_3	36	41	PF02991	0.409
LIG_LIR_Nem_3	400	405	PF02991	0.501
LIG_PDZ_Class_1	590	595	PF00595	0.629
LIG_Pex14_2	63	67	PF04695	0.586
LIG_SH2_CRK	402	406	PF00017	0.466
LIG_SH2_CRK	466	470	PF00017	0.518
LIG_SH2_PTP2	307	310	PF00017	0.344
LIG_SH2_SRC	261	264	PF00017	0.553
LIG_SH2_SRC	279	282	PF00017	0.556
LIG_SH2_SRC	311	314	PF00017	0.526
LIG_SH2_STAP1	279	283	PF00017	0.548
LIG_SH2_STAP1	68	72	PF00017	0.589
LIG_SH2_STAT3	180	183	PF00017	0.583
LIG_SH2_STAT5	180	183	PF00017	0.590
LIG_SH2_STAT5	307	310	PF00017	0.372
LIG_SH2_STAT5	311	314	PF00017	0.439
LIG_SH2_STAT5	329	332	PF00017	0.355
LIG_SH2_STAT5	335	338	PF00017	0.454
LIG_SH2_STAT5	402	405	PF00017	0.432
LIG_SH2_STAT5	49	52	PF00017	0.365
LIG_SH2_STAT5	68	71	PF00017	0.590
LIG_SH3_2	263	268	PF14604	0.549
LIG_SH3_3	257	263	PF00018	0.446
LIG_SH3_3	307	313	PF00018	0.495
LIG_SH3_3	362	368	PF00018	0.562
LIG_SH3_3	436	442	PF00018	0.575
LIG_SH3_3	443	449	PF00018	0.627
LIG_SH3_3	80	86	PF00018	0.523
LIG_SUMO_SIM_par_1	25	31	PF11976	0.413
LIG_SUMO_SIM_par_1	348	353	PF11976	0.498
LIG_SUMO_SIM_par_1	409	415	PF11976	0.498
LIG_SUMO_SIM_par_1	550	558	PF11976	0.469
LIG_TRAF2_1	313	316	PF00917	0.542
LIG_TYR_ITSM	398	405	PF00017	0.490
MOD_CDC14_SPxK_1	376	379	PF00782	0.673
MOD_CDK_SPxK_1	373	379	PF00069	0.675
MOD_CDK_SPxK_1	400	406	PF00069	0.575
MOD_CK1_1	100	106	PF00069	0.497
MOD_CK1_1	208	214	PF00069	0.742
MOD_CK1_1	216	222	PF00069	0.672
MOD_CK1_1	340	346	PF00069	0.500
MOD_CK1_1	37	43	PF00069	0.643
MOD_CK1_1	412	418	PF00069	0.461
MOD_CK1_1	522	528	PF00069	0.745
MOD_CK1_1	552	558	PF00069	0.613
MOD_CK1_1	589	595	PF00069	0.607
MOD_CK2_1	148	154	PF00069	0.552
MOD_CK2_1	325	331	PF00069	0.480
MOD_Cter_Amidation	194	197	PF01082	0.726
MOD_GlcNHglycan	104	107	PF01048	0.480
MOD_GlcNHglycan	150	153	PF01048	0.502
MOD_GlcNHglycan	190	193	PF01048	0.770
MOD_GlcNHglycan	207	210	PF01048	0.500
MOD_GlcNHglycan	218	221	PF01048	0.633
MOD_GlcNHglycan	234	237	PF01048	0.337
MOD_GlcNHglycan	342	345	PF01048	0.543
MOD_GlcNHglycan	450	453	PF01048	0.559
MOD_GlcNHglycan	494	497	PF01048	0.731
MOD_GlcNHglycan	521	524	PF01048	0.746
MOD_GlcNHglycan	543	546	PF01048	0.626
MOD_GlcNHglycan	592	595	PF01048	0.780
MOD_GSK3_1	150	157	PF00069	0.464
MOD_GSK3_1	201	208	PF00069	0.727
MOD_GSK3_1	296	303	PF00069	0.621
MOD_GSK3_1	317	324	PF00069	0.457
MOD_GSK3_1	448	455	PF00069	0.526
