Tubulin-tyrosine ligase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin-tyrosine ligase-like protein

Species:

Leishmania major

UniProt:

E9ACW9_LEIMA

TriTrypDb:

LmjF.27.2490 , LMJLV39_270032700 , LMJSD75_270032600

Length:

479

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005874	microtubule	6	2
GO:0005876	spindle microtubule	7	2
GO:0099080	supramolecular complex	2	2
GO:0099081	supramolecular polymer	3	2
GO:0099512	supramolecular fiber	4	2
GO:0099513	polymeric cytoskeletal fiber	5	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ACW9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACW9

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	2
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006996	organelle organization	4	2
GO:0007010	cytoskeleton organization	5	2
GO:0007017	microtubule-based process	2	2
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0019538	protein metabolic process	3	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0071840	cellular component organization or biogenesis	2	2
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004835	tubulin-tyrosine ligase activity	3	3
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	3
GO:0016881	acid-amino acid ligase activity	4	3
GO:0140096	catalytic activity, acting on a protein	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	157	159	PF00675	0.296
CLV_NRD_NRD_1	225	227	PF00675	0.276
CLV_NRD_NRD_1	31	33	PF00675	0.705
CLV_NRD_NRD_1	435	437	PF00675	0.757
CLV_NRD_NRD_1	466	468	PF00675	0.591
CLV_NRD_NRD_1	68	70	PF00675	0.536
CLV_PCSK_KEX2_1	157	159	PF00082	0.268
CLV_PCSK_KEX2_1	434	436	PF00082	0.682
CLV_PCSK_KEX2_1	466	468	PF00082	0.544
CLV_PCSK_KEX2_1	68	70	PF00082	0.543
CLV_PCSK_SKI1_1	157	161	PF00082	0.254
CLV_PCSK_SKI1_1	185	189	PF00082	0.293
CLV_PCSK_SKI1_1	218	222	PF00082	0.281
CLV_PCSK_SKI1_1	318	322	PF00082	0.286
CLV_PCSK_SKI1_1	323	327	PF00082	0.274
CLV_PCSK_SKI1_1	372	376	PF00082	0.483
CLV_PCSK_SKI1_1	382	386	PF00082	0.459
CLV_PCSK_SKI1_1	4	8	PF00082	0.621
DEG_APCC_DBOX_1	157	165	PF00400	0.539
DEG_APCC_DBOX_1	322	330	PF00400	0.493
DEG_Nend_UBRbox_1	1	4	PF02207	0.463
DOC_CKS1_1	373	378	PF01111	0.447
DOC_CYCLIN_RxL_1	312	324	PF00134	0.545
DOC_CYCLIN_yCln2_LP_2	236	242	PF00134	0.453
DOC_MAPK_gen_1	183	190	PF00069	0.495
DOC_MAPK_gen_1	226	232	PF00069	0.456
DOC_PP4_FxxP_1	373	376	PF00568	0.453
DOC_PP4_FxxP_1	86	89	PF00568	0.382
DOC_SPAK_OSR1_1	85	89	PF12202	0.376
DOC_USP7_MATH_1	203	207	PF00917	0.503
DOC_USP7_MATH_1	21	25	PF00917	0.628
DOC_USP7_MATH_1	34	38	PF00917	0.668
DOC_USP7_MATH_1	427	431	PF00917	0.625
DOC_USP7_MATH_1	443	447	PF00917	0.621
DOC_USP7_MATH_1	99	103	PF00917	0.421
DOC_WW_Pin1_4	10	15	PF00397	0.651
DOC_WW_Pin1_4	235	240	PF00397	0.453
DOC_WW_Pin1_4	24	29	PF00397	0.617
DOC_WW_Pin1_4	363	368	PF00397	0.446
DOC_WW_Pin1_4	372	377	PF00397	0.369
DOC_WW_Pin1_4	420	425	PF00397	0.768
LIG_14-3-3_CanoR_1	100	104	PF00244	0.332
LIG_14-3-3_CanoR_1	118	122	PF00244	0.459
LIG_14-3-3_CanoR_1	191	199	PF00244	0.541
LIG_14-3-3_CanoR_1	251	256	PF00244	0.452
LIG_14-3-3_CanoR_1	434	441	PF00244	0.724
LIG_14-3-3_CanoR_1	68	75	PF00244	0.452
LIG_14-3-3_CanoR_1	87	93	PF00244	0.360
LIG_APCC_ABBA_1	52	57	PF00400	0.365
LIG_BIR_III_2	469	473	PF00653	0.495
LIG_BRCT_BRCA1_1	100	104	PF00533	0.508
LIG_BRCT_BRCA1_1	437	441	PF00533	0.632
LIG_BRCT_BRCA1_1	48	52	PF00533	0.490
LIG_Clathr_ClatBox_1	92	96	PF01394	0.455
LIG_CSL_BTD_1	138	141	PF09270	0.475
LIG_CSL_BTD_1	290	293	PF09270	0.478
LIG_deltaCOP1_diTrp_1	133	139	PF00928	0.489
LIG_eIF4E_1	215	221	PF01652	0.453
LIG_eIF4E_1	340	346	PF01652	0.478
LIG_FHA_1	105	111	PF00498	0.458
LIG_FHA_1	309	315	PF00498	0.528
LIG_FHA_1	373	379	PF00498	0.409
LIG_FHA_1	49	55	PF00498	0.473
LIG_FHA_2	250	256	PF00498	0.529
LIG_FHA_2	403	409	PF00498	0.765
LIG_GBD_Chelix_1	321	329	PF00786	0.345
LIG_Integrin_isoDGR_2	224	226	PF01839	0.303
LIG_Integrin_RGD_1	467	469	PF01839	0.499
LIG_LIR_Apic_2	371	376	PF02991	0.398
LIG_LIR_Apic_2	84	89	PF02991	0.370
LIG_LIR_Gen_1	53	62	PF02991	0.361
LIG_LIR_Nem_3	107	112	PF02991	0.454
LIG_LIR_Nem_3	238	243	PF02991	0.454