MOD_GSK3_1	514	521	PF00069	0.689
MOD_GSK3_1	548	555	PF00069	0.591
MOD_GSK3_1	586	593	PF00069	0.619
MOD_N-GLC_1	470	475	PF02516	0.581
MOD_N-GLC_1	586	591	PF02516	0.675
MOD_N-GLC_2	370	372	PF02516	0.671
MOD_NEK2_1	34	39	PF00069	0.617
MOD_NEK2_1	419	424	PF00069	0.648
MOD_NEK2_2	266	271	PF00069	0.555
MOD_PIKK_1	452	458	PF00454	0.371
MOD_PKA_1	196	202	PF00069	0.722
MOD_PKA_1	519	525	PF00069	0.720
MOD_PKA_2	148	154	PF00069	0.496
MOD_PKA_2	195	201	PF00069	0.700
MOD_PKA_2	296	302	PF00069	0.545
MOD_PKA_2	378	384	PF00069	0.736
MOD_PKA_2	518	524	PF00069	0.712
MOD_PKA_2	541	547	PF00069	0.620
MOD_PKB_1	512	520	PF00069	0.618
MOD_Plk_1	266	272	PF00069	0.568
MOD_Plk_1	549	555	PF00069	0.629
MOD_Plk_4	113	119	PF00069	0.580
MOD_Plk_4	176	182	PF00069	0.496
MOD_Plk_4	218	224	PF00069	0.459
MOD_Plk_4	325	331	PF00069	0.512
MOD_Plk_4	397	403	PF00069	0.554
MOD_Plk_4	4	10	PF00069	0.458
MOD_Plk_4	419	425	PF00069	0.534
MOD_Plk_4	43	49	PF00069	0.521
MOD_Plk_4	464	470	PF00069	0.521
MOD_Plk_4	472	478	PF00069	0.577
MOD_Plk_4	549	555	PF00069	0.585
MOD_ProDKin_1	133	139	PF00069	0.622
MOD_ProDKin_1	154	160	PF00069	0.541
MOD_ProDKin_1	213	219	PF00069	0.654
MOD_ProDKin_1	373	379	PF00069	0.687
MOD_ProDKin_1	400	406	PF00069	0.575
MOD_SUMO_rev_2	221	231	PF00179	0.396
MOD_SUMO_rev_2	252	256	PF00179	0.564
MOD_SUMO_rev_2	353	361	PF00179	0.615
MOD_SUMO_rev_2	525	535	PF00179	0.764
TRG_DiLeu_BaEn_1	305	310	PF01217	0.371
TRG_DiLeu_BaLyEn_6	346	351	PF01217	0.562
TRG_DiLeu_LyEn_5	165	170	PF01217	0.596
TRG_ENDOCYTIC_2	257	260	PF00928	0.438
TRG_ENDOCYTIC_2	279	282	PF00928	0.556
TRG_ENDOCYTIC_2	307	310	PF00928	0.384
TRG_ENDOCYTIC_2	402	405	PF00928	0.466
TRG_ENDOCYTIC_2	466	469	PF00928	0.502
TRG_ER_diArg_1	172	174	PF00400	0.578
TRG_ER_diArg_1	21	24	PF00400	0.520
TRG_ER_diArg_1	270	273	PF00400	0.439
TRG_ER_diArg_1	382	385	PF00400	0.677
TRG_ER_diArg_1	486	488	PF00400	0.654
TRG_ER_diArg_1	491	494	PF00400	0.684

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I592	Leptomonas seymouri	53%	96%
A0A1X0P512	Trypanosomatidae	35%	100%
A0A3S7X0H0	Leishmania donovani	91%	99%
A0A422NTD4	Trypanosoma rangeli	37%	100%
A4HFH4	Leishmania braziliensis	74%	99%
A4I2N4	Leishmania infantum	91%	99%
D0A5Q0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9AYV0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
V5BSP9	Trypanosoma cruzi	33%	100%