LIG_LIR_Nem_3	337	343	PF02991	0.453
LIG_LIR_Nem_3	458	464	PF02991	0.506
LIG_LIR_Nem_3	49	55	PF02991	0.382
LIG_PCNA_PIPBox_1	129	138	PF02747	0.503
LIG_PDZ_Class_3	474	479	PF00595	0.357
LIG_Pex14_1	109	113	PF04695	0.340
LIG_Pex14_2	461	465	PF04695	0.385
LIG_SH2_CRK	245	249	PF00017	0.475
LIG_SH2_CRK	348	352	PF00017	0.453
LIG_SH2_CRK	90	94	PF00017	0.387
LIG_SH2_NCK_1	245	249	PF00017	0.539
LIG_SH2_STAP1	240	244	PF00017	0.453
LIG_SH2_STAP1	348	352	PF00017	0.453
LIG_SH2_STAP1	55	59	PF00017	0.390
LIG_SH2_STAT3	210	213	PF00017	0.514
LIG_SH2_STAT5	112	115	PF00017	0.453
LIG_SH2_STAT5	210	213	PF00017	0.464
LIG_SH2_STAT5	215	218	PF00017	0.441
LIG_SH2_STAT5	245	248	PF00017	0.500
LIG_SH2_STAT5	298	301	PF00017	0.464
LIG_SH2_STAT5	45	48	PF00017	0.467
LIG_SH2_STAT5	90	93	PF00017	0.530
LIG_SH3_3	213	219	PF00018	0.453
LIG_SUMO_SIM_anti_2	324	331	PF11976	0.473
LIG_SUMO_SIM_par_1	217	223	PF11976	0.539
LIG_TYR_ITIM	88	93	PF00017	0.362
MOD_CDK_SPxxK_3	372	379	PF00069	0.455
MOD_CK1_1	205	211	PF00069	0.457
MOD_CK1_1	23	29	PF00069	0.713
MOD_CK1_1	238	244	PF00069	0.467
MOD_CK1_1	249	255	PF00069	0.422
MOD_CK1_1	260	266	PF00069	0.464
MOD_CK1_1	297	303	PF00069	0.503
MOD_CK1_1	395	401	PF00069	0.750
MOD_CK1_1	41	47	PF00069	0.290
MOD_CK1_1	416	422	PF00069	0.628
MOD_CK1_1	423	429	PF00069	0.755
MOD_CK1_1	442	448	PF00069	0.612
MOD_CK1_1	50	56	PF00069	0.369
MOD_CK2_1	249	255	PF00069	0.556
MOD_CK2_1	377	383	PF00069	0.528
MOD_CK2_1	402	408	PF00069	0.735
MOD_CK2_1	50	56	PF00069	0.395
MOD_CMANNOS	136	139	PF00535	0.339
MOD_Cter_Amidation	224	227	PF01082	0.265
MOD_Cter_Amidation	432	435	PF01082	0.675
MOD_GlcNHglycan	179	182	PF01048	0.255
MOD_GlcNHglycan	192	195	PF01048	0.229
MOD_GlcNHglycan	36	39	PF01048	0.671
MOD_GlcNHglycan	40	43	PF01048	0.501
MOD_GlcNHglycan	418	421	PF01048	0.736
MOD_GlcNHglycan	425	428	PF01048	0.600
MOD_GlcNHglycan	441	444	PF01048	0.725
MOD_GlcNHglycan	445	448	PF01048	0.664
MOD_GSK3_1	113	120	PF00069	0.449
MOD_GSK3_1	131	138	PF00069	0.453
MOD_GSK3_1	20	27	PF00069	0.496
MOD_GSK3_1	294	301	PF00069	0.570
MOD_GSK3_1	34	41	PF00069	0.605
MOD_GSK3_1	390	397	PF00069	0.707
MOD_GSK3_1	408	415	PF00069	0.657
MOD_GSK3_1	416	423	PF00069	0.741
MOD_GSK3_1	435	442	PF00069	0.495
MOD_GSK3_1	46	53	PF00069	0.529
MOD_GSK3_1	6	13	PF00069	0.703
MOD_N-GLC_1	363	368	PF02516	0.253
MOD_N-GLC_1	403	408	PF02516	0.759
MOD_N-GLC_1	412	417	PF02516	0.692
MOD_N-GLC_2	271	273	PF02516	0.253
MOD_NEK2_1	104	109	PF00069	0.369
MOD_NEK2_1	113	118	PF00069	0.386
MOD_NEK2_1	190	195	PF00069	0.500
MOD_NEK2_1	220	225	PF00069	0.568
MOD_NEK2_1	308	313	PF00069	0.556
MOD_NEK2_1	321	326	PF00069	0.558
MOD_NEK2_1	377	382	PF00069	0.473
MOD_NEK2_1	46	51	PF00069	0.384
MOD_NEK2_1	6	11	PF00069	0.650
MOD_NEK2_1	98	103	PF00069	0.454
MOD_PIKK_1	243	249	PF00454	0.514
MOD_PKA_1	434	440	PF00069	0.635
MOD_PKA_1	68	74	PF00069	0.488
MOD_PKA_2	117	123	PF00069	0.448
MOD_PKA_2	190	196	PF00069	0.559
MOD_PKA_2	34	40	PF00069	0.611
MOD_PKA_2	434	440	PF00069	0.728
MOD_PKA_2	68	74	PF00069	0.433
MOD_PKA_2	99	105	PF00069	0.437
MOD_PKB_1	2	10	PF00069	0.511
MOD_Plk_4	135	141	PF00069	0.479
MOD_Plk_4	205	211	PF00069	0.487
MOD_Plk_4	238	244	PF00069	0.482
MOD_Plk_4	294	300	PF00069	0.544
MOD_Plk_4	41	47	PF00069	0.412
MOD_Plk_4	88	94	PF00069	0.377
MOD_Plk_4	99	105	PF00069	0.406
MOD_ProDKin_1	10	16	PF00069	0.652
MOD_ProDKin_1	235	241	PF00069	0.453
MOD_ProDKin_1	24	30	PF00069	0.617
MOD_ProDKin_1	363	369	PF00069	0.446
MOD_ProDKin_1	372	378	PF00069	0.363
MOD_ProDKin_1	420	426	PF00069	0.769
TRG_DiLeu_BaEn_1	156	161	PF01217	0.539
TRG_DiLeu_BaEn_1	459	464	PF01217	0.533
TRG_DiLeu_BaEn_2	456	462	PF01217	0.551
TRG_DiLeu_BaLyEn_6	216	221	PF01217	0.453
TRG_DiLeu_BaLyEn_6	356	361	PF01217	0.556
TRG_ENDOCYTIC_2	245	248	PF00928	0.475
TRG_ENDOCYTIC_2	340	343	PF00928	0.457
TRG_ENDOCYTIC_2	348	351	PF00928	0.446
TRG_ENDOCYTIC_2	55	58	PF00928	0.358
TRG_ENDOCYTIC_2	90	93	PF00928	0.360
TRG_ER_diArg_1	1	4	PF00400	0.672
TRG_ER_diArg_1	434	436	PF00400	0.682
TRG_ER_diArg_1	465	467	PF00400	0.443
TRG_ER_diArg_1	67	69	PF00400	0.556
TRG_Pf-PMV_PEXEL_1	158	162	PF00026	0.327
TRG_Pf-PMV_PEXEL_1	60	64	PF00026	0.454

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1A1	Leptomonas seymouri	67%	100%
A0A0S4ISF0	Bodo saltans	25%	84%
A0A0S4J6I6	Bodo saltans	39%	100%
A0A1X0NFL2	Trypanosomatidae	51%	100%
A0A1X0NRN8	Trypanosomatidae	31%	97%
A0A3Q8IDP1	Leishmania donovani	94%	89%
A0A422NE52	Trypanosoma rangeli	50%	100%
A0A422P136	Trypanosoma rangeli	26%	73%
A4H9E1	Leishmania braziliensis	22%	100%
A4HG29	Leishmania braziliensis	78%	94%
A4I351	Leishmania infantum	94%	100%
C9ZJM2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
E9ARH4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	100%
E9AZF8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
P38160	Sus scrofa	35%	100%
P38584	Bos taurus	34%	100%
P38585	Mus musculus	35%	100%
Q10438	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	23%	100%
Q23SI8	Tetrahymena thermophila (strain SB210)	26%	100%
Q8NG68	Homo sapiens	35%	100%
Q9QXJ0	Rattus norvegicus	34%	100%
V5ARK1	Trypanosoma cruzi	24%	100%
V5B4U2	Trypanosoma cruzi	24%	87%
V5D711	Trypanosoma cruzi	51%	100%